• 생약학회지
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• 제30권1호
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• pp.54-58
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• 1999

A method using coloric and spectrophotometric detection have been developed for the identification of the tertiary or quaternary alkaloids contained in Corydalis tuber and its preparations. The principle is based on the formation or decomposition of complex compounds. The complex compound of the tertiary and quaternary alkaloids have been formed by adding tetrathiocyanatocobaltate [II] ion to the test soln. Diverse crude drugs were screened using this method and the results indicated that isoquinoline, aconitine-type alkaloids in crude drugs can be readily detected. The method is simple, convenient, reproducible and applicable to the verification of the crude drug Corydalis tuber and its preparations.

• 생약학회지
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• 제21권4호
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• pp.284-289
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• 1990

Crude Aconiti Tubers were irradiated with microwave for 5 or 10 min with or without $NaHCO_3$. Ten min-irradiation reduced the contents of aconitine and mesaconitine to the level of those of commercially available processed Aconiti tuber power which is prepared with the high pressure-high temperature technique. The contents of benzoylaconine of the 10 min-irradiated Aconiti tubers were also comparable to those of commerciallized processed Aconiti tuber powder. However, irradiation with 1% $NaHCO_3$ soln. resulted in the drastic reduction of all the three above alkaloids.

• 대한한의학회지
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• 제32권5호
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• pp.126-133
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• 2011

Objective: We report a case of non-specific Aconiti Tuber poison complaining only of severe peripheral neurotoxicity without cardiac dysfunction. Methods: The authors evaluated the symptom changes of a patient who was hospitalized in an Oriental hospital for fourteen days. The patient received acupuncture, herbal medicine, moxibustion and analgesics. Result: No abnormality in examination for cardiac function or biochemical parameters was present. The severity of pain and dysesthesia in lower extremities gradually receded during the period of treatment with herbal and western medicines. Conclusion: This study provides helpful information for treatment of Aconiti Tuber toxicity.

• 한국자원식물학회지
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• 제28권3호
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• pp.305-311
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• 2015

This study investigated in vitro antioxidant, anti-inflammatory and cytotoxicity on human lung epithelial A549 cells of different solvent extracts from Jerusalem artichoke (Helianthus tuberosus L.) tuber. The EtOH extract contained amounts of phenolics (22.20 tannic acid equivalent ㎎/ɡ) and exhibited the highest antioxidant activity and anti-inflammatory activity. Several methods were employed for measure the antioxidant activity: 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity (IC₅₀ = 206.79 ㎍/㎖), reducing power activity (21.26 ascorbic acid equivalent ㎎/ɡ) and total antioxidant activity (19.05 ascorbic acid equivalent ㎎/ɡ). Meantime, the EtOH extract inhibited the NO production completely with a concentration of 800 ㎍/㎖. Besides, the H₂O extract exhibited more potent effect on human lung epithelial A549 cells. This study suggested that Jerusalem artichoke tuber had antioxidant, anti-inflammatory and cytotoxicity on human lung epithelial A549 cells.

• 한국자원식물학회지
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• 제7권1호
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• pp.63-66
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• 1994

The experiment was carried out to study the bothanical characteristics of Aconitum charmichaeli Sieb. et Zucc in Korea.The results obtained are summarized as follows ; maximum flowering date was 15 Sep. and plantheight was 65cm, stem diameter was 8.7mm, No. of node was 25 and node of flowering initiation was15th.In flowering, No. of anothers were 52 and No. of flowers were 49.In leaf, length and width was same size, lower leaf size was 2-3 times than upper leaf, lower leafarea was 8.4 times than upper leaf.In tuber, No. of tuber per plant was 8, fresh weight per tuber was 25.4g, total fresh weight per plantwas about 200g.

• 한국균학회지
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• 제23권1호통권72호
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• pp.10-13
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• 1995

자실체를 토양 내에 형성하는 자낭균의 일종인 덩이버섯 1 종(種)이 계룡산 국립공원에서 채집, 동정되었기에 보고하는 바이다. 동정된 종은 여름갈고리덩이버섯(Tuber aestivum subsp. uncinatum)으로 국내에서는 기재된 바가 없다. 자실체의 크기는 $3{\sim}10\;cm$ 정도이며, 혹모양으로 매우 불규칙적인 형태를 지니고 있다. 자낭에는 $1{\sim}6$개의 포자가 있으며, 포자표면은 침상돌기로 덮혀 있다. 상기의 표본은 건조 및 액침표본 상태로 대전대학교 미생물학과 표본실에 보관되어 있다.

• 한국균학회지
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• 제49권1호
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• pp.101-108
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• 2021

We collected the ascomata of Tuber species from the rhizosphere of Quercus dentata in Danyang, Korea. We observed the morphological characteristics of ectomycorrhizal roots and ascomata, and identified the species based on the results of the phylogenetic analysis conducted using the DNA sequences of an internal transcribed spacer, a large-subunit rDNA, translation elongation factor 1-α DNA (TEF1), and MAT. Finally, we identified the fungal species as Tuber himalayense B.C. Zhang & Minter, which has not been recorded previously in Korea. We evaluated the morphological characteristics and conducted phylogenetic analysis of the ascoma and mycorrhiza (associated with Q. dentata) of T. himalayense.

• Mycobiology
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• 제49권6호
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• pp.527-533
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• 2021

The truffle and ectomycorrhizal roots formed by Tuber sp. were collected from the rhizosphere of Quercus aliena in Korea. The morphological characteristics of the ascoma, and molecular phylogenetic analysis using sequences from the internal transcribed spacer (ITS) and large subunit (LSU) of ribosomal DNA, translation elongation factor 1-alpha (TEF), and RNA polymerase second largest subunit (RPB2) regions confirmed the distinct morphology of the truffle. This truffle belongs to a monophyletic clade among the other Tuber species in the phylogeny. This study describes the truffle, Tuber koreanum, as a new species reported from Korea.

• 한국균학회지
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• 제51권2호
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• pp.121-125
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• 2023

In this study, we examined the ectomycorrhiza (ECM) formed by a species of the genus Tuber, T. koreanum, with two indigenous Korean oak species, Quercus acutissima and Q. dentata. T. koreanum showed a high colonization rate for both oak species, with a higher mean colonization rate observed for Q. acutissima. The mycorrhizal characteristics of T. koreanum were described, revealing distinct morphological and anatomical differences between the ECM formed with the two host plants. This study is significant because it is the first to elucidate ECM formations of T. koreanum in indigenous oak trees. However, further studies are required to differentiate the ECM associations formed by T. koreanum from those of other Tuber species.

• Mycobiology
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• 제50권4호
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• pp.238-243
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• 2022

Members of the genus Tuber are ectomycorrhizal fungi; this genus includes more than 180 species worldwide. In the present study, the optimal pH, temperature, and medium suitable for the mycelial growth of the Korean truffle, Tuber koreanum, were determined. Mycelium of T. koreanum, isolated from fruiting bodies collected in Korea, was used to investigate the effects of these environmental factors. The results showed that malt extract agar and potato dextrose agar were the most suitable for the mycelial growth of T. koreanum when cultured at a pH of 6.0 at 25 ℃ for 30 days.