• Nutritional Sciences
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• v.3 no.1
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• pp.11-17
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• 2000

This study was intended to examine whether dehydroepiandrosterone (DHEA) and dietary fat level or source could modulate glutathione utilizing detoxifying system activity and the cytosolic NADPH generation in rat liver. Male Sprague-Dawley rats were fed semipurifed diet containing either 2%(w/w) corn oil (low level of corn oil diet: 5 ca% of fat) 15% corn oil (high level of corn oil diet: 31 cal% of fat) or 13% sardine oil plus 2% corn oil(high level of fish oil diet: 31 cal% of fat) for 9 weeks. Half of the rats in each diet group were fed a diet supplemented with 0.2% DHEA (w/w). DHEA administration increased plasma total cholesterol level in low corn oil diet-fed rats. The high fish oil diet significantly decreased plasma total cholesterol level compared to the high corn oil diet. Plasma triglyceride level was not significantly changed by DHEA administration and dietary fat level and source. Fasting plasma glucose level was increased by DHEA administration and fish oil diet. Glucose 6-phosphate dehydrogenase activity in liver tissue was significantly increased by DHEA administration and high fat diet, especially fish oil diet. Malic enzyme activity in liver tissue was significantly increased by DHEA administration and high fat diet, especially fish oil diet. Malic enzyme activity in liver tissue was significantly increased by DHEA administration. DHEA suppressed the glutathione peroxidase, glutathione-dependent enzymes compared to the low corn oil diet, while fish oil diet elevated the activity of glutathione peroxidase and glutathione reductase compared to corn oil diet. These results suggest that DHEA administration and high level of corn oil diet may suppress the cellular detoxifying system activity through reduction of glutathione utilization, while the fish oil diet did not show these effects.

• Journal of agricultural medicine and community health
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• v.22 no.1
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• pp.27-34
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• 1997

This study was performed to offer the fundamental data for preventing and controlling the cardiovascular diseases of rural community women. The number of 168 women aged 40 to 50s who live in Kumsan-gun, Chungnam were selected for analysis after medical examination by a Kumsan-gun health center during the period of November to December in 1996. Total cholesterol(TC), triglyceride(TG), high density lipoprotein cholesterol(HDL-C), blood pressure(BP), degree of obesity(BMI) and atherosclerosis index(AI) were measured and relation between these physical measurements to serum lipid levels were studied. The results were as follows; 1. Mean TC level, TG level, BP, BMI and AI were significantly increased with advancing age. 2. Mean TC level, TG level, BMI and AI of borderline BP group and hypertension group were significantly increased than those of normal BP group. 3. Mean TC level, TG level and AI of obesity group were significantly increased than those of normal BMI group. 4. Simple correlation analysis showed that the positive correlation between TC, TG, BP, BMI and AI, but the level of HDL-C was negative correlation with TG and AI. 5. In multiple regression analysis taking HDL-C level as the dependent variable and TG, TC, BP, BMI, Age AI as the independent variable, AI, TG, BMI, TC were significantly related to HDL-C.

• Journal of Korean Medicine Rehabilitation
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• v.15 no.2
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• pp.1-16
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• 2005

Objectives : This experimental study was designed to investigate the effects of EjinTang-Gamybang(Erchentang-jiaweifang ; ETG) on the change of some values related to obesity and observe the complications coming from obesity in obese rat induced by high fat diet. Methods : Experimental group were as follows ; normal group were fed normal diet and administered DDW $1.0m{\ell}$ to rat during 7 weeks, control group were fed high fat diet and administered DDW $1.0m{\ell}$ during 7 weeks, sample A were fed high fat diet and administered ETG 500 mg/kg $1.0m{\ell}$ during 7 weeks, sample B were fed high fat diet and administered ETG 700 mg/kg $1.0m{\ell}$ during 7 weeks. Results & Conclusions : 1. Sample A and Sample B significantly decreased body weight, serum LDL-cholesterol level, serum free fatty acid level, serum total lipid level, serum phospholipid level and serum leptin level in comparison with control group. 2. Sample B significantly decreased serum total cholesterol level and serum triglyceride level in comparison with control group. 3. Sample B significantly increased serum HDL-cholesterol level in comparison with control group. According to above results, the author suggested that ETG was able to be used for the herbal medication of obesity. 4. ETG significantly increased rCBF, and increased CMF in a dose-dependent. 5. ETG significantly decreased MABP in a dose-dependent. 6. rCBF was significantly and stably increased by ETG(10 mg/kg, i.p.) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in control group. This results were suggested that ETG significantly increased rCBF by dilating arterial diameter and activating serum leptin level. So that, the present author thought that ETG had an effects of obesity and complication coming from obesity(ischemic cerebral and cardiac disease).

• Journal of Nutrition and Health
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• v.35 no.7
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• pp.743-753
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• 2002

The purpose of this study was to investigate the relationship among nutritional status, serum lipids and leptin of 134 elementary school students with different obesity index residing in Kyunggi-do, Korea. Subjects were assigned to under weight, normal weight or over weight group according to the their obesity index. Their nutritional status, serum lipid and leptin levels were evaluated based on 24-hr dietary recalls, anthropometric measurements and blood analysis. The mean weight, height and obesity index of total subjects was 150.8cm, 45.6kg and 5.4%. The mean % of body fat was higher while LBM and TBW (total body water) were lower in over weight subjects than those of other two groups. Total food, plant protein, carbohydrate, and vitamin C intakes of underweight male students were lower than those of overweight male students. The consumption of total fat was the highest in the female normal weight subjects and the lowest in female overweight subjects. Male students consumed more plant protein and vitamin C than female students. The mean daily intake of vitamin B$_2$, calcium and zinc of all subjects did not meet the Korean RDA. Also, iron intake of female students was lower than the Korean RDA. The mean level of serum leptin was the highest in male and female overweight subjects (p＜0.001). The HDL-cholesterol level was the highest in the underweight group of male subjects (p＜0.05) and in the normal weight group of female subjects (p＜0.001) The serum level of leptin and atherogenic index (AI) of females were higher than those of males. Serum leptin of total subjects had significantly positive correlation with obesity index, weight, SBP, body fat, cholesterol intake, serum triglyceride, and AI. However, serum leptin was negatively correlated with LBM, TBW, intake of plant protein, vitamin B$_1$, vitamin C and serum HDL-cholesterol. Obesity index was also positively correlated with weight, SBP, body fat, serum triglyceride and AI. To summarize the results, the vitamin B$_2$, calcium, zinc, iron and fiber intakes should be supplied to elementary school students, especially those who were overweight. The average serum leptin level of students showed remarkably positive correlation with obesity index, body fat, serum lipids and AI. Also, it was suggested that the serum leptin might be related to the dietary factors like cholesterol, plant protein, vitamin B$_1$ and vitamin C of elementary school students.

• Preventive Nutrition and Food Science
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• v.11 no.1
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• pp.48-53
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• 2006

Although garlic has been reported to have impressive effects in lowering serum lipids, there have been controversial evaluations on these effects. To find the potential fator causing the inconsistency in the previous studies, we examined the effects of two types of garlic according to the producing-area (hangihyung garlic, nangihyung garlic) on serum lipid profiles and leptin level. Thirthy six of 4 wk old Sprague Dawley male rats fed high fat diet (40% of calories as fat) for 6 wks to induce obesity, and subsequently fed 5% garlic powder supplemented (HF+H: hangihyung garlic powder, HF+N: nangihyung garlic powder) high fat diets (w/w) for further 5 wk. For the comparison, normal control group fed AIN-76A diet (11.7% of calories as fat). Supplementation with hangihyung and nangihyung garlic resulted in a significant reduction of high fat induced body weight gain, white fat (i.e., epididymal, visceral and peritoneal fat) development, adipocyte hypertrophy and the development of hyperinsulinemia and hyperliptinemia. Serum triglyceride and total cholesterol level was greatly reduced by hangihyung garlic supplementation (p<0.05). The HDL-cholesterol level was increased by dietary hangihyung and nangihyung garlic. There were slight non-significant decreases in triglyceride and total cholesterol of HF+N group as compared to those of HF group. Leptin level of HF+H group was found to be significantly lower than HF group (p<0.05). There was no significant difference among N group and HF+N group. These results suggest that hangihyung garlic may lead to the higher activity in improving lipid profiles than nangihyung garlic. Whether the hypolipidemic effect of garlic increases in a species-dependent has yet to be determined and awaits further research.

• The Korea Journal of Herbology
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• v.27 no.2
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• pp.69-75
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• 2012

Objectives : We investigated the effects of mountain cultivated ginseng water boiled extract(MCG) on blood glucose and insulin levels, and examined whether lipid metabolism are improved by it in male db/db mice(a murine model of type 2 diabetes mellitus). Methods : 9 weeks old, male db/db mice were divided into 5 groups : C57BL/6J normal, control, MCG-250mg/kg (MCG-1), MCG-500mg/kg(MCG-2) and MCG-1000mg/kg(MCG-3). After mice were treated with MCG for 8 weeks, we measured body weight, food intake, fat weight, visceral organ weight and blood glucose, insulin and lipid levels. Results : 1. We found no difference in body weight, food intake, fat weight and visceral organ weight among the animal groups. 2. Compared with controls, MCG-treated mice had lower blood glucose level and higher blood insulin levels, the magnitude of which was prominent in MCG-2. 3. Compared with controls, MCG-treated mice had lower LDL-cholesterol and higher HDL-cholesterol levels. 4. Compared with controls, MCG-treated mice had blood triglyceride and free fatty acid levels, the magnitude of which was prominent in MCG-2. 5. Blood AST and ALT concentrations were not changed by MCG, indicating MCG do not show any toxic effects. Conclusions : These results demonstrate that MCG effectively increases blood insulin level and decreases blood glucose level, blood lipid levels, and prevents and improves diabetic dyslipidemia and cardiovascular disease.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.12
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• pp.1760-1764
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• 2002

In order to elucidate the physiological function of circulating IGF-I on muscle protein synthesis in the chicken under malnutritional conditions, we administrated recombinant chicken IGF-I using a osmotic mini pump to fasted young chickens and measured the rate of muscle protein synthesis and plasma metabolite. The pumps delivered IGF-I at the rate of $22{\mu}g/d\{300{\mu}g{\cdot}(kg\;body\;weight{\cdot}d)^{-1}\}$. Fractional rate of protein synthesis in the muscle was measured using a large dose injection of L-[$2,6-^3H$]phenylalanine. Constant infusion of chicken IGF-I did not affect plasma glucose level. Significant interaction between dietary treatment and IGF-I infusion was observed in plasma NEFA and total cholesterol concentrations. When chicks were fasted, IGF-I infusion decreased plasma NEFA and total cholesterol concentrations. On the other hand, IGF-I administration did not affect plasma levels of both metabolites. Fasting reduced plasma triglyceride concentration significantly. IGF-I infusion also decreased the level of plasma triglyceride. Plasma IGF-I concentration of young chickens was halved by fasting for 1 d. IGF-I infusion using an osmotic minipump for 1 d increased plasma IGF-I concentration in fasted chicks to the level of fed chicks. Fasting decreased body weight and the loss of body weight was significantly ameliorated by IGF-I infusion. There was a significant interaction between dietary treatment and IGF-I infusion in the fractional rate of breast muscle protein synthesis. There was no effect of IGF-I infusion on muscle protein synthesis in fed chicks. Muscle protein synthesis reduced by fasting was ameliorated by IGF-I infusion, but did not reach to the level of fed control. Muscle weight of fasted chicks infused with IGF-I was similar to fasted birds without IGF-I infusion, which suggests that muscle protein degradation would be increased by IGF-I infusion as well as protein synthesis in fasted chicks.

• The Journal of Internal Korean Medicine
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• v.20 no.1
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• pp.181-197
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• 1999

In order to investigate the protective effect of Samduhaejungtang-gamibang on the liver injury of rats induced by $CCl_4$ and d-galactosamine, the serum transaminase(GOT& GPT) alkaline phosphatase(ALP), lactic dehydrogenase(LDH) for enzyme activities and triglyceride, total bilirubin amounts for serum component were measured. All animals were divided into 4 groups, those were normal group(untreated), control group(treated with vehicle 0.9% Saline solution), sample Ⅰ group(1500mg/kg administrated), sample Ⅱ group(3000mg/kg administrated). The results were as follows: 1. The inhibitory effects of the serum GOT activities in rats induced by $CCl_4$ were noted in sample Ⅱ group(p<0.01). In serum GPT activities, sample Ⅱ group(p<0.05) only showed the inhibitory effects. 2. The inhibitory effects of the serum ALP activities in rats induced by $CCl_4$ were noted in sample Ⅱ group(p<0.01) 3. The inhibitory effects of the serum LPH activities in rats induced by $CCl_4$ were noted in sample Ⅱ group, but it is not recognized statistically. 4. The increases effects of the serum triglyceride content level in rats induced by $CCl_4$ were inhibited in both sample Ⅰ group(p<0.05) and sample Ⅱ group(p<0.01) 5. The increases effects of the serum total bilirubin content level in rats induced by $CCl_4$ were inhibited in sample Ⅱ group(p<0.05) 6. The inhibitory effects of the serum GOT, GPT activities in rats induced by d-galactosamine were noted in sample Ⅱ group (p<0.001), but sample Ⅰ group was not recognized. 7. The signiticantly inhibitory effects of in the serum LDH activities in rats induced by d-galactosamine were noted in both sample Ⅰ group(p<0.05) and sample Ⅱ group (p<0.001) 8. The increases of the serum ALP content level in rats induced by d-galactosamine were inhibited in sample Ⅱ group(p<0.05) 9. The increases of the serum total bilirubin content level in rats induced d-galactosamine were inhibited in sample Ⅱgroup(p<0.05) According to the above results, it is considered that Samduhaejungtang-gamibang has protective effect against liver injury in rats induced by $CCl_4$ and d-galactosamine. So it is required to study about the actions of mutual relation of medicines and patho-mechanism by experiment.

• Food Science of Animal Resources
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• v.26 no.2
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• pp.175-182
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• 2006

Diets consist of two different pork samples: pork of a Jeju native pig ( 260 days old, $101{\sim}103kg$ ) not fed tangerine byproduct during finishing period ($T_0$), and pork fed 8% and 15% tangerine byproduct during growing and finishing period ($T_1$), respectively. The effects of the diet on the physiological activities of rats were studied by feeding 17-week old rats with the two diets for 4 weeks. There was no significant difference between $T_0$ and $T_1$ in the rat's feed intake, feed efficiency ratio, and weight gain. Furthermore, there was no significant difference between $T_0$ and $T_1$ in the rat's weight of liver, kidney, spleen, epididymal fat pad, triglyceride and cholesterol of liver. Both $T_0$ and $T_1$ showed similar trends in terms of total lipid, phospholipid, triglyceride, total cholesterol, atherogenic index, protein, glucose, hemoglobin level, mineral level, and ${\gamma}$-GTP, ALT, AST and ALP activities. However, $T_1$ showed the trend of increasing amount of the serum's HDL and LDL cholesterol level, compared with $T_0$.

• Journal of Korean Medicine Rehabilitation
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• v.18 no.4
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• pp.73-83
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• 2008

Objectives : In this research, it was investigate that had effects on acupuncture techniques of $HT_{8}$(Shaofu) and $LR_{2}$(Xingjian) acupoints in high fat rats. We recorded data of weight gain, food intake, food efficiency, total cholesterol, triglyceride, AST, ALT, r-GTP, total bilirubin on rats fed high fat diet for 10 weeks. Methods : We divided experimental groups into four. Group of high-fat diet feeding and no treatment was control, group of high-fat diet feeding and acupuncture at $HT_{8}{\cdot}LR_{2}$ was Acu-1, group of high-fat diet feeding and acupuncture at $HT_{8}{\cdot}LR_{2}$ and rotated counter-clockwise 36times was Acu-2, high-fat diet feeding and acupuncture at $HT_{8}$(body direction)${\cdot}LR_{2}$(lower limb direction) and rotated counter-clockwise 36times was Acu-3. Results : In Acu-3 group, body weight on 2 weeks after starting acupuncture, food efficiency and the level of serum total cholesterol were decreased, and the level of HDL-cholesterol was increased. In Acu-1 group, the level of triglyceride was increased but ALT was decreased. Conclusions : These results suggest that treatment of acupuncture at $HT_{8}$(body direction)${\cdot}LR_{2}$(lower limb direction) and rotated counter-clockwise 36times may be having the influence on high fat by controlling the level of serum total cholesterol and HDL-cholesterol.