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Isolation, Identification and Mutant Development of Butanol Tolerance Bacterium (부탄올 내성 미생물의 분리, 동정 및 변이주의 개발)

  • Jung, Hyesook;Lee, Jinho
    • Microbiology and Biotechnology Letters
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    • v.41 no.1
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    • pp.26-32
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    • 2013
  • Butanol-resistant bacteria were isolated from butanol solvent. The cell growth of isolated strains declined with increasing concentrations of butanol, and isolated strain BRS02 displayed more resistance to 12.5 g/L of butanol than other isolated strains. In addition, strain BRS251, which was resistant to even higher concentrations of butanol, was developed by the mutation of BRS02 using UV. BRS251 could grow in LB medium containing up to 17.5 g/L of butanol, 32.5 g/L of propanol, or 6 g/L of pentanol, whereas the control strain Escherichia coli was found to be tolerant to 7.5 g/L of butanol, 20 g/L of propanol, or 2 g/L of pentanol. The isolated BRS02, a Gram(+) bacterium seen to have a cocci form under the microscope, grew in 6.5% NaCl. According to biochemical tests, BRS02 can metabolize and produce acid with D-galactose, D-maltose, D-mannitol, D-mannose, methyl-${\beta}$-Dglucopyranoside, D-ribose, sucrose, or D-trehalose, as carbon sources. Also, this strain showed resistance to bacitracin, vibriostatic agent O/129, and optochin, alongside positive activities for arginine dihydrolase, ${\alpha}$-glucosidase, and urease. The BRS02 strain was identified as Staphylococcus sp. by analyses of the 16S rRNA gene, phylogenetic tree, and biochemical tests.

Study on the Ecological Restoration of Rock-exposed-cut-slope by Natural Topsoil Restoration Methods : In Case of Won-Ju Experiment (자연표토 복원공법에 의한 암절취비탈면의 생태적 복원에 관한 연구 : 원주사례지역을 중심으로)

  • Nam, Sang-Joon;Suk, Won-Jin;Kim, Nam-Choon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.2 no.4
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    • pp.54-63
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    • 1999
  • This study was conducted to suggest the ecological restoration and environmentally friendly revegetation technology for the rock-exposed cut-slopes by the Natural Topsoil Restoration Methods (NTRM) with the following restoration objectives; (1) prevention or reduction of wind and water erosion, (2) provision of food and cover for variety of animal species, (3) improvement of the visual or aesthetic quality of the disturbed slopes. On Nov. in 1995, the 5cm thick layer of artificial soil and 2cm thick layer of straw-mulching was attached at rock-exposed cut-slopes by NTRM without using anchor wire and anchor pin. The main results during four years surveying on the ground-coverage effect, plant growth, species diversity and importance values were summarized as follows. 1. Artificial soil attached at rock exposed cut-slopes was not eroded until recovered by tree and herbaceous vegetation in spite of not using anchor wire and anchor pin. Also it shows low soil hardiness and has almost the same amount of bacteria and fungi with in surrounding natural topsoil. 2. In 'combination for the woody vegetation', Lespedeza cyrtobotrya, Albizzia julibrissin, Rhus chinensis, Indigofera pseudo-tinctoria occupied upper layer vegetation. Since three years after seeding, Indigofera pseudo-tinctoria had overwhelmed the other woody plants and cool season foreign grasses, Erigeron canadensis, Taraxacum mongolicum, Commelina communis, Arundinella hirta (Thunberg) and Oenothera erythrosepala grows at lower part of the vegetation, 3. The heights of the Rhus chinensis grows 1.8m, Indigofera pseudo-tinctoria 2.0m, so it seems that the objectives of woody vegetation with native plants could be accomplished. 4. After 4 years later after seeding in 'combination for the herbaceous vegetation', the most dominant plant was Indigofera pseudo-tinctoria, the next was in order of cool-season grasses, Taraxacum mongolicum, Erigeron canadensis, lxeris dentata (Thunberg), Oenothera erythrosepala, Arundinella hirta (Thunberg). The diversity index in 'combination for woody vegetation' was higher than that in 'combination for the herbaceous vegetation'. The tendency of the intrusion of secondary succession plants was more effective in 'combination for the herbaceous vegetation' than in 'combination for the woody vegetation'.

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Habitat Characteristics and Distribution of Cymbidium kanran Native to Jejudo, Korea (제주한란의 자생환경 특성 및 분포에 관한 연구)

  • Lee, Jong Suk
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.7 no.1
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    • pp.40-49
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    • 2004
  • This work carried out to define the characteristic of Cymbidium kanran habitat at Mt. Halla in Jejudo, Korea from the ecological point of view including geological and topographical features, air and soil temperature, relative humidity, fluctuations of light intensity, habitat vegetation, distribution altitude, area limit, and etc. And another goal of this study consider the conservation counterplan of the cymbidium habitat. Natural distribution areas of the cymbidium were observed more abundantly on the well drained south and east-facing slopes. Soil acidity was ranged from pH 4.1 to 5.3, and electric conductivity was ranged from 176.4 to 299.9 us/cm (average 215.3 us/cm). Base-saturation percentage of the habitat soil was below 50%, bulk density 0.42g/$cm^2$, particle density 2.05g/$cm^2$, humus content 26%, total nitrogen 0.82%, available phosphate 4.2 mg/kg, exchangeable potassium 0.63 Cmol/kg, calcium 0.44 Cmol/kg and magnesium 0.67 Cmol/kg. Annual mean air temperature was $15.4^{\circ}C$, however, air temperature was ranged $11.7{\sim}18.2^{\circ}C$ in spring, $21.2{\sim}23.8^{\circ}C$ in summer, $12.8{\sim}22.0^{\circ}C$ in fall and $5.5{\sim}7.8^{\circ}C$ in winter season. Annual mean soil temperature at depth of 10cm was $13.2^{\circ}C$ And minimum value was recorded $4.7^{\circ}C$ on January, and maximum value $22.5^{\circ}C$ on August. Relative humidity was ranged 90.8~94.7% in summer, 80.8~91.5% in fall and 77.6~84.2% in winter season. Minimum value was 56.5% on December, and maximum value was 100% on July and August. Light intensities were ranged from 400 to 1,800 lux at the greater part of Cymbidium kanran sites in Jejudo. Summer regarded as an lower light intensities was recorded to be range of 500~600 lux; however, autumn and winter were shown higher light regimes ranged from 3,500 to 3,800 lux. Therefore, one must be suprised that the cymbidium grow at the light condition of 6 lux (minimum) or 10,000 lux (maximum). Tree species keeping higher frequency rate and density were Eurya japonica, Camellia japonica, Castanopsis cuspidata, Carpinus laxiflora and Pinus densiflora. Number of trees growing in a 5${\times}$5m quardrat was 35 as an average, and proportion of evergreen versus deciduous was 5:1. Distribution altitude of the orchid habitat was ranged from 120m (low) to 840m (high) from sea level on the south facing slope of Mt. Halla, and was ranged eastern borderline of Gujwaup, Bukjejugun to western boundaries of Jungmundong, Seogwipo city. For the stable conservation of Cymbidium kanran habitat, sunlight regimes must be increased more by means of cutting trees or twigs in the site.

Effect of Shade Net on Reduction of Freezing Damage at a Tea Garden (차밭에 설치된 차광망의 동해경감 효과)

  • Hwang, Jung-Gyu;Kim, Yong-Duck
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.16 no.2
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    • pp.146-154
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    • 2014
  • In the result of effect on freezing damage reduction by the shade net colors and the shade rate to tea trees during wintering period, the high shade ratio decreased tea trees growth and increased freezing damage and 55% of shade ratio based on non treatment developed new leafs and green leaf productivity. By the shade net colors, colorless shade net (55% of shade type) treatment and green shade net treatment increased green leaf productivity and decreased damaged area compare to non treatment shade net and black shade net treatment. Colorless net shade treatment reduced over 50% of freezing damage and increased (10a) 68 kg for green leaf productivity compare to non treatment shade net. The colors of shade net treatments for reduction freezing damaged in order of Black < Green < Colorless but reduction of freezing damage was so high. In the relative microclimates, treatment shade nets were $0.7^{\circ}C$ lower, average relatively humidity 14.9% higher, soil temperature $0.6^{\circ}C$ lower and soil moisture 4.6% higher than non treatment shade net. And treatment shade nets decreased average wind speed 0.7 m/s and it showed us treatment net shades effected to excessive evapotranspiration and soil dry by wind and considered one of good solution for freezing damages.

Seasonal Change of Cambium Activity of Pine Trees at Different Growth Sites (생장지역에 따른 소나무 형성층 활동의 계절적 변화)

  • Park, So-Yeon;Eom, Chang-Deuk;Seo, Jeong-Wook
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.4
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    • pp.411-420
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    • 2015
  • Despite of the same species there is a difference in the tree shape and its wood property due to quantitatiVe and qualitatiVe differences in wood cells by the intra-annual cambial actiVity. The purpose of this study was to proVide the fundamental database of intra-annual cambial seasonal actiVity for red pine in Sokwang-ri and Anmyondo for the determination of their wood qualities. As the results of intra-annual cambial actiVity, the initiation of cambial actiVity of red pine in the plots with Age Class V in Sokwang-ri was in the middle of April and it was at the beginning of April in all plots in Anmyondo. Only the plots with Age Class IX in Sokwang-ri showed relatiVely late initiation of cambial actiVity as between middle of May and middle of June. Except one plot with Age Class IX in Sokwang-ri all plots in both sites showed the same cessation of cambial actiVity as between middle and end of October. According to intra-annual cambial actiVity, the duration of cambial actiVity in Sokwang-ri is shorter than that of Anmyondo. On the other hand, the number of cell diVision of cambial actiVity in Sokwang-ri was more than those of Anmyondo. We could establish the database for intra-annual cambial actiVity for Sokwang-ri and Anmyondo to estimate their wood quality.

Characteristic of a new variety Lentinula edodes, 'Nongjin-go' (표고 톱밥재배용 신품종 '농진고' 특성)

  • Moon, Ji-Won;Lee, Chan-Jung;Cheong, Jong-chun;Kong, Won-Sik;Kim, Kil-ja
    • Journal of Mushroom
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    • v.13 no.3
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    • pp.228-232
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    • 2015
  • 'Nongjin-go' is a new breed strain of Lentinula edodes, saw-dust bag variety. It is a cross combination of dikaryon Lentinula edodes ASI 3305mut and monokaryon L5-16 of L. edodes ASI 3305(Sanjo701ho). We crossbred them by 2011 and verified productive capacity from 2012 to 2013 in Rural Development Administration. Optimum temperature of mycelial growth is $30^{\circ}C$ and it of fruit-body primodium formation is range from 15 to $23^{\circ}C$. Nongjigo is agricultured at mid-high temperature well. Fruiting body is platy-hemisphere, light brown and centralizing. And bast is formed around edge of pileus. Yield productions per period is regular than 'Sanjo701'. Plastic bag culture medium is 1.5 kilogram and culture periods are 90~100 days. As its browning of pileus in culture is a little slow, Light and ventilation is needed a lot in light-culturing. Humidity is controlled properly for its color in fruit-body growing. Tested culture medium is consisted of 80% Oak-Tree saw-dust and 20% rice-bran.

Medium development of Flammulina velutipes by using herb medicine refuse (폐 한방슬러지를 이용한 팽이버섯의 배지개발)

  • Seo, Kwon-Il;Kim, Chul-Ho;Seo, Dong-Cheol;Yee, Sung-Tae;Park, Kyung-Wuk;Lee, Chang-Yun;Lee, Sang-Won
    • Journal of Mushroom
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    • v.12 no.1
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    • pp.17-23
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    • 2014
  • pH of oriental medicine sludge was 5.3, which was similar to 5.2 of the main ingredient, corncob. Its sugar content, however, was 4.8 mg/g, which was 2.5 times higher than concorb's 1.9 mg/g. According to the addition content analysis of oriental medicine sludge by using blood agar plate, the experimental group showed much more robust growth than the control group. 10% of oriental medicine sludge was added to corncob and pine tree sawdust for test-tube culture. Then they were cultivated at $25^{\circ}C$ for 6 days after inoculating Flammulina velutipes liquid spawn. The control group and experimental group showed 2.2~3.4 and 5.8~6.4 cm hypae growths respectively. At the field test for 10% herbal medicine refuse, mushroom yield dropped by 5% compared to the control group. However, it had distinctively lower number of deformity and the 2nd grade products. An economic analysis was conducted based on the cultivation facility that produces 160,000 mushrooms per day. The analysis demonstrated that the facility can save 50,000,000 won in the starting year and 130,000,000 won in the following years from the unit cost of production excluding labour and operation cost.

A Study on the Method for Ecological Restoration on Abandoned Concrete-paved Road - Focused on the Experimental Construction Site in Young Dong Province of GyungBu Express Highway(227.24~229.04km) - (콘크리트 폐도의 생태복원 방안 모색에 관한 연구 - 경부선 영동군 황간지역 시험시공지를 중심으로(경부고속도로 227.24~229.04km 지점) -)

  • Kim, Nam Choon;Ann, Phil Gun;No, Su Dae;Kim, Do Hee
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.15 no.4
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    • pp.119-132
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    • 2012
  • The unmanaged abandoned concrete roads are vulnerable toward the issues on soil and water pollution, which requires flexible managing method such as eco-corridor after the process of ecological restoration. Among various alternations of abandoned concrete-paved roads, ecological restoration technique may be the most suitable method in sites including high quality of natural environment. Therefore, as in Young dong province, GyungBu express highway (227.24~229.04km), which is near to Hwang-gan IC, the survey to measure its effect of soil under the paving and water pollution by abandoned concrete roads was discussed. Then, the restoration method of plantings of landscape trees and hydro-seeding methods of artificial soil media was appraised through consecutive monitoring. The soil adequacy analysis shows lower percentage of heavy metal substance in each depth level compared to standard limit stated by the Ministry of Environment, along with low concerns raised after the analysis on heavy metal content of the spilled water on the concrete roads. Meanwhile, Korean Weigela (Weigela subsessilis L.H. Baily) was found to be withered in small-scale landscape trees planting sites. Among the seeding plants. the family of leguminosae, Silene armeria, Dendranthema boreale, Caryopteris incana and Aster yomena show good establishment results. Overall studies on planting of small and large landscape trees, planting method of container plants, planting method of ground cover plants, and germination and development trend of seeding plants of the experimental restoration site on abandoned concrete roads are revealing specific trends in the way landscape woody plants establishment and growth. Finally, this study suggests further studies and survey on varied plant restoration methods on abandoned concrete-roads for developed design guidelines of their methods.

Analysis of Water Retention Capacity at Sasa borealis Stands in Jirisan National Park (지리산국립공원 내 조릿대 임분의 수원함양기능 분석)

  • Ji, Hyung Woo;Park, Jae Hyeon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.11 no.3
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    • pp.1-11
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    • 2008
  • Although landslides were frequently occurred under Tripterygium regelii and Rubus sp. vegetations, the damage of landslide was not observed in sasa (Sasa borealis) stands. These phenomena may be affected by forest vegetation types. This result suggested that the landslide occurred in Jirisan (Mt.) National Park may be closely related to water retention capacity at Sasa borealis stands. This study compared and analyzed the water retention capacity of each soil horizon of sasa, larch (Larix leptolepis) and mongolian oak (Quercus mongorica) stands. Soil bulk density in A horizon was lower in sasa (0.776g/$cm^3$) than in mongolian oak (0.828g/$cm^3$) and in larch stands (1.282g/$cm^3$). Water permeability in A horizon was 0.02055cm/sec for sasa, 0.00575cm/sec for mongolian oak, and 0.0007cm/sec for larch stands, respectively. The water permeability of sasa stand was about 3.6 times and about 29 times higher than in mongolian oak and in larch stands, respectively. This result indicates that water infiltration of soil surface during a rain event is more rapid in sasa than in other two stands. Soil organic matter content in B horizon was lower in larch (0.7%) than in mongolian oak (6.5%) and in Sasa (3.3%) stands. The solid ratio in A horizon was highest in larch among three stands, but that of mongolian oak and larch stands showed a similar rate. Pore space rates was 70.7% for A horizon and 70.6% for B horizon of sasa, 68.9% for A horizon and 70.6% for B horizon of sasa, 68.9% for A horizon and 70.6% for B horizon of mongolian oak forests and 51.7% for A horizon and 49.2% for B horizon of larch forests, respectively. According to pore space rates, the water retention capacity may be poor in larch stand compared with other two stands. Soil strength in sasa and mongolian stands was over 25kgf/$cm^2$ from 40cm depth, while the strength was over 25kgf/$cm^2$ from 25cm depth in larch stand. The result indicates that tree growth and water permeability in larch stand could be limited due to high soil strength. Larch stand was poor for soil pore space development to be offered to the water retention capacity, but water retention capacity of A horizon soil in sasa stand was high than that of other two stands. Therefore, establishment of sasa stand under larch stand could help to prevent landslides.

Effects of Number of Seeds per Hill in Dibbling on Growth Character, Yield and Feeding Value in Jeju Native Sorghum (제주재래수수의 점파립수에 따른 사료수량 및 조성분 분석)

  • 조남기;강영길;송창길;전용철;오장식;조영일;박성준
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.24 no.2
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    • pp.129-136
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    • 2004
  • Jeju native sorghum was grown at six spot seeding rates (1, 2, 3, 4, 5 and 6) from April 3 to september 6, 2000 in Jeju island to determine influence of number of seeds per hill in dibbling on agronomic characters, forage yield and qualities. Days to heading was increased as number of seeds per hill was increased. Plant height with three seeds per hill (206.7 cm) was the longest, while with six seeds per hill (175.2 cm) was the shortest. Fresh forage, dry matter and crude protein yield and total digestible nutrient (TDN) were the greatest at the three seeds per hill (48.1, 10.1, 0.9, 5.1 MT/ha). Crude protein, crude fat and nitrogen-tree extract (NFE) increased with the number of seeds per hill increased but crude fiber and crude ash decreased.