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Medium development of Flammulina velutipes by using herb medicine refuse

폐 한방슬러지를 이용한 팽이버섯의 배지개발

  • Seo, Kwon-Il (Dept. of Food and Nutrition, Sunchon National University) ;
  • Kim, Chul-Ho (Dept. of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Seo, Dong-Cheol (Dept. of Bio-Environmental Sciences, Sunchon National University) ;
  • Yee, Sung-Tae (Dept. of Biology, Sunchon National University) ;
  • Park, Kyung-Wuk (S&J Bio Co., Ltd.) ;
  • Lee, Chang-Yun (Greenpeace Mushroom Institude of Green Co., Ltd.) ;
  • Lee, Sang-Won (Dept. of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
  • 서권일 (순천대학교 식품영양학과) ;
  • 김철호 (경남과학기술대학교 제약공학과) ;
  • 서동철 (순천대학교 생물환경학과) ;
  • 이성태 (순천대학교 생물학과) ;
  • 박경욱 (에스엔제이바이오(주)) ;
  • 이창윤 (그린합명회사 그린피스 버섯연구소) ;
  • 이상원 (경남과학기술대학교 제약공학과)
  • Received : 2014.03.05
  • Accepted : 2014.04.02
  • Published : 2014.03.31

Abstract

pH of oriental medicine sludge was 5.3, which was similar to 5.2 of the main ingredient, corncob. Its sugar content, however, was 4.8 mg/g, which was 2.5 times higher than concorb's 1.9 mg/g. According to the addition content analysis of oriental medicine sludge by using blood agar plate, the experimental group showed much more robust growth than the control group. 10% of oriental medicine sludge was added to corncob and pine tree sawdust for test-tube culture. Then they were cultivated at $25^{\circ}C$ for 6 days after inoculating Flammulina velutipes liquid spawn. The control group and experimental group showed 2.2~3.4 and 5.8~6.4 cm hypae growths respectively. At the field test for 10% herbal medicine refuse, mushroom yield dropped by 5% compared to the control group. However, it had distinctively lower number of deformity and the 2nd grade products. An economic analysis was conducted based on the cultivation facility that produces 160,000 mushrooms per day. The analysis demonstrated that the facility can save 50,000,000 won in the starting year and 130,000,000 won in the following years from the unit cost of production excluding labour and operation cost.

폐 한방슬러지의 pH는 5.3으로 주재료인 콘코프의 pH 5.2와 비슷하였으나, 환원당 함량은 4.8 mg/g으로 콘코프의 1.9 mg/g 보다 2.5배 높게 나타났다. 평판배지를 사용하여 폐 한방슬러지의 첨가농도를 검토한 결과 폐 한방슬러지를 첨가하지 않은 대조구보다 5, 15 및 10%(w/w) 첨가 순으로 팽이버섯 균사 성장이 왕성하였다. 콘코프 및 미송톱밥배지에 폐 한방슬러지를 각각 10% 첨가하여 제조한 배지를 시험관에 충진시킨 다음 팽이버섯 액체종균을 접종하여 $25^{\circ}C$에서 6일 동안 배양한 결과 대조구에서는 약 2.2~3.4 cm, 시험구에서는 약 5.8~6.4 cm의 균사성장이 이루어졌다. 그리고 팽이버섯뿐만 아니라 표고버섯 및 녹각버섯(영지버섯 일종)을 접종한 시험구에서도 동일한 결과를 얻었다. 폐 한방슬러지를 10% 첨가한 배지로 현장실험을 행한 결과 팽이버섯의 수확량은 대조구보다 약 5%정도 떨어졌지만 기형버섯 발생율과 2등품 버섯의 생산량은 시험구에서 월등하게 적었다. 팽이버섯의 생산량이 16만병/1일 행하는 재배사를 기준으로 경제성 분석을 행한 결과 당해 년에는 약 5천만원이 절약되고 그 다음해부터는 인건비와 운영비를 제외한 약 1억3천만원의 생산단가 절약이 예상되었다.

Keywords

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