• Korean Journal of Microbiology
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• v.32 no.3
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• pp.192-197
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• 1994

Pseudomonas fluorescens which is able to utilize malonate as a sole carbon source was found to contain a novel 60 kb plasmid, which encodes the genes for the proteins to assimilate malonate, including malonate decarboxylase and acetyl-CoA synthetase. The evidence is as follows: The Pseudomonas cured with mitomycin C was unable to grow on malonate-medium as well as it lost plasmid. The plasmid isolated from the Pseudomonas could be introduced into E. coli strain JM103 and DH1 by transformation. The transformed E. coli was able to grow on malonate-medium and could transmit its plasmid back to the cured P. fluorescens by conjugation. The existence of the plasmid in the transformed E. coli was confirmed by hybridization with a labeled probe prepared from 12 kb segment of the plasmid. Dot hybridization showed that the copy number of the plasmid in the transformed E. coli is at least 13 times higher than in the wild type P. fluorescens. The two key enzymes, malonate decarboxylase and acetyl-CoA synthetase, were inducible by malonate in the transformed E. coli.

• Journal of the Korean Data and Information Science Society
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• v.25 no.6
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• pp.1195-1205
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• 2014

Compared with single design, powers of rank transformed statistic for testing main and interaction effects for $2{\times}2$ factorial in $4{\times}4$ latin square design are rapidly increased as effect size and replication size are increased. In general powers of rank transformed statistic are superior without regard to the diversified effect composition and the type of error distributions as nontesting factors are few and effect size are small. Powers of rank transformed statistic show much higher level than those of parametric statistic in exponential and double exponential distributions. Further powers of rank transformed statistic are very similar with those of parametric statistic in normal and uniform distributions.

• Journal of the Korean Geotechnical Society
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• v.34 no.11
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• pp.107-119
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• 2018

PHC piles, extension-plate attached PHC piles, and steel pipe attached PHC piles were installed in field test site. Axial compressive static load tests including load distribution test and Pile Driving Analyzer (after driving) were done on the tip-transformed PHC piles and the grouted tip-transformed PHC piles. Load-displacement curves of three different type of PHC piles, which are PHC pile (TP-1), extension plate attached PHC pile (TP-2) and steel pipe attached PHC pile (TP-3), showed almost the same behavior. Thus bearing capacity increase effect of the tip-transformed PHC piles was negligible. Share ratio of side resistance and end bearing resistance for PHC pile, extension plate attached PHC pile, and steel pipe attached PHC pile were 95.8% vs. 4.2%, 95.6% vs. 4.4%, and 97.8% vs. 2.2% respectively.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2001.12a
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• pp.233-236
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• 2001

In this paper, we propose a TSK-type fuzzy classifier using PCA(Principal Component Analysis), FCM(Fuzzy C-Means) clustering and hybrid GA(genetic algorithm). First, input data is transformed to reduce correlation among the data components by PCA. FCM clustering is applied to obtain a initial TSK-type fuzzy classifier. Parameter identification is performed by AGA(Adaptive Genetic Algorithm) and RLSE(Recursive Least Square Estimate). we applied the proposed method to Iris data classification problems and obtained a better performance than previous works.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1995.10a
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• pp.361-364
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• 1995

Wavelet Transform is a new tools for signal processing, such as data compressing extraction of parameter for Reconition and Diagnostics. This transform has an advandage of a good resolution compared to Fast Fourier Transform (FFT) In this study, we employ the wavelet transform for analysis of Acoustic Emission raw signal generated form rotary compressor. In abnormal condition of rotary compressor, the state of operating condition can be classified by analizing coefficient of wavelet transformed signal.

• Journal of Plant Biotechnology
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• v.34 no.3
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• pp.271-275
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• 2007

In the chloroplast transformation process, a chloroplast containing transformed chloroplast genome copies should be selected over wild-type chloroplasts on selection medium. It is more effective for a cell to become homoplasmic if the cell contains smaller number of chloroplasts. Therefore, to reduce the number of chloroplasts in mesophyll cells in tobacco, we overexpressed FtsZ to generate transgenic plants, of which mesophyll cell contained a few enlarged chloroplasts contrast to a wild-type mesophyll cell containing approximately 100 chloroplasts. It was demonstrated that transgenic leaf tissues comprising cells with a few enlarged chloroplasts gave rise to approximately 40% higher frequency of chloroplast-transformed adventitious shoots.

• Journal of Korea Multimedia Society
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• v.11 no.6
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• pp.764-776
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• 2008

Image identification is the process of checking whether the query image is the transformed version of the specific original image or not. In this paper, image identification method based on feature composition is proposed. Used features include color distance, texture information and average pixel intensity. We extract color characteristics using color distance and texture information by Modified Generalized Symmetry Transform as well as average intensity of each pixel as features. Individual feature is quantized adaptively to be used as bins of histogram. The histogram is normalized according to data type and it is used as the signature in comparing the query image with database images. In matching part, Manhattan distance is used for measuring distance between two signatures. To evaluate the performance of the proposed method, independent test and accuracy test are achieved. In independent test, 60,433 images are used to evaluate the ability of discrimination between different images. And 4,002 original images and its 29 transformed versions are used in accuracy test, which evaluate the ability that the proposed algorithm can find the original image correctly when some transforms was applied in original image. Experiment results show that the proposed identification method has good performance in accuracy test. And the proposed method is very useful in real environment because of its high accuracy and fast matching capacity.

• BMB Reports
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• v.41 no.7
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• pp.542-547
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• 2008

D-type cyclins control the onset of cell division and the response to extracellular signals during the G1 phase. In this study, we transformed a D-type cyclin gene, Nicta;CycD3;4, from Nicotiana tabacum using an Agrobacterium-mediated method. A predicted 1.1 kb cyclin gene was present in all of the transgenic plants, but not in wild-type. Northern analyses showed that the expression level of the Nicta;CycD3;4 gene in all of the transgenic plants was strong when compared to the wild-type plants, suggesting that Nicta;CycD3;4 gene driven by the CaMV 35S promoter was being overexpressed. Our results revealed that transgenic plants overexpressing Nicta;CycD3;4 had an accelerated growth rate when compared to wild-type plants, and that the transgenic plants exhibited a smaller cell size and a decreased cell population in young leaves when compared to wild-type plants.

• Korean Journal of Computational Design and Engineering
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• v.1 no.3
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• pp.224-233
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• 1996

An automatic mesh generation scheme with triangular finite elements on three-dimensional surfaces has been developed. The surface triangulation process is performed as follows. To begin, surfaces with key nodes are transformed to two-dimensional planes and the meshes with triangular elements are constructed in these planes. Finally, the constructed meshes are transformed back to the original 3D surfaces. For the mesh generation, an irregular mesh generation scheme is employed in which local mesh densities are assigned by the user along the boundaries of the analysis domain. For this purpose a looping algorithm combined with an advancing front technique using basic operators has been developed, in which the loops are recursively subdivided into subloops with the use of the best split lines and then the basic operators generate elements. Using the split lines, the original boundaries are split recursively until each loop contains a certain number of key nodes, and then using the basic operators such as type-1 and type-2, one or two triangular elements are generated at each operation. After the triangulation process has been completed for each meshing domain, the resulting meshes are finally improved by smoothing process. Sample meshes are presented to demonstrate the versatility of the algorithm.

• Korean Journal of Medicinal Crop Science
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• v.12 no.2
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• pp.123-128
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• 2004

Hyoscyamine and scopolamine are two most common tropane alkaloids found in the Solanaceae. The pEB expression vector carrying Nspmt gene was transformed to Agrobacterium rhizogenes. The growth of transgenic hairy roots were approximately to 80% of the wild type root. Transgenic hairy roots are less developed on only their branch roots than those of the wild type root. The extracts from Nspmt transgenic hairy root lines, 3 and 5 contained between 3.52 and 4.23 mg/g dry weight as hyoscyamine and did between 5.23 to 6.40 mg/g dry weight as scopolamine. These results showed that the overexpression of the pmt gene enhanced tropane alkaloids production of S. parviflora transformed roots and this improvement affected both hyoscyamine and scopolamine production.