Browse > Article
http://dx.doi.org/10.5010/JPB.2007.34.3.271

Enhancement of Chloroplast Transformation Frequency by Using Mesophyll Cells Containing a Few Enlarged Chloroplasts from Nuclear Transformed Plants in Tobacco  

Jeong, Won-Joong (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Min, Sung-Ran (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Liu, Jang-R. (Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Publication Information
Journal of Plant Biotechnology / v.34, no.3, 2007 , pp. 271-275 More about this Journal
Abstract
In the chloroplast transformation process, a chloroplast containing transformed chloroplast genome copies should be selected over wild-type chloroplasts on selection medium. It is more effective for a cell to become homoplasmic if the cell contains smaller number of chloroplasts. Therefore, to reduce the number of chloroplasts in mesophyll cells in tobacco, we overexpressed FtsZ to generate transgenic plants, of which mesophyll cell contained a few enlarged chloroplasts contrast to a wild-type mesophyll cell containing approximately 100 chloroplasts. It was demonstrated that transgenic leaf tissues comprising cells with a few enlarged chloroplasts gave rise to approximately 40% higher frequency of chloroplast-transformed adventitious shoots.
Keywords
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Guda G, Lee SB, Daniell H (2000) Stable expression of a biodegradable protein-based polymer in tobacco chloroplasts. Plant Cell Rep 19: 257-262   DOI
2 Jeong WJ, Jeong SW, Woo JW, Choi DW, Liu JR (2004) Dicistronic expression of the green fluorescent protein and antibiotic resistance genes in the plastid for tracking and selecting plastid-transformed cells in tobacco. Plant Cell Rep 22: 747-751   DOI
3 Jeong WJ, Park YI, Suh KH, Raven JA, Yoo OJ, Liu JR (2002b) A large population of small chloroplasts in tobacco leaf cells allows more effective chloroplast movement than a few enlarged chloroplasts. Plant Physiol 129: 112-121   DOI   ScienceOn
4 Zubko MK, Zubko EI, van Zuilen K, Meyer P, and Day A (2004) Stable transformation of petunia plastids. Transgenic Res 13: 523-530   DOI
5 Sikdar SR, Serino G, Chaudhuri S, Maliga P (1998) Plastid transformation in AraNdopsis thaliana. Plant Cell Rep 18: 20-24   DOI
6 De Cosa B, Moar W, Lee SB, Miller M, Daniell H (2001) Overexpression of the Bt cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystals. Nat Biotechnol 19: 71-74   DOI   ScienceOn
7 Boynton JE, Gillham NW, Harris EH, Hosler JP, Johnson NI\, Jones AR, Randolph-Anderson BL, Robertson D, Klein TM, Shark KB, and Sanford JC (1988) Chloroplast transformation in Chlamydomonas with high velocity microprojectiles. Science 240: 1534-1538   DOI
8 Daniell H (2002) Molecular strategies for gene containment in transgenic crops. Nat Biotechnol 20: 581-586   DOI
9 Daniell H, Khan MS, Allison L (2002) Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology. Trends Plant Sci 7: 84-91   DOI   ScienceOn
10 Dufourmantel N, Pelissier B, Garcon F, Peltier G, Ferullo J-M, and Tissot G. (2004) Generation of fertile transplastomic soybean. Plant Mol Biol 55: 479-489   DOI   ScienceOn
11 Svab Z, Hajdukiewicz P, Maliga P (1990) Stable transformation of plastids in higher plants. Proc Natl Acad Sci USA 87: 8526-8530
12 Svab Z, Maliga P (1993) High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene. Proc Natl Acad Sci USA 90: 913-917
13 Ruf S, Hermann M, Berger I, Carrer H, Bock R (2001) Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit. Nat Biotechnol 19: 870-875   DOI   ScienceOn
14 Kumar S, Dhingra A, and Daniell H. (2004) Stable transformation of the cotton plastid genome and maternal inheritance of transgenes. Plant Mol Biol 56: 203-216   DOI   ScienceOn
15 Lee SB, Kwon HB, Kwon SJ, Park SC, Jeong MJ, Han SE, Byun MO, Daniell H (2003) Accumulation of trehalose within transgenic chloroplasts confers drought tolerance. Mol Breeding 11: 1-13   DOI   ScienceOn
16 Okumura S, Sawada M, Park YW, Hayashi T, Shimamura M, Takase H, Tomizawa K (2006) Transformation of poplar (Populus alba) plastids and expression of foreign proteins in tree chloroplasts. Transgenic Res 15: 637- 646   DOI
17 Sidorov VA, Kasten D, Pang SZ, Hajdukiewicz PTJ, Staub JM, Nehra NS (1999) Stable chloroplast transformation in potato: use of green fluorescent protein as a plastid marker. Plant J 19: 209-216   DOI   ScienceOn
18 Staub JM, Garcia B, Graves J, Hajdukiewicz PTJ, Hunter P, Nehra N, Paradkar V, Schlittler M, Carroll JA, Spatola L, Ward D, Ye G, Rusell DA (2000) High-protein production of a human therapeutic protein in tobacco chloroplasts. Nat Biotechnol 18: 333-338   DOI   ScienceOn
19 Jeong WJ, Jeong SW, Min SR, Yoo OJ, Uu JR (2002a) Growth retardation of plants transformed by overexpression of NtFtsZ1-2 in tobacco. J Plant BioI. 45: 107-111   DOI   ScienceOn