• 제목/요약/키워드: transformation efficiency

검색결과 683건 처리시간 0.037초

New Analysis Method for Wireless Power Transfer System with Multiple n Resonators

  • Kim, Ju-Hui;Park, Byung-Chul;Lee, Jeong-Hae
    • Journal of electromagnetic engineering and science
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    • 제13권3호
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    • pp.173-177
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    • 2013
  • This paper presents a new method for analyzing the maximum efficiency of a wireless power transfer (WPT) system with multiple n resonators. The method is based on ABCD matrices and allows transformation of the WPT system with multiple n resonators into a single two-port network system. The general maximum efficiency equation of a WPT system with multiple n resonators is derived using the ABCD matrix. Use of this equation allows placement of the relay resonators for maximum efficiency even though they are asymmetrical. The general maximum efficiency equation and the method of the optimum placement are verified by a full wave simulation. The results show that the method is useful for the analysis of a WPT system with relay resonators.

Comparison of the Cell Surface Barrier and Enzymatic Modification System in Brevibacterium flavum and B. Lactofermentum

  • Jang Ki-Hyo;Britz Margaret L.
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제10권3호
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    • pp.225-229
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    • 2005
  • To investigate impediments to plasmid transformation in Brevibacterium flavum BF4 and B. lactofermentum BL1, cell surface barriers were determined by measuring growth inhibition whilst enzymatic barriers were determined by comparing DNA methylation properties. B. lactofermentum was more sensitive to growth inhibition by glycine than B. flavum. Release of cellular proteins during sonication was more rapid for B. lactofermentum than for B. flavum. Plasmid DNA (pCSL 17) isolated from B. flavum transformed recipient $McrBC^+$ strains of Escherichia coli with lower efficiency than $McrBC^-$. McrBC digestion of this DNA confirmed that B. flavum contain methylated cytidines in the target sequence of McrBc sequences but B. lactofermentum contained a different methylation pattern. DNA derived from the B. lactofermentum transformed recipient $EcoKR^+$ strains of E. coli with lower efficiency than $EcoKR^-$, indicating the presence of methylated adenosines in the target sequence of EcoK sequences. The present data describe the differences in the physical and enzymatic barriers between two species of corynebacteria and also provide some insight into the successful foreign gene expression in corynebacteria.

Transgenesis in Fish: Indian Endeavour and Achievement

  • Pandian, T.J
    • 한국양식학회지
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    • 제16권1호
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    • pp.51-58
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    • 2003
  • The first Indian transgenic fish was generated in 1991 using borrowed constructs from foreign sources. To construct transformation vectors for the indigenous fishes, growth hormone genes of rohu (r-CH), Labeo rohita and catfish, Heteropneustes fossilis were isolated, cloned and sequenced; their fidelity was confirmed in prokaryotic and eukaryotic systems. A vector was constructed with grass carp b-actin promoter driving the expression of r-GH. Rohu eggs are large. fragile and swell 2~3 times. when fertilized. Hence they were amenable only for electroporated sperm-mediated gene transfer. Accordingly, the sperm electroporation technique was standardized to ensure 25% hatchling survival and 37% Presumptive transgenics without suffering any deformity. Southern analysis confirmed genomic integration in 15% of the tested individuals (Ti) belonging to family lines 2 and 3: another 25% of the Juveniles (Te) were also proved transgenic but with the transgene persisting extrachromosomally for longer than 1 to 2 years. perhaps due to the presence of replicon in the vector. Transgenics belonging to different family lines grew 6~8 times faster than the respective controls. Difference in growth trends of Ti and Te within a family line was not significant. In the Ti family 3 remarkable growth acceleration was sustained for a period longer than 36 weeks but in those of family 2, it gradually decreased. All transgenic fishes including the rohu converted the food at a significantly higher efficiency. Barring the transgenic mudloach, all the other transgenic fishes consumed food at significantly reduced rate.

대규모 전력계통의 미소신호 안정도 해석을 위한 Hessenberg Process의 수렴특성 가속화 방법 (A Method to Accelerate Convergence of Hessenberg process for Small Signal Stability Analysis of Large Scale Power Systems)

  • 송성근;남해곤;심관식;문채주;김용구
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1998년도 하계학술대회 논문집 C
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    • pp.871-874
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    • 1998
  • It is most important in small signal stability analysis of large scale power systems to compute only the dominant eigenvalues selectively with numerical stability and efficiency. Hessenberg process is numerically very stable and identifies the largest eigenvalues in magnitude. Hence, transformed system matrix must be used with the process. Inverse transformation with complex shift provides high selectivity centered on the shift, but does not possess the desired property of computing the dominant mode first. Thus, advantage of high selectivity of the transformation can be fully utilized only when the complex shift is given close to the dominant eigenvalues. In this paper, complex shift is determined by Fourier transforming the results of dynamic simulation with PTI's PSS/E transient simulation program. The convergence in Hessenberg process is accelerated using the iterative scheme. Overall, a numerically stable and very efficient small signal stability program is obtained. The stability and efficiency of the program has been validated against New England 10-machines 39-bus system and KEPCO system.

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Characterization of Plasmids from Multiple Antibiotic Resistant Vibrio sp. Isolated from Molluscs and Crustaceans

  • Manjusha, Sayd;Sarita, Ganabhat Bhat
    • 한국미생물·생명공학회지
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    • 제40권3호
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    • pp.197-207
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    • 2012
  • This study investigated the role of plasmids and their relationship with the multiple antibiotic resistance of 30 Vibrios sp. isolated from molluscs and crustaceans sampled from the Kerala coastal waters of India. The biochemical identification and antibiotic resistance profiles were determined, followed by the plasmid profiles, conjugation and transformation efficiencies. The results showed a considerable difference in the level of bacterial resistance to various antibiotics; while all 30 strains were found to be MAR Vibrios sp. and their resistance patterns varied. All the strains were resistant to amoxycillin, ampicillin and carbeniciliin. 87% were resistant to rifampicin; 74% to cefuroxime; 67 to streptomycin; 53% to norfloxacin and ciprofloxacin and 47% to furazolidone and nalidixic acid. In addition to their antibiotic resistance, the plasmid DNA of the MAR Vibrios strains isolated from the molluscs and crustaceans was also studied. Nine strains isolated from crustaceans and molluscs were found to harbor 1-3 plasmids with sizes varying from 5. 98 kb to 19. 36 kb. The average transformation efficiency was about $5{\times}10^{-8}$ and the conjugation efficiency varied from $2.1{\times}10^{-3}$ to $10^{-9}$. A further study of antibiotic resistance patterns may be useful to test the extent of drug resistance in seafoods and help to devise a nationwide antibiotic policy.

인공지능(AI) 기반 애플리케이션 도입이 의료기관의 운영효율성을 향상시킬까?: 기회와 도전 (Does Artificial Intelligence (AI)-based Applications Improve Operational Efficiency in Healthcare Organizations?: Opportunities and Challenges)

  • 이돈희
    • 품질경영학회지
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    • 제52권3호
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    • pp.557-574
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    • 2024
  • Purpose: This study investigates whether adoption of AI-based systems and technologies improve operational efficiency in healthcare organizations through a systematic review of the literature and real-world examples. Methods: In this study, we divided the AI application cases into care services and administrative functions, then we explored opportunities and challenges in each area. Results: The analysis results indicate that the care service field primarily uses AI-based systems and technologies for quick disease diagnosis and treatment, surgery and disease prediction, and the provision of personalized healthcare services. In the administrative field, AI-based systems and technologies are used to streamline processes and automate tasks for the following functions: patient monitoring through virtual care support systems; automating patient management systems for appointment times, reservations, changes, and no-shows; facilitating patient-medical staff interaction and feedback through interaction support systems; and managing admission and discharge procedures. Conclusion: The results of this study provide valuable insights and significant implications about the application of AI-based systems or technologies for various innovation opportunities in healthcare organizations. As digital transformation accelerates across all industries, these findings provide valuable information to managers of hospitals that are interested in AI adoption, as well as for policymakers involved in the formulation of medical regulations and laws.

파랑 변형 해석을 위한 복합 요소 모형 (Hybrid Element Model for Wave Transformation Analysis)

  • 정태화;박우선;서경덕
    • 한국해안해양공학회지
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    • 제15권3호
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    • pp.159-166
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    • 2003
  • 본 연구에서는 천해 파랑 계산에 널리 사용되어지고 있는 확장형 완경사 방정식과 계산 효율은 같게 유지하면서 Laplace방정식을 직접 풀 수 있는 유한 요소 모형에 대해서 연구하였다. 기존의 확장형 완경사 방정식을 사용하는 경우와 같은 계산효율을 유지하기 위하여 파동장을 수심방향으로 1층인 유한요소로 나누고, 요소내의 포텐셜을 수면에 위치한 절점에 대한 포텐셜만으로 표시하도록 한 후, Galerkin 기법을 적용하여 수치모형을 구성하였다. 요소 내 수평방향에 대해서는 통상의 보간함수를 채택하였으며, 수심방향에 대해서는 진행파의 수심방향 거동인 함수를 사용하여 보간함수를 구성하였다. 모형의 개발은 우선 연직 2차원 문제를 대상으로 하였다. 개발된 모형의 검증을 위하여 연직 2차원에서의 파랑 반사 및 전달문제에 적용한 결과, 개발된 유한 요소 모형은 계산상의 효율면에서나 해의 정확도 면에서 기존의 확장형 완경사 방정식에 기초한 모형과 같은 수준을 보임을 확인하였다.

Autographa californica 핵다각체병 바이러스의 IE1 유전자에 의해 형질전환된 곤충세포주의 특성 (Characterization of Insect Cells Transformed with Autographa californica Nuclear Polyhedrosis Virus IE1 Gene)

  • 조은숙;박혜진;이광식;강석우;윤은영;김근영;손흥대;진병래
    • 대한바이러스학회지
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    • 제29권2호
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    • pp.137-144
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    • 1999
  • Transformation efficiency, virus multiplication and foreign gene expression were characterized in the insect cells transformed with Autographa californica nuclear polyhedrosis virus (AcNPV) immediate early 1 gene (IE1). Transformation efficiency of insect cells by AcNPV IE1 gene vector horboring foreign gene was approximately 8-fold higher in the Sf9 cells transformed previously with AcNPV IE1 gene than in the normal Sf9 cells. Virus multiplication and foreign gene expression of recombinant baculovirus in the Sf9 cells transformed with AcNPV IE1 gene were similar to those of the normal Sf9 cells. These results suggest that transformed cells displaying foreign gene product by using AcNPV IE1 gene promoter will be useful for the diverse applications of insect cells.

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Cephalosporium acremonium의 자율복제 기점의 특성 (Distinctive Characteristics of an Autonomous Replication Sequence of Cephalosporium acremoniurn in Yeast)

  • 이경;강대욱;윤병대;황인규;안종석;민태익
    • 한국미생물·생명공학회지
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    • 제19권3호
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    • pp.215-221
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    • 1991
  • YIp5를 사용하여 Cephalosporium acremonium ATCC 2033으로부터 Saccharomyces cerevisiae SHY3에서 발현되는 자율복제 기점 (ARS)를 분리하였다. 자율복제 기점을 갖는 40종의 vector 가운데 가장 안정성 (stability)이 높은 plasmid를 pCY-2라 명명하였으며 pCY-2는 C.acremonium에서 유래하는 3.7kb의 단편을 갖고 있었다. 또 Southern hybridization과 재형질전환을 통해 pCY-2가 효모내에서 자율적으로 복제되고 있다는 것을 확인하였다. 또한 pCY-2는 형질전환율과 안정성에 있어 효모의 ARS를 갖는 YRp7 vector보다 우수하였다.

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제조실행시스템의 기능 보완을 위한 웹 기반 공장 모니터링시스템의 설계 및 구현 (Design and Implementation of Web-based Factory Monitoring System for Complement MES)

  • 김윤기;강문설;김병기
    • 정보처리학회논문지D
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    • 제9D권4호
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    • pp.667-676
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    • 2002
  • 인터넷으로 대표되는 디지털 환경은 생활 전반에 걸쳐 엄청난 변화를 주고 있으며 산업체의 비즈니스 방식과 업무 수행 방식을 빠른 속도로 바꾸어 놓고 있다. 전통 산업인 제조업체에도 인터넷과 웹 관련 기술, 초고속정보통신망을 활용하여 기존의 업무 프로세스를 개선하고 생산성 향상 및 관리 효율을 극대화하고자 하는 e-전환(e-Transformation)의 추진이 활발하게 전개되고 있다. 본 논문에서는 공장의 관리 효율 제고를 목적으로 국내외에 분산된 공장들의 현재 시점 가동 현황을 통합하여 언제 어디에서나 모니터링하기 위한 웹 기반 공장 모니터링시스템을 설계하고 구현하였다. 제안된 시스템은 웹 기반의 시스템 구조와 수행 기능, 그리고 관리 데이터를 표준화시키고, UML(Unified Modeling Language)을 이용하여 설계하였으며, ASP(Active Server Pages)를 활용하여 웹 기능을 구현하였다. 구현된 웹 기반 공장 모니터링 시스템은 K 주식회사 타이어사업부의 두개 공장(Kl, K2)을 대상으로 적용하고 있으며, 적용 결과는 전체 공장의 운영 상황을 종합적으로 파악하는데 매우 효율적인 것으로 평가되었다.