Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
권호 표지

Transgenesis in Fish: Indian Endeavour and Achievement

  • Pandian, T.J (School of Biological Sciences, Madurai Kamaraj University)
  • Published : 2003.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

The first Indian transgenic fish was generated in 1991 using borrowed constructs from foreign sources. To construct transformation vectors for the indigenous fishes, growth hormone genes of rohu (r-CH), Labeo rohita and catfish, Heteropneustes fossilis were isolated, cloned and sequenced; their fidelity was confirmed in prokaryotic and eukaryotic systems. A vector was constructed with grass carp b-actin promoter driving the expression of r-GH. Rohu eggs are large. fragile and swell 2~3 times. when fertilized. Hence they were amenable only for electroporated sperm-mediated gene transfer. Accordingly, the sperm electroporation technique was standardized to ensure 25% hatchling survival and 37% Presumptive transgenics without suffering any deformity. Southern analysis confirmed genomic integration in 15% of the tested individuals (Ti) belonging to family lines 2 and 3: another 25% of the Juveniles (Te) were also proved transgenic but with the transgene persisting extrachromosomally for longer than 1 to 2 years. perhaps due to the presence of replicon in the vector. Transgenics belonging to different family lines grew 6~8 times faster than the respective controls. Difference in growth trends of Ti and Te within a family line was not significant. In the Ti family 3 remarkable growth acceleration was sustained for a period longer than 36 weeks but in those of family 2, it gradually decreased. All transgenic fishes including the rohu converted the food at a significantly higher efficiency. Barring the transgenic mudloach, all the other transgenic fishes consumed food at significantly reduced rate.

Keywords

References

  1. Int. J. Anim. Sci. v.10 Transfer of human growth hormone gene into Indian major carp (Labeo rohita) Alok, D.;Haque, N. S.;Killan, J. S.
  2. Ph. D. thesis, Madurai Kamaraj University Studies on gene cloning and manipulation in a selected fish Anathy, V.
  3. J. Biosci. v.26 Cloning, sequencing and expression of cDNA encoding growth hormone from Indian catfish (Heteropneustes fossilis) Anathy, V.;Venugopal, T.;Koteeswaran, R.;Pandian, T. J.;Mathavan, S.
  4. J. Biosci. v.26 Application of Interpro for the functional classification of the proteins of fish origin in Swiss-Prot and TrEMBL. Biswas, M.;Kanapin, A.;Apweiler, R.
  5. Mol. Biol. Biotechnol. v.1 No transgenic rainbow trout produced with sperm incubated with linear DNA Chourrout, D.;Perrot, E.
  6. Darnell, J.;Lodish, H.;Baltimore, D.
  7. Biotechnology v.10 Growth enhancement in transgenic Atlantic salmon by the use of an all-fish chimeric growth hormone gene construct Du, S. J.;Gong, Z.;Fletcher, G. L.;Shears, M. A.;King, M. J.;Idler, D. R.;Hew, C. L
  8. Trans. Amer. Fish. Soc. v.116 Transfer of metallothionein human growth hormone fusion gene into channel catfish. Dunham, R. A.;Eash, J.;Askins, J.;Towers, T. M.
  9. Nucleic Acids Res. v.18 Transient expression directed by homologous and heterologous promoter enhancer sequences in fish cells Friedenreich, H.;Schartl, M.
  10. Aquaculture v.107 Sperm cells as vectors for introducing foreign DNA into zebrafish. Khoo, H. W.;Ang, L. H.;Lim, H. B.;Wong, K. Y.
  11. Biotechnology v.8 Development of expression vectors for transgenic fish Liu, Z.;Moav, B.;Faraz, A. J.;Guise, K. S.;Kapuscienski, A. R.;Hackett, P. B.
  12. Aquaculture v.172 Transmission and expression of an integrated reporter construct in three generations of transgenic mudloach (Misgurnus mizolepis). Nam, Y.K.;Noh, C. H.;Kim, D. S.
  13. Transgenic Res. v.10 Dramatically accelerated growth and extraordinary gigantism of transgenic mudloach (Misgurnus mizolepis) Nam, Y. K.;Noh, J. K.;Cho, Y. S.;Cho, H. J.;Cho, K. N.;Kim, C. G.;Kim, D. S.
  14. Curr. Sci. v.81 Guidelines for research and utilization of genetically modified fish. Pandian, T. J.
  15. Curr. Sci. v.66 Problems and prospects of transgenic fish production. Pandian, T. J.;Marian, L. A.
  16. Curr. Sci. v.60 Microinjection of rat growth hormone gene into zebrafish eggs and production of transgenic zebrafish. Pandian, T. J.;Kavumpurath, S.;Mathavan, S.;Dharmalingam, K.
  17. Ph. D. thesis, Madurai Kamaraj University Construction of transformation vectors and genetic manipulation in selected fishes. Sheela, S. G.
  18. J. Biosci. v.23 Construction, electroporetic transfer and expression of ZpbypGH ZpbrtGH in zebrafish Sheela, S. G.;Chen, J. D.;Mathavan, S.;Pandian, T. J.
  19. Aquacult. Res. v.30 Electroporetic transfer stable integration, expression of ZpbypGH ZpbrtGH in Indian catfish Heteropneustes fossilis. Sheela, S. G.;Chen, J. D.;Mathavan, S.;Pandian, T. J.
  20. Development v.103 Replication, integration and stable germ-line transmission of foreign sequences injected into early zebrafish embryos. Stuart, G. W.;Mcmurray, J. V.;Westerfield, M.
  21. Can. J. Fish Aquat. Sci. v.52 Electroporation of sperm to introduce foreign DNA into the genome of loach (Misgurnus anguillicaudatus) Tsai, H. J.;Tseng, F. S.;Liao, I.
  22. Ph. D. thesis, Madurai Kamaraj University Cloning and characterization of growth hormone encoding cDNA of Indian major caprs for transgenesis. Venugopal, T.
  23. Curr. Sci. v.74 Gene transfer in Indian major carps by electroporation. Venugopal, T.;Pandian, T. J.;Mathavan, S.;Sarangi, N.
  24. Gen. Comp. Endocrinol. v.125 Molecular cloning of growth hormone encoding cDNA of an Indian major carp, Labeo rohita and its expression in E. coli and zebrafish Venugopal, T.;Anathy, V.;Pandian, T. J.;Mathavan, S.
  25. J. Bio-sci. v.27 Cloning and characterization of growth hormone encoding cDNA of Indian major carps (Pisces): application of a modified RACE strategy in cloning highly conserved cDNA sequences. Venugopal, T.;Mathavan, S.;Pandian, T. J.
  26. J. Exp. Zool. Growth acceleration and transgene persistence in Indian major carp, Labeo rohita. Venugopal, T.;Anathy, V.;Pandian, T. J.