• Title/Summary/Keyword: total reducing ability

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Antioxidant Activity of 11 Species in Korean Native Forest Plants (국내 산림식물 11종의 항산화 활성)

  • Cho, MyoungLae;Lee, Jong Seok;Lee, Sarah;Son, Youn Kyoung;Bae, Chang-Hwan;Yeo, Joohong;Lee, Hae-Sock;Ma, Jin-Gyeong;Lee, Ok-Hwan;Kim, Jong-Yae
    • The Korean Journal of Food And Nutrition
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    • v.28 no.6
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    • pp.1098-1106
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    • 2015
  • This study aimed to investigate antioxidant activities from 11 forest plants, and determine their total phenolics, flavonoids and proantocyanidins contents. In addition, the antioxidant activities were correlated with antioxidant compounds. Among the samples, Cornus officinalis, Castanea crenata, Lindera erythrocarpa, Carpinus laxiflora and Pourthiaea villosa showed significantly higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) ($IC_{50}=21.12{\sim}28.93{\mu}g/mL$) and 2,2'-azino-bis(3-ethylbenzothia zoline-6-sulfonic acid) (ABTS) ($IC_{50}=28.18{\sim}52.55{\mu}g/mL$) radical scavenging ability with reducing power ($IC_{50}=59.91{\sim}93.64{\mu}g/mL$) than other plants; and C. crenata, L. erythrocarpa and Rubus coreanus showed strong nitric oxide (NO) inhibition activity (${\geq}60%$). In addition, L. erythrocarpa, C. laxiflora and P. villosa showed higher oxygen radical absorbance capacity (ORAC) values (${\geq}1,100{\mu}M$ TE/g sample) than other samples. High total phenolic contents were observed in C. crenata (429.11 mg GAE/g), L. erythrocarpa (437.11 mg GAE/g), C. laxiflora (408.67 mg GAE/g) and P. villosa (404.11 mg GAE/g). The DPPH and ABTS radical scavenging activity with reducing power were significantly correlated with total phenolic contents ($R^2=0.71{\sim}0.79$), but total phenolic contents were not correlated with NO inhibition and ORAC ($R^2=0.35{\sim}0.43$). Therefore, these results suggested that C. officinalis, C. crenata, L. erythrocarpa, C. laxiflora and P. villosa are potential natural antioxidative candidate ingredients.

Optimization of the Extraction Parameters of Gardenia (Gardenia jasminoides Ellis) Fruits for the Maximum Antioxidant Capacity

  • Yang, Bin;Liu, Xuan;Teng, Dike;Gao, Yanxiang
    • Food Science and Biotechnology
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    • v.18 no.4
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    • pp.867-871
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    • 2009
  • Response surface methodology (RSM) was used for the optimization of antioxidant capacity in gardenia extracts. The antioxidant capacities of gardenia fruit extracts were investigated by ferric reducing ability (FRA) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity (RSA) assays. The optimum extraction parameters for the strongest antioxidant capacity were the ethanol concentration (EtOH) of 48.9%, extraction temperature of $72.9^{\circ}C$, and extraction time of 29.9 min. Analysis of variance (ANOVA) showed that the quadratics of EtOH and extraction temperature had highly significant effect on the antioxidant capacity (p<0.001). The antioxidant capacity was correlated with contents of bioactive components [crocin, geniposide, and total phenolic (TP) compounds] in gardenia extracts and mainly attributed to the content of the TP compounds.

A Study on Acculturation Stress among Married Immigrant Women in the Jeonbuk Area (전북지역 결혼이주여성의 문화적응 스트레스에 관한 연구)

  • Cho, In Ju
    • Korean Journal of Human Ecology
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    • v.21 no.5
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    • pp.889-901
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    • 2012
  • This study examined acculturation stress resulting from socio-demographic characteristics and psychological and social status of married immigrant women residing in the Jeonbuk region. Data were gathered from married immigrant women who were participating in non-profit agencies offering multi-cultural services and those who worked for business offices and stores in the Jeonbuk area. A structured self-administered questionnaire was used. A total of 280 questionnaires were distributed, and 210 were returned. Twenty three questionnaires were excluded from the study because of invalid responses, providing a final data pool of 187 responses. Hierarchical multiple regression analyses revealed that Korean language ability and levels of depression were significantly associated with acculturation stress among married immigrant women. Based on the findings of this study, interventions for reducing acculturation stress among married immigrant women and implications for future studies are discussed.

Antioxidant and Acetylcholinesterase Inhibition Activity of Mulberry Fruit Extracts

  • Lee, Young-Ju;Lee, Ka-Hwa;Ahn, Chang-Bum;Chun, Soon-Sil;Je, Jae-Young
    • Food Science and Biotechnology
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    • v.18 no.6
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    • pp.1532-1536
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    • 2009
  • The objective of this study was to evaluate the antioxidant effects and acetylcholinesterase (AChE) inhibition activity of mulberry fruit extracts prepared by hot water (MFH) and 80% ethanol (MFE). Total polyphenolic contents of MFH and MFE were $195{\pm}3.4\;mg$ gallic acid equivalents/g MFH and $185{\pm}2.8\;mg$ gallic acid equivalents/g MFE. MFH and MFE significantly quenched 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide dose-dependently, and showed high chelating ability and reducing power in non-cellular systems. MFH and MFE also inhibited the formation of intracellular reactive oxygen species and lipid peroxidation, and elevated intracellular glutathione (GSH) levels in RAW264.7 cells. In addition, MFH and MFE also dose-dependently suppressed AChE activity.

Scheduling and Feedback Reduction in Coordinated Networks

  • Bang, Hans Jorgen;Orten, Pal
    • Journal of Communications and Networks
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    • v.13 no.4
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    • pp.339-344
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    • 2011
  • Base station coordination has received much attention as a means to reduce the inter-cell interference in cellular networks. However, this interference reducing ability comes at the expense of increased feedback, backhaul load and computational complexity. The degree of coordination is therefore limited in practice. In this paper, we explore the trade-off between capacity and feedback load in a cellular network with coordination clusters. Our main interest lies in a scenario with multiple fading users in each cell. The results indicate that a large fraction of the total gain can be achieved by a significant reduction in feedback. We also find an approximate expression for the distribution of the instantaneous signal to interference-plus-noise ratio (SINR) and propose a new effective scheduling algorithm.

Learning ability and Stress by Character type in Nursing College Students (간호 대학생의 성격유형별 학업능력과 스트레스)

  • Cha, Nam-Hyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.12
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    • pp.6257-6264
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    • 2013
  • The aim of this study was to identify the influencing factors and the relationships between the learning ability and stress according to the character type in nursing college students. A total of 209 subjects were selected by convenient sampling. The data was collected using a self-reported questionnaire from November 2 to 20, 2012. The differences in the character type, learning ability and stress according to the general characteristics were as follows. The character type was significantly different according to grade (F=6.551, p<.036). In addition, the learning ability was significantly different according to the grade (F=7.059, p<.001), religion (F=4.651, p<.001), school record (F=7.667, p<.001), economic status (F=7.700, p<.000) and methods of reducing stress (F=2.531, p<.030). Stress was significantly different according to the grade (F=7.414, p<.001), school record (F=4.776, p<.009) and cohabitation (F=9.989, p<.000). A negative correlation was observed between the character type and learning ability, and between learning ability and stress. 8.4% of the variance was observed in character type A, which was explained by the learning ability and stress. These findings may be useful for understanding the learning ability of nursing college students and developing more specific programs regarding personality and stress control.

Evaluation of Abelmoschus Esculentus Seed for Antioxidant and Phytochemical Analysis Using In vitro Assays

  • Rahman, Md. Saifur;Yoo, Jin Cheol
    • Journal of Integrative Natural Science
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    • v.7 no.4
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    • pp.260-265
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    • 2014
  • The aim of this study was to assess the in vitro potential of methanolic seed extract of Abelmoschus esculentus as a natural antioxidant. The DPPH activity of the Ethyl acetate soluble fraction (10, 20, 40, 80, and $160{\mu}g/mL$) was increased in a dose dependent manner, which was found in the range of 18.97-90.47% as compared to ascorbic acid 26.44-93.71%. The $IC_{50}$ values of Ethyl acetate soluble fraction (EAES) and ascorbicacid in DPPH radical scavenging assay were obtained to be 28.12 and $18.43{\mu}g/mL$, respectively. Measurement of polyphenol content of the EAES of A. esculentus seed was achieved using Folin-Ciocalteau reagent containing 53.80 mg/g of total phenolic content, which was found signicantly higher when compared to reference standard gallic acid. Similarly total flavonoids and proabthocyanidis of EAES and chloroform soluble fraction (CAES) were found significantly 147 mg/g and 14.24 mg/g respectively when both compared to reference standard quercetin. EAES exhibited high significant lipid peroxidation inhibition effects in a dose-dependent manner, with $IC_{50}$ values of $38.08{\mu}g/mL$, whereas, standard quercetin, with $IC_{50}$ value of $36.67{\mu}g/mL$. All extract/fractions showed dose dependent reducing power ability and these differences were statistically significant (p<0.001). The results obtained in this study clearly indicate that A. esculentus seed has a signicant potential to use as a natural antioxidant agent.

Study of the Antioxidant Effect of Ojayeonjong-hwan (오자연종환(五子衍宗丸) 추출물의 항산화 효과에 관한 연구)

  • Jung, Yu-jin;Park, Sang-eun;Hong, Sang-hoon
    • The Journal of Internal Korean Medicine
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    • v.43 no.3
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    • pp.344-362
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    • 2022
  • Objective: In this study, the antioxidant activity of Ojayeonjong-hwan extracts was compared, and the following results were obtained. Methods: For hydrothermal and ethanol extracts, DPPH free radical and ABTS cationic radical erasing activity and reducing power using the FRAP method were compared, and the association between the antioxidant power of each extract and total phenol content was investigated. Significant results were obtained through in vitro apoptosis analysis through FFITC staining, mitochondrial membrane potential analysis, and ROS level measurement using C2C12 myoblastoma. Results: 1. In a comparison of DPPH free radical and ABTS cationic radical scavenging activity, water, and 70% ethanol extracts of Ojayeonjong-hwan (WEO and EEO) showed superior radical scavenging ability. 2. In the results of reducing power using the FRAP method, WEO and EEO showed antioxidant activity, which was shown to be dependent on the total phenol content contained in the extracts. 3. In comparison to the protective effect against H2O2-induced oxidative stress in C2C12 myoblasts, water extracts had no significant effect, but 70% ethanol extracts inhibited H2O2-mediated cytotoxicity in a concentration-dependent manner. 4. The cytotoxic protective effect of EEO against oxidative stress in C2C12 myoblasts was correlated with its inhibitory effects on H2O2-induced apoptosis and cell-cycle arrest. 5. In H2O2-treated C2C12 myoblasts, the apoptosis inhibitory effects of EEO were associated with the suppression of mitochondrial dysfunction and DNA damage. 6. The protective effects of EEO against H2O2-induced oxidative stress in C2C12 myoblasts were directly related to the inhibition of ROS generation. Conclusions: Ojayeonjong-hwan extracts all have protective potential against oxidative stress.

Antioxidant Activity of Wa-song (Orostachys japonicus A. Berger) According to Drying Methods (건조 방법에 따른 와송의 항산화 효과)

  • Lee, Soo-Jung;Seo, Jong-Kwon;Shin, Jung-Hye;Lee, Hyun-Ji;Sung, Nak-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.5
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    • pp.605-611
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    • 2008
  • Antioxidant activity of wa-song (Orostachys japonicus A. Berger) was analyzed to clarify the influence of extractive solvent and drying method such as sun, hot-air and freeze drying. The contents of total phenols and flavonoids were significantly higher in 95% ethanol extracts than water extracts. Ability of reducing power and DPPH radical, hydroxyl radical and nitrite scavenging ability were higher in the order of hot-air dried sample> freeze dried sample> sun dried sample and these abilities were also higher in 95% ethanol extracts than water extracts. In conclusion, antioxidant activities of wa-song extracts were in proportion to the contents of total phenols and flavonoids. Also, hot-air drying is the superior method for the enhancement of antioxidant activity of wa-song.

Improvement of rooster semen freezability and fertility rate after sericin supplementation in freezing semen extender

  • Ruthaiporn Ratchamak;Supakorn Authaida;Wuttigrai Boonkum;Vibuntita Chankitisakul
    • Animal Bioscience
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    • v.36 no.10
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    • pp.1530-1535
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    • 2023
  • Objective: Semen cryopreservation result in decreased sperm parameters and fertilization ability. Sericin exhibits antioxidant activity by reducing lipid peroxidation resulting from free radicals, which can potentially improve cryopreservation outcomes. The present study aimed to examine the efficacy of various sericin concentrations supplemented with a rooster semen-freezing extender on post-thaw semen quality and fertilizing ability of sperm after cryopreservation. Methods: Semen samples were collected from 40 roosters (5 reps), then were pooled, and divided into four groups by the levels of sericin supplementation (0%, 0.25%, 0.50%, and 0.75%) in a freezing extender. Semen suspensions were loaded in medium straw (0.5 mL) and cryopreserved with the traditional liquid nitrogen vapor method. Post-thawed semen was evaluated for sperm motility, sperm viability, and lipid peroxidation. Also, the fertility test was determined. Results: The results showed that supplementation of the freezing extender with 0.50% to 0.75% sericin resulted in greater total motility and progressive motility and lower malondialdehyde levels than the other groups after cryopreservation (p<0.05). However, the viability of 0.75% decreased compared with the value of 0.50% sericin supplementation (p<0.05). Moreover, the fertility and hatchability of total eggs were significantly higher in the 0.50% sericin group than in the other groups (p<0.05). Conclusion: In conclusion, 0.50% sericin is recommended as an alternative component of the freezing extender to improve cryopreserved rooster semen.