Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Improvement of rooster semen freezability and fertility rate after sericin supplementation in freezing semen extender

  • Received : 2023.01.20
  • Accepted : 2023.03.21
  • Published : 2023.10.01
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Abstract

Objective: Semen cryopreservation result in decreased sperm parameters and fertilization ability. Sericin exhibits antioxidant activity by reducing lipid peroxidation resulting from free radicals, which can potentially improve cryopreservation outcomes. The present study aimed to examine the efficacy of various sericin concentrations supplemented with a rooster semen-freezing extender on post-thaw semen quality and fertilizing ability of sperm after cryopreservation. Methods: Semen samples were collected from 40 roosters (5 reps), then were pooled, and divided into four groups by the levels of sericin supplementation (0%, 0.25%, 0.50%, and 0.75%) in a freezing extender. Semen suspensions were loaded in medium straw (0.5 mL) and cryopreserved with the traditional liquid nitrogen vapor method. Post-thawed semen was evaluated for sperm motility, sperm viability, and lipid peroxidation. Also, the fertility test was determined. Results: The results showed that supplementation of the freezing extender with 0.50% to 0.75% sericin resulted in greater total motility and progressive motility and lower malondialdehyde levels than the other groups after cryopreservation (p<0.05). However, the viability of 0.75% decreased compared with the value of 0.50% sericin supplementation (p<0.05). Moreover, the fertility and hatchability of total eggs were significantly higher in the 0.50% sericin group than in the other groups (p<0.05). Conclusion: In conclusion, 0.50% sericin is recommended as an alternative component of the freezing extender to improve cryopreserved rooster semen.

Keywords

Acknowledgement

This research was supported by the fundamental fund of Khon Kaen University and Thailand Science Research and Innovation.

References

  1. Wang AW, Zhang H, Ikemoto I, Anderson DJ, Loughlin KR. Reactive oxygen species generation by seminal cells during cryopreservation. Urology 1997;49:921-5. https://doi.org/10.1016/s0090-4295(97)00070-8
  2. Chuaychu-noo N, Thananurak P, Chankitisakul V, Vongpralub T. Supplementing rooster sperm with Cholesterol-Loaded-Cyclodextrin improves fertility after cryopreservation. Cryobiology 2017;74:8-12. https://doi.org/10.1016/j.cryobiol.2016.12.012
  3. Blesbois E, Grasseau I, Seigneurin F. Membrane fluidity and the ability of domestic bird spermatozoa to survive cryopreservation. Reproduction 2005;129:371-8. https://doi.org/10.1530/rep.1.00454
  4. Partyka A, Nizanski W. Supplementation of avian semen extenders with antioxidants to improve semen quality-is it an effective strategy? Antioxidants (Basel) 2021;10:1927. https://doi.org/10.3390/antiox10121927
  5. Khiabani AB, Moghaddam G, Kia HD. Effects of adding different levels of Glutamine to modified Beltsville extender on the survival of frozen rooster semen. Anim Reprod Sci 2017;184:172-7. https://doi.org/10.1016/j.anireprosci.2017.07.013
  6. Kato N, Sato S, Yamanaka A, Yamada H, Fuwa N, Nomura M. Silk protein, sericin, inhibits lipid peroxidation and tyrosinase activity. Biosci Biotechnol Biochem 1998;62:145-7. https://doi.org/10.1271/bbb.62.145
  7. Isobe T, Iketaba Y, Onitsuka T, et al. Effect of sericin on preimplantation development of bovine embryos cultured individually. Theriogenology 2012;78:747-52. https://doi.org/10.1016/j.theriogenology.2012.03.021
  8. Takechi T, Wada R, Fukuda T, Harada K, Takamura H. Antioxidant activities of two sericin proteins extracted from cocoon of silkworm (Bombyx mori) measured by DPPH, chemiluminescence, ORAC and ESR methods. Biomed Pep 2014;2:364-9. https://doi.org/10.3892/br.2014.244
  9. Aghaz F, Khazaei M, Vaisi-Raygani A, Bakhtiyari M. Cryoprotective effect of sericin supplementation in freezing and thawing media on the outcome of cryopreservation in human sperm. Aging Male 2020;23:469-76. https://doi.org/10.1080/13685538.2018.1529156
  10. Raza S, Ucan U, Aksoy M, Erdogan G, Ceylan A, Serin I. Corrigendum to "Silk protein sericin pretreatment enhances osmotic tolerance and post-thaw sperm quality but reduces the ability of sperm cells to undergo in vitro induced acrosome reaction in rabbit". Cryobiology 2019;91:198. https://doi.org/10.1016/j.cryobiol.2019.10.008
  11. Ratchamak R, Thanaporn R, Kheawkanha T, Vongpralub T, Boonkum W, Chankitisakul V. Evaluation of cryopreserved boar semen after supplementation sericin form silkworm (Bombyx mori) in semen extender. Anim Sci J 2020;91: e13428. https://doi.org/10.1111/asj.13428
  12. Yangngam Y, Chapanya S, Vongpralub T, Boonkum W, Chankitisakul V. Effect of semen extender supplementation with sericin on post-thaw dairy bull sperm quality and lipid peroxidation. Czech J Anim Sci 2021;66:13-20. https://doi.org/10.17221/104/2020-CJAS
  13. Chauychu-noo N, Thananurak P, Boonkum W, Vongpralub T, Chankitisakul V. Effect of organic selenium dietary supplementation on quality and fertility of cryopreserved chicken sperm. Cryobiology 2021;98:57-62. https://doi.org/10.1016/j.cryobiol.2020.12.008
  14. Schramm GP. Suitability of different antifreeze agents for cryoprotection of cock sperm. Monatsh Veterinarmed 1991;46:438-40.
  15. Chankitisakul V, Boonkum W, Kaewkanha T, et al. Fertilizing ability and survivability of rooster sperm diluted with a novel semen extender supplemented with serine for practical use on smallholder farms. Poult Sci 2022;101:102188. https://doi.org/10.1016/j.psj.2022.102188
  16. Kumar P, Kumar D, Sikka P, Singh P. Sericin supplementation improves semen freezability of buffalo bulls by minimizing oxidative stress during cryopreservation. Anim Reprod Sci 2015;152:26-31. https://doi.org/10.1016/j.anireprosci.2014.11.015
  17. Moce E, Blanch E, Tomas C, Graham JK. Use of cholesterol in sperm cryopreservation: present moment and perspectives to future. Reprod Domest Anim 2010;45:57-66. https://doi.org/10.1111/j.1439-0531.2010.01635.x
  18. Blesbois E, Grasseau I, Seigneurin F, Mignon-Grasteau S, Saint Jalme M, Mialon-Richard MM. Predictors of success of semen cryopreservation in chickens. Theriogenology 2008;69:252-61. https://doi.org/10.1016/j.theriogenology.2007.09.019
  19. Mussa NJ, Ratchamak R, Ratsiri T, et al. Lipid profile of sperm cells in Thai native and commercial roosters and its impact on cryopreserved semen quality. Trop Anim Health Prod 2021;53:321. https://doi.org/10.1007/s11250-021-02664-9
  20. Parks JE, Lynch DV. Lipid composition and thermotropic phase behavior of boar, bull, stallion, and rooster sperm membranes. Cryobiology 1992;29:255-66. https://doi.org/10.1016/0011-2240(92)90024-V
  21. Rahal A, Kumar A, Singh V, et al. Oxidative stress, prooxidants, and antioxidants: the interplay. Biomed Res Int 2014;2014:761264. https://doi.org/ 10.1155/2014/761264
  22. Bucak MN, Atessahin A, Varisli O, Yuce A, Tekin N, Akcay A. The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen: Microscopic and oxidative stress parameters after freeze-thawing process. Theriogenology 2007;67:1060-7. https://doi.org/10.1016/j.theriogenology.2006.12.004
  23. Ansari M, Zhandi M, Kohram H, Zaghari M, Sadeghi M, Sharafi M. Improvement of post-thawed sperm quality and fertility of Arian rooster by oral administration of d-aspartic acid. Theriogenology 2017;92:69-74. https://doi.org/10.1016/j.theriogenology.2017.01.014
  24. Zhandi M, Ansari M, Roknabadi P, Zare Shahneh A, Sharafi M. Orally administered chrysin improves post-thawed sperm quality and fertility of rooster. Reprod Dom Anim 2017;52:1004-10. https://doi.org/10.1111/rda.13014
  25. Chuaychu-noo N, Thananurak P, Chankitisakul V, Vongpralub T. Supplementing rooster sperm with Cholesterol-Loaded-Cyclodextrin improves fertility after cryopreservation. Cryobiology 2017;74:8-12. https://doi.org/10.1016/j.cryobiol.2016.12.012
  26. Islam MA, Nishibori M. Crossbred chicken for poultry production in the tropics. J Poult Sci 2010;47:271-9. https://doi.org/10.2141/jpsa.010033
  27. Sasanami T, Matsuzaki M, Mizushima S, Hiyama G. Sperm storage in the female reproductive tract in birds. J Reprod Dev 2013;59:334-8. https://doi.org/10.1262/jrd.2013-038
  28. Kheawkanha T, Boonkum W, Vongpralub T, Chankitisakul V. Characterization of oviduct lining, with emphasis on the sperm storage tubule region (uterovaginal junction), correlated with fertility in mature and old Thai native hens. Animals 2021;11:3446. https://doi.org/10.3390/ani11123446
  29. Peebles ED, Brake J, Gildersleeve RP. Effects of eggshell cuticle removal and incubation humidity on embryonic development and hatchability of broilers. Poult Sci 1987;66:834-40. https://doi.org/10.3382/ps.0660834
  30. Ghasemi M, Farshad A, Hajarian H, Banafshi O, Asadollahi V, Fathi F. The effects of sericin on cryopreserved sperm cells and subsequent embryo development in mice. Int J Reprod Biomed 2018;16:405-12. https://doi.org/10.29252/ijrm.16.6.405