• Journal of Pharmacopuncture
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• v.21 no.4
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• pp.268-276
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• 2018

Objectives: The purpose of this study is to investigate the anti-cancer effects of different fractions of Astragalus membranaceus (AM) in human non-small cell lung cancer (NSCLC) cells. Methods: We isolated hexane, ethyl acetate, and butanol fractions from crude ethanol extract of AM. The cell death was examined by MTT assay and trypan blue exclusion assay. Apoptosis was detected by DAPI staining, annexin V-PI double staining and cell cycle analysis. The expression of apoptosis-related proteins and mitogen-activated protein kinases (MAPKs) was examined by western blot. Results: Among various fractions of AM, the ethyl acetate fraction of AM (EAM) showed the strongest cytotoxic effect in NSCLC cells. EAM reduced the cell proliferation in a time- and dose-dependent manner in NSCLC cells. In addition, EAM induced the chromatin condensation, and increased the population of sub-G1 phase and annexin V-positive cells in a time-dependent manner, indicating that EAM induced apoptosis in NSCLC cells. Consistently, EAM enhanced the expression of cleaved caspase-8 and -9, and induced the accumulation of cleaved- poly (ADP-ribose) polymerase (PARP). Among MAPK proteins, only ERK was dephosphorylated by EAM, suggesting that ERK might be related with EAM-induced apoptosis. Conclusion: Our results clearly demonstrate that EAM exhibited anti-cancer effects in NSCLC cells by induction of apoptosis. We provide a valuable evidence which suggests that AM could be a desirable therapeutic option for treatment of NSCLC.

• Structural Engineering and Mechanics
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• v.69 no.1
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• pp.1-10
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• 2019

In the construction of flat plate slabs, which are widely used for tall buildings but have relatively low flexural stiffness, serviceability problems such as excessive deflections and cracks are of great concern. To prevent excessive deflections at service load levels, current design codes require the minimum slab thickness, but the requirement could be unconservative because it is independent on loading and elastic modulus of concrete, both of which have significant effects on slab deflections. In the present study, to investigate the effects of the construction load of shored slabs, reduced flexural stiffness and moment distribution of early-age slabs, and creep and shrinkage of concrete on immediate and time-dependent deflections, numerical analysis was performed using the previously developed numerical models. A parametric study was performed for various design and construction conditions of practical ranges, and a new minimum permissible thickness of flat plate slabs was proposed satisfying the serviceability requirement for deflection. The proposed minimum slab thickness was compared with current design code provisions and numerical analysis results, and it agreed well with the numerical analysis results.

• Steel and Composite Structures
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• v.18 no.4
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• pp.889-907
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• 2015

This paper aims to present an alternative analytical method for transient vibration analysis of doubly-curved laminated shells subjected to dynamic loads. In the method proposed, the governing differential equations of laminated shell are derived using the dynamic version of the principle of virtual displacements. The governing equations of first order shear deformation laminated shell are obtained by Navier solution procedure. Time-dependent equations are transformed to the Laplace domain and then Laplace parameter dependent equations are solved numerically. The results obtained in the Laplace domain are transformed to the time domain with the help of modified Durbin's numerical inverse Laplace transform method. Verification of the presented method is carried out by comparing the results with those obtained by Newmark method and ANSYS finite element software. Also effects of number of laminates, different material properties and shell geometries are discussed. The numerical results have proved that the presented procedure is a highly accurate and efficient solution method.

• Journal of the Korean Society for Precision Engineering
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• v.18 no.7
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• pp.127-133
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• 2001

The effects of triangular and rectangular discharge current waveforms on anode erosion in wire electric discharge machining is investigated based on heat transfer analysis of half-space subject to time and space-dependent heat flux. The thermal load on the anode is simulated by a hear flux that is calculated from time-dependent discharge channel radius and energy. Evolution of the melting front during discharge, the molten volume at the experimental result for qualitative verification of the analysis. It is demonstrated that the triangular current waveform is more efficient in eroding the anode than the rectangular one.

• Proceedings of the Korea Concrete Institute Conference
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• 1994.10a
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• pp.368-373
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• 1994

Recently, a large number of box girder bridges with cantilevered decks have been constructed. Especially, segmentally erected prestressed concrete box girder bridges are widely used as economic and aesthetic solutions for long span bridges. Segmental erection is a particularly attractive construction alternative in cases where continuously supported formwork is impractical or uneconomical. In segmentally erected bridges, the structural systems are changed as the construction stages are progressive and redistribution of member forces occurs due to time dependent effects of concrete and relaxation of prestressing steel. Then, in segmentally erected bridges, analysis are required at each construction states. In this study, nonlinear analysis progam of the segmentally erected prestressed concrete box girder bridges is developed in taking into account nonlinearity of material and geometry, time dependent effect of concrete and relaxation of prestressing steel.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 1999.04a
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• pp.210-217
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• 1999

This paper deals with the analysis for column shortening of RC frame structure considering the construction sequences. The time-dependent effects of concrete are taken into consideration in this study to simulate the actual structural behavior. The stiffness matrix of a beam element is derived on the bases of the layer approach, dividing a section with imaginary layers. Creep and shrinkage strains at each layer are calculated by using the first-order algorithm based on the expansion of creep compliance. Finally, the correlation studies with the purpose of analyzing the time-dependent behavior of building structure are conducted using the analytical model proposed in this study.

• International Journal of Oral Biology
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• v.32 no.2
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• pp.45-49
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• 2007

In this study, the cytotoxicity of commonly used local anesthetics was evaluated on odontoblasts which are essential for pulpal homeostasis in vitro. Local anesthetics, such as articaine, bupivacaine, levobupivacaine, lidocaine, mepivacaine, prilocaine, and procaine, were tested on the odontoblast cell line, MDPC-23. The concentration-and time-dependent cytotoxic effects of local anesthetics on odontoblasts were measured by MTT assay. Among local anesthetics treated for 18 h, only bupivacaine significantly showed cell death in a concentration-($LC_{50}=1.2mM$) and time-dependent manner. To confirm cell death induced by bupivacaine, the observation of cell morphology and FACS using Annexin V and propidium iodide (PI) staining were performed. As a result of Annexin V and PI staining, as well as the morphological change, only bupivacaine induced apoptotic cell death on odontoblasts when compared with levobupivacaine and lidocaine. These results suggest that bupivacaine might affect normal pulpal integrity even after uneventful local anesthesia.

• Journal of Ocean Engineering and Technology
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• v.7 no.2
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• pp.162-172
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• 1993

In the present paper, the overall state of the arts of ice mechanics which is the most typical research topic of the artic engineering field was studied. And also, ice loads genrated by ice-structure interaction were estimated using numerical approach. The effects of viscous property of ice sheets to the ice load were investigated. The time dependent deformation behaviors of ice was modeled by visco-plastic problem using the finite element formalism. Constitutive model representing the material properties of ice was idealized by comblned rheological model with Maxwell and Voigt models. Numerical calculations for the bending and crushing behavior of ice sheet which are the most typical interaction modes between ice sheets and structures were carried out. The time dependent viscous behaviors of ice sheets interaction forces acting on structures were analyzed and the results were studied in detail.

• Journal of Korea Society of Industrial Information Systems
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• v.17 no.3
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• pp.27-34
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• 2012

This paper presents the impact of time dependent dielectric breakdown (TDDB, also called as gate oxide breakdown) failure on nanoscale digital CMOS Circuits. Recently, TDDB for ultra-thin gate oxides has been considered as one of the critical reliability issues which can lead to performance degradation or logic failures in nanoscale CMOS devices. Also, leakage power in the standby mode can be increased significantly. In this paper, TDDB aging effects on large CMOS digital circuits in the 45nm technology are analyzed. Simulation results show that TDDB effect on MOSFET circuits can result in more significant increase of power consumption compared to delay increase.

• Computers and Concrete
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• v.15 no.2
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• pp.183-198
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• 2015

Chloride ingress implies a complex interaction between physical and chemical process, in which heat, moisture and chloride ions transport through concrete cover. Meanwhile, reinforced concrete structure itself undergoes evolution due to variation in temperature, relative humidity and creep effects, which can potentially change the deformation and trigger some micro-cracks in concrete. In addition, all of these process show time-dependent performance with complex interaction between structures and environments. In the present work, a time-dependent behavior of chloride transport in reinforced concrete beam subjected to flexural load is proposed based on the well-known section fiber model. The strain state varies because of stress redistribution caused by the interaction between environment and structure, mainly dominated by thermal stresses and shrinkage stress and creep. Finally, in order to clear the influence of strain state on the chloride diffusivity, experiment test were carried out and a power function used to describe this influence is proposed.