• 한국미생물·생명공학회지
• /
• 제50권1호
• /
• pp.89-94
• /
• 2022

Triclosan (TCS) is an antimicrobial agent used in various human personal care products against both gram-positive and gram-negative bacteria. The purpose of this study was to evaluate the presence of TCS-resistant bacteria in sewage water in Peshawar, Khyber Pakhtunkhwa (KPK), Pakistan, for the first time. TCS-supplemented Luria Bertani (LB) agar was used to isolate TCS-tolerant bacteria. A total of 17 TCS-resistant isolates were randomly selected from a large pool of bacteria that showed growth on TCS-supplemented LB agar. Based on gram staining and physiochemical characteristics, the isolated strains were identified as Salmonella typhi (n = 6), Escherichia coli (n = 4), Citrobacter freundii (n = 4), Proteus mirabilis (n = 1), Enterobacter cloacae (n = 1), and Pseudomonas aeruginosa (n = 1). The Triclosan mean minimum inhibitory concentrations (MICs) for the isolates of Salmonella typhi, Escherichia coli, Citrobacter freundii, Proteus mirabilis, Enterobacter cloacae, and Pseudomonas aeruginosa were 23.66 ㎍ ml^-1, 18.75 ㎍ ml^-1, 42 ㎍ ml^-1, 32 ㎍ ml^-1, 64 ㎍ ml^-1, and 128 ㎍ ml^-1, respectively. The antibiogram revealed that all isolates were resistant to penicillin G (100%) and linezolid (100%), followed by ampicillin (94%), tetracycline (76%), tazobactam (76%), sulbactam/cefoperazone (64%), polymyxin PB (58%), amikacin (29.41%), aztreonam (29.41%), imipenem (5%), and gentamicin (5%). This is the first known study regarding the isolation of TCS-tolerant bacteria from sewage water in Peshawar, KPK, Pakistan. It was concluded that all the TCS-resistant isolates were multidrug resistant (MDR) gram-negative rod-shaped bacteria, mostly belonging to the Enterobacteriaceae family.

• KSBB Journal
• /
• 제14권4호
• /
• pp.460-464
• /
• 1999

Methicillin-resistant Staphlococcus aureus(MRSA)는 다양한 항생제에 저항성을 나타내며 제 3세대 cephalosporin계가 보급된 1980년대 이후 원내 감염의 중요 원인균으로 대두되고있다. 그러나 MRSA 동정의 오류나 실수로 인한 부적절한 치료에 문제가 있으며 항생제의 오용으로 인한 다제약제 내성획득이 임상에서 심각한 문제가 되거 있다. 따라서 MRSA 감염의 적절한 치료는 이 원인 균의 신속하고 믿을 수 있는 동정을 필요로 한다.본 연구에서는 분자 생물학적인 동정법을 확립하기 위하여 항생제의 내성 기구인 페니실린 결합 단백질 2‘(PBP2')를 암호화하는 mec A 유전자를 PCR 방법으로 증폭하여 MIC test 결과와 비교하여 MRSA를 동정하는데 PCR법이 유용한지를 판명하였다.각 환자의 다종 검체로부터 S. aureus 120균주를 분리하여 oxcillin으로 MIC test를 한 결과 MRSA의 비율로는 농에서는 MRSA가 61.9%로 가장 높게 판명되었다. 120균주 중 MRSA 40균주, MSSA 40균주를 성별하여 PCR 방법으로 동정하여 MIC test와 비교 한 결과, MRSA 1균주(2.5%), MSSA는 2균주(5%)의 차이만이 MIC test와 상이성을 나타내었다. 이러한 결과로 볼 때 PCR법으로 MRSA를 동정하는 것은 유용하며 일상검사 업무로서 활용성이 높을 것으로 판단되었다.

• Natural Product Sciences
• /
• 제23권2호
• /
• pp.97-102
• /
• 2017

Fourteen compounds were isolated from the stem of Angelica polymorpha. On the basis of spectral data, these compounds were identified as isoimperatorin (1), phellopterin (2), bergapten (3), xanthyletin (4), cnidilin (5), geijerine (6), (-)-3'-acetyl hamaudol (7), 7-demethylsuberosine (8), dehydrogeijerin (9), (-)-hamaudol (10), (+)-visamminol (11), divaricatol (12), scopoletin (13), and decursidate (14), respectively. Among them, compounds 4-6, 8, 9, 13, and 14 were isolated for the first time from A. polymorpha. Dehydrogeijerin (6) and geijerin (9) were isolated for the first time from genus Angelica. All isolates tested for inhibitory activity against acetylcholinesterae. Compounds 1 to 13 showed acetylcholinesterase inhibitory activity with $IC_{50}$ values ranging from 1.4 to $37.5{\mu}M$.

• 한국식품과학회지
• /
• 제41권6호
• /
• pp.681-686
• /
• 2009

Cronobacter spp.(Enterbacter sakazakii)의 건조저항성은 다른 장내 세균들보다 강한 특성을 가지고 있는 것으로 알려져 있다. 본 연구는 국내 식품으로부터 분리한 110개의 Cronobacter균주로부터 건조내성 특성을 분석하고 여러 상대습도에서의 생존특성을 연구하고자 하였다. 국내 식품에서 분리한 Cronobacter 110균주중 8시간 금속표면 건조노출에 대하여 초기균수의 약 절반정도 생존하는 고내성그룹이 30%, 대부분이 사멸하는 저내성그룹이 약 10%를 차지하여 전체 균주 중 중간정도 이상의 내성을 가지는 균주가 90% 이상을 차지하고 있었다. 15일간 장기 건조노출에 경우도 비슷한 경향을 보여 주었다. 조제분유에서 Cronobacter 건조 노출의 경우 고내성그룹과 중간내성그룹은 30일 이후에도 5 log CFU/mL에서 3 log CFU/mL 이상의 생균수를 나타내었다. 오히려 금속 표면건조 때보다 더 생존율이 높아 조제분유에서는 더 내성이 증가함을 할 수가 있었다. 따라서 국내 식품에서 분리한 대부분의 Cronobacter도 건조에 강한 내성을 가지고 있는 것으로 보인다. 건조환경에서의 biofilm 형성능력은 고내성과 중간 내성그룹 균주는 노출초기에서부터 biofilm 생성이 이루어졌다. 그러나 저내성그룹 균주의 경우에는 biofilm의 형성이 증가하지 않다가 7일 후 biofilm 생성이 많아지는 것으로 분석되었다. 따라서 Cronobacter의 biofilm 형성능은 각 그룹간 biofilm 생성의 시간적인 차이만 존재하고 대부분의 Cronobacter는 건조스트레스에서 biofilm 형성능이 비슷한 것으로 보인다. Cronobacter는 건조금속표면의 75%의 상대습도에서 24-48시간 내에 모두 사멸하는 것으로 나타났으나 40% 상대습도에서는 초기균수의 60% 생균수를 유지하여 낮은 상대습도에 강한 저항특성을 보여주었다. 대부분의 Cronobacter는 건조에 강한 내성을 가지고 있어 조제분유등의 건조식품에서 오랫동안 살아 있을 수 있으나 습도가 높은 환경에서는 사멸이 많이 일어나는 것으로 사료된다. 그러므로 식품생산현장에서 습도를 조절하여 Cronobacter를 제어할 수 있을 것으로 보인다.

• Journal of Microbiology and Biotechnology
• /
• 제19권11호
• /
• pp.1464-1469
• /
• 2009

To achieve more accurate and rapid detection of macrolide-lincosamide-streptogramin B resistance genes, erm(A), erm(B), and erm(C), we developed a TaqMan probe-based real-time PCR (Q-PCR) method and compared it with conventional PCR (C-PCR), which is the most widely using erm gene identification method. The detection limit of Q-PCR was 5 fg of genomic DNA or 5-8 CFU of bacterial cells of Staphylococcus aureus. The utilization of Q-PCR might shorten the time to erm detection from 3-4 h to about 50 min. These data indicated that Q-PCR assay appears to be not only highly sensitive and specific, but also the most rapid diagnostic method. Therefore, the appropriate application of the Q-PCR assay will permit rapid and accurate identification of erm genes from clinical and other samples.

• Journal of Communications and Networks
• /
• 제17권3호
• /
• pp.286-295
• /
• 2015

Avionic databuses fulfill a critical function in the connection and communication of aircraft components and functions such as flight-control, navigation, and monitoring. Ethernet-based avionic databuses have become the mainstream for large aircraft owning to their advantages of full-duplex communication with high bandwidth, low latency, low packet-loss, and low cost. As a new generation aviation network communication standard, avionics full-duplex switched ethernet (AFDX) adopted concepts from the telecom standard, asynchronous transfer mode (ATM). In this technology, the switches are the key devices influencing the overall performance. This paper reviews the avionic databus with emphasis on the switch architecture classifications. Based on a comparison, analysis, and discussion of the different switch architectures, we propose a new avionic switch design based on a time-division switch fabric for high flexibility and scalability. This also merges the design concept of space-partition switch fabric to achieve reliability and predictability. The new switch architecture, called space partitioned shared memory switch (SPSMS), isolates the memory space for each output port. This can reduce the competition for resources and avoid conflicts, decrease the packet forwarding latency through the switch, and reduce the packet loss rate. A simulation of the architecture with optimized network engineering tools (OPNET) confirms the efficiency and significant performance improvement over a classic shared memory switch, in terms of overall packet latency, queuing delay, and queue size.

• Journal of Microbiology and Biotechnology
• /
• 제25권9호
• /
• pp.1537-1541
• /
• 2015

Previous detection methods for Citrobacter are considered time consuming and laborious. In this study, we have developed a rapid and accurate detection method for Citrobacter species in pork products, using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). A total of 35 Citrobacter strains were isolated from 30 pork products and identified by both MALDI-TOF MS and 16S rRNA gene sequencing approaches. All isolates were identified to the species level by the MALDI-TOF MS, while 16S rRNA gene sequencing results could not discriminate them clearly. These results confirmed that MALDITOF MS is a more accurate and rapid detection method for the identification of Citrobacter species.

• 한국지구물리탐사학회:학술대회논문집
• /
• 한국지구물리탐사학회 2007년도 특별 심포지엄 논문집
• /
• pp.63-72
• /
• 2007

Current estimates of the ice-mass balance over the Greenland and the Antarctica using retrievals of time-varying gravity from GRACE are presented. Two different GRACE gravity data, UTCSR RL01 and UTCSR RL04, are used for the estimates to examine the impact of the relative accuracy of background models in the GRACE data processing for inter-annual variations of GRACE gravity data. In addition, the ice-mass balance is appraised from the conventional GRACE data, which represents global gravity, and the filtered GRACE data, which isolates the terrestrial gravity effect from GRACE gravity data. The former estimate shows that there exists similar negative trends of ice-mass balance over the Greenland from UTCSR RL01 and UTCSR RL04 while the time series from the both GRACE data over the Antarctica differ significantly from each other, and no apparent trends are observed. The result for the Greenland from the latter calculation is similar to the former estimate. However, the latter calculation presents positive trends of ice-mass balance for the Antarctica from both GRACE data. These results imply that residual oceanic geophysical signals, particularly for ocean tides, significantly corrupt the ice-mass estimate over the Antarctica as leakage error. In addition, the spatial alias of GRACE is likely to affect the ice-mass balance because the spatial spectrum of ocean tides is not conserved via GRACE sampling, and thus ocean tides contaminate terrestrial gravity signal. To minimize the alias effect, I suggest to use the combined gravity models from GRACE, SLR and polar motion.

• Journal of Microbiology
• /
• 제44권3호
• /
• pp.344-353
• /
• 2006

Symptoms of fowl cholera including orofacial edema, swollen and edematous wattles and combs, and severe respiratory disorders were detected in domestic poultry in two broiler breeder farms: one located in Gyeong-gi Province (October, 2000) and the other in Chung-cheong-nam Province (March, 2001). Gram-negative, bipolar staining bacillus was easily found in a direct smear. The biochemical properties of isolates were examined using a standard diagnosis method, proving that they were 99.7% similar to the Pasteurella multocida (P. multocida: PM), a pathogenic and causative agent of fowl cholera (FC). As a result, an FC outbreak in domestic fowls was confirmed for the first time in Korea since 1942. Because FC was detected in broiler breeder farms for the first time in 59 years at the same time as an FC outbreak was confirmed in wild birds (October, 2000), our concern was focused on whether the PM strains that originated in wild birds were transmitted into poultry forms. The possibility was tracked down by comparing phenotypic and genetic properties between the two types of PM strains. PM strains of chicken origin showed prominent differences from the PM strains of wild bird origin in both phenotypic and genetic properties. An examination of the origin of the wild bird bacteria was conducted, but no evidence has been identified that PM strains from the wild bird were introduced into domestic poultry farms.

• Fisheries and Aquatic Sciences
• /
• 제8권2호
• /
• pp.76-82
• /
• 2005

We analyzed the paralytic shellfish poisoning (PSP) toxins of the toxic marine dinoflagellate Alexandrium tamarense collected from Dadaepo and Gaduck-do in Busan and from Sujeong-ri in Jinhae Bay, Korea, in April 2003. We also analyzed the PSP toxin of mussels (Mytilus galloprovincialis) and oysters (Crassostrea gigas) collected around Busan and Jinhae Bay. PSP toxin analyses were conducted by high performance liquid chromatography (HPLC). Fifteen cultured A. tamarense isolates contained 2.78 to 57.47 fmol/cell, with nearly identical toxin profiles: major components C2, GTX4; minor components C1, GTX1, NEO; and trace components GTX2, GTX3, STX. PSP toxin contents were 0 to $492\;\mu{g}$ STXeq/100 g in mussels and 0 to $48\;\mu{g}$ STXeq/100 g in oysters. Mussels at Gijang and Sujeong-ri contained the most PSP toxin contents ($492\;\mu{g}\;STXeq/100\;g\;and\;252\;\mu{g}\;STXeq/100\;g,\;respectively$), exceeding the quarantine level ($80\;\mu{g}$ STXeq/100 g). Their dominant toxin components were C2, C1, GTX2, and GTX3; the minor components GTX1, GTX4, GTX5, and NEO were sporadically detected. Phytoplankton contained 0.774 fmol/L seawater and 1.228 fmol/L seawater at Gijang and Sujeong-ri in April. At that time, Alexandrium cells were present in the water column at Gijang at 2,577 cells/mL and at Sujeong-ri at 6,750 cells/mL. Overall, we found the high and similar PSP toxin contents in AZexandrium isolates and mussels, and a correlation between occurrence of toxic Alexandrium cells in the water column and mussel intoxification. High densities of toxic Alexandrium cells in the water column immediately preceded shellfish intoxification at Gijang and Sujeong-ri in April.