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http://dx.doi.org/10.4014/jmb.1503.03071

Development of a Rapid and Accurate Identification Method for Citrobacter Species Isolated from Pork Products Using a Matrix-Assisted Laser-Desorption Ionization Time-of-Flight Mass Spectrometry (MALDITOF MS)  

Kwak, Hye-Lim (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University)
Han, Sun-Kyung (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University)
Park, Sunghoon (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University)
Park, Si Hong (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University)
Shim, Jae-Yong (Department of Food & Biotechnology, Hankyong National University)
Oh, Mihwa (National Institute of Animal Science, Rural Development Administration)
Ricke, Steven C. (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University)
Kim, Hae-Yeong (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.9, 2015 , pp. 1537-1541 More about this Journal
Abstract
Previous detection methods for Citrobacter are considered time consuming and laborious. In this study, we have developed a rapid and accurate detection method for Citrobacter species in pork products, using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). A total of 35 Citrobacter strains were isolated from 30 pork products and identified by both MALDI-TOF MS and 16S rRNA gene sequencing approaches. All isolates were identified to the species level by the MALDI-TOF MS, while 16S rRNA gene sequencing results could not discriminate them clearly. These results confirmed that MALDITOF MS is a more accurate and rapid detection method for the identification of Citrobacter species.
Keywords
Citrobacter species; MALDI-TOF MS; pork products; identification;
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