• 한국미생물·생명공학회지
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• 제28권3호
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• pp.129-133
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• 2000

Different actinomycete strains were isolated from natural lime caves of Ondal Chemongok Hwanseon and Yongyeon which are located at Kangwon or chungcheongbook province in Korea and were identified to the genus level. Soil sam-ples were collected at 6 sites inside and 2 sites outside of each natural lime cave, As the result the strains belonging to genus Streptomyces and rare actinomycetes were isolated at the average of 2.1 and 3.4 strains per g soil on inside cave whereas which were isolated at the 6.0 and 1.8 strains per g soil on outside cave. How-ever the generic distribution of Streptomyces and rare actinomycetes isolated from outside cave was quite dif-ferent from that of inside cave. It was shown that rare actinomycetes at natural lime caves is generally highly abundant than Streptomyces.

• 동굴
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• 제64호
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• pp.37-48
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• 2004

광천 새우젓 상인들이 1960년 초부터 토굴(온도 $13\~15^{circ}C$ 습도 $85\%$)을 이용해 젓갈을 숙성시켜 맛과 신선도를 유지하면서 새우젓 집산지로 주목받았으며, 현재 전국에 광천토굴젓갈을 공급하고 있다. 광천토굴은 1949년 유명원이 폐금광에 새우젓을 저장하면서 시작되었고, 1960년초부터 본격적으로 40여개의 토굴이 굴착되어 토굴창고업이 이루어졌다 현재 저온창고가 발달해 숙성기간만 이용되므로 토굴창고업이 쇠퇴하고 있는 상황이다. 또한 판촉의 일환으로 축제의 실시와 특화단지 조성 및 위생용기의 개량에 주력하고 있다. 민속행사로는 굴대감제가 굴을 파기 전, 안택과 10월 고사와 같은 가신제의 때, 토굴에 새우젓이 들여 왔을 때 가지며 냉장시설이 없던 시절 여름철 냉장고로 활용되었다.

• 한국도로학회논문집
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• 제18권5호
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• pp.11-19
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• 2016

PURPOSES : This study identifies the causes and the mechanism of the occurrence of underground cavities. METHODS : A case study on cave-in and a series of model tests with a small soil chamber were conducted. RESULTS : A hypothesis about the mechanism of the cave-in in road was established, and the basic influencing factors on underground cavity expansion were identified. CONCLUSIONS : It was found that the characteristics of shear strength of soil and direction of water flow had a larger influence on cavity formation and expansion than the characteristics of internal erosion. In addition, large cavities suddenly expanded when cavities were caused owing to breakage of buried sewer pipe.

• 환경생물
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• 제22권1호
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• pp.213-219
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• 2004

동굴 내 정점별 세균 군집 구조를 분석하기 위하여 PCf amplified 16S rDNA denaturing gradient gel electrophoresis(DGGE)를 적용하였다. DGGE는 동일한 분자량을 갖는 dsDNA band라고 할지라도, 각각의 염기서열 차이에 따라 전기영동 상에서 고유한 band양상을 나타낼 수 있다. eubacteria의 16S rDNA V3region을 증폭하기 위해 GC341F와 PRUN518r을 primer로 사용하여 지하수내에 미생물 군집의 다양성과 유사성을 분석하였다. DGGE band 양상을 통해 동굴내의 세균 군집 구조는 외부 환경에 비해 상대적으로 종다양성이 낮으며 동굴내 에서 특이적으로 서식하는 종이 있음을 확인하였다. 또한 유기 영양물질의 공급이 제한되어 있는 동굴에서 구아노가 주요 유기 영양물질의 공급원으로서 큰 영향을 미치고 있는 것으로 파악되었다. DGGE 상의 일부 band의 염기서열분석 결과 Pseudomonas sp. NZ060과 Pseudomonas pseudoalcaligenes, uncultured Variovorax sp., soil bacterium NS7로 동정되었다.

• 동굴
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• 제67호
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• pp.21-34
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• 2005

점말동굴은 대부분이 퇴적기원인 석회 암 동굴이다. 토양은 산악지 역, 구릉지, 산록지 등에 다양한 종류의 토양이 분포한다. 동굴의 기본 골격은 주변을 통과하는 단층곡의 일부에 형성되었다. 동굴내의 소규모 수로는 절리의 방향이나 규모에 의해 유도된다. 동굴의 형태와 미지형은 그 지역의 지질구조 및 지하수의 유동과 밀접한 관련이 있다. 동굴내의 점토는 지표로부터 유입되었을 가능성이 크다. 동굴의 성인은 구조적으로 접근하는 것이 필요하다.

• 한국지반공학회논문집
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• 제33권12호
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• pp.93-105
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• 2017

본 연구에서는 하수관 손상에 의한 지반함몰 발생 과정에서 지반의 포화도 상승에 따른 흙의 불포화 강도 저하의 영향을 파악하기 위하여, 직접 전단 실험, 모형 실험, 그리고 수치해석을 수행하였다. 직접 전단 시험 결과, 흙의 마찰각은 포화도의 영향을 크게 받지 않으나, 점착력은 포화도의 영향을 크게 받음을 알 수 있다. 포화도 상승에 따른 강도저하의 영향만을 고려하기 위하여, 물의 침투효과를 배제한 모형 실험을 실시하여 지반 함몰 현상을 재현하였다. 지반 함몰은 대변위를 동반하며, 기존 유한요소법의 적용이 어렵다. 본 연구에서는 대변위 해석 기법인 일반 보간 재료점법을 사용하여 수행한 모형실험을 수치적으로 모사하였다. 비록 경계 조건 차이, 불완전 포화, 손상부 연결관의 폐색등에 의해 함몰 시간 등에는 차이가 있었지만, 유사한 토체의 변형 거동이 모형 실험과 수치해석에서 발견되었다.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2010년도 추계 학술발표회 3차
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• pp.113-116
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• 2010

Ground cave-in is usually initiated by the formation of cavity within the ground due to soil loss. When the location of the cavity is deep in the ground, the detection of the cavity is not easy. Then it is possible that the hidden cavity expands for a long time to eventually cause sudden large-scale collapse. A case of large scale ground collapse in the old fill ground was studied and described in this paper. The underground cavity appeared to be caused by subsurface erosion deep in the ground and to expand/extend upward till it was ended by the catastrophic ground failure. It highlighted the importance of proper drainage work in a large scale land fill.

• 한국미생물·생명공학회지
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• 제24권5호
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• pp.534-539
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• 1996

Total 276 soil actinomycete strains were isolated from 46 soil samples collected at domestic natural caves; the Kosu, Chundong, and Nodong caves at Chungbook province, the Kossi cave at Kangwon province, the Sungruye cave at Kyungbook province, the Hyupjae, Ssangyong, and Manjang caves at Cheju province. All of these isolates were identified to the genus level based on morphological and physiological characteristics. As the result, 52.5% of those isolates were Streptomyces, 16.3% were Micromonospora, 22.8% were Nocardioform group, 1.1% were Actinomadura, 0.3% were Nocardiopsis, 0.3% were Streptosporangium, 0.3% were Nocardioides, 1.4% were Kineosporia, 4.7% were the others. Streptomycete strains were the most abundant, but were relatively less comparing to general distribution pattern. Nocardioform and Micromonospora strains were quite abundant, and other rare actinomycete groups were somewhat abundant comparing to general distribution pattern previously reported. Especially Nocardioform strains were highly abundant at almost of the natural caves.

• Journal of Microbiology
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• 제45권2호
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• pp.105-112
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• 2007

The objective of this study was to analyze the phylogenetic composition of bacterial community in the soil of an earth-cave (Niu Cave) using a culture-independent molecular approach. 16S rRNA genes were amplified directly from soil DNA with universally conserved and Bacteria-specific rRNA gene primers and cloned. The clone library was screened by restriction fragment length polymorphism (RFLP), and representative rRNA gene sequences were determined. A total of 115 bacterial sequence types were found in 190 analyzed clones. Phylogenetic sequence analyses revealed novel 16S rRNA gene sequence types and a high diversity of putative bacterial community. Members of these bacteria included Proteobacteria (42.6%), Acidobacteria (18.6%), Planctomycetes (9.0 %), Chloroflexi (Green nonsulfur bacteria, 7.5%), Bacteroidetes (2.1%), Gemmatimonadetes (2.7%), Nitrospirae (8.0%), Actinobacteria (High G+C Gram-positive bacteria, 6.4%) and candidate divisions (including the OP3, GN08, and SBR1093, 3.2%). Thirty-five clones were affiliated with bacteria that were related to nitrogen, sulfur, iron or manganese cycles. The comparison of the present data with the data obtained previously from caves based on 16S rRNA gene analysis revealed similarities in the bacterial community components, especially in the high abundance of Proteobacteria and Acidobacteria. Furthermore, this study provided the novel evidence for presence of Gemmatimonadetes, Nitrosomonadales, Oceanospirillales, and Rubrobacterales in a karstic hypogean environment.

• 한국제4기학회지
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• 제17권2호
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• pp.145-148
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• 2003

The Kunang cave paleolithic site is located at Tanyang [$N37^{\circ}2'$, $128^{\circ}21'E$], Chungbuk Province, which is in the Central part of the Korean peninsula. The cave is developed at 312 amsl in a karstic mountainous area. The South Han River flows across this region and other caves can also be found near the river. The site was discovered in 1986 and excavated 3 times by the Chungbuk National University Museum until now. The cave was wellpreserved from modem human activities until the first discovery. The full length of the cave is estimated to be ca. 140 m. However, a spacious part up to 11 m from the entrance has been excavated. Eight lithological units are divided over the vertical profile at a depth of 5 m. Each unit is deposited in ascending order as follow: mud layer (Unit 9), lower complex (Unit 8) which is composed of angular blocks and fragments with a muddy matrix, lower travertine layer (Unit 7； flowstone), middle complex (Unit 6; cultural layer) which is composed of fragments with a muddy matrix, middle travertine layer (Unit 5； flowstone), yellowish muddy layer (Unit 4), upper complex (Unit 3； cultural layer) which has a similar composition to Unit 8. the upper travertine layer (Unit 2； flowstone), and finally surface soil layer (Unit 1). The most abundant vestiges in the cultural layers are the animal bones. They are small fractured pieces and mostly less than 3 cm in length. About 3,800 bone pieces from 25 animal species have been collected so far, 90 percent of them belonging to young deers. Previous archaeological study of these bone pieces shows thatprehistoric people occupied the cavenot for permanent dwelling but for temporary shelter during their seasonal hunting activity. More extensive studies of these bones together with pollen analysis are in progress to reconstruct the paleoenvironment of this cave. Only a single date (12,500 BP) obtained from a U-Th measurement of the upper travertine layer was previously available. In spite of the importance of the cave stratigraphy, there was no detail chronological investigation to establish the depositional process of the cultural layers and to understand the periodic structure of the cave strata, alternating travertine floor and complex layers. We have measured five 14C age dating (38900+/-1000, 36400+/-900, 40600+/-1600, more than 51000 and 52000 14C BP) using Seoul National University 14C AMS facility, conducted systematic process of the collagen extraction from bone fragments samples. From the result, we estimate that sedimentation rate of the cave earth is constant, and that the travertine layers, Unit 2 and Unit 3, was formed during MIS 5a(ca. 80 kBP) and MIS 5c (ca. 100 kBP) respectively. The Kunang Cave site is located at Yochonli of the region of Danyang in the mid-eastern part of Korea. This region is compased of limestones in which many caves were found and the Nam-han river flows meanderingly. The excavations were carried out three times in 1986, 1988, and 1998.