• Microbiology and Biotechnology Letters
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• v.28 no.3
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• pp.129-133
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• 2000

Different actinomycete strains were isolated from natural lime caves of Ondal Chemongok Hwanseon and Yongyeon which are located at Kangwon or chungcheongbook province in Korea and were identified to the genus level. Soil sam-ples were collected at 6 sites inside and 2 sites outside of each natural lime cave, As the result the strains belonging to genus Streptomyces and rare actinomycetes were isolated at the average of 2.1 and 3.4 strains per g soil on inside cave whereas which were isolated at the 6.0 and 1.8 strains per g soil on outside cave. How-ever the generic distribution of Streptomyces and rare actinomycetes isolated from outside cave was quite dif-ferent from that of inside cave. It was shown that rare actinomycetes at natural lime caves is generally highly abundant than Streptomyces.

• Journal of the Speleological Society of Korea
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• no.64
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• pp.37-48
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• 2004

Many merchants have dealed in the salted shrimps(Saewoo -Jet) in Kwangcheon. They made the soil cave at headland on beach, so aged Saewoo-Jet in casks in soil cave with fitting temperature($13\~15^{circ}C$ and humidity($85\%$). Kwangcheon where it is gathering and scattering around the country have gained fame in its tastes and the freshness in Korea. Yu Myoun Won made the fresh starts Put it in this cave. Since 1949, the soil cave is used all the time. Ultimately this was getting warehouse business put the salted shimps since 1960. Today, this business is going down, and this warehouse is used for only the aged Period. As the alternative plan, many merchants focus on various fastivals, Saewoo-Jet special area plan, vessel improvement etc, and makes a wish to good sales. Folkway are Guldeagum-Je that they has done before digging the cave, Anteak(sods that care the family dwell in the house) and the October sacrifice have done on Gasin-Je in the soil cave. This cave has used as the refrigator when Saewoo-Jet come in the cave.

• International Journal of Highway Engineering
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• v.18 no.5
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• pp.11-19
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• 2016

PURPOSES : This study identifies the causes and the mechanism of the occurrence of underground cavities. METHODS : A case study on cave-in and a series of model tests with a small soil chamber were conducted. RESULTS : A hypothesis about the mechanism of the cave-in in road was established, and the basic influencing factors on underground cavity expansion were identified. CONCLUSIONS : It was found that the characteristics of shear strength of soil and direction of water flow had a larger influence on cavity formation and expansion than the characteristics of internal erosion. In addition, large cavities suddenly expanded when cavities were caused owing to breakage of buried sewer pipe.

• Korean Journal of Environmental Biology
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• v.22 no.1
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• pp.213-219
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• 2004

The bacterial community of water stream, soil and guano in Gossi cave was examined by using PCR amplified the 16S rDNA-denaturing gradient gel electrophoyesis (DGGE). In this study, the genetic diversity and the similarity of bacterial community between open area and non - open area toy cave tour were investigated, and the seasonable variation pattern was compared each other. DGGE is attractive technique, as it sepayate same length dsDNA according to sequence variation typical 16S rDNA genes. The diversity and similarity of bacterial community in cave was analyzed by GC341f and PRUN518r primer sets foy amplification of V3 region of eubacteria 16S rDNA. The specific DGGE band profile of the cave water gives the possibility that the specific bacterial cell can be adapting to the specific cave environment and living in the cave. The DGGE band profiles of all samples with guano were compared and analyzed by image analyzer, in which mutual band profile was compared to be and the band intensity of guano was the highest. From these result, it is thought that the guano was main nutrient source and influenced on the community structure of the cave environment where is nutritionally limited. Pseudomonas sp. NZ060, Pseudomonas pseudoalcaligenes, uncultured Variovorax sp. and soli bacterium NS7 were identified to be on some sample from analysing DNA sequence of some DGGE band.

• Journal of the Speleological Society of Korea
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• no.67
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• pp.21-34
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• 2005

Jeomal Cave consists of limestone. Soil distribution is very different from mountain area to piedmont area. The Cave developed in a part of the fault valley. The joints controls the low level stream orders in the cave. in this area the geological structure is close relate to the underground water flow. It is certain that the distribution of the clay came from the surface.

• Journal of the Korean Geotechnical Society
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• v.33 no.12
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• pp.93-105
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• 2017

This study presents direct shear tests, model tests, and numerical simulations to assess the effect of reduction of soil strength because of saturation during formation of ground cave-in caused by damaged sewer pipe lines. The direct shear test results show that the saturation affects the cohesion of soil significantly although it does not influence the friction angle of soil. To experimentally reproduce ground cave-in, the model tests were performed. As ground cave-ins were accompanied with extreme deformation, conventional finite element method has difficulty in simulating them. The present study relies on generalized interpolation material point method, which is one of meshless methods. Although there are differences between the model test and numerical simulation caused by boundary conditions, incomplete saturation, and exclusion of groundwater flow, similar ground deformation characteristics are observed both in the model test and numerical simulation.

• Proceedings of the Korean Geotechical Society Conference
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• 2010.09c
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• pp.113-116
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• 2010

Ground cave-in is usually initiated by the formation of cavity within the ground due to soil loss. When the location of the cavity is deep in the ground, the detection of the cavity is not easy. Then it is possible that the hidden cavity expands for a long time to eventually cause sudden large-scale collapse. A case of large scale ground collapse in the old fill ground was studied and described in this paper. The underground cavity appeared to be caused by subsurface erosion deep in the ground and to expand/extend upward till it was ended by the catastrophic ground failure. It highlighted the importance of proper drainage work in a large scale land fill.

• Microbiology and Biotechnology Letters
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• v.24 no.5
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• pp.534-539
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• 1996

Total 276 soil actinomycete strains were isolated from 46 soil samples collected at domestic natural caves; the Kosu, Chundong, and Nodong caves at Chungbook province, the Kossi cave at Kangwon province, the Sungruye cave at Kyungbook province, the Hyupjae, Ssangyong, and Manjang caves at Cheju province. All of these isolates were identified to the genus level based on morphological and physiological characteristics. As the result, 52.5% of those isolates were Streptomyces, 16.3% were Micromonospora, 22.8% were Nocardioform group, 1.1% were Actinomadura, 0.3% were Nocardiopsis, 0.3% were Streptosporangium, 0.3% were Nocardioides, 1.4% were Kineosporia, 4.7% were the others. Streptomycete strains were the most abundant, but were relatively less comparing to general distribution pattern. Nocardioform and Micromonospora strains were quite abundant, and other rare actinomycete groups were somewhat abundant comparing to general distribution pattern previously reported. Especially Nocardioform strains were highly abundant at almost of the natural caves.

• Journal of Microbiology
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• v.45 no.2
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• pp.105-112
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• 2007

The objective of this study was to analyze the phylogenetic composition of bacterial community in the soil of an earth-cave (Niu Cave) using a culture-independent molecular approach. 16S rRNA genes were amplified directly from soil DNA with universally conserved and Bacteria-specific rRNA gene primers and cloned. The clone library was screened by restriction fragment length polymorphism (RFLP), and representative rRNA gene sequences were determined. A total of 115 bacterial sequence types were found in 190 analyzed clones. Phylogenetic sequence analyses revealed novel 16S rRNA gene sequence types and a high diversity of putative bacterial community. Members of these bacteria included Proteobacteria (42.6%), Acidobacteria (18.6%), Planctomycetes (9.0 %), Chloroflexi (Green nonsulfur bacteria, 7.5%), Bacteroidetes (2.1%), Gemmatimonadetes (2.7%), Nitrospirae (8.0%), Actinobacteria (High G+C Gram-positive bacteria, 6.4%) and candidate divisions (including the OP3, GN08, and SBR1093, 3.2%). Thirty-five clones were affiliated with bacteria that were related to nitrogen, sulfur, iron or manganese cycles. The comparison of the present data with the data obtained previously from caves based on 16S rRNA gene analysis revealed similarities in the bacterial community components, especially in the high abundance of Proteobacteria and Acidobacteria. Furthermore, this study provided the novel evidence for presence of Gemmatimonadetes, Nitrosomonadales, Oceanospirillales, and Rubrobacterales in a karstic hypogean environment.

• The Korean Journal of Quaternary Research
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• v.17 no.2
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• pp.145-148
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• 2003

The Kunang cave paleolithic site is located at Tanyang [$N37^{\circ}2'$, $128^{\circ}21'E$], Chungbuk Province, which is in the Central part of the Korean peninsula. The cave is developed at 312 amsl in a karstic mountainous area. The South Han River flows across this region and other caves can also be found near the river. The site was discovered in 1986 and excavated 3 times by the Chungbuk National University Museum until now. The cave was wellpreserved from modem human activities until the first discovery. The full length of the cave is estimated to be ca. 140 m. However, a spacious part up to 11 m from the entrance has been excavated. Eight lithological units are divided over the vertical profile at a depth of 5 m. Each unit is deposited in ascending order as follow: mud layer (Unit 9), lower complex (Unit 8) which is composed of angular blocks and fragments with a muddy matrix, lower travertine layer (Unit 7； flowstone), middle complex (Unit 6; cultural layer) which is composed of fragments with a muddy matrix, middle travertine layer (Unit 5； flowstone), yellowish muddy layer (Unit 4), upper complex (Unit 3； cultural layer) which has a similar composition to Unit 8. the upper travertine layer (Unit 2； flowstone), and finally surface soil layer (Unit 1). The most abundant vestiges in the cultural layers are the animal bones. They are small fractured pieces and mostly less than 3 cm in length. About 3,800 bone pieces from 25 animal species have been collected so far, 90 percent of them belonging to young deers. Previous archaeological study of these bone pieces shows thatprehistoric people occupied the cavenot for permanent dwelling but for temporary shelter during their seasonal hunting activity. More extensive studies of these bones together with pollen analysis are in progress to reconstruct the paleoenvironment of this cave. Only a single date (12,500 BP) obtained from a U-Th measurement of the upper travertine layer was previously available. In spite of the importance of the cave stratigraphy, there was no detail chronological investigation to establish the depositional process of the cultural layers and to understand the periodic structure of the cave strata, alternating travertine floor and complex layers. We have measured five 14C age dating (38900+/-1000, 36400+/-900, 40600+/-1600, more than 51000 and 52000 14C BP) using Seoul National University 14C AMS facility, conducted systematic process of the collagen extraction from bone fragments samples. From the result, we estimate that sedimentation rate of the cave earth is constant, and that the travertine layers, Unit 2 and Unit 3, was formed during MIS 5a(ca. 80 kBP) and MIS 5c (ca. 100 kBP) respectively. The Kunang Cave site is located at Yochonli of the region of Danyang in the mid-eastern part of Korea. This region is compased of limestones in which many caves were found and the Nam-han river flows meanderingly. The excavations were carried out three times in 1986, 1988, and 1998.