• Title/Summary/Keyword: tdcR

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Comparison of tdcA Expression Between Escherichia coli and Salmonella enterica Serovar Typhimurium

  • Kim, Min-Jeong;Lim, Sang-Yong;Ryu, Sang-Ryeol
    • Journal of Microbiology and Biotechnology
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    • v.21 no.3
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    • pp.252-255
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    • 2011
  • Both Escherichia coli (E. coli) and Salmonella enterica serovar Typhimurium (S. Typhimurium) have a tdc operon that encodes enzymes involved in a metabolic pathway for the degradation of L-serine and L-threonine. However, S. Typhimurium does not have the tdcR gene, which is a positive regulator in E. coli. In the present study, transcriptional analysis revealed that tdcA expression in E. coli is higher under anaerobic than aerobic growth conditions, but the opposite is true in S. Typhimurium. Interestingly, a tdcR mutant strain of E. coli showed a similar expression pattern to that observed in S. Typhimurium and was also induced by anaerobic shock. These results suggest that the induction of tdcA expression by anaerobic conditions is observable when tdcA expression is low owing to the absence of TdcR.

Design of High-Precision Ring Oscillator FPGA for TDC Time Measurement (TDC 시간 측정을 위한 고정밀 Ring Oscillator FPGA 설계)

  • Jin, Kyung-Chan
    • Proceedings of the IEEK Conference
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    • 2007.07a
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    • pp.223-224
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    • 2007
  • To develop nuclear measurement system with characteristics including both re-configuration and multi-functions, we proposed a field programmable gate array (FPGA) technique to implement TDC which is more suitable for high energy Physics system. In TDC scheme, the timing resolution is more important than the count rates of channel. In order to manage pico-second resolution TDC, we used the delay components of FPGA, utilized the place and route (P&R) delay difference, and then got two ring oscillators. By setting P&R area constraints, we generated two precise ring oscillators with slightly different frequencies. Finally, we evaluated that the period difference of these two ring oscillators was about 60 pico-seconds, timing resolution of TDC.

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Comparison Among Yaw and Roll Motion Controllers for Rollover Prevention (차량 전복 방지를 위한 롤 및 요 운동 제어기의 성능 비교)

  • Yim, Seongjin
    • Journal of Institute of Control, Robotics and Systems
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    • v.20 no.7
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    • pp.701-705
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    • 2014
  • This article presents a comparison among several yaw and roll motion controllers for vehicle rollover prevention. In the previous research, yaw and roll motion controllers can be independently designed for rollover prevention. Following this idea, several yaw and roll motion controllers are designed and compared in terms of rollover prevention. For the yaw motion control, PID, LQR, SMC (Sliding Mode Control) and TDC (Time-Delay Control) are adopted. For the roll motion control, LQR, LQ SOF (Static Output Feedback) control, PID, and SMC are adopted. To compare the performance of each controller, simulation is performed on a vehicle simulation package, CarSim$^{(R)}$. From simulation, TDC and LQ SOF are the best for yaw and roll motion control, respectively.

The Observation and a Quantitative Evaluation of Viable but Non-Culturable Bacteria in Potable Groundwater Using Epifluorescence Microscopy (형광현미경을 이용한 음용 지하수내 배양불능 세균의 관찰 및 정량적 평가)

  • ;Takashi Someya
    • Korean Journal of Microbiology
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    • v.38 no.3
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    • pp.180-185
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    • 2002
  • The direct viable count (DVC) and plate count (PC) methods was used to measure the number of bacteria in potable groundwater samples collected from bottled water from the market, mineral water, and edible groundwater near the urban areas and the stock farming congested areas. As a result, the number of living bacteria by DVC was comprised 30~80% of the total direct count (TDC), whereas the number of living bacteria by PC was around l~30% of DVC. Such results show that viable but non-culturable (VBNC) bacteria exist in the potable groundwater with high percentages. On the other hand, upon measuring the value from the conventional nutrient broth (NB), $10^-2$ fold diluted nutrient broth (DNB), and R2A broth, the values from the DNB and R2A showed 2~50 times higher than the conventional NB medium. These results indicate that oligotrophic bacterial groups which can multiply in the low nutrient broth abundantly exist in the oligotrophic environment like potable groundwater.

Development and Validation of Generalized Linear Regression Models to Predict Vessel Enhancement on Coronary CT Angiography

  • Masuda, Takanori;Nakaura, Takeshi;Funama, Yoshinori;Sato, Tomoyasu;Higaki, Toru;Kiguchi, Masao;Matsumoto, Yoriaki;Yamashita, Yukari;Imada, Naoyuki;Awai, Kazuo
    • Korean Journal of Radiology
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    • v.19 no.6
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    • pp.1021-1030
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    • 2018
  • Objective: We evaluated the effect of various patient characteristics and time-density curve (TDC)-factors on the test bolus-affected vessel enhancement on coronary computed tomography angiography (CCTA). We also assessed the value of generalized linear regression models (GLMs) for predicting enhancement on CCTA. Materials and Methods: We performed univariate and multivariate regression analysis to evaluate the effect of patient characteristics and to compare contrast enhancement per gram of iodine on test bolus (${\Delta}HUTEST$) and CCTA (${\Delta}HUCCTA$). We developed GLMs to predict ${\Delta}HUCCTA$. GLMs including independent variables were validated with 6-fold cross-validation using the correlation coefficient and Bland-Altman analysis. Results: In multivariate analysis, only total body weight (TBW) and ${\Delta}HUTEST$ maintained their independent predictive value (p < 0.001). In validation analysis, the highest correlation coefficient between ${\Delta}HUCCTA$ and the prediction values was seen in the GLM (r = 0.75), followed by TDC (r = 0.69) and TBW (r = 0.62). The lowest Bland-Altman limit of agreement was observed with GLM-3 (mean difference, $-0.0{\pm}5.1$ Hounsfield units/grams of iodine [HU/gI]; 95% confidence interval [CI], -10.1, 10.1), followed by ${\Delta}HUCCTA$ ($-0.0{\pm}5.9HU/gI$; 95% CI, -11.9, 11.9) and TBW ($1.1{\pm}6.2HU/gI$; 95% CI, -11.2, 13.4). Conclusion: We demonstrated that the patient's TBW and ${\Delta}HUTEST$ significantly affected contrast enhancement on CCTA images and that the combined use of clinical information and test bolus results is useful for predicting aortic enhancement.

Earthquake performance investigation of R/C residential buildings in Turkey

  • Korkmaz, Kasim Armagan;Demir, Fuat;Yenice, Tugce
    • Computers and Concrete
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    • v.15 no.6
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    • pp.921-933
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    • 2015
  • The aim of this study is to determine the earthquake performances of reinforced concrete (R/C) residential buildings in Turkey and to analyze the parameters that affect the performance. The performance of Turkish residential buildings, determined by their levels of damage, directly relates to their structural systems. Damage parameters observed from previous earthquakes define structural parameters selected to be used in the present study. Five different types of frame R/C buildings were modeled. For the analysis, the model buildings vary according to the number of stories, column sizes, and reinforcement and concrete strength parameters. The analyses consider gravity forces and earthquake loads through 1975 and 2007 Turkish design codes. In a total of 720 different R/C buildings were investigated for the analysis to obtain capacity curves. A performance evaluation was employed by considering the Turkish design code (TDC-2007). The current study ignores irregularities such as soft stories or short columns. The study's analysis considers a comparison of the parameters' influence on the structural performance of the model buildings.

Selection of Biogenic Amine-reducing Microorganisms from a Traditional Korean-style Fermented Food, $Cheonggukjang$ (전통 발효 청국장으로부터 biogenic amine 저생성 미생물의 선발)

  • Choi, Jae-Young;Hong, Sung-Wook;Chung, Kun-Sub
    • Korean Journal of Food Science and Technology
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    • v.44 no.2
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    • pp.196-201
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    • 2012
  • Microorganisms, having the lower decarboxylase activity, among the isolated strains from $cheonggukjang$ and rice-straw in this study were selected by using biogenic amine (BA) media. The selected strains were identified as $Bacillus$ $subtilis$ HH12, $B.$ $subtilis$ HR254, and $Paenibacillus$ $barcinonensis$ KR97, by using 16S rRNA analysis. PCR analysis showed that the histidine decarboxylase ($hdc$) gene was absent in the HH12, HR254, and KR97 strains. However, PCR analysis showed that the tyrosine decarboxylase ($tdc$) gene was present in the HH12, HR254, and KR97 strains. Quantitative analysis of the selected strains by using high-performance liquid chromatography showed that histamine was absent in the HH12, HR254, and KR97 strains. However, these 3 strains showed tyramine concentrations of 6.09, 3.68, and 6.30 mg/L, respectively. These strains produced lower concentrations of amines (approximately 7.9, 0, and 9.3% amines in the HH12, HR254, and KR97 strains, respectively) than the $B.$ $subtilis$ MC138 strain, which showed the higher protease activity.

Flashover Voltage of 800kV Class Composite Hollow Insulator for $SF_6$ Gas Insulated Switchgear (GIS) (800kV $SF_6$ 가스절연 개폐장치용 (GIS) 복합절연애자 (Composite Hollow Insulator)의 표면오손시 내전압특성)

  • Lee, C.R.;Yoon, J.H.;Choi, B.H.;Yoon, C.Y.;Ko, K.S.;Ban, S.G.;Huh, J.C.
    • Proceedings of the KIEE Conference
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    • 2002.07c
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    • pp.1449-1451
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    • 2002
  • A developed 800kV class composite hollow insulator for $SF_6$ Gas Insulated Switchgear (GIS) was tested as to insulation with their surfaces contaminated. The wet power frequency withstand voltage of the silicone rubber housing was about 135kV at an equivalent salt deposite density of $5kg/m^2$, to sufficiently satisfy the required performance. According to IEC 60507, the salt fog withstand voltage was measured at 565kV, and the highest measured leakage current was below 0.2mA. Therefore, it was clarified that the composite hollow insulator has sufficient insulation performance even when it is contaminated on the surface.

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An Experimental Study on the Cylinder Wall Temperature Characteristics for Load Variations in a Gasoline Engine (가솔린엔진의 부하(負荷)에 따른 실린더 벽면 온도특성(溫度特性)에 관(關)한 연구(硏究))

  • Kwon, K.R.;Ko, J.K.;Hong, S.C.
    • Journal of Power System Engineering
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    • v.3 no.1
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    • pp.16-22
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    • 1999
  • The purpose of this study is to prevent the stick, scuffing, scratch between piston and cylinder, is to contribute the piston design such as piston profile, clearance by calculating reaction force by over-lap of piston skirt, as measuring the temperature distributions of cylinder wall. The experiment has been peformed to obtain data during actual engine operation. Temperature gradient in peripheral and axial distributions of cylinder wall according to torque and speed of engine were measured by use of an 800cc class gasoline engine. The results obtained are summarized as follows ; 1) The temperature of cylinder wall at TDC was about $50{\sim}75^{\circ}C$ higher than temperature of cooling water. 2) The rear side temperature of top dead center was $141^{\circ}C$(1/4 load) in axial distribution, whereas the rear side of midway position temperature was $98^{\circ}C$. 3) The temperature of cylinder wall increased in according to rising temperature of cooling water. 4) The thrust side temperature of cylinder wall was about $15^{\circ}C$ in all load test. 5) The rear side temperature of top dead center was $159^{\circ}C$ (1/2 load) in peripheral distribution, it was about $39^{\circ}C$ higher than thrust side temperature.

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Analysis of Total Bacteria, Enteric Members of γ-proteobacteria and Microbial Communities in Seawater as Indirect Indicators for Quantifying Biofouling

  • Lee, Jin-Wook;Kim, Sung-Min;Jung, Ji-Yeon;Oh, Byung-Soo;Kim, In S.;Hong, Soon-Kang
    • Environmental Engineering Research
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    • v.14 no.1
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    • pp.19-25
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    • 2009
  • In this study, total bacteria, enteric members of the $\gamma$-proteobacteria, and microbial communities in seawater were analyzed as indirect indicators for quantifying biofouling. Biomass in seawater can significantly affect feed water pretreatment and membrane biofouling of reverse osmosis desalination processes. The purpose of this paper is to investigate microbiological quantity and quality of seawater at the potential intake of a desalination plant. For this analysis, the total direct cell count (TDC) using 4'-6-diamidino-2-phenylindole (DAPI)-staining and DNA-based real-time PCR were used to quantify the total bacteria and relative content of enteric members of $\gamma$-proteobacteria in seawater, respectively. In addition, microbial communities were examined using 16S rRNA gene cloning and bacterial isolation to identify the most abundant bacteria for a further biofouling study. The experimental results of this study identified about $10^6$ cells/mL of (total) bacteria, $10^5$ 16S rRNA gene copies/mL of enteric $\gamma$-proteobacteria, and the presence of more than 20 groups of bacteria.