• 제목/요약/키워드: tdcR

검색결과 12건 처리시간 0.03초

Comparison of tdcA Expression Between Escherichia coli and Salmonella enterica Serovar Typhimurium

  • Kim, Min-Jeong;Lim, Sang-Yong;Ryu, Sang-Ryeol
    • Journal of Microbiology and Biotechnology
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    • 제21권3호
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    • pp.252-255
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    • 2011
  • Both Escherichia coli (E. coli) and Salmonella enterica serovar Typhimurium (S. Typhimurium) have a tdc operon that encodes enzymes involved in a metabolic pathway for the degradation of L-serine and L-threonine. However, S. Typhimurium does not have the tdcR gene, which is a positive regulator in E. coli. In the present study, transcriptional analysis revealed that tdcA expression in E. coli is higher under anaerobic than aerobic growth conditions, but the opposite is true in S. Typhimurium. Interestingly, a tdcR mutant strain of E. coli showed a similar expression pattern to that observed in S. Typhimurium and was also induced by anaerobic shock. These results suggest that the induction of tdcA expression by anaerobic conditions is observable when tdcA expression is low owing to the absence of TdcR.

TDC 시간 측정을 위한 고정밀 Ring Oscillator FPGA 설계 (Design of High-Precision Ring Oscillator FPGA for TDC Time Measurement)

  • 진경찬
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2007년도 하계종합학술대회 논문집
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    • pp.223-224
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    • 2007
  • To develop nuclear measurement system with characteristics including both re-configuration and multi-functions, we proposed a field programmable gate array (FPGA) technique to implement TDC which is more suitable for high energy Physics system. In TDC scheme, the timing resolution is more important than the count rates of channel. In order to manage pico-second resolution TDC, we used the delay components of FPGA, utilized the place and route (P&R) delay difference, and then got two ring oscillators. By setting P&R area constraints, we generated two precise ring oscillators with slightly different frequencies. Finally, we evaluated that the period difference of these two ring oscillators was about 60 pico-seconds, timing resolution of TDC.

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차량 전복 방지를 위한 롤 및 요 운동 제어기의 성능 비교 (Comparison Among Yaw and Roll Motion Controllers for Rollover Prevention)

  • 임성진
    • 제어로봇시스템학회논문지
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    • 제20권7호
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    • pp.701-705
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    • 2014
  • This article presents a comparison among several yaw and roll motion controllers for vehicle rollover prevention. In the previous research, yaw and roll motion controllers can be independently designed for rollover prevention. Following this idea, several yaw and roll motion controllers are designed and compared in terms of rollover prevention. For the yaw motion control, PID, LQR, SMC (Sliding Mode Control) and TDC (Time-Delay Control) are adopted. For the roll motion control, LQR, LQ SOF (Static Output Feedback) control, PID, and SMC are adopted. To compare the performance of each controller, simulation is performed on a vehicle simulation package, CarSim$^{(R)}$. From simulation, TDC and LQ SOF are the best for yaw and roll motion control, respectively.

형광현미경을 이용한 음용 지하수내 배양불능 세균의 관찰 및 정량적 평가 (The Observation and a Quantitative Evaluation of Viable but Non-Culturable Bacteria in Potable Groundwater Using Epifluorescence Microscopy)

  • 김인기;;황경숙
    • 미생물학회지
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    • 제38권3호
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    • pp.180-185
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    • 2002
  • 직접생균수측정법(direct viable count; DVC)과 평판법(p1ate count; PC)을 이용하여 시판되고 있는 먹는샘물, 약수, 도시 인근 지역과 축산농가 밀집지역의 음용 지하수로부터 채수된 시료에 대하여 정략적 평가를 실시하였다. DVC법에 의한 생균수는 전균수(total direct count: TDC)의 약 30~80%, 평판법에 의한 생균수는 DVC의 약1~30%로 나타났다. 이와 같은 결과는 지하수내에 배양 불가능한(viable but non-culturable: VBNC)세균이 높은 비율로 존재함이라고 판단된다. 한편, 통상농도의 영양배지(nutrient broth; NB)와 이를 $10^-2 -2/배로 희석한 영양배지(diluted nutrient broth; DNB)및 R2A배지를 이용하여 계수한 결과, 통상농도의 NB배지에 비해 저영양배지인 DNB와 R2A에서 2~50배 이상 높은 계수치를 나타내었다. 이와 같은 결과로부터 지하수와 같이 빈영양한 환경 내에는 통상농도의 NB배지에서는 중식이 현저히 저해되고 저영양배지에서 중식 가능한 저영양세균이 다수 분포해 있음이라 판단되었다.

Development and Validation of Generalized Linear Regression Models to Predict Vessel Enhancement on Coronary CT Angiography

  • Masuda, Takanori;Nakaura, Takeshi;Funama, Yoshinori;Sato, Tomoyasu;Higaki, Toru;Kiguchi, Masao;Matsumoto, Yoriaki;Yamashita, Yukari;Imada, Naoyuki;Awai, Kazuo
    • Korean Journal of Radiology
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    • 제19권6호
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    • pp.1021-1030
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    • 2018
  • Objective: We evaluated the effect of various patient characteristics and time-density curve (TDC)-factors on the test bolus-affected vessel enhancement on coronary computed tomography angiography (CCTA). We also assessed the value of generalized linear regression models (GLMs) for predicting enhancement on CCTA. Materials and Methods: We performed univariate and multivariate regression analysis to evaluate the effect of patient characteristics and to compare contrast enhancement per gram of iodine on test bolus (${\Delta}HUTEST$) and CCTA (${\Delta}HUCCTA$). We developed GLMs to predict ${\Delta}HUCCTA$. GLMs including independent variables were validated with 6-fold cross-validation using the correlation coefficient and Bland-Altman analysis. Results: In multivariate analysis, only total body weight (TBW) and ${\Delta}HUTEST$ maintained their independent predictive value (p < 0.001). In validation analysis, the highest correlation coefficient between ${\Delta}HUCCTA$ and the prediction values was seen in the GLM (r = 0.75), followed by TDC (r = 0.69) and TBW (r = 0.62). The lowest Bland-Altman limit of agreement was observed with GLM-3 (mean difference, $-0.0{\pm}5.1$ Hounsfield units/grams of iodine [HU/gI]; 95% confidence interval [CI], -10.1, 10.1), followed by ${\Delta}HUCCTA$ ($-0.0{\pm}5.9HU/gI$; 95% CI, -11.9, 11.9) and TBW ($1.1{\pm}6.2HU/gI$; 95% CI, -11.2, 13.4). Conclusion: We demonstrated that the patient's TBW and ${\Delta}HUTEST$ significantly affected contrast enhancement on CCTA images and that the combined use of clinical information and test bolus results is useful for predicting aortic enhancement.

Earthquake performance investigation of R/C residential buildings in Turkey

  • Korkmaz, Kasim Armagan;Demir, Fuat;Yenice, Tugce
    • Computers and Concrete
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    • 제15권6호
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    • pp.921-933
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    • 2015
  • The aim of this study is to determine the earthquake performances of reinforced concrete (R/C) residential buildings in Turkey and to analyze the parameters that affect the performance. The performance of Turkish residential buildings, determined by their levels of damage, directly relates to their structural systems. Damage parameters observed from previous earthquakes define structural parameters selected to be used in the present study. Five different types of frame R/C buildings were modeled. For the analysis, the model buildings vary according to the number of stories, column sizes, and reinforcement and concrete strength parameters. The analyses consider gravity forces and earthquake loads through 1975 and 2007 Turkish design codes. In a total of 720 different R/C buildings were investigated for the analysis to obtain capacity curves. A performance evaluation was employed by considering the Turkish design code (TDC-2007). The current study ignores irregularities such as soft stories or short columns. The study's analysis considers a comparison of the parameters' influence on the structural performance of the model buildings.

전통 발효 청국장으로부터 biogenic amine 저생성 미생물의 선발 (Selection of Biogenic Amine-reducing Microorganisms from a Traditional Korean-style Fermented Food, $Cheonggukjang$)

  • 최재영;홍성욱;정건섭
    • 한국식품과학회지
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    • 제44권2호
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    • pp.196-201
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    • 2012
  • 본 연구에서는 청국장 및 볏짚에서 분리한 미생물중 decarboxylase 배지에서 탈탄산효소 활성이 낮은 균주로 $B.$ $subtilis$ HH12, $B.$ $subtilis$ HR254, $P.$ $barcinonensis$ KR97을 선발하였다. 이들 선발 미생물들의 histidine decarboxylase($hdc$) gene 과 tyrosine decarboxylase($tdc$) gene을 조사한 결과, $hdc$ gene은 HH12, HR254, KR97 균주에서는 검출되지 않았으나, $tdc$ gene은 HH12, HR254, KR110 균주에서 검출되었다. 또한 HPLC를 통해 선발 균주들의 배양상등액 중 amines 생성량을 분석한 결과, HH12, HR254, KR110 균주는 histamine이 검출되지 않았고 tyramine은 HH12, HR254, KR110 균주는 각각 6.09, 3.68, 6.30 mg/L로 검출되었다. 선발한 biogenic amines 저감화 균주들의 BAs 생성량을 decarboxylase 고생산 미생물로 사료되는 $B.$ $subtilis$ MC138 균주와 비교해보면 현저히 낮은 수준으로 나타났다.

800kV $SF_6$ 가스절연 개폐장치용 (GIS) 복합절연애자 (Composite Hollow Insulator)의 표면오손시 내전압특성 (Flashover Voltage of 800kV Class Composite Hollow Insulator for $SF_6$ Gas Insulated Switchgear (GIS))

  • 이창용;윤자홍;최병화;윤치영;고경석;반석걸;허종철
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2002년도 하계학술대회 논문집 C
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    • pp.1449-1451
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    • 2002
  • A developed 800kV class composite hollow insulator for $SF_6$ Gas Insulated Switchgear (GIS) was tested as to insulation with their surfaces contaminated. The wet power frequency withstand voltage of the silicone rubber housing was about 135kV at an equivalent salt deposite density of $5kg/m^2$, to sufficiently satisfy the required performance. According to IEC 60507, the salt fog withstand voltage was measured at 565kV, and the highest measured leakage current was below 0.2mA. Therefore, it was clarified that the composite hollow insulator has sufficient insulation performance even when it is contaminated on the surface.

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가솔린엔진의 부하(負荷)에 따른 실린더 벽면 온도특성(溫度特性)에 관(關)한 연구(硏究) (An Experimental Study on the Cylinder Wall Temperature Characteristics for Load Variations in a Gasoline Engine)

  • 권기린;고장권;홍성찬
    • 동력기계공학회지
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    • 제3권1호
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    • pp.16-22
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    • 1999
  • The purpose of this study is to prevent the stick, scuffing, scratch between piston and cylinder, is to contribute the piston design such as piston profile, clearance by calculating reaction force by over-lap of piston skirt, as measuring the temperature distributions of cylinder wall. The experiment has been peformed to obtain data during actual engine operation. Temperature gradient in peripheral and axial distributions of cylinder wall according to torque and speed of engine were measured by use of an 800cc class gasoline engine. The results obtained are summarized as follows ; 1) The temperature of cylinder wall at TDC was about $50{\sim}75^{\circ}C$ higher than temperature of cooling water. 2) The rear side temperature of top dead center was $141^{\circ}C$(1/4 load) in axial distribution, whereas the rear side of midway position temperature was $98^{\circ}C$. 3) The temperature of cylinder wall increased in according to rising temperature of cooling water. 4) The thrust side temperature of cylinder wall was about $15^{\circ}C$ in all load test. 5) The rear side temperature of top dead center was $159^{\circ}C$ (1/2 load) in peripheral distribution, it was about $39^{\circ}C$ higher than thrust side temperature.

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Analysis of Total Bacteria, Enteric Members of γ-proteobacteria and Microbial Communities in Seawater as Indirect Indicators for Quantifying Biofouling

  • Lee, Jin-Wook;Kim, Sung-Min;Jung, Ji-Yeon;Oh, Byung-Soo;Kim, In S.;Hong, Soon-Kang
    • Environmental Engineering Research
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    • 제14권1호
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    • pp.19-25
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    • 2009
  • In this study, total bacteria, enteric members of the $\gamma$-proteobacteria, and microbial communities in seawater were analyzed as indirect indicators for quantifying biofouling. Biomass in seawater can significantly affect feed water pretreatment and membrane biofouling of reverse osmosis desalination processes. The purpose of this paper is to investigate microbiological quantity and quality of seawater at the potential intake of a desalination plant. For this analysis, the total direct cell count (TDC) using 4'-6-diamidino-2-phenylindole (DAPI)-staining and DNA-based real-time PCR were used to quantify the total bacteria and relative content of enteric members of $\gamma$-proteobacteria in seawater, respectively. In addition, microbial communities were examined using 16S rRNA gene cloning and bacterial isolation to identify the most abundant bacteria for a further biofouling study. The experimental results of this study identified about $10^6$ cells/mL of (total) bacteria, $10^5$ 16S rRNA gene copies/mL of enteric $\gamma$-proteobacteria, and the presence of more than 20 groups of bacteria.