• 한국축산식품학회지
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• 제29권3호
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• pp.334-339
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• 2009

양념육은 돼지고기 등심을 이용하여 올리브유처리 열풍 건조 토마토 분말(OPTP) 1.5%(T1) 및 3.0%(T2) 투입과 정제 lycopene을 0.03%(T3)을 첨가하여 제품을 제조하였으며, 저장 3, 6, 9일차에 이들의 물리화학적, 미생물학적 및 관능적인 특성에 대해 조사하였다. 저장기간 동안 처리구가 대조구보다 낮은 pH값을 나타내었다. 염도는 T3를 제외하고는 저장기간이 경과함에 따라 유의적으로 증가하였다. TBARS는 저장 3일차에 OPTP가 증가할수록 낮은 TBARS값을 나타내었으나 T3는 대조구와 유의적인 차이가 나타나지 않았다. VBN함량은 저장 3일차에 T2가 가장 낮았고, 저장 9일 이후 T1 및 T2가 가장 높게 나타났다. 총균수는 저장 3일차에 T3이 가장 낮게 나타났으나 저장 6 및 9일차에는 유의적인 차이가 없었다. 명도는 저장 3일차에 T2및 T3가 대조구보다 낮게 나타났으며, 저장 기간 동안 적색도, 황색도 및 chroma 값은 처리구가 대조구보다 높은 경향이었다. 관능검사시 육색, 연도, 다즙성 및 전체적인 기호도는 시험구간 유의적인 차이가 나타나지 않았지만 향기의 경우 저장 3 및 6일차에 T2가 다른 시험구보다 유의적으로 높게 나타났다.

• 한국축산식품학회지
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• 제30권3호
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• pp.427-434
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• 2010

돼지고기 저지방 부위를 구성하고 있는 근육들 중 지방함량이 높은 5개 근육들의 냉장유통 중 품질변화를 예측하고자 냉장상태에서 14일 동안 저장하면서 근육별 이화학적 품질변화를 조사하였다. 육색소(oxy-myoglobin, metmyoglobin, total-myoglobin) 함량은 저장기간에 따른 차이가 없었다(p>0.05). 냉장저장 중 구이용으로 가능한 근육들의 저장성에서 POV 값은 모든 근육들에서 냉장저장 14일간 유의적인 변화가 없었지만(p>0.05), TBARS 값은 모든 근육들은 냉장저장 기간이 경과할수록 유의적으로 증가하였다(p<0.05). 유리지방산은 longissimus dorsi 근육이 냉장저장 기간이 경과함에 따라 유의적으로 높아졌다(p<0.05). 지방산 조성에서 냉장저장 14일까지는 모든 근육들에서 포화지방산, 불포화지방산, 단일불포화지방산, 다중불포화지방산에 유의적인 변화가 없었다(p>0.05).

• 한국식품영양과학회지
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• 제26권4호
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• pp.689-696
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• 1997

황백피와 두릅나무(AP)의 혼합 추출물이 당뇨에 미치는 영향을 알아보기 위한 실험으로 streptozotocin으로 당뇨가 유도된 생쥐에게 혼합 추출물을 5일간과 10일간 복강투여하여 다음과 같은 결과를 얻었다. 50, 150, 250mg/kg B.W. AP추출물 투여군(DI1, DI2, DI3)의 몸무게는 당뇨 대조군(DIC)에 비해 유의적 (p<0.05)인 증가를 보였으며 , 간과 췌장의 무게는 DIC군에서 증가하였으나 AP추출물의 투여로 감소되었다. 특히, 10일 복강투여군에서 장기무게의 감소정도는 5일 복강투여 군에 비해 현저하였다. 혈장 포도당수준의 변화는 AP 추출물의 투여로 유의적 (p<0.05)으로 감소하였으며 감소정도는 AP추출물의 양에 비례하는 수준이었다. 혈장 cholesterol(total, LDL, VLDL)과 TG수준은 AP추출물의 투여로 감소되었으나(p<0.05), 생쥐에는 choles-teryl ester transfer protein이 없어 HDL cholesterol의 수준은 AP추출물의 투여 후에도 변화를 보이지 않았다. TBARS 함량은 당뇨 대조군이 정상군에 비해 증가하였으나 AP추출물의 투여 후 유의적 (p<0.05)으로 감소하였다. 이러한 감소정도는 10일 복강투여군에서 현저하게 나타났으며 특히 췌장조직에서 효율적인 감소 효과를 보였다. GPx의 활성도는 DIC군에서 유의적 (p< 0.05)으로 증가하였으며 AP추출물의 투여로 감소되었다. GR의 활성도는 유의적이지 않으나 GPx와 같은 양상을 보였다. 이상의 결과에서 황백피와 두릅나무추출물은 streptozotocin으로 당뇨를 유도한 생쥐에서 몸무게의 증가, 혈당 및 혈중 지질수준의 개선, 항산화 효과를 지니고 있음이 추정되었으며, 차후 이들AP추출물의 subfraction을 통한 계속적인 연구로 혈당강하 효과의 확인 및 효과 성분의 분리에까지 연구범위를 넓힐 수 있기를 기대한다.

• Fisheries and Aquatic Sciences
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• 제4권2호
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• pp.88-92
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• 2001

To examine effects of cadmium on the respiratory burst of kidney phagocytes and antioxidant defense in liver, juvenile red seabream Pagrus major were fed a cadmium-incorporated diet $(1g\;CdC1_2/kg\;diet)$. The respiratory burst activity measured by chemiluminescence (CL) was significantly reduced by oral intake of cadmium. Lipid peroxidation in liver expressed as thiobarbituric acid reactive substances (TBARS) was significantly higher in the fish fed a cadmium-incorporated diet than that of the fish fed a control diet both on Day 3 and Day 9. Liver Glutathione S-transferase (GST) activitiy was significantly increased both on Day 3 and Day 9 by feeding a cadmium-incorporated diet, when compared with the controls. From the present results, it can be concluded that oral intake of cadmium in red seabream is associated with marked reduction of respiratory burst capacity of kidney phagocytes which can elevate susceptibility of fish against infecting pathogens. Cadmium administration also elicits significant increment of lipid peroxidation in liver, and fish try to detoxify cadmium by increasing GST activity.

• Fisheries and Aquatic Sciences
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• 제5권1호
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• pp.28-31
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• 2002

Effects of cimetidine, a cytochrome P450 inhibitor, on the benzo[a]pyrene (BaP)-mediated cytochrome P450 induction and lipid peroxidation of liver in olive flounder, Paralichthys olivaceus, were investigated. Fish were fed either a cimetidine-supplemented diet or a cimetidine-free control diet once daily to satiation for 3 days. After 6 hrs of last feeding, the fish received intraperitoneal (i.p.) injection of BaP (20 mg/kg of body weight) dissolved in sterile corn oil $(100 \mu L)$ or received only a corn oil i.p. injection. At 1, 2, 3, and 7 days after the injection, hepatic cytochrome P450 and thiobarbituric acid reactive substances (TBARS), an indicator of lipid peroxidation, were analyzed. BaP injection resulted in an increase of hepatic cytochrome P450, and the fish fed the cimetidine-supplemented diet before injection of BaP showed delayed increase of hepatic cytochrome P450 compared to the fish fed a cimetidine-free diet and BaP injected. Injection of BaP clearly induced hepatic lipid peroxidation, and consistently higher TBAR values were shown in the fish fed a cimetidine­supplemented diet before injection of BaP than the fish injected with BaP alone.

• 한국축산식품학회지
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• 제34권5호
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• pp.638-646
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• 2014

The effects of two levels (1.4 vs 2.8%) of fresh garlic on lipid oxidation and microbial growth in pork patties were evaluated. Hunter color (L, a, b), pH, thiobarbituric acid reactive substances (TBARS), oxidative volatile compounds, total bacteria and Enterobacteriaceae in the pork patties with or without fresh garlic were measured during storage at $4^{\circ}C$. Addition of fresh garlic decreased redness (a), while increased pH and yellowness (b) values of the fresh pork patties were observed, regardless of the levels added. The TBARS values of the pork patties were increased with the addition of fresh garlic (p<0.05). Similar results were observed in oxidative volatile compounds. A total of 13 volatile compounds were detected in the patties (5 sulfur-containing compounds, including allyl mercaptan, allyl methyl sulfide, diallyl sulfide, methyl-(E)-propenyl-disulfide, and diallyl disulfide, and the 8 other oxidative compounds, including 1-pentanol, hexanal, 1-hexanol, heptanal, (E)-2-heptenal, 1-octen-3-ol, (E)-2-octenal and nonanal). Fresh garlic accelerated development of oxidative products in the pork patties, especially hexanal and the total oxidative volatile compounds. However, the addition of 1.4 and 2.8% of fresh garlic inhibited the growth of total bacteria and Enterobacteriaceae, indicating low total bacterial counts and Enterobacteriaceae than the controls.

• Asian-Australasian Journal of Animal Sciences
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• 제21권7호
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• pp.1067-1072
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• 2008

Effects of fucoxanthin (FX), a major carotenoid in algae, on lipid peroxidation and meat colour in ground chicken breast meat were investigated. FX and/or ${\alpha}$-tocopherol (Toc) were added to ground samples at a content level of 200 mg/kg. The samples were subjected to a chilling storage test before and after cooking. In the storage test before cooking, neither FX nor Toc affected the thiobarbituric acid reactive substances (TBARS) on days 1 and 6, and FX decreased the L* value and increased the a* and b* values. In the storage test after cooking, both FX and Toc decreased TBARS values on days 1 and 6. FX decreased the L* value and increased the a* and b* values, similar to what occurred in the storage test before cooking. Based on these results, we concluded that FX is a potent ingredient for improvement of the appearance and shelf life of chicken meat and its products.

• 생명과학회지
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• 제11권4호
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• pp.328-334
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• 2001

Male Sprageu-Dawely rats fed a cholesterol diet (Control group) or cholesterol diet supplemented with the water-soluble extract of stem bark from Cudrania tricuspidata(CTSB group) at the level of 0.5% for 2 weeks. Concentration of triglyceride in serum of CTSB group was significantly lower than that of control group. However, the other lipids concentrations of liver and serum, body weight gain, and food intake did not show significant difference between CTSB group and control group. Antioxidative activities of water-soluble extract from stem bark of Cudrania tricuspidata on the lipid peroxidation in rat were also studied in vivo by measuring the formation of thiobarbituric acid reactive substance(TBARS). Concentration of TBATS in the liver homogenates of CTSB group was significantly higher than that in control group. Nonheme iron concentration was significantly increased in the liver of CTSB group compared to control group. suggested that enhanced nonheme irom was associated with enhanced peroxidation of liver in CTSB group. These results suggested that water soluble extract from stem bark of Cudrania tricuspidata exert the hypotriglycerolemic effect and might amplify the lipid peroxidation of tissues in cholesterol fed rats.

• 대한본초학회지
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• 제26권2호
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• pp.25-30
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• 2011

Objectives : This study was carried out to investigate an antioxidant ability, the change of antioxidant ability, antimicrobial activity and anticancer in functional meat ball and sausage prepared from Lentinus edoes mycelium and texturized soy protein. Methods : This experiments was carried out to investigate antioxidant ability(TBARS, DPPH, SOD-like ability), antibacterial activity and anticancer ability using sarcoma 180 extracts from Lentinus edoes mycelium. Result : Anticancer ability of Lentinus edoes mycelium showed a 28% survival rate and 63% of inhibition rate of tumor, which showed $1.30{\pm}0.4g$ of tumor weight. These results revealed an effective Lentinus edoes mycelium resources as anticancer sources. After heating peoducts prepared from Lentinus edoes mycelium, these products doesn't showed difference between after heating and before heating in measuring of SOD-like activity and DPPH. In DPPH experiment, ethanol extracts showed a high DPPH value as 90.85%, but hot water extracts showed 82.14% in DPPH value. Conclusion : In conclusion, it is very useful resources for preparing functional food on the basis of results from antioxidant(TBARS, DPPH, SOD-like ability), antibacterial activity and anticancer ability using sarcoma 180.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.145-148
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• 2003

DHA의 산화를 방지하기 위하여 vitamin C/alginate 리포솜에 병합시켰다. TEM을 통하여 제조된 AVDLs를 확인할 수 있었고, TLC를 통하여 각각의 AVDLs는 초기 첨가량의 15 ${\sim}$ 18 % DHA를 함유하고 있음을 확인하였다. TBARS assay에 의해$40^{\circ}C$에서 AVDLs에 포함된 DHA의 산화정도를 분석한 결과 vitamin C를 포함한 리포솜이 DHA의 산화를 억제하는 것으로 확인되었으며 0.2 % vitamin C를 포함한 AVDLs가 항산화 효과가 가장 큼을 알 수 있었다. AVDLs는 실험에서와 같은 산화조건에서 DHA의 산화를 초기 3일까지는 억제시킴을 알 수있었다.