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http://dx.doi.org/10.6116/kjh.2011.26.2.025

Development of Resources for Functional Food and Biological Activity of Lentinus edoes mycelium  

Kim, Soo-Min (Department of Herbal Foodceutical Science, Daegu Haany University)
Kim, Eun-Ju (Department of Herbal Foodceutical Science, Daegu Haany University)
Publication Information
The Korea Journal of Herbology / v.26, no.2, 2011 , pp. 25-30 More about this Journal
Abstract
Objectives : This study was carried out to investigate an antioxidant ability, the change of antioxidant ability, antimicrobial activity and anticancer in functional meat ball and sausage prepared from Lentinus edoes mycelium and texturized soy protein. Methods : This experiments was carried out to investigate antioxidant ability(TBARS, DPPH, SOD-like ability), antibacterial activity and anticancer ability using sarcoma 180 extracts from Lentinus edoes mycelium. Result : Anticancer ability of Lentinus edoes mycelium showed a 28% survival rate and 63% of inhibition rate of tumor, which showed $1.30{\pm}0.4g$ of tumor weight. These results revealed an effective Lentinus edoes mycelium resources as anticancer sources. After heating peoducts prepared from Lentinus edoes mycelium, these products doesn't showed difference between after heating and before heating in measuring of SOD-like activity and DPPH. In DPPH experiment, ethanol extracts showed a high DPPH value as 90.85%, but hot water extracts showed 82.14% in DPPH value. Conclusion : In conclusion, it is very useful resources for preparing functional food on the basis of results from antioxidant(TBARS, DPPH, SOD-like ability), antibacterial activity and anticancer ability using sarcoma 180.
Keywords
Biological activity; Antioxidant ability; Antimicrobial activity; Lentinus edoes mycelium;
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  • Reference
1 Kang YH, Park YK, Oh SR, Moon KD. Studies on the physiological functionality of pine needle and mugwort extracts. Korean J Food Sci Technol. 1995;27:978-984.
2 Pryor WA. Oxy-radicals and related species:their formation, lifetimes, and reactions. Annu Rev Physiol. 1986;48:657-667.   DOI   ScienceOn
3 Radiation and aging:free radical damage, biological response and possible antioxidant intervention. Med Hypotheses. 1993;41:473-482.   DOI   ScienceOn
4 Nice DJ, Robinson DS, Holden MA. Characterisation of a heat stable antioxidant co-purified with the superoxide dismutase activity from dried peas. Food Chem. 1995;52:393-397.   DOI   ScienceOn
5 Kang YH, Park YK, Lee GD. The nitrite scavenging and electron donating ability of phenolic compounds. Korean J Food Sci Technol. 1996;28:232-238.
6 Fukushima M, T Ohashi, Y Fujisara, K Sonoyama, M Nakano. Cholesterol-lowering effects of maitake(Grifola frondosa) fiber, and shiitake(Lentinus dodes) fiber, and enokitake (Flammulina velutipes) fiber in rats. Experimental Biology and Medicine. 2001;226:758-765.   DOI
7 Kim MW, Park MH, Kim GH. Effects of mushroom protein-bound polysaccharides on blood levels and energy metabilism in steptozotocininduced diabetic rats. Kor. J. Nutrition. 1997;30:743-750.
8 Buege JA, Aust SD. Microsomal lipid peroxidation, Method in Enzymol. 1978;105:302-310.
9 Blois MS. Antioxidant determination by the use of a stable free radical. Nature. 1958;26:1192-1198.
10 Marklund S, Marklund G. Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974;17:472-468.
11 Duncan DB. Multiple range and multiple test. Biometeics. 1955;11:1-42.   DOI   ScienceOn
12 Cojocaru IM, Cojocaru M, Musuroi C, Botezat M, Lazar L, Druta A. Lipid peroxidation and catalase in diabetes mellitus with and without ischemic stroke. Rom J Intern Med. 2004;42:423-429.
13 Saito M, Sakagami H, Fujisawa S. Cytotoxicity and apoptosis induction by butylated hydroxyanisole(BHA) and butylated hydroxytoluene (BHT). Anticancer Res. 2003;23:4693-701.
14 Takehara, M. Antiviral activity of virus-like particles from Lentinus edodes(Shiitake). Arch Virol. 1979;59:269-280.   DOI   ScienceOn
15 Lee JY. Mycology, mushroom cultivation. Seoul, Korea:Co. Deakwang. 1991:259.
16 Sung JM, Yoo YB, Cha DY. Mushroom. Seoul, Korea:Co. Kyohak. 1998:64.
17 Chihara, G. Immune modulation agents and their mechanisms(Lentinan, a T-cell oriented immunoprotentiator). NY and Basel. 1985;19:409-436.
18 Takehara, M. Antiviral activity of virus-like particles from Lentinus edodes(Shiitake). on Ehrlich ascites carcinoma in mice. Arch Virol. 1981;68:297-303.   DOI
19 Tsunoda, A. A mushroom extract as an interferon inducer. Ann NY Acad. Sci. 1969;173:719-725.
20 Bohn, JA, JN Be Miller. Beta-1,3-D-glucans as biological response modifiers. Carbohydr. Polymers. 1995;28:1433-1439.
21 Lucas EH, Ringle RU, Clarke DA, Reilly HC, Stevens JA, Stock CC. Tumor inhibitors in Boletus sdulis and other Holobasidiomycetes. Antibiot Cheotherapy. 1957;7:1-4.
22 Chihara G, Hamuro J, Maeda YY, Arai A, Fukuoka, F. Fractionation and purification of the poly-saccharides with marked antitumor activity, especially lentinan, from Lentinus edoeds (Berk.) Sins.(an edible mushroom). Res, Cancer. 1970;30:2776-2781.
23 Fujii T, Maeda H, Suzuki F, Ishida N. Isolation and characterization of a new antitumor polysaccharide, KS-2, extracted hrom culture mycelia of Lentinus edodes L. Antibiotics. 1978;31:1079-1085.   DOI
24 Bobek P, Odzin L, Kuniak L. Effect of oyster mushroom and isolated B-glucan on lipid peroxidation and on the activities of antioxidative enzymes in rats fed the cholesterol diet. Nutritional Biochem. 1997;8:469-471.   DOI   ScienceOn
25 Cheung PCK. The hypocholesterolemic effect of extracellular polysacchride from the submerged fermentation of mushroom. Nutr. Res. 1996;16:1953-1957.   DOI   ScienceOn