• 생명과학회지
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• 제21권5호
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• pp.693-699
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• 2011

Spermidine은 spermine의 전구체로 역할을 하는 putrescine로부터 합성되는 polycation의 한 형태이다. 최근에 spermidine는 수명연장에 중요한 역할을 하는 하나의 polyamine으로 알려져 있다. Hydroxyl radical, superoxide 및 hydrogen peroxide와 같은 활성산소(ROS)는 노화뿐만 아니라 다양한 병원성 과정에서 관여한다고 보고되고 있다. DPPH radical, $H_2O_2$ 및 hydroxyl radical에 대한 spermidine의 소거활성과 산화적 스트레스와 관련된 DNA oxidation에 대한 보호 효과가 in-vitro에서 평가되었다. Spermidine은 500 ${\mu}M$ 이상에서 DPPH radical와 $H_2O_2$에 대한 소거 효과를 보여주었다. 특히 활성산소종 중에서 spermidine는 hydroxyl radical에 대한 효과가 탁월하였다. 뿐만 아니라, spermidine는 500 ${\mu}M$에서 DNA oxidation에 대해서도 뚜렷한 보호 효과를 나타내었다. 더욱이 사람피부섬유아세포에서 superoxide dismutase와 같은 항산화 관련 단백질의 발현이 대조군과 비교시 spermidine 존재 하에 증가하였다. 이상의 결과로부터 spermidine은 암, 노화 및 염증을 포함하는 활성산소와 관련 있는 질병들을 예방하기 위한 항산화제로 이용될 수 있을 것이다.

• Preventive Nutrition and Food Science
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• 제13권4호
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• pp.259-265
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• 2008

This study was designed to investigate the protective effect of fucoidan against AAPH-induced oxidative stress in LLC-PK1 cells (porcine kidney epithelial cells). Oxidative stress was induced by exposing of LLC-PK1 cells to the 1 mM 2,2'-azobis(2-amidino propane) dihydrochloride (AAPH) for 24 hr. Exposure of LLC-PK1 cells to 1 mM AAPH for 24 hr resulted in a significant (p<0.05) decrease in cell viability, but fucoidan treatment protected LLC-PK1 cells from AAPH-induced cell damage in a dose dependant manner. To investigate the protective action of fucoidan against AAPH-induced damage of LLC-PK1 cells, we measured the effects of fucoidan on lipid peroxidation and antioxidant enzymes activities of AAPH treated cells as well as scavenging activities on superoxide anion radical and hydroxyl radical. Fucoidan had protective effect against the AAPH-induced LLC-PK1 cellular damage and decreased lipid peroxidation and increased activities of antioxidant enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-px). Furthermore, fucoidan showed strong scavenging activity against superoxide anion radical. The $IC_{50}$ value of fucoidan was $48.37{\pm}1.54\;{\mu}g/mL$ for superoxide anion radical scavenging activity. The fucoidan also had high hydroxyl radical scavenging activity ($IC_{50}=32.03\;{\mu}g/mL$). These results indicate that fucoidan protects against AAPH-induced LLC-PK1 cell damage by inhibiting lipid peroxidation, increasing antioxidant enzyme activities and scavenging offree radicals.

• 동의생리병리학회지
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• 제21권5호
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• pp.1135-1141
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• 2007

In recent year, We are concerned in anti-aging, disease-protection, long-life, many method are used in solving this problem. Recently, We heard that Luffae Fructus Retinervus(LFR) has effect of anti-aging, disease-protection, long-life. So I let made a experiment for this result. The purpose of this study is to; 1) the anti-oxidant effect of Luffae Fructus Retinervus(LFR) used for 3 methods, those are DPPH radical scavenging activity, Nitric oxide(NO) radical scavenging activity, Superoxide anion radical scavenging activity, 2) cultivation 3T3-L 1 Preadipocytes and Protein chip used for ProteoPlexTM 16-Well Murine Cytokine Array Kit. We measured level of DPPH radical scavenging activity. And we experienced that the ability of DPPH radical's elimination was increased by rising concentration of LFR. When the concentration of LFR was 5 mg/ml, the ability of DPPH radical's elimination was Maximum. We measured level of Nitric oxide(NO) radical scavenging activity. And we founded that the ability of NO radical's elimination was significant when concentration of LFR was from 1.25 mg/ml to 2.5 mg/ml. We measured level of Superoxide anion radical scavenging activity. And we founded that the ability of Superoxide anion radical's elimination was maximum when concentration of LFR was 0.3125 mg. When we inspected Antioxidative Effects with BSA, we experienced that ability of defense was increased by rising concentration of LFR. We known the immunity of LFR about 3T3-L1 Preadipocytes and gained the increase of Cytokines(IL-2, IL-4, GM-CSF) without IL-12p70, $INF-{\gamma}$, $TNF-{\alpha}$ So I guess that Luffae Fructus Retinervus(LFR) has effects of anti-aging, disease-protection, long-life, etc.

• Journal of Life Science
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• 제10권1호
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• pp.45-47
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• 2000

Nonactin, known as an ionophore antibiotic, was antagonized by the actibity of quercetin, an oxygen radical scavenger. This compound generated superoxide radicals in Bacillus subtilis lysates. A recombination-deficient mutant strain of B. subtilis was more sensitive than a wild strain, and this hypersensitivity was reduced in the presence of dithiothreitol as an antioxidant. These results suggest that superoxide radical is important in the antibacterial action of this agent.

• Natural Product Sciences
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• 제17권2호
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• pp.142-146
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• 2011

In the course of screening for antioxidant compounds by measuring the radical scavenging effect on 1,1-diphenyl- 2-picrylhydrazyl (DPPH), a total extract from the whole plant of Veronica peregrina (Scrophulariaceae) was found to show potent antioxidant activity. Subsequent activity-guided fractionation of the methanolic extract led to the isolation of six phenolic compounds including chrysoeriol (1), diosmetin (2), 4-hydroxybenzoic acid (3), apigenin (4), caffeic acid methylester (5) and protocatechuic acid (6). Their structures were elucidated by spectroscopic studies. Compounds 1-5 were isolated for the first time from this plant. Compounds 5 and 6 showed significant antioxidative effects in DPPH free radical scavenging and superoxide quenching activity assays.

• Archives of Pharmacal Research
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• 제27권6호
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• pp.610-614
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• 2004

The in vitro antioxidant properties of some flavone-6(4)-carboxaldehyde oxime ether deriva-tives (Ia-f, lIa-f） were determined by their effects on the rat liver microsomal NADPH-dependent lipid peroxidation (LP） levels by measuring the formation of 2-thiobarbituric acid reactive substances. The free radical scavenging properties of the compounds were also examined in vitro by determining their capacity to scavenge superoxide anions and interact with the stable free radical 2, 2-diphenyl-1-picrylhydrazyl (DPPH). The most active compounds, lib (Flavone-4＇-carboxaldehyde-O-ethyl oxime） and Id (Flavone-6-carboxaldehyde-O-［2-(1-pyrolidino） ethyl］ oxime）, caused 98 and 79％ inhibition of superoxide anion production and DPPH stable free radical at $10^{-3}{\;}M$, respectively.

• Preventive Nutrition and Food Science
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• 제14권4호
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• pp.277-282
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• 2009

The antioxidant potency of methanolic extract of Duchesnea indica (MDI; Indian strawberry) was investigated by employing various established in vitro systems, such as total phenolic content, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, reducing power assay, metal chelating assay, superoxide radical scavenging activity and protective ability of DNA damage and protein oxidation. MDI inhibited metal chelating by 75.57% at 2 mg/mL, scavenged 50% DPPH free radical at 29.13 ${\mu}$g/mL, and eliminated approximately 46.21% superoxide radical at the concentration of 1 mg/mL. In addition, MDI showed strong ability on reducing power, DNA damage protection and protein oxidation protection. Overall, results suggested that MDI might be beneficial as a potent antioxidant and effectively employed as an ingredient in food applications.

• Bulletin of the Korean Chemical Society
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• 제14권1호
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• pp.107-109
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• 1993

Non-enzymatic glycosylation and fragmentation of collagen molecule were investigated by irradiating Ultraviolet A(UV-A) with or without scavengers of reactive oxygen species (ROS) in the presence of glucose. Non-enzymatic glycosylation was increased by UV-A at high concentration of glucose. It was reduced in the presence of the scavengers of superoxide radical and singlet oxygen, but not reduced in the presence of hydroxy radical scavenger. Fragmentation of collagen was increased by UV-A, but it was decreased in the presence of all ROS scavengers tested. Superoxide radical and singlet oxygen produced by autoxidation of glucose without UV-A may encounter the initial phase of glycosylation. Data presented here suggest that UV-A affects only on the fragmentation process, but all ROS except hydroxy radical act on both processes. It appears that hydroxy radical does not act on the glycosylation process.

• 대한화장품학회지
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• 제35권3호
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• pp.251-256
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• 2009

개가시나무 가지의 에탄올 추출물에서 항산화활성을 관찰하였으며, 활성성분을 규명하기 위한 연구를 진행하였다. 그 결과, 4종류의 화합물을 분리하여 동정하였다. 분리 동정된 성분은 catechin(1), epi-catechin(2), tyrosol(3) 및 tiliroside(4)이다. 분리 성분의 항산화활성은 DPPH 라디칼 및 superoxide 음이온 라디칼 소거활성을 이용하여 측정하였다. 화합물 1, 2, 3, 4는 $100{\mu}L$/mL 농도에서 각각 94.2 %, 93.4 %, 33.6 %, 11.2 %의 DPPH 라디칼 저해활성을 나타내었다. 또한, 화합물 1, 2, 3, 4는 $200{\mu}L$/mL 농도에서 각각 60.2 %, 35.1 %, 20.6%, 4.5 %의 superoxide 음이온 라디칼 저해활성을 나타내었다. 화합물 $1{\sim}4$는 개가시나무에서는 처음으로 분리된 물질이다.

• 대한한의학회지
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• 제22권3호
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• pp.31-41
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• 2001

Objectives : This study was performed to investigate the effects of Cordyceps Sinensis (CS) and Cordyceps Militaris (CM) on anti oxidation in the livers of ${CCl_4}-treated$ rats. Methods : Hepatotoxicity in rats was induced by carbon tetracWoride. $CCl_4-induced$ rats were administered with the extract of CS and CM. Results : In vitro, CS and CM didn't affect levels of lipid peroxide and the activities and type conversion ratio of xanthine oxidase. However, hydroxyl radicals and DPPHL radicals were decreased. In vivo, in the ${CCl_4}-treated$ rats, lipid peroxide, the activities and type conversion ratio of xanthine oxidase and superoxide radicals were increased but superoxide dismutase was decreased. After CS and CM were administered to ${CCl_4}-treated$ rats, levels of lipid peroxide, the activities and type conversion ratio of xanthine oxidase and superoxide radicals were decreased but superoxide dismutase was increased. Conclusions : These results suggest that CS and CM decrease the activities of free-radical-generating enzymes which form lipid peroxide and increase the activities of oxygen free radical scavenging enzymes.