• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.615-620
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• 2003

Gyrodinium impudicum strain KG03의 세포외 다당류인 p-KG03은 황함유 다당류로 항바이러스 검색 결과, 조사대상 바이러스 중 특히 뇌심근 경색바이러스 (encephalomyocarditis virus; EMCV)에 항바이러스 활성을 가지는 것으로 조사되었다.

• Applied Biological Chemistry
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• 제42권2호
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• pp.140-146
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• 1999

청각(1.2 kg)을 0.8% 염산 수용액(24 l)으로 추출한 추출물을 메탄올 환류, 에탄올 침전 및 투석하여 활성이 약 2배 증가된 조다당(CF-1)으로부터 2종의 항응고활성 다당류를 정제하였다. 정제는 CF-1의 DEAE-Toyopearl이온교환 크로마토그래피와 Sephadex G-75, Sephadex G-100, Sepharose CL-6B 겔여과 크로마토그래피, HPLC 등을 이용하였다. 최종 정제 다당류획분인 CF-1-VIa-1과 CF-1-VIIa-1는 분자량이 각각 80,000과 40,000 Da 이었으며, 주구성당으로서 arabinose와 galactose가 약 2:1의 몰비율로 풍부하게 함유되어 있었고, 구성당 잔기에 $12{\sim}13%$의 유황을 함유하는 함황성 다당류들이었다. CF-1-VIa-1와 CF-1-VIIa-1의 항응고활성을 $2.5\;{\mu}g/mL$의 농도에서 비교하였을 때 APTT활성은 대조군에 비하여 각각 262초, 250초이었고 TT활성은 각각 130초, 70초이었으며, 분자량이 큰 CF-1-VIa-1이 다소 높은 항응고활성을 보였다. CF-1-VIa-1와 CF-1-VIIa-1의 desulfation과 sulfation을 통한 항응고활성을 비교한 결과 desulfation시 항응고활성이 각각 약 80%와 50%로 감소하였으나 sulfation시에는 약 30%와 20%로 그 활성이 증가하였다. 두 다당류는 헤파린과 달리 농도 의존적으로 불용성 피브린의 형성을 억제하므로써 antithrombin III 비의존적 트롬빈 저해활성을 나타내었으며, 칼슘이온의 킬레이트에 의한 혈액응고 저해효과는 나타내지 않았다.

• Preventive Nutrition and Food Science
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• 제12권2호
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• pp.65-73
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• 2007

Sargassum fulvellum, a marine brown alga, is a popular low priced edible plant in Korean markets. The polysaccharide fraction of the alga was separated and investigated for its radical scavenging activities and the results compared with those of commercial fucoidans (Fucus vesiculosus and Undaria pinnatifida), BHA and ${\alpha}$-tocopherol. The polysaccharide fraction of S. fulvellum showed a promising DPPH radical scavenging activity than did other fucoidans. Moreover, the sample exhibited a dose-dependent activity on hydrogen peroxide scavenging activity in the V79-4 cell line. Interestingly, all the tested polysaccharide counterparts were more potent NO. scavengers than were the commercial antioxidants, BHA and ${\alpha}$-tocopherol. The sulfated polysaccharide of S. fulvellum had an approximate molecular weight of 529 kDa and mainly consisted of fucose and galactose, and minor amounts of mannose, rhamnose and xylose.

• ALGAE
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• 제31권4호
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• pp.403-414
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• 2016

The use of ionizing radiation and radioactive elements is becoming increasingly popular with the rapid developments in nuclear technology, radiotherapy, and radio diagnostic methods. However, ionizing radiation can directly or indirectly cause life-threatening complications such as cancer, radiation burns, and impaired immunity. Environmental contamination with radioactive elements and the depletion of ozone layer also contribute to the increased levels of radiation exposure. Radioprotective natural products have particularly received attention for their potential usefulness in counteracting radiation-induced damage because of their reduced toxicity compared with most drugs currently in use. Moreover, radioprotective substances are used as ingredients in cosmetic formulations in order to provide protection against ultraviolet radiation. Over the past few decades, the exploration of marine algae has revealed the presence of radioprotective phytochemicals, such as phlorotannins, polysaccharides, carotenoids and other compounds. With their promising radioprotective effects, marine algae could be a future source for discovering potential radioprotective substances for development as useful in therapeutics.

• 한국해양바이오학회지
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• 제7권2호
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• pp.71-78
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• 2015

Sulfated polysaccharides from plants or seaweed are well known for antioxidant, anticoagulant activity or other biological activities. The aim of the present study is to optimize the condition for the isolation of water soluble polysaccharides (WSP) and evaluate the biological activity. WSP was isolated from defatted microalgae B. braunii using chlorosulfonic acid or sulfuric acid. Among WSP isolated using sulfuric acid showed the lowest the content of total carbohydrates (g/L), whereas showed the highest antioxidant activity. Also the preliminary structural analysis of WSP was performed by FT-IR spectroscopy analysis.

• 대한화학회지
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• 제46권2호
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• pp.151-156
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• 2002

Fucoidan은 갈조류에 다량 함유되어있는 다당류로서 항암작용, 항혈응고작용, 항혈전작용, 항염증작용, 항virus작용과 같은 생리활성작용력을 가지고 있어 최근 많은 연구가 수행되어지고 있다. 본 논문은 갈조류에서 fucoidan을 추출 하여 그 항암활성을 SV40 DNA replication assay, RPA-ssDNA binding assay, 그리고 MCF7 cell growth inhibition assay를 이용하여 알아보았으며 이 항암활성은 fucoidan의 황산기가 중요한 역할을 하고 있음을 알아내었다. 화학적으로 황산기의 함유량을 증가시킨 fucoidan이 황산기의 함유량을 제거시킨 fucoidan보다 항암활성이 뛰어났고, 이는 RPA의 ssDNA 결함력을 떨어 EM리기 때문이라 예측되어 진다. 본 연구는 한국산 갈조류에서 추출한 황산기를 함유한 fucoidan의 항암활성능력을 보여주었다.

• Nutrition Research and Practice
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• 제11권1호
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• pp.3-10
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• 2017

BACKGROUND/OBJECTIVES: Sargassum horneri is an edible brown alga that grows in the subtidal zone as an annual species along the coasts of South Korea, China, and Japan. Recently, an extreme amount of S. horneri moved into the coasts of Jeju Island from the east coast of China, which made huge economic and environmental loss to the Jeju Island. Thus, utilization of this biomass becomes a big issue with the local authorities. Therefore, the present study was performed to evaluate the anti-inflammatory potential of crude polysaccharides (CPs) extracted from S. horneri China strain in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. MATERIALS/METHODS: CPs were precipitated from S. horneri digests prepared by enzyme assistant extraction using four food-grade enzymes (AMG, Celluclast, Viscozyme, and Alcalase). The production levels of nitric oxide (NO) and pro-inflammatory cytokines, including tumor necrosis factor (TNF)-${\alpha}$ and interleukin (IL)-$1{\beta}$ were measured by Griess assay and enzyme-linked immunosorbent assay, respectively. The levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), nuclear factor (NF)-${\kappa}B$, and mitogen-activated protein kinases (MAPKs) were measured by using western blot. The IR spectrums of the CPs were recorded using a fourier transform infrared spectroscopy (FT-IR) spectrometer. RESULTS: The polysaccharides from the Celluclast enzyme digest (CCP) showed the highest inhibition of NO production in LPS-stimulated RAW 264.7 cells ($IC_{50}$ value: $95.7{\mu}g/mL$). Also, CCP dose-dependently down-regulated the protein expression levels of iNOS and COX-2 as well as the production of inflammatory cytokines, including TNF-${\alpha}$ and IL-$1{\beta}$, compared to the only LPS-treated cells. In addition, CCP inhibited the activation of NF-${\kappa}B$ p50 and p65 and the phosphorylation of MAPKs, including p38 and extracellular signal-regulated kinase, in LPS-stimulated RAW 264.7 cells. Furthermore, FT-IR analysis showed that the FT-IR spectrum of CCP is similar to that of commercial fucoidan. CONCLUSIONS: Our results suggest that CCP has anti-inflammatory activities and is a potential candidate for the formulation of a functional food ingredient or/and drug to treat inflammatory diseases.

• Preventive Nutrition and Food Science
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• 제10권4호
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• pp.344-348
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• 2005

Fucoidans, high-molecular-weight sulfated polysaccharides extracted from brown seaweeds, have various biological activities. Here we examined whether fucoidan could ncrease the immunomodulation and proliferation capacity of cells in vitro. When peritoneal macrophages were treated with various concentrations of fucoidan $(1\~100{\mu}g/mL)$ for 24 hours, NO production was significantly increased. In addition, exposure of macrophages to $10{\mu}g/mL$ of fucoidan induced a phagocytic activity. Treatment of neuroblastoma cells (SK-N-SH) with fucoidan enhanced cell proliferation and NO production in a concentration-dependent manner. These results indicate that fucoidan has both immunomodulatory and cell proliferative properties, and may thus be a candidate for development as an immunomodulating agent.

• 한국수산과학회지
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• 제32권4호
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• pp.495-500
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• 1999

[ $\lambda$ ]-carrageenan 고유의 특성을 유지하면서 용해성을 높인 저분자 $\lambda$-carrageenan을 제조하여 보다 효율적으로 식품산업에 이용하기 위하여 초음파를 이용하여 $\lambda$-carrageenan을 저분자화하는 최적조건을 조사한 결과 $\lambda$-carrageenan의 초음파 처리시 각 처리조건들과 저분자화 효과와의 상관관계를 나타내는 반응표면식을 구한 결과, carrageenan 농도와 초음파 강도가 저분자화에 미치는 주 영향인자인 것으로 나타났다. 그리고 저분자화를 위한 초음파 처리의 최적 조건은 초음파 처리온도 $10^{\circ}C$, 초음파 강도 121.64 $W/cm^2$, 시료 carrageenan 농도 $2\%$, 초음파 처리시간 40분이었다. $\lambda$-carrageenan 및 저분자화된 $\lambda$-carrageenan의 분자량을 조사한 결과 대조구 $\lambda$-carrageenan의 분자량은 약 250,000이었으며 초음파처리에 따른 저분자화 된 $\lambda$-carrageenan의 분자량은 각각 p-1의 경우 약 200,000정도였으며, p-2의 경우 약 60,000정도이었다.

• Preventive Nutrition and Food Science
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• 제12권2호
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• pp.74-78
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• 2007

Fucoidan, that is high-molecular-weight sulfated polysaccharides extracted from brown seaweeds has been shown to elicit various biological activities. Here, we investigated the effects of fucoidan on cell proliferation and nitric oxide (NO) production in neuronal blastoma cell (SH-SY5Y). In the present study, we demonstrated that fucoidan treatment resulted in increase of cell proliferation and NO production. When cells were treated with amyloid-${\beta}$ (A${\beta}$) in the absence or presence of fucoidan, fucoidan recovered the cell viability decreased by A${\beta}$ peptides. To further determine whether nitric oxide synthase (NOS) is involved in proliferative effect of fucoidan, cells were treated with NOS inhibitors in the absence or presence of fucoidan. Selective constitutive nitric oxide synthase (cNOS) inhibitor, diphenylene iodonium chloride (DPI), caused a decrease of cell viability, whereas cell viability was increased by specific inducible nitric oxide synthase (iNOS) inhibitor, S-methylisothiourea (SMT), in the fucoidan-untreated cells. Treatment with fucoidan inhibited the cell viability decreased in DPI-exposed cells. In contrast, fucoidan had no effect on cell growth in SMT-treated cells, indicating that cNOS may not play a role in the proliferation of fucoidan-treated cells. The present data suggest that fucoidan has proliferative and neuroprotective effects and these effects may be associated with iNOS.