• Title/Summary/Keyword: substitution

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Mapping Grain Weight QTL using Near Isogenic Lines from an Interspecific Cross (벼 종간잡종 유래 근동질 유전자계통 이용 종자중 관여 유전자 분석)

  • Kang, Ju-Won;Yang, Paul;Yun, Yeo-Tae;Ahn, Sang-Nag
    • Korean Journal of Breeding Science
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    • v.43 no.4
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    • pp.304-310
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    • 2011
  • In previous studies, we reported QTLs for grain weight (GW), qGW3 and for spikelets per panicle (SPP), qSPP3 linked to RM60 on chromosome 3 using advanced backcross lines derived from a cross between Oryza sativa ssp. Indica cv. Milyang 23 and O. glaberrima. The O. glaberrima alleles at this locus increased GW and spikelets per panicle in the Milyang 23 background. To further confirm and narrow down the position of the QTLs on chromosome 3, substitution mapping was performed using five lines containing the target O. glaberrima segment on chromosome 3. The size and position of the O. glaberrima segment on chromosome 3 were different in each line. These lines possessed 3-10 non-target O. glaberrima introgressions in the Milyang 23 background. These five lines were evaluated for seven agronomic traits including 1,000 grain weight and spikelets per panicle and also genotyped with seven SSR markers. Four lines were informative in delimiting the position of QTLs, qGW3 and qSPP3. Two lines with the O. glaberrima segment flanked by SSR markers, RM60 and RM523 displayed significantly higher values than Milyang 23 in GW and SPP whereas two lines without that O. glaberrima segment displayed no difference in GW and SPP compared to Milyang 23. The result indicates that two QTL, qGW3 and qSPP3 are located in the interval between RM60 and RM523 which are 1.2-Mb apart. Introgression lines having QTLs, qGW3 and qSPP3 would be useful materials not only to indentify the relationship between these two yield QTLs, but also to develop high yielding variety via marker-aided selection technology.

Preparation of MA-PLA Using Radical Initiator and Miscibility Improvement of PLA/PA11 Blends (라디칼 개시제를 이용한 MA-PLA 제조 및 바이오플라스틱 PLA/PA11 블렌드의 상용성 개선)

  • Lee, Jong-Eun;Kim, Han-Eol;Nam, Byeong-Uk
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.4
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    • pp.76-85
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    • 2019
  • Recently, various investigation of vegetable oil which is extracted from natural resources is being progressed because of its low cost and environmental aspect. However, double bonds in vegetable oil should be substituted to other high reactive functional group due to its low reactivity for synthesizing bio-polymeric materials. ${\alpha}$-eleostearic acid, which is consist of conjugated triene, is the main component of tung oil, and the conjugated triene allows tung oil to have higher reactivity than other vegetable oil. In this study, tung oil is copolymerized with styrene and divinylbenzene to make thermoset resin without any substitution of functional group. Thermal and mechanical properties are measured to investigate the effects of the composition of each monomer on the synthesized thermoset resin. The result shows that the products have only one Tg, which means the synthesized thermoset resins are homogeneous in molecular level. Mechanical properties show that tung oil act as soft segment in the copolymer and make more elastic product. On the other hand, divinylbenzene acts as hard segment and makes more brittle product.

Synthesis and Characterization of Alkoxy and Alkylamino GAP Copolymer for Energetic Thermoplastic Elastomer (ETPE) (에너지화 열가소성 탄성체에 사용될 수 있는 알콕시 계열과 알킬 아민 계열 GAP Copolymer의 합성 및 분석)

  • Lim, Minkyung;Jang, Yoorim;Kim, Hancheul;Rhee, Hakjune;Noh, Sitae
    • Applied Chemistry for Engineering
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    • v.30 no.1
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    • pp.81-87
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    • 2019
  • In this study, synthetic methods and physical properties for a new class of glycidyl azide polymer (GAP) were investigated for energetic thermoplastic elastomers (ETPE). Four kinds of GAP copolymer polyols were synthesized by introducing nucleophiles such as azide, alkoxide and alkyl amine into poly(epichlorohydrin) (PECH). The GAP copolymer synthetic reaction can be evaluated as an environmental benign and efficient synthetic method due to the simultaneous one-step reaction using two kinds of nucleophiles and the complete consumption of sodium azide. The relative stoichiometric substitution ratio analysis and the progress of reaction were checked and monitored by inverse gated decoupled $^{13}C$ NMR and Fourier transform infrared (FT-IR) spectroscopy. The glass transition temperature and molecular weight were measured by differential scanning calorimetry (DSC) and gel permeation chromatography (GPC) analysis. The synthesized poly($GA_{0.8}-butoxide_{0.2}$), poly($GA_{0.7}-n-butylamine_{0.3}$), poly($GA_{0.7}-dipropylamine_{0.3}$) and poly($GA_{0.7}-morpholine_{0.3}$) had a glass transition temperature ranged from -39 to $-26^{\circ}C$.

Sequence variation of necdin gene in Bovidae

  • Peters, Sunday O.;Donato, Marcos De;Hussain, Tanveer;Rodulfo, Hectorina;Babar, Masroor E.;Imumorin, Ikhide G.
    • Journal of Animal Science and Technology
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    • v.60 no.12
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    • pp.32.1-32.10
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    • 2018
  • Background: Necdin (NDN), a member of the melanoma antigen family showing imprinted pattern of expression, has been implicated as causing Prader-Willi symptoms, and known to participate in cellular growth, cellular migration and differentiation. The region where NDN is located has been associated to QTLs affecting reproduction and early growth in cattle, but location and functional analysis of the molecular mechanisms have not been established. Methods: Here we report the sequence variation of the entire coding sequence from 72 samples of cattle, yak, buffalo, goat and sheep, and discuss its variation in Bovidae. Median-joining network analysis was used to analyze the variation found in the species. Synonymous and non-synonymous substitution rates were determined for the analysis of all the polymorphic sites. Phylogenetic analysis were carried out among the species of Bovidae to reconstruct their relationships. Results: From the phylogenetic analysis with the consensus sequences of the studied Bovidae species, we found that only 11 of the 26 nucleotide changes that differentiate them produced amino acid changes. All the SNPs found in the cattle breeds were novel and showed similar percentages of nucleotides with non-synonymous substitutions at the N-terminal, MHD and C-terminal (12.3, 12.8 and 12.5%, respectively), and were much higher than the percentage of synonymous substitutions (2.5, 2.6 and 4.9%, respectively). Three mutations in cattle and one in sheep, detected in heterozygous individuals were predicted to be deleterious. Additionally, the analysis of the biochemical characteristics in the most common form of the proteins in each species show very little difference in molecular weight, pI, net charge, instability index, aliphatic index and GRAVY (Table 4) in the Bovidae species, except for sheep, which had a higher molecular weight, instability index and GRAVY. Conclusions: There is sufficient variation in this gene within and among the studied species, and because NDN carry key functions in the organism, it can have effects in economically important traits in the production of these species. NDN sequence is phylogenetically informative in this group, thus we propose this gene as a phylogenetic marker to study the evolution and conservation in Bovidae.

The Effects of Renewable Energy in Agricultural Sector (농업분야 신재생에너지 보급현황 및 파급효과 분석)

  • Park, Jiyun;Kim, Yeonjoong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.1
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    • pp.224-235
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    • 2019
  • The increase in the amount of energy used in the agricultural sector due to the expansion of agricultural machinery and greenhouse horticulture has caused a range of problems, such as an increase in the cost of input, such as heating costs and greenhouse gas emission. To overcome these problems, this study examined the current status of energy use in greenhouse horticulture as well as the change patterns of non-taxable oil and agricultural electricity, and reviewed the current status of the supply of renewable energy and energy saving facilities for agriculture. This study investigated the area of advanced and renewable energy and energy saving facilities implemented, applied the energy saving ratio of advanced and renewable energy and energy saving facilities, and determined the effects of renewable energy in the agricultural sector, such as increase in production, decrease in heating cost, reduction in Government financial expenditure, reduction in greenhouse gas emission, and oil substitution effect.

Water Management Vulnerability Assessment Considering Climate Change in Korea (기후변화에 따른 우리나라 물 관리의 취약성 평가)

  • Kim, Yeon-Kyu;Yoo, Jeong-A;Chung, Eun-Sung
    • Journal of Climate Change Research
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    • v.3 no.1
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    • pp.1-12
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    • 2012
  • In order to use as basic data of adaptation, this study focused on a 'Water management vulnerability estimation' in Korea. Vulnerability is estimated dividing into flood mitigation and water resource management. Temporal resolution is 2000 year and the future 2020 year, 2050 year, 2100 year via A1B scenario. Time series data was normalized. Then weight that is gotten through delphi investigation was multiplied. Vulnerability is calculated through this process. In flood mitigation vulnerability, it was estimated to adaptation ability affect relatively biggest influence. In future, some area of Gangwon-do was analyzed that the flood mitigation vulnerability increases. In water resource management, it was estimated to climate exposure affect relatively biggest influence. At 2020 yr, there is a trend toward increased in the Chungcheongbuk-do and DaeJeon, Daegu, some area of Gyeongsangnamdo. Because this study evaluate relative vulnerability of whole country and analyzed spatial distribution, when local government establishes climate change adaptation details enforcement countermeasure, this study can give help to grasp whether should invest more in some field.

Evaluation of Replacement Ratio of Organic Fertilizers for Basal Application of Nitrogen Fertilizer in Pot Cultivation of Rice (벼 재배 시 유기질비료의 질소 밑거름 대체량 평가)

  • Kim, Myung-Sook;Kim, Seok-Cheol;Park, Seong-Jin;Lee, Chang-Hoon
    • Journal of the Korea Organic Resources Recycling Association
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    • v.27 no.1
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    • pp.5-14
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    • 2019
  • The objective of this study was investigated the effect of organic fertilizer(OF) application on the grain yield, protein content, and soil chemical properties in rice cultivation to evaluate the replacement ratio of OF based on basal fertilization of inorganic(nitrogen) fertilizer, In case of soil 1 with optimum range of organic matter for rice growth, the grain yield of rice in 70% and 100% treatments of OF were higher than the other treatments. In soil 2 with higher than the optimum range of soil organic matter, the grain yield of rice of NPK, 30%, 70%, 100%, and 300% treatments of OF showed no significant difference. When the protein criteria of rice quality was below 7%, the treatment of 30%, 70%, and 100% using OF in soil 1 were satisfied with protein criteria of rice. In case of soil 1, ammonium nitrogen content, electrical conductivity, and the organic matter in 30%, 70%, 100%, 300% treatments of OF showed similar tendency to NPK treatment. These results suggested that the substitution ratio of OF 70~100% could be appropriate to increase the yield and commercial quality for rice.

Synthesis of tung oil-based thermoset resin and its thermal·mechanical properties (Tung oil을 이용한 바이오 기반 열경화성 수지 합성 및 이의 열적·물리적 특성 연구)

  • Kim, Han-Eol;Lee, Jong-eun;Nam, Byeong-Uk
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.11
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    • pp.24-30
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    • 2018
  • Various investigations of vegetable oil extracted from natural resources are underway because of their low cost and environmental value. On the other hand, the double bonds in vegetable oil should be substituted to other high reactive functional groups due to their low reactivity for synthesizing bio-polymeric materials. ${\alpha}$-eleostearic acid, which consists of a conjugated triene, is the main component of tung oil, and the conjugated triene allows tung oil to have higher reactivity than other vegetable oils. In this study, tung oil was copolymerized with styrene and divinylbenzene to make a thermoset resin without any substitution of functional groups. The thermal and mechanical properties were measured to examine the effects of the composition of each monomer on the synthesized thermoset resin. The results showed that the products have only one Tg, which means the synthesized thermoset resins are homogeneous at the molecular level. The mechanical properties show that tung oil acts as a soft segment in the copolymer and makes a more elastic product. On the other hand, divinylbenzene acts as a hard segment and makes a more brittle product.

Evaluation of Apparent Chloride Diffusion Coefficient of Fly Ash Concrete by Marine Environment Exposure Tests (해양 환경 폭로 시험을 통한 FA 콘크리트의 겉보기 염화물 확산계수 평가)

  • Yoon, Yong-Sik;Lim, Hee-Seob;Kwon, Seung-Jun
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.23 no.3
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    • pp.119-126
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    • 2019
  • In case of RC(Reinforced Concrete) structures which are constructed in coastal areas, chloride ions in sea water corrode the steel rebar in concrete. Especially in coastal areas, RC structures are affected by not only immersion of sea water, but also tidal of sea water and airborne chloride ions. In this study, marine environment exposure tests are conducted, considering 3 types of exposure environments(immersion zone, tidal zone, splash zone) and the exposure periods of 180 days, 365 days, and 730 days. Also, the concrete mixtures for this study are established, considering 3 levels of W/B(Water to Binder) ratio(0.37, 0.42, 0.47) and 2 levels of substitution rate of Fly ash(0 %, 30 %). In all exposure environments, Fly ash concrete has lower apparent chloride diffusion coefficients than OPC concrete. It is thought that fly ash's pozzolan reaction improves chloride resistance of concrete. Fly ash concrete has up to 63.5 % of decreasing rate in 180 days of exposure and up to 55.8 % of decreasing rate in 730 days of exposure, based on diffusion coefficients of OPC concrete. As a result of evaluation about effects of exposure environments, apparent chloride diffusion coefficients of fly ash concrete are evaluated in order of tidal zone, immersion zone, and splash zone. In tidal zone, It is thought that repeated cycles of wetting and drying of sea water cause the diffusion of chloride ions rapidly.

A Preliminary Discussion on Policy Decision Making of AI in The Fourth Industrial Revolution (4차 산업혁명시대 인공지능 정책의사결정에 대한 탐색적 논의)

  • Seo, Hyung-Jun
    • Informatization Policy
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    • v.26 no.3
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    • pp.3-35
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    • 2019
  • In the fourth industrial revolution age, because of advance in the intelligence information technologies, the various roles of AI have attracted public attention. Starting with Google's Alphago, AI is now no longer a fantasized technology but a real one that can bring ripple effect in entire society. Already, AI has performed well in the medical service, legal service, and the private sector's business decision making. This study conducted an exploratory analysis on the possibilities and issues of AI-driven policy decision making in the public sector. The three research purposes are i) could AI make a policy decision in public sector?; ii) how different is AI-driven policy decision making compared to the existing methods of decision making?; and iii) what issues would be revealed by AI's policy decision making? AI-driven policy decision making is differentiated from the traditional ways of decision making in that the former is represented by rationality based on sufficient amount of information and alternatives, increased transparency and trust, more objective views for policy issues, and faster decision making process. However, there are several controversial issues regarding superiority of AI, ethics, accountability, changes in democracy, substitution of human labor in the public sector, and data usage problems for AI. Since the adoption of AI for policy decision making will be soon realized, it is necessary to take an integrative approach, considering both the positive and adverse effects, to minimize social impact.