• 한국식물병리학회지
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• 제14권6호
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• pp.583-588
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• 1998

This study was conducted to investigate the potential of nitrate-nonutilizing mutants (nit mutants) in ecological studies of Fusarium disease of strawberry. Nit mutants of Fusarium oxysporum from strawberry were easily formed on chlorate-containing media. Nit mutants were assigned to three phenotypic classes, nit1, nit3, and NitM, on the basis of their growth on media containing one of the following five different nitrogen sources ; nitrate, nitrite, hypoxanthine, ammonium and uric acid. Frequency of nit mutation and proportion of three phenotypes of nit mutants depended on the isolate. Mutation rate was 45.6% and ranged from 15.0% to 95.0%. The frequency of nit1 mutants was higher than that of nit3 or NitM. The complementary reaction between nit1 and NitM was higher than that of other combination. There has been no complementary response observed between nit3 and nit3. The nit mutants showed similar growth pattern as the that of wild type isolate on potato sucrose agar and potato sucrose liquid media. Most of the mutants retained pathogenicity, and maintained their phenotypes even after two year preservation through subculture on slanted PSA at room temperature. Nit mutants were selctively isolated from infested soil and infected plants on the selective medium (MMCPA) containing potassium chlorate with their original phenotypes, while naturally occurring isolates of Fusarium oxysporum were not grow on the medium. On the contrary, nit mutants showed very slight growth on the medium (MMPA) containing nitrate as a sole nitrogen source, and therefore could be distinguished from wild type isolate.

• 식물조직배양학회지
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• 제26권3호
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• pp.143-148
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• 1999

당근의 배양세포로부터 체세포배의 발생과정에 미치는 아스콜빈산 및 dehydroascorbic acid의 영향을 밝히기 위하여 본 실험을 시도하였다. 비배발생세포의 배양에 처리된 아스콜빈산은 세포증식을 촉진시켰을 뿐인데 dehydroascorbic acid는 세포증식을 억제시키면서 배발생세포로 전환시킨 효과가 있었다. 배발생세포의 배양에 처리된 아스콜빈산은 체세포배 발생을 억제시켰지만 dehydroascorbic acid는 체세포배발생을 촉진시켰다. 그러나 이와 같은 발생촉진은 구상배에서 중단되므로 성숙에는 오히려 저해적이었다. 이상의 결과로부터 당근의 캘러스배양에 dehydroascorbic acid를 처리하여 빠른 시일내에 배발생캘러스를 확보한 다음 dehydroascorbic acid 첨가 배발생 배지에서 초기배발생기간 배양 후 MS 기본배지로 옮겨 배양하면 고빈도의 체세포배생산 실험계가 확립될 것으로 판단된다.

• Journal of Plant Biotechnology
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• 제7권3호
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• pp.175-181
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• 2005

This work described some essential factors necessary for micro-propagation of banana for mass production of synthetic seeds for germ plasm conservation, and how peroxides activity of conserved tissue was influenced. Shoot tips of field grown plants were used to obtain shoot clusters on shoot proliferation medium (MS medium supplemented with 5 mg/l BAP). Using longitudinally-split shoot tip technique, 18720, 8640, 7488, 2016 plantlets were obtained from one shoot tip of Maghraby, Grand Naine, Balady, and Williams, respectively, in six subculture, one month each, on solid medium. Shoot tips excised from in vitro grown plantlets were encapsulated in calcium-alginate beads and stored at $4^{\circ}C$ for one month on half-strength MS basal medium without growth regulators or sugars. After one month all the viable-conserved synseeds formed shoots when they were transferred to MS basal medium, some of them showed synchronous formation of shoot and root systems in one week. Plants retrieved from encapsulated shoot tips were hardened off and transferred to soil.

• Plant Biotechnology Reports
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• 제3권4호
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• pp.341-345
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• 2009

This study describes culture conditions for a plant regeneration system via a combined pathway of somatic embryogenesis and organogenesis in root explant cultures of the commercial rose cultivar 'Charming'. Root explants formed white calluses at a frequency of 30% after 6 weeks of culture on Schenk and Hildebrandt (SH) medium supplemented with $11mg\;1^{-1}$ 2,4-dichlorophenoxyacetic acid. After 6 weeks of transfer to SH medium without growth regulators, initial white calluses gave rise to globular somatic embryos at a frequency of 2.8%, which were subsequently dedifferentiated to embryonic tissues. Somatic embryos or embryonic tissues initially derived from root explants did not undergo development beyond cotyledonary stage. To produce adventitious shoots, embryonic tissues were sliced and cultured on SH medium with $0.5mg\;1^{-1}$ 6-benzyladenine. After 4 weeks of culture, 28% of embryonic tissue explants formed adventitious shoots. Regenerated shoots were rooted on half strength SH medium with $0.1mg\;1^{-1}$ ${\alpha}-naphthalaneacetic$ acid and subsequently grown to maturity. Root-derived embryonic tissues were proliferated by subculture, while retaining the capacity for shoot production for a few years.

• 한국작물학회지
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• 제32권4호
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• pp.466-470
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• 1987

Hordeora spontaneum의 7계통의 trisomic 식물체에서 미숙배를 채취하여 2.4-D 1mg/$\ell$와 2mg/$\ell$가 첨가된 B5배지와 CI 배지에 치상하여 callus 형성을 유도했다. Callus 유기는 2.4-D 1mg/$\ell$ 첨가구에서는 B5배지보다 CI배지에서 양호하였으나, 2mg/$\ell$첨가구에서는 B5배지와 CI배지간에 차이가 없었다. 또 trisomic type에 따른 callus유기율에서도 차이는 인정되지 않았으며, 평균 Callus 유기율은 83 %였다. 식물체재생에서는 kinetin 1mg/$\ell$첨가구가 무첨가구보다 양호하고, 무첨가구에서는 B5배지보다 SI 배지가 좋은 경향을 나타냈으나 다같이 유의차는 없었다. 합계 46개체가 재생되었으며 그후 17개체가 엽녹소 결핍개체였고 재생율은 7.6%였다.

• 한국의류학회지
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• 제41권4호
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• pp.661-672
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• 2017

This research studies the aesthetic value of typography in fashion, within the framework of system for objective and multilateral research. For this purpose, a literature review and analysis of system and typography were performed. The scope of this research is from 2000 to 2015. The results of the research can be summarized as follows. First, by analyzing system and typography in modern fashion in order to set up a new theoretical standard, we found that the main values of typography in modern fashion concern functionality, hierarchy, stability, independency, and flexibility. Second, by analyzing of aesthetic value of typography in fashion within system, we found that main values the functionality of typography as a marketing tool, the hierarchy of typography as a slogan of subculture, the stability of typography for the increasing of collective belonging, the independency of typography as a linguistic playfulness, and the flexibility of typography as an image. Lastly, each aesthetic value of typography expressed in fashion has similarities and differences. It could be considered in the same vein as the system that always has a double-side character of dependency and independency, universality and individuality. This study has pursued to conduct more research on fashion within a framework of system.

• 한국환경농학회지
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• 제30권2호
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• pp.202-208
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• 2011

BACKGROUD: ${\alpha}$- and ${\beta}$- Endosulfan isomers of endosulfan, an endocrine disrupting chemical, are widely used cyclodiene organochlorine pesticide in worldwide, and it has widespread application in agriculture and can contaminate river-system as runoff from soil or aerial deposition METHOD AND RESULTS: In this study, an attempt was made to isolate an endosulfan degrading fungus from endosulfan-polluted agricultural soil. Through repetitive enrichment and successive subculture in media containing endosulfan and its metabolites as the sole carbon source, a fungus designated KEF-1 was isolated. Based on phylogenetic analysis, strain KEF-1 was assigned to the genus Eutypella. Also, the ITS (internal transcribed spacer) sequences of KEF-1 were submitted to GenBank under accession number EF581006. In potato dextrose broth containing 8 ${\mu}g$/mL endosulfan, strain KEF-1 completely degraded the endosulfanin 12 days. CONCLUSION: These results suggested that Eutypella sp. KEF-1 has potential as a biocatalyst for endosulfan bioremediation

• 자연과학논문집
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• 제8권2호
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• pp.147-152
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• 1996

딸기의 3품종을 엽과 엽병으로 배양한 결과 2,4-D + BA 처리에서는 치밀한 캘러스가 유기되었고 이 캘러스로부터 다수의 신초가 분화되었으며 NAA + BA 처리에서는 부숴지기 쉬운 캘러스가 유기되었다. '수홍' 엽조직을 NAA나 2,4-D가 첨가된 배지에서 8개월간 배양하면 212번 primer에서 생산된 RAPD band가 모본 band와는 상이하게 나타났다. '보교'와 '여봉'의 엽에서 유기된 캘러스를 2,4-D가 첨가된 액체배지에 계대배양회수를 달리하여 배양하였을 때 12회 계대배양에서 모본과 상이한 RAPD band가 출현되었다.

• Archives of Pharmacal Research
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• 제19권5호
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• pp.400-405
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• 1996

In vitro studies were conducted to dertermine the frequency rate of spontaneous resistance to LB20304 and to dertermine whether cross-resistance to other antimicrobial agents develops. In eight strains of bacteria, the frequency of mutation to LB20304 at the concentrations of four and eight times the minimal inhibitory concentration(MIC) ranaged from less than 4.0 ${\times}$ $10^{-10}$ to 2.2 $\{times}$ $10^{-10}$ . These results were similar to those founf for other new fluoroquinolones. THe development of stepwise resistance was determined by repeated subculture in broth in the presence of increasing concentration of the compounds. Exposure of bacteria to increasing concentrations of LB20304 resulted in the selection of organisms with higher MICs. There were 4- to 128-fold increases in the MIC of LB20304 for bacterial strains of Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli and Pseudomonas aeruginosa. However, those strains selected after repeated exposure were well within the susceptibility range for LB20304 except for Pseudomonas aeruginosa. The resistant isolates selected with LB20304 showed cross-resistance when tested against ciprofloxacin and vice versa.

• 복식문화연구
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• 제14권1호
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• pp.153-167
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• 2006

The hip-hop, which began with subculture of black people in Harlem, have changed variously in musical history after the times, which has become the culture phenomenon that had controlled dancing, fashion, and further consciousness of the new generation around the world in 1990s. Since Jean Patou first used Graffiti on clothing in 1920s, graffiti art has been expressed through various methods in many designers' works. The purpose of this study is to develop 'The Hip-Hop' clothing design which express major images in works of Jean-Michel Basquiat, a representative painter of Graffiti art in modern fashion. The concept is Jean Casual Look representing Young Street Culture. Breaking from existing strong legitimate hip-hop styles, clothing design expresses funny, healthy, comfortable, and active feelings. Adobe Illustrator and Adobe Photoshop program were used to design Hip-Hop clothing. With the result that the images on Basquiat's works applied to the hip-hop clothing design, it is suggested that the works of artists are worthy of practical use as factors of a high value-added design, which corresponds with collaboration appearing in modern design variously.