[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5338/KJEA.2011.30.2.202

Biodegradation of Organochlorine Insecticide Endosulfan by the Fungus Eutypella sp. KEF-1

Lee, Jung-Bok (Department of Optometry, Kundong University)
Park, Sang-Yeul (Department of Optometry, Kundong University)
Shin, Kee-Sun (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Jeon, Chun-Pyo (Dept. of Medicine Quality analysis, Andong Science College)
Kim, Jang-Eok (School of Applied Bioscience, Kyungpook National University)
Kwon, Gi-Seok (School of Bioresource Sciences, Andong National University)

Publication Information

Korean Journal of Environmental Agriculture / v.30, no.2, 2011 , pp. 202-208 More about this Journal

Abstract

BACKGROUD: ${\alpha}$ - and ${\beta}$ - Endosulfan isomers of endosulfan, an endocrine disrupting chemical, are widely used cyclodiene organochlorine pesticide in worldwide, and it has widespread application in agriculture and can contaminate river-system as runoff from soil or aerial deposition METHOD AND RESULTS: In this study, an attempt was made to isolate an endosulfan degrading fungus from endosulfan-polluted agricultural soil. Through repetitive enrichment and successive subculture in media containing endosulfan and its metabolites as the sole carbon source, a fungus designated KEF-1 was isolated. Based on phylogenetic analysis, strain KEF-1 was assigned to the genus Eutypella. Also, the ITS (internal transcribed spacer) sequences of KEF-1 were submitted to GenBank under accession number EF581006. In potato dextrose broth containing 8 ${\mu}g$ /mL endosulfan, strain KEF-1 completely degraded the endosulfanin 12 days. CONCLUSION: These results suggested that Eutypella sp. KEF-1 has potential as a biocatalyst for endosulfan bioremediation

Keywords

Biodegradation; Bioremediation; Detoxification; Endosulfan; Endosulfan sulfate; Eutypella sp.;

Citations & Related Records

Reference

1	White, T.F., Bruns, T., Lee, S. and Taylor, J., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocol: A Guide to Methods and Applications, Innis MA, Gelfand DH, Sninsky FS, and White TTed, pp. 315-322. Academic Press, San Diego, CA, USA.
2	Mukherjee, I. and Mittal, A., 2005. Bioremediation of endosulfan using Aspergillus terreus and Cladosporium oxysporum, Bull. Environ. Contamin. Toxicol. 1034-1040.
3	Paul, V. and Balasubramaniam, E., 1997. Effect of single and repeated administration of endosulfan on behavior and its interaction with centrally acting drugs in experimental animals: a mini review, Environ. Toxicol. Pharmacol. 3, 151-157. DOI ScienceOn
4	Palma, P., Pal, V.L., Matos, C., Fernandes, R.M. Bohn, A., Soares, A.M. and Barbosa, I.R., 2009. Effects of atrazine and endosulfansulphate on the ecdysteroid system of Daphnia magna, Chemosphere. 74, 576-581.
5	Ramamoorthy, K., Wang, F., Chen, I.C., Norris, J.D., McDonnell, D.P., Leonard, L.S., Gaido, K.W., Bocchinfuso, W.P., Korach, K.S. and Safe, S., 1997. Estrogenic activity of a dieldrin/toxaphene mixture in the mouse uterus, MCF-7 human breast cancer cells, and yeast-based estrogen receptor assays: no apparent synergism, Endocrinology. 138, 1520-1527. DOI ScienceOn
6	Sethunathan, N., Megharaj, M., Chen, Z., Singh, N., Kookana, R.S. and Naidu, R., 2002. Persistence of endosulfan and endosulfa sulfate in soil as affected by moisture regime and organic matter addition, Bill. Environ. Contam. Toxicol. 68, 725-731. DOI
7	Sethunathan, N., Megharaj, M., Chen, Z.L., Williams, B.D., Lewis, G. and Naidu, R., 2004. Algal degradation of a known endocrine disrupting insecticide, $\alpha$ -endosulfan, and its metabolite, endosulfan sulfate of liquid medium and soil, J. Agric. Food Chem. 52, 3030-3035. DOI ScienceOn
8	Shetty, P.K., Mitra, J., Murthy, N.B.K., Namitha, K.K., Savitha, K.N. and Raghu, K., 2000. Biodegradation of cyclodiene insecticide endosulfan by Mucor thermohyalospora MTCC 1384, Curr. Sci. 1381-1383.
9	Siddique, T., Benedict, C.O., Muhammad, A. and Frankenberger, Jr. W.T., 2003a. Biodegradation kinetics of endosulfan Fusarium ventricosum and a Pandoraea Species, J. Agric. Food Chem. 51, 8015- 8019. DOI
10	Kullman, S.W. and Matsumura, F., 1996. Metabolic pathways utilized by Phanerochaete chrysoporium for degradation of the cyclodiene pesticide endosulfan, Appl. Environ. Microbiol. 62, 593-600
11	Kumar, K., Devi, S.S., Krishnamurthi, K., Kanade, G.S. and Chakrabarti, T., 2007. Enrichment and isolation of endosulfan degrading and detoxifying bacteria, Chemosphere. 68, 317-322. DOI ScienceOn
12	Kwon, G.S., Kim, J.E., Kim, T.K., Sohn, H.Y., Koh, S.C., Shin, K.S. and Kim, D.G., 2002. Klebsiella pneumoniae KE-1 degrades endosulfan without formation of the toxic metabolite, endosulfan sulfate, FEMS Microbiol. Lett. 215, 255-259. DOI
13	Kwon, G.S., Sohn, H.Y., Shin, K.S., Kim, E. and Seo, B.I., 2005. Biodegradation of the organochlorine insecticide, endosulfan, and the toxic metabolite, endosulfan sulfate, by Klebsiella oxytoca KE-8, Appl. Microbiol. Biotechnol. 67, 845-850. DOI
14	Lee, S.E., Kim, J.S., Kennedy, I.R., Park, J.W., Kwon, G.S., Koh, S.C. and Kim, J.E., 2003. Biotransformation of an organochlorine insecticide, endosulfan, by Anabaena species, J. Agric. Food Chem. 51, 1336-1340. DOI ScienceOn
15	Shivaramaiah, H.M., Sanchez-Bayo, F., Al-Rifai, J., and Kennedy, I.R., 2005. The fate of endosulfan in water, J. Environ. Sci. Health Part B. 40, 711-720. DOI ScienceOn
16	Siddique, T., Zahir, A.Z. and William, T.F., 2003b. Revered-phase liquid chromatographic method for analysis of endosulfan and its major metabolites, J. Liqu. Chrom. Rel. Technol. 26, 1069-1082. DOI ScienceOn
17	Sohn, H.Y., Kwon, C.S., Kwon, G.S., Lee, J.B. and Kim, E. 2004. Induction of oxidative stress by endosulfan and protective effect of lipid-soluble antioxidants against endosulfan-induced oxidative damage, Toxicol. Lett. 151, 357-365. DOI ScienceOn
18	Sutherland, T.D., Weir, K.M., Lacey, M.J., Horne, I., Russell, R.J. and Oakeshott, J.G., 2002. Enrichment of microbial culture capable of degrading endosulphate, the toxic metabolite of endosulfan, J. Agric. Food Chem. 92, 541-548.
19	Thompson, J.D., Higgins, D.G. and Gibson, T.J., 1994. CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice, Nucleic Acid Res. 22, 4673- 4680. DOI
20	Turner, K.O., Syvanen, M. and Meizrl, S., 1997. The human acrosome reaction in highly sensitive to inhibition by cyclodiene insecticides, J. Androl. 11, 1469-1476.
21	Mukherjee, I. and Gopal, M., 1994. Degradation of b-endosulfan by Aspergillus niger, Toxicol. Environ. Chem. 46, 217-221. DOI ScienceOn
22	Lee, J.B., Shon, H.Y., Shin, K.S., Jo, M.S., Kim, J.E., Lee, S.W., Shin, J.W., Kum, E.J. and Kwon, G.S., 2006. Isolation of a Soil Bacterium Capable of Biodegradation and Detoxification of Endosulfan and Endosulfan Sulfate, J. Agric. Food Chem. 54, 8824-8828. DOI ScienceOn
23	Martens, R., 1976. Degradation of [8, 9- $^{14}C$ ] endosulfan by soil microorganisms, Appl. Environ. Microbiol. 6, 853-858.
24	Maier-Bode, H., 1968. Properties effect, residues, and analytics of the insecticide endosulfan, Residue. Rev. 22, 1-44.
25	Beck, E.W., Johnson, J.C., Soodham, D.W., Leuck, D.B., Dsway, L.H., Robbins, J.E. and Bowman, M.C., 1996. Residues of endosulfan in meat and milk of cattle fed treated forages, J. Econ. Entomol. 17, 216-220.
26	Bhalerao, T.S. and Puranik, P.R., 2007. Biodegradation of organochlorine pesticide, endosulfan, by a fungal soil isolate, Aspergillus niger, Int. Biodeter. Biodegr. 59, 315-321. DOI ScienceOn
27	Felsenstein, J., 1985. Confidence intervals on phylogenies: an approach using the bootstrap, Evolution. 39, 783-791. DOI ScienceOn
28	Lu, Y., Morimoto, K., Takechita, T., Takeuchi, T. and Saito, T., 2000. Genotoxic effects of $\alpha$ -endosulfan and $\beta$ - endosulfan on human HepG2 cells, Environ. Health Persp. 108, 559-561.
29	Karatas, A.D., Aygun, D.and Baydin, A., 2006. Characteristics of endosulfan poisoning: a study of 23 cases, Singapore Med. J. 47, 1030-1032.
30	Jin, L., Tran, D.Q., Ide, C.F., McLachlan, J.A. and Arnold, S.F., 1997. Several synthetic chemicals inhibit progesterone receptor-mediated transactivation in yeast, Biochem. Biophy. Res. Comm. 233, 139-146. DOI ScienceOn
31	Kimber, S.W.L., Southan, S.K., Ahmad, N. and Kennady, I.R., 1994. The fate of endosulfan sprayed on cotton. In Proceedings of the World Cotton Research Conference-Ion Challenging the Future, Constable GA and Forrester NW ed., pp. 589-594. CSIRO, Melboune, Australia
32	Kovacs, M.F., 1963. Thin layer chromatography for chlorinated pesticide residue analysis, J. Assoc. official. Agr. Chemists. 48, 1018-1022.