Abstract
Immature embryos of Hordeum spontaneum were cultured on B5 and CI medium (Cheng's modified MS) to induce callus formation. CI media containing lmg/$\ell$ and 2mg/$\ell$ 2,4-D were more effective than B5 medium with lmg/$\ell$ 2,4-D for the initiation of callus. Total 883 calli were induced from 1,060 immature embryos plated. Callus induction frequency was 83%. Calli were transferred to differentiation media after one subculture to regenerate plants. Forty six plants were regenerated from 608 calli. Seventeen plants were chlorophyll deficient. There was no significant difference for plant regeneration among genotypes and media effects in calli which had been induced from immature embryos of seven types of trisomies. The overall regeneration frequency was 7.6%.
Hordeora spontaneum의 7계통의 trisomic 식물체에서 미숙배를 채취하여 2.4-D 1mg/$\ell$와 2mg/$\ell$가 첨가된 B5배지와 CI 배지에 치상하여 callus 형성을 유도했다. Callus 유기는 2.4-D 1mg/$\ell$ 첨가구에서는 B5배지보다 CI배지에서 양호하였으나, 2mg/$\ell$첨가구에서는 B5배지와 CI배지간에 차이가 없었다. 또 trisomic type에 따른 callus유기율에서도 차이는 인정되지 않았으며, 평균 Callus 유기율은 83 %였다. 식물체재생에서는 kinetin 1mg/$\ell$첨가구가 무첨가구보다 양호하고, 무첨가구에서는 B5배지보다 SI 배지가 좋은 경향을 나타냈으나 다같이 유의차는 없었다. 합계 46개체가 재생되었으며 그후 17개체가 엽녹소 결핍개체였고 재생율은 7.6%였다.