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Antioxidant activities of germinated Ginseng(Panax ginseng C.A. Meyer) Seed

  • Mi-Ok Chae;So-Hyun Kim;Yong-Sung Park;Il-Doo Kim;Dong-Hyun Shin
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.332-332
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    • 2022
  • Ginseng has been traditionally used in Asia including Korea, for health care and to treat verities of different diseases such as immune disease, liver disease, and cancer. The current study was aim to unveal the most efficient method such heating, prethanol-A and ultrasound, for cured extraction of ginseng with higher antioxidant activity. The current results shows a significant improvement in the inhibition of H2O2 by the ultrasound method than the HT and Pre-A method. Thus this inhibition in free redical is possible through the increase in the antioxidant activity. Therefore in this study the CAT, APX and phenolic and flavonoid content was increased in ginseng seed and germinated ginseng sprouts by the US method, while the POD, SOD and GSH activity was increased in HT method. This suggest that the different extraction method in different stage of ginseng growth show a different biochemical and metabolites activation. Thereby the Ultrasound and Heat extraction was a feasible alternative method for extracting interested ingredients from biological materials.

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Restoration Report of Bomboo-Plated Inkstone Case (조선시대(朝鮮時代) 주칠죽장연함(朱漆竹裝硯函)의 수리(修理))

  • Yi, Yong-hee;Yu, Hei-sun;Kim, Kyoung-su
    • Conservation Science in Museum
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    • v.2
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    • pp.35-40
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    • 2000
  • Conservation Science Lab. of the National Museum of Korea recovered Bamboo-plated inkstone case which is used for keeping inkstick, inkstone and inkstone water container by using traditional lacquering and wooden art technology. Texture and construction method of wood, ingredients for lacquering and the method, and the design were analyzed in order to restore the original manufacturing method. Especially, perfect restoration of partially damaged lacquer surface is the most important aspect in restoring Bamboo plated inkstone case, therefore focus was put on investigation of surface lacquering. First, lacquering surface sample was taken off from the object and processed into flake and observed with transmission microscope in order to investigate lacquering method and the pigment in the lacquering surface was analyzed with XRD and SEM-EDS. The result showed that the object was decorated with thin-layered bamboo in the surface which is made of Royal paulownio. and lacquered several times with the mixture of Hematite(Fe2O3) and Cinnabar(HgS) in the remaining part.

Analysis of Selected Water-Soluble Vitamin B1, B2, B3, and B12 Contents in Namul (Wild Greens) Consumed in Korea (국내에서 섭취되는 나물류에 함유된 일부 수용성 비타민의 함량 분석)

  • Yoon, Jimin;Chung, Haejung;Kim, Younghwa
    • The Korean Journal of Food And Nutrition
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    • v.32 no.1
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    • pp.61-68
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    • 2019
  • In this study, the contents of water-soluble vitamins $B_1$ (thiamin), $B_2$ (riboflavin), $B_3$ (niacin), and $B_{12}$ (cyanocobalamin) in namuls (wild greens), such as salads and side dishes, consumed in Korea were quantified by high-performance liquid chromatography (HPLC) with UV and fluorescence detection. All samples were prepared with recipes used in Korea. All analyses were performed under the quality control of vitamin $B_1$, $B_2$, $B_3$, and $B_{12}$. The vitamin $B_1$, $B_2$, and $B_3$ contents in namuls consumed in Korea were analyzed. The highest content of vitamin $B_1$ was $3.018{\pm}0.016mg/100g$ in Putgochudoenjang-muchim. The highest contents of vitamin $B_2$, $B_3$, and $B_{12}$ were $0.279{\pm}0.003mg/100g$ in Gul-muchim, $12.241{\pm}0.040mg/100g$ in Chamchi-salad, and $8.133{\pm}0.371{\mu}g/100g$ in Pijogae-muchim, respectively. These results showed that animal-based ingredients in salads provided for good intake of vitamin $B_{12}$. These results can be used as basic data for food composition tables and improvement of the national health of Koreans.

In vitro gas and methane production of some common feedstuffs used for dairy rations in Vietnam and Thailand

  • N. T. D., Huyen;J. Th. Schonewille;W. F. Pellikaan;N. X. Trach;W. H. Hendriks
    • Animal Bioscience
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    • v.37 no.3
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    • pp.481-491
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    • 2024
  • Objective: This study determined fermentation characteristics of commonly used feedstuffs, especially tropical roughages, for dairy cattle in Southeast Asia. This information is considered relevant in the context of the observed low milk fat content and milk production in Southeast Asia countries. Methods: A total of 29 feedstuffs commonly used for dairy cattle in Vietnam and Thailand were chemically analysed and subjected to an in vitro gas production (GP) test. For 72 h, GP was continuously recorded with fully automated equipment and methane (CH4) was measured at 0, 3, 6, 9, 12, 24, 30, 36, 48, 60, and 72 h of incubation. A triphasic, nonlinear, regression procedure was applied to analyse GP profiles while a monophasic model was used to obtain kinetics related to CH4 production. Results: King grass and VA06 showed a high asymptotic GP related to the soluble- and non-soluble fractions (i.e. A1 and A2, respectively) and had the highest acetate to propionate ratio in the incubation fluid. The proportion of CH4 produced (% of GP at 72 h) was found to be not different (p>0.05) between the various grasses. Among the selected preserved roughages (n = 6) and whole crops (n = 4), sorghum was found to produce the greatest amount of gas in combination with a relatively low CH4 production. Conclusion: Grasses belonging to the genus Pennisetum, and whole crop sorghum can be considered as suitable ingredients to formulate dairy rations to enhance milk fat content in Vietnam/Thailand.

CO2 Adsorption of Amine Functionalized Activated Carbons

  • Meng, Longyue;Cho, Ki-Sook;Park, Soo-Jin
    • Carbon letters
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    • v.10 no.3
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    • pp.221-224
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    • 2009
  • In this work, the $CO_2$ adsorption behaviors of amine functionalized activated carbons (ACs) were investigated. The surface of ACs was modified with urea, melamine, diethylenetriamine (DETA), pentaethylenehexamine (PEHA), polyethylenimine (PEI), and 3-aminopropyl-triethoxysilane (ATPS). The various surface properties of amine functionalized ACs were characterized by Boehm's method, nitrogen full isotherms, XPS, and TGA analyses. The active ingredients impregnated on the ACs show significant influence on the adsorption for $CO_2$ and its volumes adsorbed on amine functionalized ACs are larger than that on the pristine ACs, which is due to the grafted amine groups of the AC surfaces.

ON A T-FUNCTION f(x)=x+h(x) WITH A SINGLE CYCLE ON ℤ2n

  • Rhee, Min Surp
    • Journal of the Chungcheong Mathematical Society
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    • v.24 no.4
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    • pp.927-934
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    • 2011
  • Invertible transformations over n-bit words are essential ingredients in many cryptographic constructions. When n is large (e.g., n = 64) such invertible transformations are usually represented as a composition of simpler operations such as linear functions, S-P networks, Feistel structures and T-functions. Among them we study T-functions which are probably invertible and are very useful in stream ciphers. In this paper we study some conditions on a T-function h(x) such that f(x) = x + h(x) has a single cycle on ${\mathbb{Z}}_{2^n}$.

Anti-oxidant and Anti-pollution Composition Containing the Extract of Nypa fruticans Wurmb, Saussurea neoserrata, Codium fragile and Enteromorpha compressa

  • Choi, Go-Eun;Lee, Gil-Hyun;Hyun, Kyung-Yae
    • Biomedical Science Letters
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    • v.26 no.3
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    • pp.157-163
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    • 2020
  • Long-term exposure to environmental pollutants can impair the human skin's barrier function and promote skin aging mechanisms. The visible consequences of these effects are dryness, wrinkles, black spots and worsening skin sensitivity. As awareness of the effects of environmental stressors on the skin has recently increased, consumers' demand for cosmetics that can provide anti-pollution effects is increasing. In this study, the possibility of anti-pollution cosmetic material was investigated by measuring the inhibitory effect of free radicals using a mixture of Nypa fruticans Wurmb (NF), Saussurea neoserrata (SN), Codium fragile (CF) and Enteromorpha compressa (EC), which has anti-inflammatory and antioxidant effects against irritation caused by dust. Combining the experimental results of a mixture of NF, SN, CF and EC by induction of fine dust stimulation, inhibition of inflammatory factors (PGE2) and intracellular free radicals (ROS) by inhibiting effects were significant, indicating the possibility of use as anti-pollution cosmetics. The most plants used as sources of anti-pollution cosmetic ingredients contain antioxidants as active substances. In our highly industrialized and chemically polluted world, it is not surprising that most plants used as sources of anti-pollution cosmetic ingredients contain antioxidants as active substances. Considering our results, it can be suggested that this anti-pollutant consisting of NF, SN, CF and EC may be a good ingredient for skincare products for the cosmetic industry due to their antioxidant properties, which may especially alter skin aging.

Study on the Change of Antioxidant Activity by Enzymatic Hydrolysis in Sophora japonica Linne, Houttuynia cordata Thunberg, Leonurus japonicus Houttuyn (괴화, 어성초, 익모초에서 효소 분해에 의한 항산화 활성 변화 연구)

  • Cha, Bae Cheon
    • Journal of Korean Medicine for Obesity Research
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    • v.21 no.1
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    • pp.1-9
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    • 2021
  • Objectives: The enzymatic hydrolysis is one of the processing methods that improve its effectiveness on medicinal herbs. In this research, changes in ingredients and activity by enzymatic hydrolysis were studied. Methods: For this study, a carbohydrate hydrolase such as viscozyme, which converts glycosides to aglycone, was applied to induce constituent changes in Sophora japonica Linne, Houttuynia cordata Thunberg and Leonurus japonicus Houttuyn. Changes in antioxidant activity were measured using the 1,1-diphenyl-2-picrylhydrazl (DPPH) method, and changes in ingredients were analyzed by high performance liquid chromatography. Results: As a result of enzymatic hydrolysis, the content of quercetin was increased from 1.26 mg/g to 29.66 mg/g in Sophora japonica Linne, from 0 mg/g to 0.66 mg/g in Houttuynia cordata Thunberg and from 0.43 mg/g to 0.71 mg/g in Leonurus japonicus Houttuyn. As a result of the antioxidant experimentation, the IC50 of Sophora japonica Linne decreased from 5 ug/ml (MeOH extract) and 9.1 ug/ml (EtOAc fraction) to 3.0 ug/ml, Houttuynia cordata Thunberg decreased from 15.6 ug/ml (MeOH extract) and 13.6 ug/ml (EtOAc fraction) to 11.2 ug/ml, and Leonurus japonicus Houttuyn decreased from 14.4 ug/ml (MeOH extract) and 12.6 ug/ml (EtOAc fraction) to 10.2 ug/ml. Conclusion: In conclusion, it was confirmed that glycoside rutin contained in the three medicinal herbs was changed to quercetin which is the aglycone, by the enzymatic hydrolysis using viscozyme. In terms of antioxidant activity, Sophora japonica Linne showed a significant antioxidant activity value that closes to the control group butylated hydroxyanisole. Houttuynia cordata Thunberg and Leonurus japonicus Houttuyn showed a minor increase in antioxidant activity.

Integration of virtual screening and proteomics reveals potential targets and pathways for ginsenoside Rg1 against myocardial ischemia

  • Rongfang Xie;Chenlu Li;Chenhui Zhong;Zuan Lin;Shaoguang Li;Bing Chen;Youjia Wu;Fen Hu;Peiying Shi;Hong Yao
    • Journal of Ginseng Research
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    • v.48 no.4
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    • pp.395-404
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    • 2024
  • Background: Ginsenoside Rg1 (Rg1) is one of the main active components in Chinese medicines, Panax ginseng and Panax notoginseng. Research has shown that Rg1 has a protective effect on the cardiovascular system, including anti-myocardial ischemia-reperfusion injury, anti-apoptosis, and promotion of myocardial angiogenesis, suggesting it a potential cardiovascular agent. However, the protective mechanism involved is still not fully understood. Methods: Based on network pharmacology, ligand-based protein docking, proteomics, Western blot, protein recombination and spectroscopic analysis (UV-Vis and fluorescence spectra) techniques, potential targets and pathways for Rg1 against myocardial ischemia (MI) were screened and explored. Results: An important target set containing 19 proteins was constructed. Two target proteins with more favorable binding activity for Rg1 against MI were further identified by molecular docking, including mitogen-activated protein kinase 1 (MAPK1) and adenosine kinase (ADK). Meanwhile, Rg1 intervention on H9c2 cells injured by H2O2 showed an inhibitory oxidative phosphorylation (OXPHOS) pathway. The inhibition of Rg1 on MAPK1 and OXPHOS pathway was confirmed by Western blot assay. By protein recombination and spectroscopic analysis, the binding reaction between ADK and Rg1 was also evaluated. Conclusion: Rg1 can effectively alleviate cardiomyocytes oxidative stress injury via targeting MAPK1 and ADK, and inhibiting oxidative phosphorylation (OXPHOS) pathway. The present study provides scientific basis for the clinical application of the natural active ingredient, Rg1, and also gives rise to a methodological reference to the searching of action targets and pathways of other natural active ingredients.

Investigation of Functional Ingredients from Onion According to the Extraction Methods, Heat Treatment, and Storage Period (양파의 추출방법과 추출액의 살균·저장에 따른 기능성성분 분석)

  • Park, Hoon;Oyunzul, Ganbud;Suh, Sung-Who;Park, Young-Seo;Jang, Jae-Kweon;Chung, Myong-Soo;Choi, Young Jin;Shim, Kun-Sub
    • Food Engineering Progress
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    • v.13 no.2
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    • pp.92-98
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    • 2009
  • An investigation was carried out to study the effects of extraction, heat treatment, and storage temperature on functional ingredients of onion. Extracts of onion paste and freeze dried onion were prepared by treatments with several solvents including hot water, methanol, and ethanol, and then contents of functional ingredients such as total phenol, flavonoid, quercetin, and thiosulfinate were determined. The highest levels of functional ingredients were observed in samples extracted using water at 100$^{\circ}C$ or ethanol. The skin extract contained 60-fold more quercetin than onion paste extract, whereas no thiosulfinate was detected in onion skin extract. Heat treatment of onion extracts at 60, 80, 105, and 121$^{\circ}C$ did not effect on the levels of all functional ingredients tested. During four-week storage, the levels of total phenol, flavonoid, and quercetin were not significantly changed regardless of storage temperature. However, the total thiosulfinate was rapidly reduced as the storage temperature increased.