• Food Engineering Progress
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• v.21 no.3
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• pp.233-241
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• 2017

Scaffolds of cell substrates are biophysical platforms for cell attachment, proliferation, and differentiation. They ultimately play a leading-edge role in the regeneration of tissues. Recent studies have shown the potential of bioactive scaffolds (i.e., osteo-inductive) through 3D printing. In this study, rice bran-derived biocomposite was fabricated for fused deposition modeling (FDM)-based 3D printing as a potential bone-graft analogue. Rice bran by-product was blended with poly caprolactone (PCL), a synthetic commercial biodegradable polymer. An extruder with extrusion process molding was adopted to manufacture the newly blended "green material." Processing conditions affected the performance of these blends. Bio-filament composite was characterized using field emission scanning electron microscopy (FE-SEM) and energy dispersive X-ray spectroscopy (EDX). Mechanical characterization of bio-filament composite was carried out to determine stress-strain and compressive strength. Biological behaviors of bio-filament composites were also investigated by assessing cell cytotoxicity and water contact angle. EDX results of bio-filament composites indicated the presence of organic compounds. These bio-filament composites were found to have higher tensile strength than conventional PCL filament. They exhibited positive response in cytotoxicity. Biological analysis revealed better compatibility of r-PCL with rice bran. Such rice bran blended bio-filament composite was found to have higher elongation and strength compared to control PCL.

• The Journal of the Convergence on Culture Technology
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• v.8 no.3
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• pp.265-270
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• 2022

The purpose of this study is to derive the damage weak part of the elastic rail fastening system for concrete tracks (System 300-1). In the concrete tracks, the elastic rail fastening system sticks the rail and the sleeper and reacts all the time when the train is running. Among the components of the rail fastening system, the resilience pad and tension clamp were fatigue members and were constantly deformed in response to compressive and uplift forces. In this study, the residual deformation characteristics of the tension clamp according to the period of use were analyzed using by specimens taken on site in the same section for 6, 11, and 16 years on the serviced urban transit. In addition, the damage mechanisms for each component were derived based on finite element analysis. As a result of the numerical analysis, the stress (strain) of each part of the tension clamp according to the external force from the applied clamping force was analyzed to derive the damaged weak part of the tension clamp.

• Journal of the Earthquake Engineering Society of Korea
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• v.27 no.2
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• pp.77-82
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• 2023

The structural analysis module is an essential part of any integrated structural system. Diverse integrated systems today require, from the analysis module, efficient real-time responses to real-time input such as earthquake signals, extreme weather-related forces, and man-made accidents. An integrated system may also be for the entire life span of a civil structure conceived during the initial conception, developed throughout various design stages, effectively used in construction, and utilized during usage and maintenance. All these integrated systems' essential part is the structural analysis module, which must be automated and computationally efficient so that responses may be almost immediate. The finite element method is often used for structural analysis, and for automation, many effective finite element meshes must be automatically generated for a given analysis. A computationally efficient finite element mesh generation scheme based on the r-h method of mesh refinement using strain deviations from the values at the Gauss points as error estimates from the previous mesh is described. Shape factors are used to sort out overly distorted elements. A standard cantilever beam analyzed by four-node plane stress elements is used as an example to show the effectiveness of the automated algorithm for a time-domain dynamic analysis. Although recent developments in computer hardware and software have made many new applications in integrated structural systems possible, structural analysis still needs to be executed efficiently in real-time. The algorithm applies to diverse integrated systems, including nonlinear analyses and general dynamic problems in earthquake engineering.

• Computers and Concrete
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• v.31 no.5
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• pp.419-432
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• 2023

Beam-column joints are a critical component of reinforced concrete frame structures. They are responsible for transferring forces between adjoining beams and columns while limiting story drifts and maintaining structural integrity. During severe loading, beam-column joints deform significantly, affecting, and sometimes governing, the overall response of frame structures. While most failure modes for beam and column elements are commonly considered in plastic-hinge-based global frame analyses, the beam-column joint failure modes, such as concrete shear and reinforcement bond slip, are frequently omitted. One reason for this is the dearth of published guidance on what type of hinges to use, how to derive the joint hinge properties, and where to place these hinges. Many beam-column joint models are available in literature but their adoption by practicing structural engineers has been limited due to their complex nature and lack of practical application tools. The objective of this study is to provide a comparative review of the available beam-column joint models and present a practical joint modeling approach for integration into commonly used global frame analysis software. The presented modeling approach uses rotational spring models and is capable of modeling both interior and exterior joints with or without transverse reinforcement. A spreadsheet tool is also developed to execute the mathematical calculations and derive the shear stress-strain and moment-rotation curves ready for inputting into the global frame analysis. The application of the approach is presented by modeling a beam column joint specimen which was tested experimentally. Important modeling considerations are also presented to assist practitioners in properly modeling beam-column joints in frame analyses.

• Advances in nano research
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• v.16 no.6
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• pp.623-638
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• 2024

This study investigates the thermal post-buckling behavior of concrete eccentric annular sector plates reinforced with graphene oxide powders (GOPs). Employing the minimum total potential energy principle, the plates' stability and response under thermal loads are analyzed. The Haber-Schaim foundation model is utilized to account for the support conditions, while the transform differential quadrature method (TDQM) is applied to solve the governing differential equations efficiently. The integration of GOPs significantly enhances the mechanical properties and stability of the plates, making them suitable for advanced engineering applications. Numerical results demonstrate the critical thermal loads and post-buckling paths, providing valuable insights into the design and optimization of such reinforced structures. This study presents a machine learning algorithm designed to predict complex engineering phenomena using datasets derived from presented mathematical modeling. By leveraging advanced data analytics and machine learning techniques, the algorithm effectively captures and learns intricate patterns from the mathematical models, providing accurate and efficient predictions. The methodology involves generating comprehensive datasets from mathematical simulations, which are then used to train the machine learning model. The trained model is capable of predicting various engineering outcomes, such as stress, strain, and thermal responses, with high precision. This approach significantly reduces the computational time and resources required for traditional simulations, enabling rapid and reliable analysis. This comprehensive approach offers a robust framework for predicting the thermal post-buckling behavior of reinforced concrete plates, contributing to the development of resilient and efficient structural components in civil engineering.

• Steel and Composite Structures
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• v.51 no.3
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• pp.243-260
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• 2024

Thirteen self-compacting recycled concrete filled aluminium tubular (SCRCFAT) columns were tested under concentric compression loads. The effects of the replacement ratio of the recycled concrete aggregate (RCA) and steel fibre (SF) reinforcement on the structural performance of the SCRCFAT columns were studied. A control specimen (C000) was cast with normal concrete without SF to be reference for comparison. Twelve columns were cast using RCA, six columns were cast using concrete incorporating 2% SF while the rest of columns were cast without SF. Failure mode, ductility, ultimate load capacity, axial deformation, ultimate strains, stress-strain response, and stiffness of the SCRCFAT columns were studied. The results showed that, the peak load of tested SCRCFAT columns incorporating 5-100 % RCA without SF reduced by 2.33-11.28 % compared to that of C000. Conversely, the peak load of tested SCRCFAT columns incorporating 5-100% RCA in addition to 2% SF increased by 21.1-40.25%, compared to C000. Consequently, the ultimate axial deformation (Δ) of column C100 (RCA=100% and SF 0%) increased by about 118.9 % compared to C000. The addition of 2% SF to the concrete mix decreased the axial deformation of SCRCFAT columns compared to those cast with 0% SF. Moreover, the stiffness of the columns cast without SF decreased as the RCA % increased. In contrast, the columns stiffness cast with 2% SF increased by 26.28-89.7 % over that of C000. Finally, a theoretical model was proposed to predict the ultimate loads tested SCRCFAT columns and the obtained theoretical results agreed well with the experimental results.

• Journal of the Korea institute for structural maintenance and inspection
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• v.26 no.6
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• pp.167-174
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• 2022

The objective of this study is to evaluate the dynamic properties of DUS (damping-up system) composed of the materials with excellent damping capacity, and to compare with those of the conventional hangar bolt. The main parameters are the type and hardness (𝜂_H), of rubber and the prestress force (value converted from the compression strain (𝜂_R) in the stress-strain relationship of rubber). The dynamic properties were examined from the natural frequency (𝜔_n), maximum response acceleration (A_m), amplification coefficient (𝛼_p), maximum relative displacement (𝚫_m), and damping ratio (𝜉_D). The test results showed that the A_m, 𝛼_p, and 𝚫_m values of DUS were 46.3%, 46.6% and 62.9% lower, respectively, and the 𝜉_D value was 3.89 times higher, when compared to those of the conventional hangar bolt. In particular, the 𝛼_p value was 1.3 for DUS, and 2.45 for the conventional hanger bolt, which were similar to those of rigid and flexible components specified in KDS 41 17 00, respectively. Consequently, in the optimal details of DUS, the 𝜂_H values of 50 and 45 were required for the NR (natural rubber) and EPDM (ethylene propylene diene monomer), and the 𝜂_R value of 5% was also recommended.

• Korean Journal of Microbiology
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• v.41 no.2
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• pp.99-104
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• 2005

Corynebacterial clones which exert regulatory effects on the expression of the glyoxylate bypass genes were isolated using a reporter plasmid carrying the enteric lacZ fused to the aceB promoter of Corynebacterium glutamicum. Some clones carried common fragments as turned out by DNA mapping technique. Subcloning analysis followed by the measurement of $\beta-galactosidase$ activity in Escherichia coli identified the region responsible for the aceB-repressing activity. Sequence analysis of the DNA fragment identified two independent ORFs of ORF1 and ORF2. Among them, ORF2 was turned out to be responsible for the aceB-repressing activity. ORF1 encoded a 23,216 Da protein composed of 206 amino acids. Sequence similarity search indicated that the ORF may encode a ECF-type $\sigma$ factor and designated sigH. To identify the function of sigH, C. glutamicum sigH mutant was constructed by gene disruption technique and the sigH mutant showed growth retardation as compared to the wild type strain. In addition, the mutant strain showed sensitivity to oxidative-stress generating agent plumbagin. This result imply that sigH is probably involved in the stress response occurring during normal cell growth.

• Journal of the Korea Concrete Institute
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• v.28 no.5
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• pp.535-544
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• 2016

This paper aims at examining the effects of sustained load and elevated temperature on the time-dependent deformation of a carbon fiber reinforced polymer (CFRP) sheets bonded to concrete as well as the pull-off strength of single-lap shear specimens after the sustained loading period using digital images. Elevated temperature during the sustained loading period resulted in increased slip of the CFRP composites, whereas increased curing time of the polymer resin prior to the sustained loading period resulted in reduced slip. Pull-off tests conducted after sustained loading period showed that the presence of sustained load resulted in increased pull-off strength and interfacial fracture energy. This beneficial effect decreased with increased creep duration. Based on analysis of digital images, results on strain distributions and fracture surfaces indicated that stress relaxation of the epoxy occurred in the 30 mm closest to the loaded end of the CFRP composites during sustained loading, which increased the pull-off strength provided the failure locus remained mostly in the concrete. For longer sustained loading duration, the failure mode of concrete-CFRP bond region can change from a cohesive failure in the concrete to an interfacial failure along the concrete/epoxy interface, which diminished part of the strength increase due to the stress relaxation of the adhesive.

• Journal of Life Science
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• v.31 no.1
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• pp.28-36
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• 2021

Edwardsiella piscicida is a significant cause of hemorrhagic septicemia in fish and gastrointestinal infections in humans. Survival bacteria require specialized mechanisms to adapt to environmental fluctuations. Hence, to understand the mechanism through which E. piscicida senses and responds to environmental osmolarity changes, we determined the protein expression profile and physiological properties under various salinity conditions in this study. The OmpR protein is a part of the Env-ZOmpR two-component system that has been implicated in sensing salt stress in bacteria. However, the physiological role played by this protein in E. piscicida remains to be elucidated. Therefore, in this work, the function of the OmpR protein in response to salt stress was investigated. Phenotypic analysis revealed that, in the mutant, three of the biochemical phenotypes were different from the wild type, including, citrate utilization, hydrogen sulfide, and indole production. Introduction of the plasmid containing the entire ompR gene to the mutant strain returned it to its parental phenotype. The retarded growth rate also partially recovered. Furthermore, in our studies, OmpR was not found to be related to cell motility. Taken together, our results from the mutational analysis, the growth assay, MALDI-TOF MS, qRT-PCR, and the phenotype studies suggest that the OmpR of E. piscicida is implicated in osmoregulation, growth, expression of porins (ETAE_1826), virulence-related genes (EseC, EseD and EvpC), and certain genes of unknown function (ETAE_1540 and ETAE_2706).