Purpose : Professional self-concept of nurse is a critical factor that strengthens confidence and a sense of professionalism in practice, significantly influencing nurses' intention to stay in their jobs or leave. Establishing a strong professional self-concept during nursing education is essential for shaping a positive professional identity and role. Therefore, this study aimed to examine the level of clinical practice stress, resilience and professional self-concept among third-year nursing students who have completed their first clinical practice, as well as to analyze the relationships among these variables and identify factors influencing professional self-concept. Methods : The study was conducted with 237 third-year nursing students from two nursing departments in H and S cities, all of whom consented to participate in the survey. A structured online questionnaire was used to assess the students' clinical practice stress, resilience, and professional self-concept. Data collection was carried out over a period of approximately three months, from September to November 2023. Data analysis was performed using SPSS 26.0, applying descriptive statistics, independent t-tests, ANOVA, scheffe's test, pearson correlation, and hierarchical regression analysis. Results : Professional self-concept showed a negative correlation with clinical practice stress (r=-0.38, p<.001) and a positive correlation with resilience (r=0.52, p<.001), both of which were statistically significant. The significant predictors of professional self-concept were clinical practice stress (𝛽=-0.45, p=.010), resilience (𝛽=0.36, p<.001), admission motivation*score (𝛽=-0.19, p=.010), major satisfaction*moderate (𝛽=-0.18, p=.004), relationships with friends*moderate (𝛽=-0.13, p=.013), and academic achievement*grade B (𝛽=-0.13, p= .013). These factors explained 45.00 % of the variance in professional self-concept, with clinical practice stress serving as a key predictor. Conclusion : A professional self-concept enhancement program, tailored to individual characteristics, should be developed and implemented from the freshman year to improve nursing students' resilience. This would help reduce clinical practice stress during the clinical performance training phase and establish a positive professional self-concept among nursing students.