• 한국초지조사료학회지
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• 제38권3호
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• pp.190-195
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• 2018

Cold, salt and heat are the most critical factors that restrict full genetic potential, growth and development of crops globally. However, clarification of genes expression and regulation is a fundamental approach to understanding the adaptive response of plants under unfavorable environments. In this study, we applied an annealing control primer (ACP) based on the GeneFishing approach to identify differentially expressed genes (DEGs) in Italian ryegrass (cv. Kowinearly) leaves under cold, salt and heat stresses. Two-week-old seedlings were exposed to cold ($4^{\circ}C$), salt (NaCl 200 mM) and heat ($42^{\circ}C$) treatments for six hours. A total 8 differentially expressed genes were isolated from ryegrass leaves. These genes were sequenced then identified and validated using the National Center for Biotechnology Information (NCBI) database. We identified several promising genes encoding light harvesting chlorophyll a/b binding protein, alpha-glactosidase b, chromosome 3B, elongation factor 1-alpha, FLbaf106f03, Lolium multiflorum plastid, complete genome, translation initiation factor SUI1, and glyceraldehyde-3-phosphate dehydrogenase. These genes were potentially involved in photosynthesis, plant development, protein synthesis and abiotic stress tolerance in plants. However, this study provides new insight regarding molecular information about several genes in response to multiple abiotic stresses. Additionally, these genes may be useful for enhancement of abiotic stress tolerance in fodder crops as well a crop improvement under unfavorable environmental conditions.

• Molecules and Cells
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• 제26권6호
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• pp.595-605
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• 2008

Genome wide transcription analysis in response to stresses is essential to provide the basis of effective engineering strategies to improve stress tolerance in crop plants. In order to perform transcriptome analysis in Brassica rapa, we constructed a B. rapa oligo microarray, KBGP-24K, using sequence information from approximately 24,000 unigenes and analyzed cold ($4^{\circ}C$), salt (250 mM NaCl), and drought (air-dry) treated B. rapa plants. Among the B. rapa unigenes represented on the microarray, 417 (1.7%), 202 (0.8%), and 738 (3.1%) were identified as responsive genes that were differently expressed 5-fold or more at least once during a 48-h treatment with cold, salt, and drought, respectively. These results were confirmed by RT-PCR analysis. In the abiotic stress responsive genes identified, we found 56 transcription factor genes and 60 commonly responsive genes. It suggests that various transcriptional regulatory mechanisms and common signaling pathway are working together under the abiotic stresses in B. rapa. In conclusion, our new developed 24K oligo microarray will be a useful tool for transcriptome profiling and this work will provide valuable insight in the response to abiotic stress in B. rapa.

• The Plant Pathology Journal
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• 제26권2호
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• pp.110-114
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• 2010

WRKY transcription factors are known to be involved in many different biological processes including plant response to biotic stress, abiotic stress, and plant development. WRKY proteins are extensively studied in Arabidopsis. Recently, reports on WRKY proteins are rapidly increasing in the other plant species, especially in rice. Therefore, this review will discuss the function of rice WRKY proteins reported so far.

• 지능정보연구
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• 제14권2호
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• pp.21-44
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• 2008

최근 유비쿼터스 컴퓨팅 환경에서 등장하고 있는 각종 서비스들은 공통적으로 상황인식 컴퓨팅(context-aware computing)의 속성을 갖고 있다. 사용자를 둘러싼 다양한 상황(context)을 인식하여 다른 곳으로 정보를 전송하거나 지능적인(intelligent) 서비스를 제공해주는 것을 유비쿼터스 컴퓨팅 서비스의 기본 속성이라고 할 때, 유비쿼터스 컴퓨팅 서비스는 웰빙케어(well-being care) 분야에도 적용될 수 있다. 본 연구는 상황인식 컴퓨팅의 속성을 스트레스 관리를 위한 새로운 서비스에 적용해 봄으로써, 스트레스 정도를 객관적으로 측정할 수 있고 스트레스를 줄여주는 개인화 된 항스트레스(personalized anti-stress) 서비스를 제공할 수 있는 방법을 고안하였다. 이를 위해 본 연구는 스트레스 지수 모델(stress index model)을 개발하고, 스트레스를 측정하여 그에 맞는 항스트레스 서비스를 제공할 수 있는 방법론적인 접근을 시도하였다. 또한, 데모시스템의 테스트를 통해 스트레스 지수 모델의 기술적 구현 가능성을 확인하였다. 뿐만 아니라, 이와 같은 방법들을 사업적 관점에서 실제 적용할 수 있는 방법의 하나로서, 스트레스 측정과 관리를 주 목적으로 하는 u-SMC(Stress Management Center)의 비즈니스 모델을 개발함으로써 스트레스 관리를 위한 유비쿼터스 컴퓨팅 서비스의 상용화 가능성도 살펴보았다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.25-25
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• 2017

Inflorescence architecture mainly contributes to final grain yield in crops. Sorghum inflorescence is basically composed of one fertile sessile spikelet (SS) and two infertile pedicellate spikelets (PS). To identify regulatory factors involved in the inflorescence architecture, we screened an EMS mutagenesis population from the pedigreed sorghum mutant library. We found inflorescent architecture mutants, named as multi-seed mutants, msd, with gained fertile ability in PS and also an increased number of floral branches. In natural sorghum populations, it is not common that are fertile. A detailed dissection of developmental stages of wild type and msd1 mutant described that the PS in wild type do not have floral organs, including ovary, stigma, filament and anther, while the msd1 mutants generate intact floral organ in the sessile spikelet. We found MSD1 encoded a TCP transcription factor using bulk segregant analysis (BSA) of F2 population, and was a strongly enriched expression during inflorescence developmental stages. We proposed that MSD1 functions to suppress floral organ maintenance at PS during inflorescence development in Sorghum. To explore the regulatory network associated with PS fertility, whole genome expression profiling was performed at 4 different developmental stages in 6 various tissue types between wild type and msd1. Taken together, we demonstrated that MSD1 was involved in the plant hormone and maybe influenced program cell death in PS via the activation of plant hormonal pathway.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2008년도 춘계 학술발표회 초청강연 및 논문집
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• pp.754-761
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• 2008

In general, stress measurement of existent shotcrete lining be used by pressure cells. but, measuring instrument is lost by high pressure at shotcrete lining construction and pressure cell's measurement value have to low believability by natural conditions like curing temperature. In this study, proposed techniques to measure without utilizing sensitive stress sensor in natural condition at point that want stress of shotcrete lining after shotcrete lining construction. Executed numerical analysis to forecast stress level that interact in tunnel shotcrete lining, measured strain of hole by load action through hole in shotcrete lining. 3D FEM(finite element method) is enforced through various parameters curing time of shotcrete lining, thickness, load condition. Different model cases applied by parametic study. As analysis result, it could grasp development possibility of method that propose this time because it could examine corelation with strain by near hole of shotcrete lining and stress about load condition.

• 가족자원경영과 정책
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• 제19권1호
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• pp.1-21
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• 2015

Unlike daily hassles or minor stressors, immigration is an intense life event that may cause excessive stress. In particular, immigrant parents find themselves in a more stressful situation in that they experience stresses caused by immigration and stresses caused by parenting at the same time. The purpose of this study is to develop and validate an Immigrant Parental Stress Inventory (IPSI), that objectively and comprehensively assesses the stress experienced by immigrant parents within the Korean context. Based on the Immigrant Parental Stress Inventory developed within the American context, preliminary items were refined through a literature review and the discussions of experts and immigrant parents and were empirically validated with a sample of 203 immigrant parents residing in Korea. Through exploratory and confirmatory factor analysis, six main factors were identified (Factor1 Difficulties in communication, Factor2 Financial difficulties, Factor3 Difficulties in social adjustment, Factor4 Social discrimination, Factor5 Lack of social resources, Factor6 Difficulties in parenting). The IPSI was shown to be a psychometrically sound instrument that is capable of measuring immigrant parental stress within the Korean context. Social service professionals and researchers studying or working with immigrant families could use the IPSI to examine immigrant parental stress.

• 농업과학연구
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• 제50권4호
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• pp.883-902
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• 2023

Water is critical to the health and productivity of fruit trees. Efficient monitoring of water stress is essential for optimizing irrigation practices and ensuring sustainable fruit production. Short-range sensing can be reliable, rapid, inexpensive, and used for applications based on well-developed and validated algorithms. This paper reviews the recent advancement in fruit tree water stress detection via short-range sensing, which can be used for irrigation scheduling in orchards. Thermal imagery, near-infrared, and shortwave infrared methods are widely used for crop water stress detection. This review also presents research demonstrating the efficacy of short-range sensing in detecting water stress indicators in different fruit tree species. These indicators include changes in leaf temperature, stomatal conductance, chlorophyll content, and canopy reflectance. Short-range sensing enables precision irrigation strategies by utilizing real-time data to customize water applications for individual fruit trees or specific orchard areas. This approach leads to benefits, such as water conservation, optimized resource utilization, and improved fruit quality and yield. Short-range sensing shows great promise for potentially changing water stress monitoring in fruit trees. It could become a useful tool for effective fruit tree water stress management through continued research and development.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제35권3호
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• pp.163-168
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• 2024

Stress is a natural state that emerges due to the dynamics of an individual's life. Children must learn how to effectively manage stress as part of their growth and development. Resolution is possible when children are exposed to stress and receive adequate support from their families. However, when stress is intense, frequent, protracted, or traumatic, as in cases of childhood abuse, it can become toxic and interfere with the development of the child's brain and body. This results in vulnerability, which can have detrimental effects on the child's overall physical, mental, and emotional health. This perspective discusses the impact of childhood maltreatment and toxic stress, drawing on insights gained during the COVID-19 pandemic. We aimed to shed light on the lessons learned from this unique and challenging period and how they inform our understanding of the effects of stress on children's well-being.

• 아동학회지
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• 제34권6호
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• pp.77-96
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• 2013

The purpose of this study was to examine the relationships among mother's psychological characteristics(self-esteem, self-efficacy, and depression), parenting related factors(maternal parenting stress and mother's knowledge on infant development), maternal parenting style, and two-year-old infants' development. The participants consisted of 1735 mothers and their two-year old infants selected from a sample of the 3rd Panel Study of Korean Children(PSKC) by the Korea Institute of Child Care and Education(KICCE), conducted in 2010. Data were analyzed by means of a Structural Equation Model(SEM) to identify the structure and relationships among the various factors. The significance of direct and indirect effects to test the mediating effect of maternal parenting style were identified via bootstrapping. The results indicated that maternal parenting style most definitely mediated the relationship among the mother's psychological characteristics(self-esteem and self-efficacy), parenting related factors(maternal parenting stress and mother's knowledge on Infant development) and the development of their two-year-old infants.