Browse > Article
http://dx.doi.org/10.5333/KGFS.2018.38.3.190

Screening of Multiple Abiotic Stress-Induced Genes in Italian Ryegrass leaves  

Lee, Sang-Hoon (Animal Genetic Resources Center, National Institute of Animal Science, Rural Development Administration)
Rahman, Md. Atikur (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Kim, Kwan-Woo (Animal Genetic Resources Center, National Institute of Animal Science, Rural Development Administration)
Lee, Jin-Wook (Animal Genetic Resources Center, National Institute of Animal Science, Rural Development Administration)
Ji, Hee Chung (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Choi, Gi Jun (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Song, Yowook (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Lee, Ki-Won (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.38, no.3, 2018 , pp. 190-195 More about this Journal
Abstract
Cold, salt and heat are the most critical factors that restrict full genetic potential, growth and development of crops globally. However, clarification of genes expression and regulation is a fundamental approach to understanding the adaptive response of plants under unfavorable environments. In this study, we applied an annealing control primer (ACP) based on the GeneFishing approach to identify differentially expressed genes (DEGs) in Italian ryegrass (cv. Kowinearly) leaves under cold, salt and heat stresses. Two-week-old seedlings were exposed to cold ($4^{\circ}C$), salt (NaCl 200 mM) and heat ($42^{\circ}C$) treatments for six hours. A total 8 differentially expressed genes were isolated from ryegrass leaves. These genes were sequenced then identified and validated using the National Center for Biotechnology Information (NCBI) database. We identified several promising genes encoding light harvesting chlorophyll a/b binding protein, alpha-glactosidase b, chromosome 3B, elongation factor 1-alpha, FLbaf106f03, Lolium multiflorum plastid, complete genome, translation initiation factor SUI1, and glyceraldehyde-3-phosphate dehydrogenase. These genes were potentially involved in photosynthesis, plant development, protein synthesis and abiotic stress tolerance in plants. However, this study provides new insight regarding molecular information about several genes in response to multiple abiotic stresses. Additionally, these genes may be useful for enhancement of abiotic stress tolerance in fodder crops as well a crop improvement under unfavorable environmental conditions.
Keywords
Abiotic stress; Forage; Gene; Italian Ryegrass;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Rahman, M.A., Lee, S.H., Kim, K.Y., Park, H.S., Hwang, T.Y., Choi, G.J. and Lee, K.W. 2016b. Arsenic-induced differentially expressed genes identified in Medicago sativa L. roots. Journal of The Korean Society of Grassland and Forage Science. 36:243-247.   DOI
2 Sahu, B.B. and Shaw, B.P. 2009. Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte,using PCR-based suppression subtractive hybridization. BMC Plant Biology. 9:69-69.   DOI
3 Singer, S.D., Hannoufa, A. and Acharya, S. 2017. Molecular improvement of alfalfa for enhanced productivity and adaptability in a changing environment. Plant, cell & environment. 13090.
4 Staniak, M. and Kocon, A. 2015. Forage grasses under drought stress in conditions of Poland. Acta Physiologiae Plantarum. 37:116.   DOI
5 Tuteja, N., Gill, S.S. and Tuteja, R. 2011. Plant responses to abiotic stresses: shedding light on salt, drought, cold and heavy metal stress. Omics and Plant Abiotic Stress Tolerance. pp. 39-64.
6 Velculescu, V.E., Zhang, L., Vogelstein, B. and Kinzler, K.W. 1995. Serial analysis of gene expression. Science. 270:484-487.   DOI
7 Wang, D., Heckathorn, S.A., Mainali, K. and Tripathee, R. 2016. Timing effects of heat-stress on plant ecophysiological characteristics and growth. Frontiers in Plant Science. 7.
8 Xie, W., Zhang, J., Zhao, X., Zhang, Z. and Wang, Y. 2017. Transcriptome profiling of Elymus sibiricus, an important forage grass in Qinghai-Tibet plateau, reveals novel insights into candidate genes that potentially connected to seed shattering. BMC Plant Biology. 17:78.   DOI
9 Li, Y., Wan, L., Bi, S., Wan, X., Li, Z., Cao, J., Tong, Z., Xu, H., He, F. and Li, X. 2017. Identification of drought-responsive micrornas from roots and leaves of alfalfa by high-throughput sequencing. Genes. 8:119.   DOI
10 Lou, Y., Zhao, P., Wang, D., Amombo, E., Sun, X., Wang, H. and Zhuge, Y. 2017. Germination, physiological responses and gene expression of tall fescue (Festuca arundinacea Schreb.) growing under Pb and Cd. PLOS ONE. 12:e0169495.   DOI
11 Paredes, M. and Quiles, M.J. 2015. The effects of cold stress on photosynthesis in Hibiscus plants. PLOS ONE. 10:e0137472.   DOI
12 Pavinato, P.S., Restelatto, R., Sartor, L.R. and Paris, W. 2014. Production and nutritive value of ryegrass (cv. Barjumbo) under nitrogen fertilization. Revista Ciencia Agronomica. 45:230-237.   DOI
13 Pennycooke, J.C., Jones, M.L. and Stushno, V.C. 2003. Down-regulating ${\alpha}$-galactosidase enhances freezing tolerance in transgenic petunia. Plant Physiology. 133:1-9.   DOI
14 Pokalsky, A., Hiatt, W., Ridge, N., Rasmussen, R., Houck, C. and Shewmaker, C. 1989. Structure and expression of elongation factor $1{\alpha}$ in tomato. Nucleic Acids Research. 17:4661-4673.   DOI
15 Qin, D., Dong, J., Xu, F., Ge, S., Xu, Q. and Li, M. 2017. Genome-wide identification and characterization of light harvesting chlorophyll a/b binding protein genes in barley (Hordeum vulgare L.) Advances in Crop Science and Technology. 301.
16 Rahman, M.A., Alam, I., Kim, Y.G., Ahn, N.Y., Heo, S.H., Lee, D.G., Liu, G. and Lee, B.H. 2015. Screening for salt-responsive proteins in two contrasting alfalfa cultivars using a comparative proteome approach. Plant Physiology and Biochemistry. 89:112-122.   DOI
17 Kopecky, D. and Studer, B. 2014. Emerging technologies advancing forage and turf grass genomics. Biotechnology Advances. 32:190-199.   DOI
18 Rahman, M.A., Kim Y.G., Alam, I., Liu, G., Lee, H., Lee, J.J. and Lee, B.H. 2016a. Proteome analysis of alfalfa roots in response to water deficit stress. Journal of Integrative Agriculture. 15:1275-1285.   DOI
19 Hulke, B.S., Watkins, E., Wyse, D.L. and Ehlke, N.J. 2008. Freezing tolerance of selected perennial ryegrass (Lolium perenne L.) accessions and its association with field winter hardiness and turf traits. Euphytica. 163:131-141.   DOI
20 Jain, M. 2012. Next-generation sequencing technologies for gene expression profiling in plants. Briefings in Functional Genomics. 11:63-70.   DOI
21 Lee, K.W., Rahman, M.A., Choi, G.J., Ji, H.C., Hwang, T.Y. and Lee, S.H. 2018. Identification of differentially expressed abiotic stress-induced genes in teff grass (Eragrostis tef) leaves. The Journal of Animal and Plant Science. 28:1189-1193.
22 Lee, K.W., Choi, G. J., Kim, K.Y., Ji, H.C., Zaman, R. and Lee, S.H. 2011. Identification of drought induced differentially expressed genes in barley leaves using annealing control-primer-based Gene Fishing technique. Australian Journal of Crop Science. 5:1364-1369.
23 Alam, I., Kim, K.H., Sharmin, S.A., Kim, Y.G., and Lee, B.H. 2010. Advances in the molecular breeding of forage crops for abiotic stress tolerance. Journal of Plant Biotechnology. 37:425-441.   DOI
24 Azevedo, H., Azinheiro, S.G., Munoz-Merida, A., Castro, P.H., Huettel, B., Aarts, M.G.M. and Assuncao, A.G.L. 2016. Transcriptomic profiling of Arabidopsis gene expression in response to varying micronutrient zinc supply. Genomics Data. 7:256-258.   DOI
25 Chalanika De Silva, H.C. and Asaeda, T. 2017. Effects of heat stress on growth, photosynthetic pigments, oxidative damage and competitive capacity of three submerged macrophytes. Journal of Plant Interactions. 12:228-236.   DOI
26 Lee, S.H., Lee K.W., Kim, K.Y., Choi, G.J., Yoon, S.Y., Ji, H.C., Seo, S., Lim, Y.C. and Ahsan, N. 2009. Identification of salt-stress induced differentially expressed genes in barley leaves using the annealing-control-primer-based GeneFishing technique. African Journal of Biotechnology. 8:1326-1331.
27 Chrost, B., Kolukisaoglu, U., Schulz, B. and Krupinska, K. 2007. An ${\alpha}$-galactosidase with an essential function during leaf development. Planta. 225:311-320.
28 Brenner, S., Johnson, M., Bridgham, J., Golda, G., Lloyd, D.H., Johnson, D., Luo, S., McCurdy, S., Foy, M., Ewan, M., Roth, R., George, D., Eletr, S., Albrecht, G., Vermaas, E., Williams, S.R., Moon, K., Burcham, T., Pallas, M., DuBridge, R.B., Kirchner, J., Fearon, K., Mao, J.-I. and Corcoran, K. 2000. Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays. Nature Biotechnology. 18:630.   DOI
29 Choulet, F., Alberti, A., Theil, S., Glover, N., Barbe, V., Daron, J., Pingault, L., Sourdille, P., Couloux, A., Paux, E., Leroy, P., Mangenot, S., Guilhot, N., Le Gouis, J., Balfourier, F., Alaux, M., Jamilloux, V., Poulain, J., Durand, C., Bellec, A., Gaspin, C., Safar, J., Dolezel, J., Rogers, J., Vandepoele, K., Aury, J.-M., Mayer, K., Berges, H., Quesneville, H., Wincker, P. and Feuillet, C. 2014. Structural and functional partitioning of bread wheat chromosome 3B. Science. 345.
30 Gerdes, L., Mattos, H.B.d., Werner, J.C., Colozza, M.T., Cunha, E.A.d., Bueno, M.S., Possenti, R.A. and Schammass, E.A. 2005. Chemical composition and digestibility of forage mass in irrigated aruanagrass pastures or oversown with a mixture of winter forage species. Revista Brasileira de Zootecnia. 34:1098-1108.   DOI
31 Zeng, L., Deng, R., Guo, Z., Yang, S. and Deng, X. 2016. Genome-wide identification and characterization of Glyceraldehyde-3-phosphate dehydrogenase genes family in wheat (Triticum aestivum). BMC Genomics. 17:240.   DOI
32 Yin, H., Gao, P., Liu, C., Yang, J., Liu, Z. and Luo, D. 2013. SUI-family genes encode phosphatidylserine synthases and regulate stem development in rice. Planta. 237:15-27.   DOI