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Attenuation of Atherosclerosis by 3,4-Dihydroxy-Hydrocinnamic Acid in Rabbits by Partial Inhibition of ACAT (토끼에서 ACAT 억제에 의한 3,4-다이하이드록시 하이드로시나믹산의 동맥경화 완화 효과)

  • Lee, Mi-Ran;Choi, Jae-Hoon;Yang, Young;Oh, Ki Sook;Jeong, Tae-Sook;Lee, Chul-Ho;Oh, Goo Taeg
    • Korean Journal of Clinical Laboratory Science
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    • v.48 no.4
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    • pp.280-286
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    • 2016
  • Polyphenols have been reported to have beneficial effects on cardiovascular disease. A polyphenolic compound, 3,4-dihydroxy-hydrocinnamic acid (3,4-DHHCA), has been shown to have antioxidative and antitumorigenic activities. However, the effect of 3,4-DHHCA on atherosclerosis is still unknown. Herein, we investigated the effects of 3,4-DHHCA on atherosclerosis in New Zealand White rabbits. Broad and fused fatty streak lesions were found in rabbits fed with high-cholesterol diet for 8 weeks. Administration of 3,4-DHHCA reduced atherosclerotic lesion formation and lesional accumulation of macrophage in rabbits fed with cholesterol diet without systemic or local toxicity. Hepatic acyl-coenzyme A: cholesterol acyltransferase (ACAT) activity was decreased after treatment with 3,4-DHHCA by 22% in cholesterol diet-fed rabbits compared with the control group. These results indicate that 3,4-DHHCA had antiatherogenic effects in rabbits, possibly by partial inhibition of ACAT.

An analysis of Characteristics of Heavy Rainfall Events over Yeongdong Region Associated with Tropopause Folding (대류권계면 접힘에 의한 영동지방 집중호우사례의 특성분석)

  • Lee, Hye-Young;Ko, Hye-Young;Kim, Kyung-Eak;Yoon, Ill-Hee
    • Journal of the Korean earth science society
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    • v.31 no.4
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    • pp.354-369
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    • 2010
  • The synoptic and kinematic characteristics of a heavy rainfall that occurred in Gangneung region on 22 to 24 October 2006 were investigated using weather maps, infrared images, AWS observation data and NCEP global final analyses data. The total amount of rainfall observed in the region for the period was 316.5 mm, and the instanteneous maximum wind speed was $63.7m\;s^{-1}$. According to the analysis of weather maps, before the starting of the heavy rainfall, an extratropical low pressure system was developed in the middle region of the Korean Peninsula, and an inverted trough was formed in the northern region of the peninsula. In addition, a jet stream on the upper charts of 300 hPa was located over the Yellow Sea and the southern boundary of the peninsula. A cutoff low in the cyclonic shear side of the upper jet streak, which was linked to an anomaly of isentropic potential vorticity, was developed over the northwestern part of the peninsula. And there are analyzed potential vorticity and wind, time-height cross section of potential vorticity, vertical air motion, maximums of the divergence and convergence and vertical distribution of potential temperature in Gangneung region. The analyzed results of the synoptic conditions and kinematic processes strongly suggest that the tropopause folding made a significant role of initializing the heavy rainfall.

The Classifying Ability of the Igneous Rocks with Naked Eyes for Preservice Science Teachers (예비과학교사들의 화성암 육안분류 능력)

  • Moon Byoung Chan;Jeong Jin-Woo;Chung Chull Hwan
    • Journal of the Korean earth science society
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    • v.26 no.7
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    • pp.630-639
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    • 2005
  • The purpose of this study was to investigate the classifying ability of the igneous rocks with the naked eye for 36 preservice science teachers. For this, we selected six specimens of igneous rocks that consisted of rhyolite, andesite, basalt, granite, diorite, and gabbro, and performed the questionnaire with them. Preservice science teachers needed the average of 3 tools to classify the rocks. Most of the selected tools were loupe, streak plate, hammer and Mohs’ hardness scale. Many preservice science teachers selected basalt and granite samples to classify igneous rocks among 6 kinds of the rocks which were exhibited. However, the results of the identification with the naked eye showed that the right answer rate was significantly different based on what rock sample had been selected. Nobody gave the right answer among 10 students who chose the rhyolite sample, but all of 36 students who picked the basalt sample answered correctly. And $62\%$ of 8 students who chose the andesite sample, 62% of 32 student choosing granite, $7\%$ of 13 students choosing diorite and $44\%$ of 9 students choosing gabbro were correctly answered. In identifying igneous rock samples with the naked eye, most subjects relied on vesicular texture to basalt, and they used textural, color and empirical characters to granite. But, some felt more or less difficulty to distinguish between intermediate and light colors and to recognize porphyry.

Occurrence of Fusarium Wilt Caused by Fusarium oxysporum f. sp. lactucae and Cultivar Susceptibility on Lettuce (Fusarium oxysporum f. sp. lactucae에 의한 상추 시들음병 발생 및 품종간 감수성 비교)

  • Kim, Jin-Young;Hong, Sun-Sung;Lee, Jin-Gu;Lee, Hyun-Ju;Lim, Jae-Wook;Kim, Jin-Won;Kim, Hong-Gi
    • Research in Plant Disease
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    • v.14 no.2
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    • pp.79-84
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    • 2008
  • A wilt disease on lettuce was observed in 2006 and 2007 in commercial plastic house at main production areas of lettuce in Icheon, Yongin and Goyang of Gyeonggi Province. The disease was characterized by the wilting of lower leaves, accompanied by stunting symptoms of the whole plants. Old affected stems showed the black streak in the vascular system. The pathogen, Fusarium oxysporum f. sp. lactucae was isolated from stems and roots of diseased plants. Isolated pathogen also produced the microconidia and macroconidia with chlamydospores on carnation leaf agar medium. The pathogen easily invaded and made many chlamydospores on the roots of lettuce and also made dark streaking through the vascular in the lettuce stems. The density of Fusarium sp. in the severely diseased field soil was more higher populations than that in the healthy and less diseased field soil. The minimum population of pathogen would be above $10^3$cfu/g soil to induce the Fusarium wilt on lettuce in plastic house. The results of pathogenicity test showed 'Sunpung' and 'Mipungpochap' was high susceptible to Fusarium pathogen isolates while some cultivar 'Mihongjeokchukmyeon' and 'Jinjachukmyeon' showed moderate resistance. Disease development for some lettuce was related to treated temperature, so the symptom was more severe above $25^{\circ}C$. Selection of appropriate lettuce cultivar and planting time should be related for the successful control of Fusarium wilt.

Molecular Identification and Sequence Analysis of Coat Protein Gene of Ornithogalum mosaic virus Isolated from Iris Plant

  • Yoon, Hye-In;Ryu, Ki-Hyun
    • The Plant Pathology Journal
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    • v.18 no.5
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    • pp.251-258
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    • 2002
  • A potyvirus was isolated from cultivated Iris plants showing leaf streak mosaic symptom. Reverse transcription and polymerase chain reaction (RT-PCR) product of 1 kb long which encoded partial nuclear inclusion B and N-terminal region of viral coat protein (CP) genes for potyviruses was successfully amplified with a set of potyvirus-specific degenerate primers with viral RNA samples from the infected leaves: The RT-PCR product was cloned into the plasmid vector and its nucleotide sequences were determined. The nucleotide sequence of a CDNA clone revealed that the virus was an isolate of Ornithogalum moseic virus (OrMV) based on BLAST search analysis and was denoted as OrMV Korean isolate (OrMV-Ky). To further characterize the CP gene of the virus, a pair of OrMV-specific primers was designed and used for amplification of the entire CP gene of OrMV-Kr, The virus was easily and reliably detected from virus-infected Iris leaves by using the RT-PCR with the set of virus-specific primers. The RT-PCR product of the CP gene of the virus was cloned and its sequences were determined from selected recombinant CDNA clones. Sequence analysis revealed that the CP of OrMV-Kr consisted of 762 nucleotides, which encoded 253 amino acid residues. The CP of OrMV-Ky has 94.1-98.0% amino acid sequence identities (20 amino acid alterations) with that of other three isolates of OrMV, Two NT rich potential N-glycosylation motif sequences, NCTS and NWTM, and a DAC triple box responsible for aphid transmission were conserved in CPs of all the strains of OrMV. The virus has 58.5-86.2% amino acid sequence identities with that of other 16 potyviruses, indicating OrMV to be a distinct species of the genus. OrMV-Ky was the most related with Pterostylia virus Yin the phylogenetic tree analysis of CP at the amino acid level. This is the first report on the occurrence of OrMV in Iris plants in Korea. Data in this study indicate that OrMV is found in cultivated Iris plants, and may have mixed infection of OrMV and Iris severe mosaic virus in Korea.

A Study on Tree-ring Dating and Speciation of Charcoal found in Pumiceous Deposit of the Quaternary Nari Caldera, Ulleung Island, Korea (신생대 제4기 울릉도 나리칼데라 부석층에서 산출된 탄화목의 연륜연대 및 수종식별 연구)

  • Im, Ji Hyeon;Choo, Chang Oh
    • Economic and Environmental Geology
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    • v.48 no.6
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    • pp.501-508
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    • 2015
  • The purpose of this study is to provide an inquiry into paleovegetation (paleoenvironment), eruption age and inferred emplacement temperature of pyroclastic deposit using charcoal occurred in the Quaternary Nari Caldera, Ulleung Island. In order to obtain the data, we carried out species identification and tree-ring analysis, radiocarbon dating for the charcoal. This sample was collected from pumiceous deposit in lowermost member(Member N-5) of the Nari Tephra Formation, which corresponds to the wood branch that has well preserved the tree-ring structure. Speciation and outermost tree-ring of wood reveal Picea spp. and $263+{\alpha}$ years. The calibrated ages from the center(pith) to the outermost tree-ring are $20,260{\pm}230$, $19,995{\pm}245$, and $19,975{\pm}265cal\;BP$, respectively, which are accordant with the tree-ring position. The youngest age, <19,710 cal BP would have implications for the eruptive age of Member N-5. Our results indicate that Picea spp. is the paleovegetation representing that Nari Caldera was under cold and wet climate conditions during the late Pleistocene. Based on the silky luster, brittleness, color, and streak of charcoal, etc., the lowest emplacement temperatures of pyroclastic flow are interpreted to have been at least as high as $350{\sim}500^{\circ}C$.

The Effects of Dietary Supplements Included Green Tea, Buckwheat and Grape Leaf Extract on Lipid Metabolism and on Regression of Fatty Streak Lesions in F1B Golden Syrian Hamsters Fed the Atherogenic Diet (녹차, 메밀 및 포도잎 추출물 첨가식이가 동맥경화유발식이를 급여한 F1B Golden Syrian 햄스터의 지질대사와 대동맥의 지방 축적에 미치는 영향)

  • Kim, Young-Eon;Kwon, Eun-Kyung;Oh, Se-Wook;Han, Dae-Seok;Kim, In-Ho;Lee, Chang-Ho;Lee, Ho-Joon
    • Korean Journal of Food Science and Technology
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    • v.37 no.6
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    • pp.962-969
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    • 2005
  • Effects of 5 and 10% dietary supplement tablets containing green tea, buckwheat, and grape leaf extracts on atherosclerosis, and plasma and hepatic lipid compositions in hamsters fed antherogenic diet [modified AIN-76A containing 10% coconut oil (w/w), 0.05% cholesterol] were examined. Compared to atherogenic diet only groups, addition of supplement to atherogenic diet-fed group significantly down-regulated triglyceride and total cholesterol levels in plasma and liver. F1B hamsters fed atherogenic diet had greater foam cell accumulation compared to control and those fed atherogenic diet with addition of supplement. Lipid metabolisms of plasma and liver increased with addition of supplement dose-dependently, whereas aortic lipid accumulation decreased. These results indicate tablets supplemented with green tea, buckwheat, and grape leaf extracts have potential to prevent cardiovascular diseases in golden Syrian hamsters.

Histological and Ultrastructural Differentiations of the Neuroepithelial Cells in the Mouse Embryo during Early Neurulation (마우스 초기 신경배형성과정에서 신경상피세포의 조직학적 및 미세구조적 분화)

  • Kim, Yul-A;Chung, Young-Wha
    • Applied Microscopy
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    • v.29 no.1
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    • pp.105-124
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    • 1999
  • Histological and ultrastructural differentiations of the neuroepithelial cells in the mouse embryo during neurulation were observed. The neural plates and grooves consisted of pseudostratified columnar epithelium in the embryonic day (ED) 8 embryo were developed. In the ED 9 embryo, the neural tube was developed in all body length of embryo except both the cephalic and caudal ends. Secondary neurulation was shown at the tail bud of the ED 10 embryo. In the ED 8 embryo, the primitive streak was shown in the posterior end of the embryonic disc. The neuroepithelium, notochord and mesenchyme were well differentiated in the cephalic and cervical portions. In the ED 9 and 10 embryos, the roof plates of neural tubes were constituted of the closing of the surface ectodermal cells in the hindbrain and the neuroepithelial cells in the spinal cord. The floor plate of neural tube were consisted of the low pseudostratified columnar epithelium. The spinal motor nerve fibers were initially differentiated in the ED 10 embryo. According to the electron density of the cell and the differentiation of tell organelles, the neuroepithelial cells in the ED 9 and 10 embryos were classified into three types: dark, intermediate and light types. All types in the ED 9 embryo were observed but the dark cell in the ED 10 embryo was not done. The free ribosomes and polysomes in all neuroepithelial cells were developed. The RER and lipid droplets in the dark cell and the Golgi complex in the intermediate and light cells were observed. Many microfilaments in the cytoplasmic processes of intermediate cell and the microfilaments and microtubules in the light cell processes were observed to be well differentiated.

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Computational Fluid Analysis for the Otter Boards - 3 . Efficiency Analysis of the Single Cambered Otter Boards for the Various Slot Position - (전개판에 대한 수직해법 - 3 . 슬롯에 따른 단순만곡형전개판의 성능분석 -)

  • 고관서
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.27 no.4
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    • pp.278-285
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    • 1991
  • The authors propose to use the slot system in order to improve of the efficiency for the cambered otter boards. The experiment is divided into 2 parts, one is the efficiency model test, and the other is the visualization model test. The hydrodynamic characteristics of the model otter boards were tested by efficiency model test to measure the shearing, drag force of the models and visualization test using hydrogen bubble method to observe the streak-line and time-line of flow around the models, and milk spout method to observe the separation zone in the wake behind the models. This study tested for 5 models such ad without slot, slot position 0.2C, 0.4C, 0.6C and 0.8C. The results obtained are as follows: \circled1 The maximum C sub(L) of model otter board with slot position 0.6C in attack angle 27$^{\circ}$ was the highest of all models, it's value was 1.59. \circled2 In general, the L/D ratio of the one slot otter boards were 16~28% higher than otter board without slot. \circled3 The slot position 0.6C was better than any other slot position, and it's conformed by visiualization. \circled4 As to the model otter board with slot position 0.6C, flow speed of the back side was faster 1.3 to 1.7 times than in the front side. \circled5 The size of the separated zone in case of the model otter board with 0.6C was smaller than that of any other models.

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A Study of Japanese Performers in 1915' KEIJO ENGEIKAN : Theater, Performance, and Nakalai ToSui (1915년 경성 연예관의 일본공연단 연구 - 극장, 퍼포먼스, 나카라이 도수이(半井桃水))

  • Hong, Seun-Young
    • Cross-Cultural Studies
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    • v.40
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    • pp.239-264
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    • 2015
  • In the early 20th century, Japan built theaters to take possession of colonial culture while establishing the Imperial University, libraries, and museums to accumulate and spread the knowledge of "imperial" Japan. Many different genres made a hit in theaters including theatrical troupes, theatrical companies, and motion pictures. One of the various features of expositions was "spectacles" or unusual events. They were integrated with a national festival in celebration of "5th anniversary of political commencement." In 1915, the longest hit series in Keijo (Seoul) was the dance performance by professional Japanese beauties and entertainers, whose songs delivered the legitimacy of governance. This study focused on the performance in the space of "cultural" spread called theaters, thus examining the "imperial" cultural waves they were in charge of. The study also focused on the tensional relations of Hierarchie along those cultural borders. The performance at the theater Engeikan(演芸館) in 1915 was especially an epoch-making event in the Japanese theatrical community of Keijo (Seoul): first, it marked the emergence of large-scale performance hall called Engeikan(演芸館); secondly, the performance kept its ongoing, stable streak for about 50 days; and it led to the appearance of leading troupes including Geijutuza(芸術座), which put on a show in Keijo (Seoul) in November, 1915. The study examined the issues of theater Engeikan(演芸館) performance in 1915 involving the art company, performance genre, and audience composition, showed that there was the coexistence of entertainment and governance through cultural ruling while securing amusement and entertainment, and found it was accompanied by the organized operation of "Keijo Sponsorship Council(京城協贊會)" which brought together the cultural capabilities of Japanese people living in Joseon. The performance at theater Engeikan(演芸館) in Keijo (Seoul) in 1915 fully reflected the issues involving the tensional relations between different artistic genres, the competing relations between the subjects of performance, and the cultural power.