Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Attenuation of Atherosclerosis by 3,4-Dihydroxy-Hydrocinnamic Acid in Rabbits by Partial Inhibition of ACAT

토끼에서 ACAT 억제에 의한 3,4-다이하이드록시 하이드로시나믹산의 동맥경화 완화 효과

  • Lee, Mi-Ran (Department of Biomedical Laboratory Science, Jungwon University) ;
  • Choi, Jae-Hoon (Department of Life Science, College of Natural Sciences, Research Institute of Natural Sciences, Hanyang University) ;
  • Yang, Young (Research Center for Women's Disease, Department of Life Science, Sookmyung Women's University) ;
  • Oh, Ki Sook (Research Center for Women's Disease, Department of Life Science, Sookmyung Women's University) ;
  • Jeong, Tae-Sook (Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Chul-Ho (Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Oh, Goo Taeg (Department of Life Sciences, Ewha Womans University)
  • 이미란 (중원대학교 의료보건대학 임상병리학과) ;
  • 최재훈 (한양대학교 자연과학연구소 자연과학대학 생명과학과) ;
  • 양영 (숙명여자대학교 생명과학과 여성질환연구센터) ;
  • 오기숙 (숙명여자대학교 생명과학과 여성질환연구센터) ;
  • 정태숙 (한국생명공학연구원 산업바이오소재연구센터) ;
  • 이철호 (한국생명공학연구원 산업바이오소재연구센터) ;
  • 오구택 (이화여자대학교 생명과학과)
  • Received : 2016.10.25
  • Accepted : 2016.11.18
  • Published : 2016.12.31
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Abstract

Polyphenols have been reported to have beneficial effects on cardiovascular disease. A polyphenolic compound, 3,4-dihydroxy-hydrocinnamic acid (3,4-DHHCA), has been shown to have antioxidative and antitumorigenic activities. However, the effect of 3,4-DHHCA on atherosclerosis is still unknown. Herein, we investigated the effects of 3,4-DHHCA on atherosclerosis in New Zealand White rabbits. Broad and fused fatty streak lesions were found in rabbits fed with high-cholesterol diet for 8 weeks. Administration of 3,4-DHHCA reduced atherosclerotic lesion formation and lesional accumulation of macrophage in rabbits fed with cholesterol diet without systemic or local toxicity. Hepatic acyl-coenzyme A: cholesterol acyltransferase (ACAT) activity was decreased after treatment with 3,4-DHHCA by 22% in cholesterol diet-fed rabbits compared with the control group. These results indicate that 3,4-DHHCA had antiatherogenic effects in rabbits, possibly by partial inhibition of ACAT.

폴리페놀 성분은 심혈관질환에서 좋은 효과를 나타낸다고 보고되고 있다. 폴리페놀성 화합물인 3,4-다이하이드록시 하이드로시나믹산은 항산화 활성과 항암 활성을 나타낸다고 보고되었다. 이 연구의 목적은 3,4-다이하이드록시 하이드로시나믹산이 항동맥 경화 효과를 나타내는지를 뉴질랜드 흰 토끼에서 평가하는 것이다. 8주동안 고콜레스테롤 식이를 급여한 대조그룹 토끼의 광범위한 동맥 부위에서 동맥경화 초기병변이 형성되었다. 반면에 고콜레스테롤 식이를 급여하면서 3,4-다이하이드록시 하이드로시나믹산을 투여한 토끼에서는 대조 그룹의 토끼에 비해 동맥경화 병변 형성이 감소하였고, 병변 내로 침윤한 대식세포의 양도 감소하였다. 이러한 3,4-다이하이드록시 하이드로시나믹산의 효과에서 전신적으로나 국부적으로 독성이 관찰되지 않았다. 간의 아실-코엔자임 A: 콜레스테롤 아실트렌스페라제 활성이 고콜레스테롤 식이를 급여하면서 3,4-다이하이드록시 하이드로시나믹산을 투여한 토끼에서 대조 그룹의 토끼에 비해 22% 감소하였다. 이러한 연구 결과는 3,4-다이하이드록시 하이드로시나믹산이 토끼에서 아실-코엔자임 A: 콜레스테롤 아실트렌스페라제를 억제함으로써 항동맥경화 효과를 나타낸다는 것을 증명해 준다.

Keywords

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