• Title/Summary/Keyword: stomatal aperture

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Eco-physiological Characteristics of Rice Leaves and Wind Damage by Typhoon (태풍에 의한 벼 엽신손상과 엽신 특성과의 관계)

  • 양의석;강양순;정연태;정근식
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.2
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    • pp.201-204
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    • 1988
  • To find out the relationship between the eco-physiological characteristics and wind damage of rice leaves by typhoon 'Thelma' occurred at young panicle formation stage of rice plant, the study was carried out. The rate of dead leaves by wind damage in Indica X Japonica varieties were higher than that in Jap. varieties. Ind. X Jap. varieties which had erect and broader leaves had the higher number of silicified cell, moisture loss and stomatal aperture in leaves. And the wind damage were positvely correlated with the characteristics such as the number of silicified cell (r=0.7546$\^$*/), rate of moisture loss (r=0.8343$\^$**/) and stomatal aperture (r=0.8460$\^$**/) of rice leaf blade etc.

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Light and Electron Microscopy Studies Elucidating Mechanisms of Tomato Leaf Infection by Pseudocercospora fuligena

  • Zelalem Mersha;Girma Birru;Bernhard Hau
    • The Plant Pathology Journal
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    • v.39 no.2
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    • pp.181-190
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    • 2023
  • The fungal pathogen Pseudocercospora fuligena, known to affect tomatoes in the tropics and subtropics, has been reported from temperate climates including the United States and Turkey in recent years. In this study, an isolate from fresh tomatoes and the disease it causes were characterized and infection mechanisms investigated. Macroscopically, both sides of tomato leaves show indistinct effuse patches but prolific production of fuliginous lesions is conspicuous on the abaxial side first but also on the adaxial side later on as infection progressed. Microscopically, fascicles of conidiophores (11-128 ㎛ × 3.5-9 ㎛) arising from stromata and conidia with up to 12 septations were observed. Molecular characterization of the isolate revealed high homology (99.8%) to other P. fuligena isolated from tomatoes in Turkey. Out of the 10 media tested, P. fuligena grew significantly well and sporulated better on unsealed tomato oatmeal agar and carrot leaf decoction agar, both supplemented with CaCO3. Direct transfer of conidia from profusely sporulating lesions was the easiest and quickest method of isolation for in-vitro studies. Light and scanning electron microscopy on cleared and intact tomato leaves further confirmed stomatal penetration and egress as well as prevalence of primary and secondary infection hyphae. In situ, blocked stomatal aperture areas of 154, 401, and 2,043 ㎛2 were recorded at 7, 12, and 17 days after inoculation, respectively. With the recent expanded horizon of the pathosystem and its consequential impact, such studies will be useful for a proper diagnosis, identification and management of the disease on tomato worldwide.

Effects of Elevated $CO_2$ on Maize Growth

  • Kim, Young-Guk;Cho, Young-Son;Seo, Jong-Ho;Kim, Sok-Dong;Shin, Jin-Chul;Park, Ho-Ki
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.1
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    • pp.93-101
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    • 2008
  • The effects of $CO_2$ enrichment on growth of maize (Zea mays L.) were examined. Parameters analyzed include growth characteristics, yields, photosynthetic rates, evaporation rates and photosynthesis-related characteristics under elevated $CO_2$. The plants were grown in growth chambers with a 12-h photoperiod and a day/night temperature of $28/21^{\circ}C$ at the seedling stage and $30/23^{\circ}C$ from the silking stage. The plants were exposed to two elevated $CO_2$ of 500, 700ppm and ambient levels (350 ppm). Chalok 1 and GCB 70 germinated three days after seeding, and germination rates were faster in the elevated $CO_2$ than the control. Germination rates displayed significant differences among the $CO_2$ treatments. At the seedling stage, leaf area, top dry weight, and photosynthetic rates, and plant height indicated positive relationship with elevated $CO_2$ concentrations. At the $5{\sim}6$ leaf stage, $CO_2$ concentration also indicated positive relationship with plant height, leaf area, top dry weight, and photosynthetic rates. At the silking stage, increased plant height of Chalok 1 was noted in the $CO_2$ treatments compared to the control. No significant differences were noted for GCB 70, in which leaf area decreased but photosynthetic rates increased progressively with $CO_2$ concentration. Stomatal aperture was a little bigger in the elevated $CO_2$ than the control. $CO_2$ concentration was negatively related to stomatal conductance and transpiration rates, resulting in high water use efficiency.

Evaluation of Stomatal Characteristics of Adaxial and Abaxial Side of Flag Leaves of Korean Wheat Cultivars

  • Seong-Wook Kang;Ji-Yoon Han;Chang Hyun Choi;Chon-Sik Kang;Swapan Kumar Roy;Seong-Woo Cho
    • Korean Journal of Plant Resources
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    • v.36 no.3
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    • pp.225-236
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    • 2023
  • Stomatal traits such as stomata density (SD), aperture length (APL) and width (APW), guard cell length (GCL) and width (GCW), and distance between stomata (DIS) were investigated to identify correlation with agronomic traits for 35 Korean wheat cultivars. Flag leaf width (FLW) of Korean wheat cultivars was the widest in Ol-mil, and the narrowest in Keumkang. SD tended to be higher on the adaxial side than on the abaxial side in Korean wheat cultivars. SD of adaxial and abaxial sides was classified into a cultivar with a significantly different or not. In APL, 18 wheat cultivars showed significant differences according to leaf side, and APL of adaxial was longer than APL of abaxial in 13 wheat cultivars. In APW, 15 wheat cultivars showed a significant difference, and APW of abaxial was wider than APW of adaxial among them. In GCL, 14 wheat cultivars showed a significant difference, and the GCL of abaxial was longer than the GCL of adaxial in 10 wheat cultivars. In GCW, 10 wheat cultivars showed a significant difference, GCW of adaxial was wider than GCW of abaxial and in 6 wheat cultivars. FLW of adaxial and abaxial showed a negative correlation with GCL and a positive correlation with grain number per panicle. FLW of only abaxial showed a positive correlation with DIS. The SD of the adaxial showed a negative correlation with GCL, while the SD of the abaxial showed a negative correlation with APL. APL of both sides of the leaf showed a positive correlation with GCL, and APW of only abaxial showed a negative correlation with GLC. DIS of adaxial showed a negative correlation with tiller number (TN), while DIS of abaxial showed a positive correlation with GNP.

Effect of simulated Acid rain on Foliar Structural of Changes of Ginkgo biloba and Pinus thunbergii (은행나무와 곰솔에 처리된 인공산성비에 의한 잎의 형태변화)

  • 소웅영
    • Journal of Plant Biology
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    • v.38 no.1
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    • pp.79-86
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    • 1995
  • Visible injury symptoms such as necrosis, chlorosis and premature senscence in the leaves of Ginkgo bloba and Pinus thunbergii treated with acid rain of pH 3.2 or below were observed. The epicuticular wax erosions were observed by SEM after exposure to acid rain of pH 2.4 and 3.2 in G. biloba and pH 4.0 below in P. thunbergii. The adaxial epidennal cells and sponge parenchyma cells were compressed, and those were distorted in the leaves of G. biloba treated with simulated acid rain of pH 3.2 or below. However, vascular tissue was intact. With increase of acidity, mesophyll cells were smaller than those of control while intercellular space in mesophyU was increased. In P. thunbergii, sponge parenchyma cells and vascular tissue except epidennis were distorted after exposure to acid rain of pH 2.4. The size change of stomata in foliar injury was not observed, but the stomatal index and size of stomatal aperture in leaves treated with acid rain increased. The stomata of injured leaf were opened in both species examined.amined.

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The Effects of Water Stress on C$_3$ Plant and CAM Plant (C$_3$ 식물과 CAM 식물에서 수분 스트레스의 효과)

  • An, Du-Hwan;Kim, Yong-Taek;Kim, Dae-Jae;Lee, Joon-Sang
    • Korean Journal of Environmental Biology
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    • v.26 no.4
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    • pp.271-278
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    • 2008
  • The differences of several kinds of physiological responses between Commelina communis (C$_3$ plant) and Sedum sarmentosum (CAM plant: Crassulacean Acid metabolism) when both plants were exposed to water stress for 3 weeks were investigated. In case of Commelina it was shown a clear loss of water to 12% in three weeks, but no changes were observed in Sedum. Total chlorophyll content was also reduced to 57% in Commelina but not clear changes of chlorophyll content in Sedum. were observed for three weeks. In chlorophyll fluorescence experiments Fv/Fm ratios were reduced to 19% in Commelina, but no changes were observed in Sedum. There were very sensitive responses according to the different KCl concentrations and the stomatal aperture of epidermal strips was 12.8 ${\mu}m$ at 200 mM KCl in Commelina, but less than 3 ${\mu}m$ was observed at the same KCl concentration in Sedum. In addition, there were no chloroplasts in guard cells of Sedum, but most plants had chloroplasts including Commelina. From the above results, the ability of water stress resistance in Sedum. could be come from slow physiological metabolism including growth and less loss of water through unique stomatal characteristics.

Effect of Vapor Pressure Deficit on the Evapotranspiration Rate and Graft-taking of Grafted Seedling Population under Artificial Lighting (인공광하에서 접목묘 개체군의 증발산속도와 활착에 미치는 포차의 영향)

  • Yong Hyeon Kim;Chul Soo Kim;Ji Won Lee;Sang Gyu Lee
    • Journal of Bio-Environment Control
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    • v.10 no.4
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    • pp.232-236
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    • 2001
  • Four air temperature levels of 23, 25, 27 and 29$^{\circ}C$, three humidity levels of 85, 90 and 95% R.H. at photosynthetic photon flux (PPF) of 50 $\mu$mol.m$^{-2}$ .s$^{-1}$ were provided to investigate the effect of vapor pressure deficit on the evapotranspiration rate (EVTR) and graft-taking of watermelon grafted seed-increase. Thus EVTR of grafted seedlings increased with increasing air temperature at high humidity of 95%R.H. At relatively low humidity of 85% R.H., grafted seedlings showed a high EVTR and some wilting of scions was observed at this condition. This result would be ascribed to the low supply of water to vascular bundles according to the insufficient joining of scions and rootstocks. Differences in EVTR between 90% R.H. and 95% R.H. were not observed. Grafted seedlings showed high graft-taking at high relative humidity. Relative humidity had highly influenced to the graft-taking as compared to the air temperature. Graft-taking increased with decreasing vapor pressure deficit. Graft-taking greater than 90% was found at vapor pressure deficit less than 0.4kPa which could be obtained at humidity higher than 90% R.H. Therefore it is required to control the humidity higher than 90% R.H. for suppressing EVTR of grafted seedlings and preventing some wilting of scoins and thus enhancing the graft-taking of grafted seedlings.

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Taxonomic study on viola albida var. albida and its related taxa (태백제비꽃과 근연분류군의 분류학적 연구)

  • Jang, Su-Kil;Lee, Woo-Tchul;Yoo, Ki-Oug
    • Korean Journal of Plant Taxonomy
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    • v.36 no.3
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    • pp.163-187
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    • 2006
  • Taxonomic studies were conducted to evaluate the interspecific relationships in Viola albida var. albida and its related taxa using seven populations for morphology, palynology and anatomy. Molecular phylogenetic studies were also examined in 28 populations including 19 Korean, four Chinese, two Japanese, one American population and two outgroups using nrITS, and 27 populations except V pinnata for trnL-F region of chloroplast DNA. Morphological differences was observed among seven populations of three species in leaf shape, but characters such as serrate number of leaf margins, petal size, pistil shape were showed overlap between populations. Pollen shape of seven populations was monad and grain shape on the polar axis was semi-angular. Morphology of aperture was tri-colporate, and the surface sculpturing was scabrate in rugulate. The grain shape of equatorial view of five populations was prolate whereas V albida var. taknhashii type 1 and V albida var. chaerophylloides type 3 were subprolate. The anatomical characters of rnidvein of leaf, petiole, peduncle, root were also described for the species. The stomatal apparatus of the leaves was observed only in abaxial surface, and the number of stoma per unit ($mm^2$) were abundant in incised than lobate or cleft margin of the leaves. The nrITS analysis shows that V. pinnata and V. dissecta was monophyletic and occupied a basal position in the V. albida var. albida and its related taxa. The other clade including infraspecific populations of V. albida, and V. eizanensis was paraphyletic. The trnL-F noncoding region analysis was similar to the ITS tree. According to the above observations in morphology, palynology, anatomy, and molecular phylogenetic analysis, the significant differences were not found except for leaf shape in Viola albida var. albida and its related taxa, therefore V. albida var. takanhashii and V. albida var. chaerophylloides were considered to be an infraspecific taxa of V. albida var. albida rather than an independent species, subvariety or variety of V. pinnata and V. dissecta.

Effect of Low Light Intensity after Fruit Set on the Growth and Quality of Cucumber(Cucumis sativus cv. Hyakunari-3). (착과기 이후의 약공 조건이 백침게 오이의 생육반응 및 품질에 미치는 영향)

  • Seong, Ki-Cheol;Moon, Gi-Hye;Lee, Sang-Gyu;Kang, Yong-Gu;Kim, Kwang-Yong;Seo, Hyo-Duck
    • Journal of Bio-Environment Control
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    • v.13 no.1
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    • pp.44-50
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    • 2004
  • This experiment was conducted to investigate the effects of different levels of light intensity (100, 200, 400 ${\mu}mol\;{\codt}\;m^{-2}\;{\cdot}\;s^{-1}$, and natural light) on the growth and the fruit quality of cucumber(Cucumis sativus cv. Hyakunari-3). The results of this experiment indicated that plant height and length of lateral shoots were decreased under low light condition, but it was not significantly different among treatments. Leaf area and root weight were lowest under low light intensity(100 ${\mu}mol\;{\codt}\;m^{-2}\;{\cdot}\;s^{-1}$), but no significant differences were noted between 200 and 400 ${\mu}mol\;{\codt}\;m^{-2}\;{\cdot}\;s^{-1}$. Photosynthesis rate was decreased with reduced light intensity and total chlorophyll contents, root activity and xylem sap were also decreased under low light intensity, but there was no significant difference between 200 and 400 ${\mu}mol\;{\codt}\;m^{-2}\;{\cdot}\;s^{-1}$. From the SEM observation the erosion of the guard cells and closed stomata in low light treatment were shown and the size of stoma were small also the stomatal aperture were decreased with reducing the light intensity. Chlorosis in leaves and aborted-liked fruits were appeared under low light condition and Ca and Mg uptake in leaves were decreased by shading in proportion to the decrease of light intensity. Fruit yields were decreased by 65% under 400 ${\mu}mol\;{\codt}\;m^{-2}\;{\cdot}\;s^{-1}$, and by 80${\sim}$90% under 200 and 100 ${\mu}mol\;{\codt}\;m^{-2}\;{\cdot}\;s^{-1}$, compared to those under the natural light. This low intensity of light caused the sharp decrease in the early harvested yields within two weeks and the fruit yields of lateral shoots were greatly decreased.