• Journal of Korean Society of Forest Science
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• v.97 no.5
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• pp.552-554
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• 2008

Acer palmatum was selected and its responses to elevated ozone were subsequently measured during growing periods. Ozone concentration of this study was compared to the calculated AOT40 value. A. palmatum had significantly many but small stomata size to the ozone stress. The length of stomata of A. palmatum was reduced from 5.6 to $5.0{\mu}m$ to the ozone exposure. However, the number of stomata (density) was increased from 102 to 131 in the $500{\times}500{\mu}m$ leaf area.

• Journal of Korean Society of Forest Science
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• v.76 no.1
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• pp.27-32
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• 1987

Stomatal morphological characteristics (shape, density and length) were investigated for 8 ornamental woody species growing in the campus of Sangji College on Usandong, Wonju, Kangwondo. Stomata of Forsythia koreana, Syringa dialata, Zedkova serrata and Chaenomeles sinensis were classified as irregular-celled type (anomocytic), those of Vibrunum sargentii, Liriodendron tulipifera and Magnolia kobus as parallel-celled type (paracytic). For Forsythia koreana, stomatal length was not greatly changed as the leaf blade, growing larger. Number of stomata per unit leaf area was decreased, but number of stomata per single leaf was increased as the leaf blade, growing larger. For Taxus cuspidata, stomatal length was increased, but number of stomata per unit needle area was decreased as the needle, growing larger.

• Applied Microscopy
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• v.42 no.3
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• pp.124-128
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• 2012

Leaf surface structures were investigated in the Chinese juniper Juniperus chinensis by scanning electron microscopy. Adult scale leaves were collected from the tree, air-dried at room temperature, and sputter-coated with gold without further specimen preparation. Approximately fi ve stomata were locally distributed and arranged in clusters on the leaf surface. Stomata were ovoid and ca. 40 ${\mu}m$ long. The epicuticular wax structures of J. chinensis leaves were tubules and platelets. Numerous tubules were evident on the leaf regions where stomata were found. The tubules were cylindrical, straight, and ca. 1 ${\mu}m$ in length. They almost clothed the stomatal guard cells, and occluded the slit-shaped stomatal apertures. Moreover, the wax ridges were flat crystalloids that were connected to the surface by their narrow side. They did not have distinct edges, and their width/height ratio varied. In particular, the wax ridges could be discerned on the leaf regions where stomata were not present nearby. Since the wax ridges did not have distinct edges on their margin, they were identified as platelets. Instances were noted where platelets were oriented either parallel to each other or perpendicular to the cuticle surface. These results can be used in biomimetics to design the hierarchical structures for mimicking the plant innate properties such as hydrophobicity and self-cleaning effects of the leaf surface.

• Applied Microscopy
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• v.42 no.3
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• pp.147-150
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• 2012

A recently introduced helium ion microscopy (HIM) was employed to observe uncoated pine leaf specimens. Adult leaves were collected from the seedlings of Pinus densiflora and P. rigida, air-dried at room temperature, and observed by HIM without metal coating. Ovoid or round stomata and distinct Florin rings could be discerned. The epicuticular waxes were present in the epistomatal chambers and Florin rings of stomata on the leaf surface. The epicuticular waxes were mostly straight, cylindrical, and ca. 1 ${\mu}m$ in length. The epistomatal chambers of P. rigida were filled with the epicuticular waxes, whereas those of P. densiflora were not filled with the epicuticular waxes. Based on their micromorphology, the epicuticular wax structures of the pine species were identified as tubules. These results suggest that the HIM could be used for the investigation of the plant stomata and epicuticular waxes of uncoated plant leaves. Due to the smaller ion probe and interaction volume, the HIM has advantages over conventional field emission scanning electron microscopy in terms of image resolution and charge neutralization.

• Journal of Korean Society of Forest Science
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• v.57 no.1
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• pp.39-44
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• 1982

These studies were carried out to know the variation of needle characteristics of Abies Koreana distributed in Korea. The results are summarized as follows. The differences of needle length and number of stomata row among the population were shown one percent level, but not in each individual tree. Correlations coefficients between the D.B.H. and needle length or needle width of mother trees were not correlated all populations respectively. It was found that correlation coefficient between needle length and needle width was positive in two populations. of Mt. Doukyou and Mt. Hanla, but it was not in Mt Jiri. Correlation coefficient between needle length and the number of stomata row positive correlation in Mt. Doukyou. Correlation coefficient between needle width and the number of stomata row was shown positive in all populations, but it was not that correlation coefficient between needle width and density of serrations, and between number of stomata row and density of serrations. The coefficients of variations were shown higher density of serration than needle length, needle width and stomata row.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.68 no.3
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• pp.216-223
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• 2023

This study aimed to identify and compare the characteristics of Elymus humidus on common wheat (Triticum aestivum L. cv. Chinse Spring, CS). The seed length, width, height, and weight of E. humidus were smaller than those of the CS. In particular, the germination rate of E. humidus was substantially lower than that of CS. In the anatomical dissection of the leaf, E. humidus showed a considerably different xylem diameter of the main vascular bundle in the main vein; however, there was no difference in the phloem of the main vascular bundle compared with the xylem and phloem of the main vascular bundle in the main vein of CS, although E. humidus showed a leaf structure similar to that of CS. In addition, E. humidus had a thinner epidermis than that of CS. Regarding stomatal traits, E. humidus showed a graminoid stomata type similar to that of CS. On the adaxial and abaxial sides, the density, length, and width of the stomata in E. humidus were smaller than those in CS, whereas the distance between stomata in E. humidus was greater than that in CS. The chromosomes of E. humidus were classified as long and short based on their respective lengths. Long chromosomes were classified based on the ratio of the long arm to the short arm e.g., 1:1 or 2:1. Short chromosomes showed the same trend and some short chromosomes were microsatellites. To evaluate genetic diversity, 38 barley EST markers with polymorphisms between E. humidus and CS were selected from 236 barley EST markers.

• Korean Journal of Plant Resources
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• v.23 no.1
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• pp.31-37
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• 2010

It was investigated that morphological characteristics of leaf and stomata, chlorophyll contents and wood anatomy characteristics in Schisandra chinensis, Schisandra nigra and Kadsura japonica. Leaf morphological characteristics were significant at 5% levels within the species and within the individuals as a male, a female and monoecious. K. japonica was the longest in the leaf length, followed by S. chinensis and S. nigra. For the leaf length among S. nigra individuals, the female was longer than male and monoecious. As for the Petiole length, S. nigra was twice longer than S. chinensis and K. japonica. Stomata length was ranged from $92.5\;{\mu}m$ to $105.9\;{\mu}m$ for S. nigra, $79.9\;{\mu}m$ for S. chinensis, $76.2\;{\mu}m$ for K. japonica. S. nigra was longer than the others in length and width, while its stomata density was lower than the others. The size of vessel elements and xylem fiber showed a similar tendency to stomata, significant at 5% levels within S. nigra individuals (male, female and monoecious). Chlorophyll contents were also variable in inter and intra species and in general K. japonica showed higher levels of chlorophyll contents than Schisandra spp. plants.

• Korean Journal of Plant Resources
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• v.22 no.6
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• pp.506-512
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• 2009

To study the effect of genotype of donor plants on anther culture, anthers of nine hybrid cultivars (Derby, Special, Bossanova, Minipaprika, Fiesta, Boogie, Phenlene, Kufrah, and Clarity) of sweet pepper (Capsicum annuum L.) were cultured in a petridish containing C medium (Sibi, Dumas De Vaulx medium) supplemented with 0.1 mg/L 2,4-D and 0.1 mg/L kinetin, 3% sucrose and 0.32% Phytagel. The cultures were incubated in the dark at $35^{\circ}C$ for seven days, and then cultured at $25^{\circ}C$ with a photoperiod of 16 hr. daylength for 40 days. Frequency of callus formation and plant regeneration was varied among cultivars. Callus formation was ranged from 6% in Phenlene to 69.8% in Kufrah. The highest percentage of regenerated plantlets was obtained in cv. Phenlene (2.67%) followed by Bossanova (2.41%). Result of ploidy analysis; chromosome number observation and flowcytometry analysis, showed that haploid plants could be developed from all of these hybrid cultivars except cv. Fiesta, where highest percentage of haploid plants were obtained in Minipaprika (40%) followed by cv. Bossanova (36.1%). Haploid plants derived from these hybrid cultivars contained single set of chromosome (12 in numbers), higher stomata density (numbers), and smaller sized stomata as compare to diploid plants. The mean length of stomata was 26.9 ${\mu}m$ in haploid plants and 35.7 ${\mu}m$ in diploids.

• Journal of Korean Society of Forest Science
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• v.82 no.3
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• pp.260-270
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• 1993

This study was carried out to clarifyed a taxonomical relationships of Rutaceae on the subfamily, genus and species level in Korea using venation pattern and stomata types. The results are summarized as follows : The leaf venation of the Rutaceae has revealed three major venation types. Semicraspedodromous, Brochidodromous and Cladodromous. The leaf ufmations type of the genus Evodia and Phellodendron were similar to Cladodromous type. They showed gradual evolution from brochidodromous type of Aurantioideae to cladodromous type of Toddaloideae. The stomata ypes were reconized Anomocytic and Actinocytic type, and epidermal cells were Ansio. Polygonal and Medium type. In the correlation analysis, there were highly significant correlation between frequency and length (r=-0.822), frequency and width (r=-0.780), and length and width(r=0.909) at 1% level.

• Journal of Ginseng Research
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• v.26 no.2
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• pp.85-88
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• 2002

This study was carried out to obtain information of the photosynthetic rate at various temperature and light intensity, stomata, chlorophyll, specific leaf weight, characteristics of aerial part and root in ginseng new cultivars developed by pure line selection. The light saturation point of leaves in new cultivars and Jakyungjong were 15,000 lux, and the optimum air temperature on the photosynthesis of new cultivars and Jakyungjong were 20$\^{C}$. The photosynthetic rates were increased in order of Jakyungjong, Gopoong, Chunpoong and Yunpoong. The dark respiration rate of leaves in ginseng cultivars were increased according to the increasing of temperature, and the dark respiration rate of leaves of Yunpoong was the highest among cultivars. The specific leaf weight (SLW) were increased in order of Jakyungjong, Yunpoong, Gopoong, Chunpoong, but total chlorophyll contents were not different among cultivars. Stomata frequency of Yunpoong was the highest being 69.2ea among cultivars, while the length of stomata was reverse. Yunpoong was superior in aerial part among ginseng cultivars : the number of stem was 1.8ea, the number of palmately leaves was 7.7ea, the number of leaflets was 41.0ea, leaf area was 12.3 dm$^2$ The root weight were increased in order of Jakyungjong, Gopoong, Chunpoong and Yunpoong. Chunpoong and Gopoong hove good root shape the length of tap root in Chunpoong and Gopoong were the longest being 6.5 cm and 6.8 cm respectively, but that in Yunpoong was the shortest being 4.4 cm.