Browse > Article

Effect of Genotype of Donor Plants on the Success of Anther Culture in Sweet Pepper (Capsicum annuum L.)  

Shrestha, Surendra Lal (Nepal Agriculture Research Council)
Kang, Won-Hee (Department of Horticulture, Kangwon National University)
Publication Information
Korean Journal of Plant Resources / v.22, no.6, 2009 , pp. 506-512 More about this Journal
Abstract
To study the effect of genotype of donor plants on anther culture, anthers of nine hybrid cultivars (Derby, Special, Bossanova, Minipaprika, Fiesta, Boogie, Phenlene, Kufrah, and Clarity) of sweet pepper (Capsicum annuum L.) were cultured in a petridish containing C medium (Sibi, Dumas De Vaulx medium) supplemented with 0.1 mg/L 2,4-D and 0.1 mg/L kinetin, 3% sucrose and 0.32% Phytagel. The cultures were incubated in the dark at $35^{\circ}C$ for seven days, and then cultured at $25^{\circ}C$ with a photoperiod of 16 hr. daylength for 40 days. Frequency of callus formation and plant regeneration was varied among cultivars. Callus formation was ranged from 6% in Phenlene to 69.8% in Kufrah. The highest percentage of regenerated plantlets was obtained in cv. Phenlene (2.67%) followed by Bossanova (2.41%). Result of ploidy analysis; chromosome number observation and flowcytometry analysis, showed that haploid plants could be developed from all of these hybrid cultivars except cv. Fiesta, where highest percentage of haploid plants were obtained in Minipaprika (40%) followed by cv. Bossanova (36.1%). Haploid plants derived from these hybrid cultivars contained single set of chromosome (12 in numbers), higher stomata density (numbers), and smaller sized stomata as compare to diploid plants. The mean length of stomata was 26.9 ${\mu}m$ in haploid plants and 35.7 ${\mu}m$ in diploids.
Keywords
Anther cultured plantlets; callus; Capsicum annuum L.; embryoid; pure lines; regeneration;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Pochard, E., K.G. Selassie, and G. Marchoux. 1983. Oligogenic resistance to potato virus Y pathotype 1-2 in the line 'Perennial'. Capsicum NWsl 2: 137-138
2 Brown, D.C.W. and T.A. Thorpe. 1994. Crop improvement through tissue culture. World Journal of Microbiology and Biotechnology. 11: 409-415   DOI   ScienceOn
3 Dumas, D.V.R, D. Chmbonnet, and E. Pochard. 1981. Culture in vitro D'atheres de piment (Capsicum annuum L.): amelioration des tuax d'obtention de plantes chez differents genotype par des traitments $a+35{^{\circ}C}$. Agronomice. 1: 859- 864   DOI
4 Keller, W.A. and K.G. Amstrong. 1983. Production of haploids via anther culture in Brassica oleracea var. Italica. Euphytica 32: 151-159   DOI
5 Matsubara S., M. Yamamoto, M.H. Jo, and K. Murakami. 1998. Embryoid and callus formation from microspores by anther culture from July to November in pepper (Capsicum annuum L.). Sci. Rep. Fac. Agri. Okayama Univ. 87: 117- 122
6 Yoon, Y.J., S.K. Lee, S.K. Chang and S. Kung. 1991. Plant induction by anther culture of Hot pepper (Capsicum annuum L.) J. Kor. Soc. Hort. Sci. 32(1): 8-16
7 Sakata Y., T. Fujiki, and S. Monma. 1991. Varietal difference in embrogenic ability in anther culture of Capsicum. J. Japan Soc. Hort. Sci. 60(suppl.1): 204-205
8 Bajaj, Y.P.S. 1977. In vitro induction of haploids in wheat (Triticum aestivum L.). Crop improve. 4: 54-64
9 Eun, J.S., K.S. Lee, and Y.J. Yoon. 1994. Effect of medium composition, heat treatment and genotypes on embryogenesis and plant regeneration, and genetic variation of derived plantlets in anther culture of Capsicum annuum. Kor. J. Breed. 26: 353-362
10 Matsubara S., K.L. Hu, and K. Murakami. 1992. Embryoid and callus formation from pollen grains of eggplant and pepper by anther culture. J. Japan Soc. Hort. Sci.61: 69-77   DOI
11 Abak, K., E. Pochard, and D.V.R. Dumas. 1982. Transmission of resistance to Phytophthora capsici on roots and stems of pepper plants; study of doubled haploid lines issued from the cross 'PM 217 x Yolo wonder' through anther culture. Capsicum NWsl 1: 62-63
12 Nowak, A.N. and P. Nowaczyk. 2005. Hybrids of Capsicum frutescens X Capsicum annuum L. as a material for androgenic haploid production. In ACTA BIOLOGICA CRALOVIESIA Series, Botanica 47, suppl. 1, 2005
13 Qin, X., and G.L. Ratino. 1995. Anther culture of several sweet and hot pepper genotypes. Acta Horticulture. PP. 402
14 Kristiasen K. and S.B. Andersen. 1993. Effects of donor plant temperature, photoperiod, and age on anther culture response of Capsicum annuum L. Euphytica 67: 105-109   DOI
15 Daubeze, A.M., Palloix, A., and Pochard, E. 1990. Resistance of androgenetic autodiploid lines of pepper to Phytophthora capsici tobacco mosaic virus under high temperature. Capsicum NWsl 8-9: 47-48
16 Harn, C., M.Z. Kim, K.T. Choi, and Y.I. Lee. 1975. Production of haploid from the cultured anther of Capsicum annuum. SABRAOJ 7: 71-77
17 Tyagi, A.P. and S. L. Kancherla. 1991. Chromosome analysis in a natural and micropropagated Australian ornamental climbers. Department of Bilogy, University of South Pacific, Fiji. PP. 66
18 Hendy, H., E. Pochard, and A. Dalmasso. 1985. Transmission de la resistance aux nematodes Meloidogyne chitwood (Tylenchida) Portee Par 2 lignees de Capsicum annuum L. ; Etude de descendances homozygotes issue d' androgenese. Agronomie 5: 93-100   DOI
19 Chahal, G.S. and S.S. Gosal. 2002. Principles and procedures of plant breeding. Alpha Science International Ltd. Pangbourne. PP.87, 413, 547
20 Kim, Y.W., O.Y. Kweon, and M.W. Yoon. 1999. Influence of genotype and ecotype on anther culture efficiency in hot pepper (Capsicum annuum L.). Kor. J. Plant Tissue culture 26: 49-52   과학기술학회마을
21 Dumas, D.V.R. and E. Pochard. 1986. Parthenogenise et androgenese chez le piment. Role and actuel dans les programmes de selection. Le Selectionneur Francais 36: 3- 16. (In French)
22 Guha, S. and S.C. Maheshwari. 1964. In vitro production of embryos from anthers of Datura. Nature 204: 497   DOI
23 Hu, H. and I.Z. Zeng. 1984. Development of new varieties via anther culture. Handbook of Plant Cell Culture. Vol. 3. P.V. Ammirato, D.A. Evans, W.R. Sharp and Y. Yamada (eds.), New York, Macmillan. PP. 65-90
24 Bajaj Y.P.S. 1990. In vitro production of haploids and their use in cell genetics and plant breeding. In: Biotechnology in agriculture and Forestry. Haploids in crop improvement I. Springer Verlag, Berlin PP. 3-44
25 Caranta, C., Palloix, A., Gebre, S.K., Lefebvre, V. Moury, B., and Daubeze A.M. 1996. A complementation of two genes originating from susceptible Capsicum annuum lines confers a new and complete resistance to pepper veinal mottle virus. Phytopathology 86: 739-743   DOI   ScienceOn
26 Guha, S. and S.C. Maheshwari. 1966. Cell division and differentiation of embryos in the pollen grains of Datura in vitro. Nature 212: 97-98   DOI
27 Wang Y., C. Sun, C. Wang, and N. Chien. 1973. The induction of the pollen plantlets of Triticale and Capsicum annuum from anther culture. Scienta Sincia 16: 147-151
28 Przywara, L., K.K. Pandey, and P.M. Sanders. 1988. Length of stomata as an indicator of ploidy level in Actinidia deliciosa. New Zealand Journal of Botany, 26: 179-182   DOI
29 Evans, D.A., W.R. Sharpe, and J.E. Bravo. 1984. Cell culture methods for crop improvement. Handbook of Plant Cell Culture, vol. 2, eds W.R. Sharpe, D.A. Evans, P.V. Ammirito and Y. Yamada. NewYork, Macmillan. PP.393-441