• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제32권4호
• /
• pp.327-333
• /
• 2006

Future cell-based therapies such as tissue engineering will benefit from a source of autogenous pluripotent stem cells. There are embryonic stem cells (ESC) and autologous adult stem cells, two general types of stem cells potentilally useful for these applications. But practical use of ESC is limited due to potential problems of cell regulation and ethical considerations. To get bone marrow stem cells is relatively burden to patients because of pain, anesthesia requirement. The ideal stem cells are required of such as the following advantages: easy to obtain, minimal patient discomfort and a capability of yielding enough cell numbers. Adipose autologus tissue taken from intraoral fatty pad or abdomen may represent such a source. Our study designed to demonstrate the ability of human adipose tissue-derived stromal cells (hATSC) from human abdominal adipose tissue diffentiating into osteocyte and adipocyte under culture in vitro conditions. As a result of experiment, we identified stromal cell derived adipose tissue has the multilineage potentiality under appropriate culture conditions. And the adipose stromal cells expressed several mesenchymal stem cell related antigen (CD29, CD44) reactions. Secondary, we compared the culture results of a group of hATSC stimulated with TGF-${\beta}$1, bFGF with a hATSC group without growth factors to confirm whether cytokines have a important role of the proliferation in osteogenic differentiation. The role of cytokines such as TGF-${\beta}$1, bFGF increased hATSC's osteogenic differentiation especially when TGF-${\beta}$1 and bFGF were used together. These results suggest that adipose stromal cells with growth factors could be efficiently available for cell-based bone regeneration.

• Advances in Traditional Medicine
• /
• 제7권1호
• /
• pp.46-50
• /
• 2007

Callus cultures of Convolvulus microphyllus Sieb. was induced on Murashige and Skoog's medium supplemented with 2,4-dichloro phenoxy acetic acid, 6-benzyl adenine, indole acetic acid and kinetin (1 ppm each). Methanolic extracts of whole plant, leaf, stem and leaf and stem calli were tested for anticonvulsant activity against standard drug phenytoin using maximal electroshock model on mice. It was observed that the animals treated with methanolic extracts of stem callus, leaf callus and whole plant (200 mg/kg, oral) showed significant protection against tonic convulsions induced by transcorneal electroshock. Anticonvulsant activity of methanolic extract of stem callus was comparable to that of standard drug phenytoin.

• 대한음성언어의학회:학술대회논문집
• /
• 대한음성언어의학회 2003년도 제19회 학술대회
• /
• pp.183-183
• /
• 2003

Background and Objectives : Cartilage reconstruction is one of medical issue in otolaryngology. Tissue engineering is presently being utilized in part of cartilage repair. Sources of cells for tissue engineering are chondrocyte from mature cartilage and bone marrow mesenchymal stem cells that are able to differentiate into chondrocyte. Recent studies have shown that adipose tissue have mesenchymal stem cells which can differentiate into adipogenic, chondrogenic myogenic osteogenic cells and neural cell in vitro. In this study, we have examined chondrogenic potential of the canine adipose tissue-derived mesenchymal stem cell(ATSC). Materials and Methods : We harvested canine adipose tissue from inguinal area. ATSCs were enzymatically released from canine adipose tissue. Under appropriate culture conditions, ATSCs were induced to differentiate into the chondrocyte lineages using micromass culture technique. We used immunostain to type II collagen and toluidine blue stain to confirm chondrogenic differentiation of ATSCs. Results : We could isolate ATSCs from canine adipose tissue. ATSCs expressed CD29 and CD44 which are specific surface markers of mesenchymal stem cell. ATSCs differentiated into micromass that has positive response to immunostain of type II collagen and toluidine blue stain. Conclusion : In vitro, ATSCs differentiated into cells that have characteristic cartilage matrix molecules in the presence of lineage-specific induction factors. Adipose tissue may represent an alternative source to bone marrow-derived MSCs.

• BMB Reports
• /
• 제52권5호
• /
• pp.295-303
• /
• 2019

Breakthroughs in stem cell technology have contributed to disease modeling and drug screening via organoid technology. Organoid are defined as three-dimensional cellular aggregations derived from adult tissues or stem cells. They recapitulate the intricate pattern and functionality of the original tissue. Insulin is secreted mainly by the pancreatic ${\beta}$ cells. Large-scale production of insulin-secreting ${\beta}$ cells is crucial for diabetes therapy. Here, we provide a brief overview of organoids and focus on recent advances in protocols for the generation of pancreatic islet organoids from pancreatic tissue or pluripotent stem cells for insulin secretion. The feasibility and limitations of organoid cultures derived from stem cells for insulin production will be described. As the pancreas and gut share the same embryological origin and produce insulin, we will also discuss the possible application of gut organoids for diabetes therapy. Better understanding of the challenges associated with the current protocols for organoid culture facilitates development of scalable organoid cultures for applications in biomedicine.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권11호
• /
• pp.6549-6556
• /
• 2013

Leukemia stem cells (LSCs) play important roles in leukemia initiation, progression and relapse, and thus represent a critical target for therapeutic intervention. Hence, it is extremely urgent to explore new therapeutic strategies directly targeting LSCs for acute myelogenous leukemia (AML) therapy. We show here that Angelica sinensis polysaccharide (ASP), a major active component in Dong quai (Chinese Angelica sinensis), effectively inhibited human AML $CD34^+CD38^-$ cell proliferation in vitro culture in a dose-dependent manner while sparing normal hematopoietic stem and progenitor cells at physiologically achievable concentrations. Furthermore, ASP exerted cytotoxic effects on AML K562 cells, especially LSC-enriched $CD34^+CD38^-$ cells. Colony formation assays further showed that ASP significantly suppressed the formation of colonies derived from AML $CD34^+CD38^-$ cells but not those from normal $CD34^+CD38^-$ cells. Examination of the underlying mechanisms revealed that ASP induced $CD34^+CD38^-$ cell senescence, which was strongly associated with a series of characteristic events, including up-regulation of p53, p16, p21, and Rb genes and changes of related cell cycle regulation proteins P16, P21, cyclin E and CDK4, telomere end attrition as well as repression of telomerase activity. On the basis of these findings, we propose that ASP represents a potentially important agent for leukemia stem cell-targeted therapy.

• Journal of Plant Biotechnology
• /
• 제30권3호
• /
• pp.257-261
• /
• 2003

This experiment was carried out to investigate the effects of plant growth regulators on the organogenesis from the tissue culture of Arabidopsis thaliana stem, and the origin of the callus development. When the stem segments were cultured on medium with 2mg/L IAA or NAA, adventitious roots and trichomes were differentiated after 11 days of culture. Callus vigorously formed on medium with 2/L2,4 after 7 days of culture, but adventitious roots and trichomes were not differentiated from callus after 10 days of culture. This results suggesting that picloram is very effective auxin for the callus formation and organogenesis. Callus weakly formed on 0.05mg/L kinetin, and formed on combination of auxins(2mg/L) with 0.05mg/L kinetin. But the effect of combination of auxins and kinetin the callus formation was less than 2,4-D or picloram alone. A histological examination of callus formed on picloram showed that phloram showed that phloem parenchyma cells were divided and enlarged after 2 days of culture. Cortex parenchyma cells were divided and meristematic nodules were developed from these cells after 4 days of culture. Finally, callus formed on outside of cortex and epidermis by division of meristematic nodules after 7 days of culture.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제48권2호
• /
• pp.71-78
• /
• 2022

This study was conducted to review the efficacy of different sources of stem cells in bone regeneration of cleft palate patients. The majority of previous studies focused on the transplantation of bone marrow mesenchymal stem cells. However, other sources of stem cells have also gained considerable attention, and dental stem cells have shown especially favorable outcomes. Additionally, approaches that apply the co-culture and co-transplantation of stem cells have shown promising results. The use of different types of stem cells, based on their accessibility and efficacy in bone regeneration, is a promising method in cleft palate bone regeneration. In this regard, dental stem cells may be an ideal choice due to their efficacy and accessibility. In conclusion, stem cells, despite the lengthy procedures required for culture and preparation, are a suitable alternative to conventional bone grafting techniques.

• 한국약용작물학회지
• /
• 제2권2호
• /
• pp.154-161
• /
• 1994

폐모(Fritillaria thunbergii)의 자구인편, 줄기, 및 마디조직 및 정단을 구명하고자 실험한 결과는 다음과 같다. 1. 자구형성수는 자구인편조직에 비해 마디나 줄기조직에서, BA처리구보다 kinetin처리구에서 현저히 증가하였다. 줄기조직은 생장이 3cm이하일 때 채취하여 kinetin이 $1.0{\sim}3.0mg\;/L$첨가된 배지에 배양하였을 , 마디조직은 3cm이상 생장한 조직을 kinet이 5.0mg /L 첨가된 배지에 배양하였을 때 자구형성수가 증가하였다. 기내에서 생장한 마디의 액아에서 자구가 직접 형성되는 현상도 관찰되었는데 최고 15개의 자구가 마디에서 형성되었다. 2. 신초가 l0cm 이상 생장한 후 줄기조직을 채취하여 배양하였을 때는 어린 줄기조직보다 자구 형성능력이 감소하였으며 생장조절제의 첨가가 자구형성에 필수적이었고 적정농도는 kinetin 1.0 mg /L였다. 3. 지하에서 생장중인 패모에서는 줄기조직이나 정단이나 자구조직에 비해 구형성율이 높았으며 자구 형성에 적합한 당의 농도는 $5{\sim}7%$였다. 4. 이상의 결과를 종합해 보면 패모의 기내 배양재료로는 자구 인편조직에 비해 3cm정도 생장한 줄기나 마디조직을 이용하는 것이 대량중식에 효과적일 것으로 생각되었다. 특히 줄기나 마디조직은 구형성수가 최고 20배 이상 달하여 중식율이 상당히 높았다. 생장조절제 요구도는 배양재료에 따라 다소 차이가 있으나 Kinetin $1.0{\sim}5.0mg\;/L$가 적합하였다.

• Anatomy and Cell Biology
• /
• 제56권4호
• /
• pp.508-517
• /
• 2023

In cancer patients, chemo/radio therapy may cause infertility by damaging the spermatogenesis affecting the self-renewal and differentiation of spermatogonial stem cells (SSCs). In vitro differentiation of stem cells especially mesenchymal stem cells (MSCs) into germ cells has recently been proposed as a new strategy for infertility treatment. The aim of this study was to evaluate the proliferation and differentiation of SSCs using their co-culture with Sertoli cells and conditioned medium (CM) from adipose tissue-derived MSCs (AD-MSCs). Testicular tissues were separated from 2-7 days old neonate Wistar Rats and after mechanical and enzymatic digestion, the SSCs and Sertoli cells were isolated and cultured in Dulbecco's modified eagle medium with 10% fetal bovine serum, 1X antibiotic, basic fibroblast growth factor, and glial cell line-derived neurotrophic factor. The cells were treated with the CM from AD-MSCs for 12 days and then the expression level of differentiation-related genes were measured. Also, the expression level of two major spermatogenic markers of DAZL and DDX4 was calculated. Scp3, Dazl, and Prm1 were significantly increased after treatment compared to the control group, whereas no significant difference was observed in Stra8 expression. The immunocytochemistry images showed that DAZL and DDX4 were positive in experimental group comparing with control. Also, western blotting revealed that both DAZL and DDX4 had higher expression in the treated group than the control group, however, no significant difference was observed. In this study, we concluded that the CM obtained from AD-MSCs can be considered as a suitable biological material to induce the differentiation in SSCs.

• 한국작물학회지
• /
• 제41권6호
• /
• pp.704-709
• /
• 1996

모시풀의 경편과 흡지의 배양을 통하여 완전한 식물체를 대량증식하기 위하여 소독방법과 생장조절제 처리 효과에 대한 기내배양을 실시하여 다음과 같은 결과를 얻었다. 1. 모시풀 경편 배양시 소독은 초음파세체기를 이용한 2% NaClO를 20분 동안 하였을때 오염률이 3.3%로 가장 낮았으며, 식물체도 79%가 생존하였고, 건실한 묘를 생산할 수 있었다. 2. 생장조절제 처리효과에서는 NAA(0.02mg/$\ell$)+ BA(1.5mg/$\ell$) + GA3(0.1mg/$\ell$) 혼합처리가 캘러스 형성이 안되고, 식물체 형성률이 96%였으며, 건실한 묘를 생산할 수 있었다. 3. 치상부위별로는 흡지보다 경편배양이, 품종별로는 개량종인 서방종보다 재래종인 보성종이 증식효률이 높았다. 4. 순화 효율은 상토 배합과 호르몬 처리에 있어서 버미큐라이트 : 모래 ：황토 =1 : 1 : 1의 배합과 NAA 1000ppm을 30분간 담근 후 이식한 순화율이 99%로서 건실하였으며, 식물체는 대부분 정상이었다.