Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Anticonvulsant potential of callus cultures of Convolvulus microphyllus Sieb.

  • Ahmad, Sayeed (Plant Tissue Culture Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard) ;
  • Zafar, Rasheed-Uz (Plant Tissue Culture Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard) ;
  • Shahid, Mohd (Department of Physiology, V. P. Chest Institute, University of Delhi)
  • Published : 2007.03.31
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Abstract

Callus cultures of Convolvulus microphyllus Sieb. was induced on Murashige and Skoog's medium supplemented with 2,4-dichloro phenoxy acetic acid, 6-benzyl adenine, indole acetic acid and kinetin (1 ppm each). Methanolic extracts of whole plant, leaf, stem and leaf and stem calli were tested for anticonvulsant activity against standard drug phenytoin using maximal electroshock model on mice. It was observed that the animals treated with methanolic extracts of stem callus, leaf callus and whole plant (200 mg/kg, oral) showed significant protection against tonic convulsions induced by transcorneal electroshock. Anticonvulsant activity of methanolic extract of stem callus was comparable to that of standard drug phenytoin.

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References

  1. Ameri A, Gleitz J, Peters T. (1997) Bicuculline-induced epileptiform activity in rat hippocampal slices: suppression by Aconitum alkaloids. Planta Med. 63, 228-232
  2. Anca JM, Lamela M, Cadavid I, Calleja JM. (1990) Effects of Himanthalia elongata on central nervous system of mice. J. Ethnopharmacol. 29, 225-231 https://doi.org/10.1016/0378-8741(90)90059-3
  3. Chadha YR. (1966) The Wealth of India, PID, CSIR, New Delhi, pp 79
  4. Chaturvedi AK, Parmar SS, Bhatnagar SCX, Misra G, Nigam SK (1974) Anticonvulsant and anti-inflammatory activity of natural plant coumarins and terpenoids. Res. Commun. Chem. Pathod. Pharmacol. 9, 11-22
  5. Chaturvedi GN, Sen SP, Sharma RK. (1966) Studies on antihypertensive effect of Shankhapushpi. J. Res. Ind. Med. 1, 57-67
  6. De Lucia R, Sertie JAA, Camargo EA, Panizza S. (1997) Pharmacological and toxicological studies on Ceniella asiatica extract. Fitoterapia 68, 413-416
  7. Gupta RC, Singh PM, Prasad GC, Udupa KN. (1981) Probable mode of action of Sankhpushpi in the management of Thyrotoxicosis. Ane. Sci. Life 1, 49-53
  8. Jaggi RK, Madan R, Singh B. (2003) Anticonvulsant potential of Holy Basil, Ocimum sanctum Linn. and cultures. Indian J. Exp. Biol. 41, 1329-1333
  9. Kapoor A, Mahor R, Vaishampayan N, Gautam N. (1981) Antifungal spectrum of some petal extracts. Geobios 8, 66-67
  10. Kitano Y, Usui C, Takasuna K, Hirohashi M, Nomura M. (1996). Increasing current electroshock seizure test, A new method for assessment of anti-and proconvulsant activities of drugs in mice. J. Pharmacol. Toxicol. Meth. 35, 25-29 https://doi.org/10.1016/1056-8719(95)00115-8
  11. Kodama T, Yamakawa T, Minoda Y. (1980). Rotenoids biosynthesis by tissue culture of Derris elliptica. Agric. Biol. Chem. 44, 2387-2390
  12. Macdonald RL, Kelly KM. (1995) Antiepileptic drug mechanism of action. Epilepsia 36, S2-S12 https://doi.org/10.1111/j.1528-1157.1995.tb05996.x
  13. Marwah R, Pal SN, Pillai KK. (1999) Effect of fluoxetine on increasing current electroshock seizure (ICES) in mice. Indian J. Pharmacol. 31, 350-353
  14. McCabe RT, Wasterlain CG, Kucharczyk N, Sofia RD, Vogel JR. (1993) Evidence for anticonvulsant and neuroprotectant action of felbamate mediated by strychnine- insensitive glycine receptors. J. Pharmacol. Exp. Ther. 264, 1248-1252
  15. Murashige T, Skoog F. (1962) A revised medium for rapid growth and bioassay for tobacco tissue cultures. Physiol. Planat. 15, 473-479 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  16. Nghyen H, Tarr KA. (1993) Comparative study on formation of flavonoids, tannins, polyphenol content in ontogenesis of Ocimum basilcum. Part II. Acta Agron. Hung. 42, 41-43
  17. PawarSA, Dhuley IN, Naik SR (2001) Neuropharmacology of an extract derived from Convolvulus microphyllus. Pharm. Biol. 39-40, 253-258 https://doi.org/10.1076/phbi.39.4.253.5920
  18. Rogawski MA, Porter RJ. (1990) Antiepileptic drugs and pharmacological mechanisms and clinical efficacy with cosideration of promising developmental stage compounds. Pharmacol. Rev. 42, 223-286
  19. Sairam K, Rao CV, Gael RK. (2001) Effect of Convolvulus pluricaulis chois on gastric ulceration and secretion in rats. Indian J. Exp. Biol. 39, 350-354
  20. Shah V, Bole PV. (1961) Botanical Identity of 'Shankhapuspi'. Indian J. Pharm. 23, 223-229
  21. Shahid M, Pillai KK, Vohra D. (2004) Reversal of phenytoin induced impairment of spontaneous alteration by piracetam in mice: Involvement of cholinergic system. Indian J. Pharmacol. 36, 1, 20-24
  22. Sharma VN, Barar FSK, Khanna NK, Mahawar MM. (1965) Some pharmacological actions of Convolvulus pluricaulis Chois: An Indian Indigenous herb. Indian J. Med. Res. 53, 871-876
  23. Shastri BN. (1952) The Wealth of India II. Raw Materials, New Delhi, CSIR, 233-234
  24. Singh RH, Sinha BN, Upadhya KN. (1979) Comparative biochemical studies on the effect of four Medhya Rasayana drugs described by Charaka on some central neurotransmitters in normal and stressed rats. J. Res. Ind. Med. 14, 7-13
  25. Subramaniam S, Rho JM, Penix L, Donevan SD, Fielding RP, Rogawski MA. (1995) Felbamate block of the N-methyl-D-aspartate receptor. J. Pharmacol, Exp. Ther. 273, 878-886
  26. White HS. (1997) New mechanisms of antiepileptic drugs. In: Epilepsies II, Boston, Butterworth Heinemann, 1-30
  27. Zafar R, Mujeeb M. (2002) Rotenoid and rutin content in callus cultures of Tephrosia purpurea. Indian J. Pharm. Sci. 64,217-221
  28. Zafar R, Ahmad S, Mujeeb M. (2005) Estimation of scopoletin in leaf and leaf callus of Convolvulus microphyllus Sieb. Indian J. Pharm. Sci. 67, 600