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THE EFFECT OF GROWTH FACTORS ON OSTEOGENIC DIFFERENTIATION OF ADIPOSE TISSUE-DERIVED STROMAL CELLS  

Kim, Uk-Kyu (Department of Oral & Maxillofacial Surgery, College of Dentistry, Pusan National Univeristy)
Choi, Yeon-Sik (Department of Oral & Maxillofacial Surgery, College of Dentistry, Pusan National Univeristy)
Jung, Jin-Sup (Department of Physiology, College of Medicine, Pusan National Univeristy)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.32, no.4, 2006 , pp. 327-333 More about this Journal
Abstract
Future cell-based therapies such as tissue engineering will benefit from a source of autogenous pluripotent stem cells. There are embryonic stem cells (ESC) and autologous adult stem cells, two general types of stem cells potentilally useful for these applications. But practical use of ESC is limited due to potential problems of cell regulation and ethical considerations. To get bone marrow stem cells is relatively burden to patients because of pain, anesthesia requirement. The ideal stem cells are required of such as the following advantages: easy to obtain, minimal patient discomfort and a capability of yielding enough cell numbers. Adipose autologus tissue taken from intraoral fatty pad or abdomen may represent such a source. Our study designed to demonstrate the ability of human adipose tissue-derived stromal cells (hATSC) from human abdominal adipose tissue diffentiating into osteocyte and adipocyte under culture in vitro conditions. As a result of experiment, we identified stromal cell derived adipose tissue has the multilineage potentiality under appropriate culture conditions. And the adipose stromal cells expressed several mesenchymal stem cell related antigen (CD29, CD44) reactions. Secondary, we compared the culture results of a group of hATSC stimulated with TGF-${\beta}$1, bFGF with a hATSC group without growth factors to confirm whether cytokines have a important role of the proliferation in osteogenic differentiation. The role of cytokines such as TGF-${\beta}$1, bFGF increased hATSC's osteogenic differentiation especially when TGF-${\beta}$1 and bFGF were used together. These results suggest that adipose stromal cells with growth factors could be efficiently available for cell-based bone regeneration.
Keywords
Human adipose tissue-derived stromal cells (hATSC); Stem cell; Osteogenic differentiation; Growth factors;
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  • Reference
1 Caplan AI: Mesenchymal stem cells. J Orthop Res 1991;9:641-650   DOI
2 Wakitani S, Saito T, Caplan AI: Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine. Muscle Nerve 1995;18:1417-1426   DOI   ScienceOn
3 Ferrari G, Cusella-De Angelis, Coletta M, Coletta M, Paolucci E, Stomaiuolo A, et al.: Muscle regeneration by bone marrow-derived myogenic progenitors. Science 1998;279:1528-1530   DOI
4 Orlic D, Kajstura J, Chimenti S, Jakoniuk J, Anderson SM, Li B, et al.: Bone marrow cells regenerate infarcted myocardium. Nature 2001;410:701-705   DOI   ScienceOn
5 Theise ND, Nimmakayalu M, Gardner R, Illei PB, Morgan G, Teperman L, et al.: Liver from bone marrow in humans. Hepatology 2000;32:11-16   DOI   ScienceOn
6 Mezey E, Chandross KJ, Harta G, Maki RA, McKercher SR: Turning blood into brain : cells bearing neuronal antigens generated in vivo from bone marrow. Science 2000;290:1770-1782
7 Choi IS: Isolation of multilineage mesenchymal stem cells from human adipose tissue and proximal femur. Pusan National University, College of Medicine, Postgraduate School, 2002
8 YI chengqing, ZHENG Qixin, GUO Xiaodong: Osteogenic Potential of Cultured Bone Marrow Stromal Cells Transfected with Transforming Growth Factor ${\beta}$1 Gene in vitro. Journal of Tongji Medical University 2001;21(2):130-133   DOI   ScienceOn
9 Andrades JA, Han B, Becerra J, Sorgente N, Hall FL, Nimni ME: A Recombinant Human TGF-${\beta}$1 Fusion Protein with Collagen-binding Domain Promotes Migration, Growth, and Differentiation of Bone Marrow Mesenchymal Cells. Experimental Cell Research 1999;250:485-498   DOI   ScienceOn
10 Quatela VC, Sherris DA, Rosier RN: The human auricular chondrocyte. Responses to growth factors. Arch Otolaryngol Head Neck Surg 1993;119(1):32-7   DOI   ScienceOn
11 M Mastrogiacomo, R Cancedda, R Quarto: Effect of different growth factors on the chondrogenic potential of human bone marrow stromal cells. Osteoarthritis and Cartilage 2001;9:S36-S40
12 Wang J, et al.: Neovascularization of ischemic myocardium by human bone marrow-derived angiblasts prevents cardiomyocyte apoptosis, reduces remodeling and improves cardiac function. Nat Med 2001;7:430-436   DOI   ScienceOn
13 Friedenstein AJ, Petrakova KV, Kurolesova AI, Frolova GP: Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantation 1968;6:230- 247   DOI   ScienceOn
14 Bruder SP, Jaiswal N, Haynesworth SE: Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation. J Cell Biochem 1997;64:278-294   DOI   ScienceOn
15 Pettersson P, Cigolini M, Sjoestroem L, Smith U, Bjomtorp P: Cells in human adipose tissue developing into adipocytes. Acta Med Scand 1984;215:447-451   DOI   ScienceOn
16 Hausman GJ, Campion DR: Histology of the stroma in developing rat subcutaneous adipose tissue. J Anim Sci 1982;55:1336-1343   DOI
17 Owen M: Marrow stromal stem cells. J Cell Sci Suppl 1988;10:63-76
18 Halvorsen YD, Franklin D, Bond AL, Hitt DC, Auchter C, Boskey AL, et al.: Extracellular matrix mineralization and osteoblast gene expression by human adipose tissue-derived stromal cells. Tissue Eng 2001;7(6):729-41   DOI   ScienceOn
19 Johnstone B, hering TM, Caplan AI, Goldber VM, Joo JU: In vitro chondrogenesis of bone marrow derived mesenchymal progenitor cells. Exp Cell Res 1998;238:265-272   DOI   ScienceOn
20 Prockop DJ: Marrow stromal cells as stem cells for non-hematopoietic tissues. Science 1997;276:71-74   DOI
21 Tsutsumi S, Shimazu A, Miyazaki K, Pan H, Koike C, Yoshida E, et al.: Retention of Multilineage Differentiation Potential of Mesenchymal Cells during Proliferation in Response to FGF. Biochemical and Biophysical Research Communications 2001;288:413-419   DOI   ScienceOn
22 Lagasse E, Connors H, Al Dhalimy, Reitsma M, Dohse M, Osborne L, et al.: Purified hematopoietic stem cells can differentiate into hepatocytes in vivo. Nat Med 2000;6:1229-1234   DOI   ScienceOn
23 Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, et al.: Multilineage potential of adult haman mesenchymal stem cells. Science 1999;284:143-147   DOI   ScienceOn
24 Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, et al.: Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 2001;7:211-28   DOI   ScienceOn
25 Pettersson P, Van R, Karlsson M, Bjorntorp P: Adipocyte presursor cells in obese and nonobese humans. Metabolism 1985; 34:808-812   DOI   ScienceOn
26 Alison MR, Poulsom R, Jeffery R, Dhillon AP, Quaglia A, Jacob J, et al.: Hepatocytes from non-hepatic adult stem cells. Nature 2000;406:257   DOI
27 Locklin RM, Williamson MC, Beresfird JN, Triffitt JT, Owen ME: In vitro effects of growth factors and dexamethasone on rat marrow stromal cell. Clin Orthop 1995;313:27
28 Gussoni E, Soneoka Y, Strickland CD, Buzney EA, Khan MK, Flint AF, et al.: Dystrophin expression in the mdx mouse restored by stem cell transplantation. Nature 1999;401,390-394
29 Brazelton TR, Rossi FM, Keshet GI, Blau HM: From marrow to brain : expression of neuronal phenotypes in adult mice. Science 2000;290:1775-1779   DOI   ScienceOn
30 Dexter TM: Stromal cell-associated hematopoiesis. J Cell Physiolo 1982;1:87-94
31 Kotev-Emeth S, Savion N, Prichen S, Pitaru S: Effect of Maturation on the Osteogenic Response of Cultured Stromal Bone Marrow Cells to Basic Fibroblast Growth Factor. Bone 2000; 27(6):777-783   DOI   ScienceOn