• Title/Summary/Keyword: stem culture

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A New Spray Chrysanthemum Cultivar, 'Dream Water' with Single Type for Cut Flower (홑꽃형 절화용 스프레이 국화 '드림워터' 육성)

  • Jung, Yun-Kyung;Lim, Jae-Wook;Kim, Sung-Kee;Lee, Young-Soon;Yu, Ye-Young
    • Horticultural Science & Technology
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    • v.30 no.2
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    • pp.220-223
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    • 2012
  • A new cultivar $Dendranthema$ $grandiflourm$ 'Dream Water' was developed at Gyeonggi-do Agricultural Research & Extension Services, Korea in 2009. The cultivar 'Dream Water' was initially derived from the cross in 2005 between 'Patra', a spray chrysanthemum cultivar with yellow single type, and 'Ruces', a spray chrysanthemum cultivar with white single type. The cultivar has single type with ivory petals. Trial evaluation was conducted from 2006 to 2009 for selection of this variety, including a shading culture in spring and a retarding culture in summer. The flowering time of 'Dream Water' was October 24th, and year-round flowering is possible by shading or lighting treatment. The diameter of the flower is 56.0 mm. Number of flowers per stem and petals per flower are 11.1 and 26.8, respectively. Its leaf color was green (Green Group 147A) and plant height was 89.7 cm. Days to flowering under the short day treatment is about 54 in spring, and diameter of flower center was 1.4 cm in the summer. The vase life was 16.7 days in autumn and consumer's preference of new spray chrysanthemum is high level than control.

In vitro shoot propagation of Ranunculus kazusensis Makino, an endangered aquatic plant (멸종위기 수생식물인 매화마름(Ranunculus kazusensis Makino)의 기내 신초 증식)

  • Park, Min Wan;Ryu, Shi Hyun;Nam, Su Hwan;Bae, Kee Hwa
    • Journal of Plant Biotechnology
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    • v.44 no.3
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    • pp.325-329
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    • 2017
  • A micropropagation method via callus for Ranunculus kazusensis Makino, an endangered species, was established. When stem segments were cultured on MS media supplemented with 1.0 mg/L IAA, NAA, IBA and 2,4-D, the highest frequency of callus induction was achieved on MS medium supplemented with 1.0 mg/L NAA. Multiple shoot per explant was obtained, the MS medium containing 1.0 mg/L BA and 0.5 mg/L NAA. Additionally, effect of activated charcoal (AC) and sucrose on shoot growth in in vitro culture were examined. The most suitable conditions for shoot growth after 4 weeks of culture were the MS medium with AC and sucrose. This in vitro propagation protocol will be valuable for conservation and mass propagation of this endangered plant.

Control of Tomato Wilt Disease by Amending pH of Nutrient Solution in Hydroponic System (토마토 수경재배에서 배양액의 pH 조절에 의한 풋마름병 방제)

  • Lee Jung-Sup;Choi Ji-Ho;Seo Sang-Tae;Han Kyoung-Suk;Park Jong-Han;Jang Han-Ik
    • Research in Plant Disease
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    • v.11 no.2
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    • pp.193-197
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    • 2005
  • The effect of pH on the survival of R. solanacearum and its transmission via roots of tomato in hydroponic culture were studied in laboratory and greenhouse. In laboratory experiment, R. solanacearum could not survive for 24h in nutrient solution with pH of $4{\cdot}0;or\;4{\cdot}5$, while 1, 14, 51 and $62\%$ of inoculum survived at pH $5{\cdot}0,\;5{\cdot}6\;and\;6{\cdot}5$, respectively. When tomato plants were inoculated with R. solanacearum through wounds on the stems, the bacteria moved downward from the inoculation site to the roots and infectious bacteria were released from the roots into the nutrient solution. Of two pH regimes tested in greenhouse nutrient-film technique(NFT) culture, the R. solanacearum population was significantly lower in pH 5.0 than in pH 6.5 in most sampling data. In treatments in which R. solanacearum was introduced by transplanting two root-inoculated plants, significantly move plants developed wilt at pH $6{\cdot}5$(34 out of 48 plants) than at pH 5.0(11 out of 48 plants). In addition, when the bacterium was introduced by transplanting two stem-inoculated plants at pH $6{\cdot}5$, seven out of 24 plants developed wilt.

Influence of Crown Gall Infection on Growth and Flowering of Rose (뿌리혹병 감염이 장미의 생육과 개화에 미치는 영향)

  • Han, Kyung-Sook;Kim, Won-Hee;Park, Jong-Han;Han, You-Kyoung;Cheong, Seung-Rong
    • Research in Plant Disease
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    • v.14 no.3
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    • pp.159-164
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    • 2008
  • Crown gall of rose (Rosa hybrida) was observed in greenhouse during 2003-2007. The average disease incidence was up to 38.1 % and was more severe in hydroponic culture as compared to soil culture. The typical gall symptom occurred mainly on the root, crown, or both, and resulted on poor rooting, growth retardation and yield loss. The reduction rate of rooting influenced by crown gall was 57.5% as compared to healthy plants on nursery stock. The location of gall formation in the plant influenced growth vigor resulting in symptoms such as poor shooting. Healthy plants produced 19.1 flowers/$m^2$, while diseased plants produced 9.5 flowers/$m^2$ during the same cultivation period. Moreover, the number of days to flowering was longer for the diseased plants than for healthy plants - 51.2 days and 39.8 days for first harvest, and 60.6 days and 52.1 days for the second harvest, respectively. Conclusively, infection on the basal stem caused serious loss of the number of shoot formation; yield loss of cut flower was 38.7% due to crown gall infection and delay of harvesting time about 8-10 days.

A Study on Food Behavior and Preference of Military Personnel (우리나라 국군 장병의 식생활 행동과 기호도에 관한 조사 연구 -현역 군 단기복무자를 중심으로-)

  • Lee, Young-Mi;Joung, Yoo-Kyung;Yang, Il-Sun;Sohn, Kyung-Hee;Moon, Soo-Jae;Kim, Kwang-Jung;Lee, Sang-Goo
    • Journal of the Korean Society of Food Culture
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    • v.5 no.4
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    • pp.463-472
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    • 1990
  • This research was carried out as a basic survey to provide more effective and improved food service for the soliders of the Armed Forces who are serving to defend the Republic of Korea subjects were selected randomly who were serving in the army for a short-term. Following is the result of a survey regarding the situation of their meals, dietary life, and their food preference of 190 kinds of foodstuffs. As a result of surveying the dietary life of the soldiers, they indicated that there was dissatisfaction in the current Food Service because it lacked tasted and the same kind of meals were being served too frequently with regard to this, many subjects expressed that they were eating out because of the quality of they get from the regular meal service the meals, that is they were eating in restaurants to resolve their dissatisfaction over the quality of the meals. It was also surveyed that in the current meals there is a need to diversify ways in preparing food, increase the number of dishes, and improve the tasts of the food by using improved cooking method. It seemed that the soldiers were satisfied in the quantitative level of the current food service but that there is a need to improve the meals in quality. Following is the result of a survey on the preference of foodstuffs. In case of rice as the stationary food, mixture of five-grain plain white cooked rice showed a higher propensity compared to plain whte boiled rice. This shows that soliders prefer various cereals in their rice. Soliders liked soup made from meat better than vegetable soup or soup made from fish. Especially there was a high performance for beef soup. Soliders liked One-dish meals such as fried rice or boiled rice with asserted mixtures, noodled, and dumplings on the whole-soliders generally liked vegetables and they liked uncooked vegetables better than cooked vegetables. Generally everyone liked beverages and there was a high preference for dessert. Among various rice cakes soliders liked songpyon(rice cake steamed on a layer of pine needles) and injolmi (cake made from glutinous rice). On the other hand, soliders did not like food prepared with fish, biscuits and candy very much. In the way of preparing food, soliders liked food that were fried in deep far rather than food cooked in water. Also they liked pot stem better than soup. As examined in the survey, those surveyed wanted improvement in the quality and diversity of the food rather than increase the number of food and quality. Therefore there is a need for diversified supply and demand of quality of meal service.

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Appropriate Root-zone Temperature Control in Perlite Bag Culture of Tomato during Winter Season (저온기 토마토 펄라이트 자루재배시 최적 근권온도 조절 방법)

  • Kim, Sung-Eun;Sim, Sang-Youn;Lee, Sang-Don;Kim, Young-Shik
    • Horticultural Science & Technology
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    • v.28 no.5
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    • pp.783-789
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    • 2010
  • The effective method for heating root-zone during winter season was studied in the aspects of growth, yield and economics for tomato ($Solanum$ $lycopersicum$) in perlite bag culture. There were four root-zone heating treatments: two hours heating from one hour before to one hour after sunrise, four hours from two hours before to two hours after sunrise, 15 hours after sunset, and no heating. The growth characteristics of the upper parts of plants were not significantly different among the treatments, but root volume increased with longer heating of the root zone. The Plant Development Index, using stem diameter and the length between growing tip and the upper flowering truss, showed relation between yield per cluster and growth pattern. The treatment heating for four hours was the most economic in terms of growth and yield of tomato.

Seed Production and Cultivation of Ecklonia stolonifera Okamura, Phaeophyta (갈조 곰피(Ecklonia stolonifera Okamura)의 종묘생산과 양성)

  • Kim, Dong-Sam;Hong, Jung-Pyo;Kim, Young-Dae;Song, Hong-In;Kim, Hyung-Geun
    • Journal of Aquaculture
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    • v.20 no.1
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    • pp.7-13
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    • 2007
  • The seed production of Ecklonia stolonifera Okamura was studied under laboratory conditions through the embryonic sporophyte stage and the field cultivation was conducted in eastern coast of Korea. The germination of zoospores occurred within 3 days and the growth of gametophytes was most rapid at $25^{\circ}C$ and $20\;{\mu}mol{\cdot}m^{-2}s^{-1}$. Sporophyte growth was highest at $20^{\circ}C$ and $20\;{\mu}mol{\cdot}m^{-2}s^{-1}$ and lowest at $25^{\circ}C$ and $80\;{\mu}mol{\cdot}m^{-2}s^{-1}$. In the nursery culture of E. stolonifera lasting for 2 weeks in January, the initial blade length of E. stolonifera (about $500\;{\mu}m$) grew to $526.3{\pm}176.0\;{\mu}m$ at water temperature of $12.05^{\circ}C$. The blade length and width reached their maxima in July, after which the ends of blade and stem began to degrade with the increase in water temperature. The degraded end of the blade started to regenerate in October, when water temperature began to decline. This species can be considered a potential candidate for aquaculture, increasing in the availability of raw material and aiding in recovery of seaweed bed.

Influence of Amount of Waste Nutrient Solution and Compost on Growth and Quality of Tomato Grown by Fertigation (폐양액 관비량과 퇴비 시용량이 토마토의 생육과 품질에 미치는 영향)

  • Zhang, Cheng-Hao;Kang, Ho-Min;Kim, Il-Seop
    • Journal of Bio-Environment Control
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    • v.18 no.2
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    • pp.119-123
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    • 2009
  • Waste nutrient solution (WNS) that composed of nutrient solution of horticultural research institute in Japan and used for culture tomato in perlite hydroponics showed $1.9{\sim}2.4$ dS/m of EC and $5.5{\sim}7.1$ of pH. Although $NH_{4}^+$-N concentration of WNS decreased remarkably, the others did not change significantly as compared with supplied solution. The growth characteristics such as plant height, stem diameter, leaf length, and leaf width were increased as amount of compost was increased in 1 L WNS supply treatment. But there was not any regular tendency on growth characteristics in 2 L/plant WNS supply treatment. The chlorophyll content of tomato leaf was highest in I L/plant WNS supply and 4,000 kg/10a compost treatment. The fresh and dry weight, and yield of tomato fruit also increased with increase of amount of WNS and compost, and they were highest in 1 L WNS supply and 4,000 kg/10a compost treatment. The sugar content of tomato fruits was not influenced by amount of WNS and compost, fructose and glucose and total sugar was highest in 2 L/plant WNS supply and 2,000 kg/10a compost treatment. Therefore fertigation culture used WNS is environmentally friendly and can guarantee yield and quality of tomato fruits and growth of tomato plants.

Plant Regeneration and Multiplication of Gentiana scabra Bunge. through Leaf and Stem Culture (용담(Gentiana scabra Bunge.)의 엽육(葉肉) 및 줄기배양에 의한 식물체 재분화와 증식(增殖))

  • Seong, Nak-Sul;Park, Chung-Heon;Lee, Seoung-Tack;Kim, Seong-Min
    • Korean Journal of Medicinal Crop Science
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    • v.1 no.2
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    • pp.129-136
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    • 1993
  • For the clonal proliferation of Gentiana scabra Bunge. which is one of the medicinaland ornamental plant, establishment multiplication of shoot through tissue culture technique and transplantation into soil were carried out. The shoot proliferation increased on the MS medium containing 0.5mg/l NAA and 0.5mg/l BAP. Optimum pH for shoot growth was pH 5.9, consequently MS medium supplemented with 2g/l activated charcoal was most effective for plant growth. There are two types of somaclonal variants, tall type was 63% and dwarf type was 37%.

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A New Spray Chrysanthemum Cultivar, 'Blue Hope' with Anemone Type and White Petals for Cut Flower (백색 아네모네형 절화용 스프레이국화 '블루호프' 육성)

  • Hwang, Ju-Chean;Chin, Young-Don;Chung, Yong-Mo;Kim, Su-Kyeong;Ro, Chi-Woong;Jeong, Byoung Ryong
    • Horticultural Science & Technology
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    • v.31 no.1
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    • pp.123-127
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    • 2013
  • A new spray chrysanthemum (Dendranthema grandiflorum) 'Blue Hope' was bred by the Flower Research Institute, Gyeongsangnam-do Agricultural Research & Extension Services in 2009. The cultivar 'Blue Hope' was initially derived from the cross in 2005 between 'Ford', a spray chrysanthemum cultivar, with white anemone type, and 'Chopin', a spray chrysanthemum cultivar with white anemone type. The cultivar has anemone type with white petals. After the evaluation of the characteristics under shade culture in summer and retarding culture in spring and consecutive selection from 2007 to 2009, 'Blue Hope' was selected finality. The natural flowering time of 'Blue Hope' was October 24th, and year-round flowering is possible by shading or lighting treatment. The growth of plant was very vigorous and response time 6.5 weeks. The diameter of flower was 4.9 cm. Number of flowers per stem was 19.1 in autumn. Days to flowering under the short day treatment was about 45 in spring and its vase life was 23.8 days in the autumn season. This cultivar was resistance white rust and consumer's preference of new spray is high level than the control.