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http://dx.doi.org/10.5010/JPB.2017.44.3.325

In vitro shoot propagation of Ranunculus kazusensis Makino, an endangered aquatic plant  

Park, Min Wan (Nakdonggang National Institute of Biological Resources)
Ryu, Shi Hyun (Nakdonggang National Institute of Biological Resources)
Nam, Su Hwan (Chollipo Arboretum)
Bae, Kee Hwa (Nakdonggang National Institute of Biological Resources)
Publication Information
Journal of Plant Biotechnology / v.44, no.3, 2017 , pp. 325-329 More about this Journal
Abstract
A micropropagation method via callus for Ranunculus kazusensis Makino, an endangered species, was established. When stem segments were cultured on MS media supplemented with 1.0 mg/L IAA, NAA, IBA and 2,4-D, the highest frequency of callus induction was achieved on MS medium supplemented with 1.0 mg/L NAA. Multiple shoot per explant was obtained, the MS medium containing 1.0 mg/L BA and 0.5 mg/L NAA. Additionally, effect of activated charcoal (AC) and sucrose on shoot growth in in vitro culture were examined. The most suitable conditions for shoot growth after 4 weeks of culture were the MS medium with AC and sucrose. This in vitro propagation protocol will be valuable for conservation and mass propagation of this endangered plant.
Keywords
Charcoal; Multiple shoot; Sucrose;
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