• 한국물환경학회지
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• 제22권1호
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• pp.172-176
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• 2006

Fe(III)-Coated Star Fish (ICSF) was prepared by reaction of calcined Star Fish (SF) with Fe(III) solution at an elevated temperature. To investigate the stability of ICSF at acidic condition, dissolution of Fe was studied at pH 2 as a function of time. Extracted iron was negligible over the entire reaction time. This stability test suggests the applicability of ICSF in the treatment of wastewater even at low pH. Adsorption capacity of Cu(II) onto SF and ICSF was investigated in a batch and a column test. In the pH-edge adsorption, adsorption of copper onto SF and ICSF was quite similar over the entire pH range due to the presence of an important amount of Fe in SF itself. From the adsorption isotherm obtained with variation of the concentration of Cu(II), ICSF showed 1.6 times greater adsorption capacity than SF. Also, ICSF showed a greater removal capacity of Cu(II) in the column test.

• 약학회지
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• 제42권4호
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• pp.388-394
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• 1998

The lectin from starfish, Asterina pectinifera, was purified and tested for its potential antitumor activity. It was shown to possess considerable toxicity toward various tumor cell lines. Concentration of Asterina pectinifera lectin (APL) at 4mg/$5{\times}10^5$ cells resulted in 28% death of Ehrlich ascites tumor cell, 40% of L929, 60% of A549, and 52% of HeLa cells after 48 hours incubation. Toxicity of APL to L929, Ehrlich ascites, A549, and HeLa cells revealed a reduction in cell viability of approximately 70% at APL concentration of 8mg/$5{\times}10^5$ cells after 48 hours incubation. Administration of APL ($100{\mu}g/day$ or $300{\mu}g/day$) inhibited the growth of Ehrlich ascites cells in vivo. Mice given only Ehrlich cells survived an average of $15{\pm}1$ (S.E.) days. Mice given Ehrlich cells and $100{\mu}g\;or\;300{\mu}g$ APL had 58% and 67% survival, respectively, after 20 days. These results suggest that APL has antitumor activity.

• Journal of Microbiology and Biotechnology
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• 제28권3호
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• pp.349-356
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• 2018

Asterias amurensis is a marine organism that causes damage to the fishing industry worldwide; however, it has been considered a promising source of functional components. The present study aimed to investigate the immune-enhancing effects of fatty acids from three organs of A. amurensis on murine macrophages (RAW 264.7 cells). A. amurensis fatty acids boosted production of immune-associated factors such as nitric oxide (NO) and prostaglandin E2 in RAW 264.7 cells. A. amurensis fatty acids also enhanced the expression of critical immune-associated genes, including iNOS, $TNF-{\alpha}$, $IL-1{\beta}$, and IL-6, as well as COX-2. Western blotting showed that A. amurensis fatty acids stimulated the $NF-{\kappa}B$ and MAPK pathways by phosphorylation of $NF-{\kappa}B$ p-65, p38, ERK1/2, and JNK. A. amurensis fatty acids from different tissues resulted in different levels of $NF-{\kappa}B$ and MAPK phosphorylation in RAW 264.7 cells. The results increase our understanding of how A. amurensis fatty acids boost immunity in a physiological system, as a potential functional material.

• 동력기계공학회지
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• 제5권2호
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• pp.22-29
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• 2001

This paper represents axial mean velocity, turbulent kinetic energy and swirl number based on momentum flux measured in the X-Y plane and Y-Z plane respectively of a cone type gas swirl burner by using X-probe from the hot-wire anemometer system. This experiment is carried out at flow rates 350 and $450{\ell}/min$ respectively, which are equivalent to the combustion air flow rate necessary for heat release 15,000 kcal/hr in gas furnace, in the test section of a subsonic wind tunnel. Axial mean velocities and turbulent kinetic energies show that their maximum values exist centering around narrow slits situated radially on the edge of and in the forefront of a burner until $X/R{\fallingdotseq}1.5$, but they have a peculiar shape like a starfish diffusing and developing into inward and outward of a burner by means of the mixing between flows ejected from narrow slits, an inclination baffle plate and swirl vanes respectively according to downstream regions. Moreover, they show a relatively large value in the inner region of 0.5$S_m$ obtained by integration of velocity profiles shows a characteristic that has an inflection point composing of the maximum and minimum value until X/R<3, but shows close agreement with the geometric swirl number after a distance of X/R=3.

• 한국수산과학회지
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• 제18권4호
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• pp.363-368
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• 1985

비닐테이프와 trapall지를 사용하여 90미의 말똥성게, Hemicentrotus pulcherrimus (A. Agassiz)에 표지를 부착시켰다. 대조군으로 표지를 부착시키지 않은 말똥성게 45미와 해적으로서 별불가사리 5미를 동시에 수용하여 1984년 6월 28일부터 9월 19일까지 84일간 실험실에서 사육시켰다. 표지부착에 따른 자연사망, 별불가사리에 의한 포식율, 표지탈락율등을 표지군별로 조사하여 대조군과 비교 분석했다. $x^2-test$의 결과 표지군과 대조군의 자연사망율은 높은 유의성이 보이나 두 표지군 사이에서는 유의성이 없었다. 표지부착여부는 해적의 포식율에 영향을 주는것으로 보이지 않으며 표지 탈락율에 대한 두 표지군 사이의 $x^2-test$ 역시 유의성이 없는 것으로 나타났다. 표지부착 직후에 일어나는 사망률(type A)은 표지 부착시 성게의 건조를 조심하면 무시될 수 있는것으로 판단된다. 표지부착상태에 따른 모든 사망율 (type B)은 비닐테이프의 경우 $47.4\%$, trapall 지의 경우 $52.6\%$였다. trapall 지는 표지가 가벼운 이점은 있으나 표지 자체가 성게에 의해 섭취될수 있고 표지의 일련번호가 쉽게 소멸될수 있는 점으로 보아 성게류의 표지방류에는 비닐테이프가 적합한 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제28권10호
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• pp.1635-1644
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• 2018

Asterias amurensis (starfish) is a marine organism that is harmful to the fishing industry, but is also a potential source of functional materials. The present study was conducted to analyze the profiles of fatty acids extracted from A. amurensis tissues and their anti-inflammatory effects on RAW264.7 macrophage cells. In different tissues, the component ratios of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids differed; particularly, polyunsaturated fatty acids such as dihomo-gamma-linolenic acid (20:3n-6) and eicosapentaenoic acid (20:5n-3) were considerably different. In lipopolysaccharide-stimulated RAW264.7 cells, fatty acids from A. amurensis skin, gonads, and digestive glands exhibited anti-inflammatory activities by reducing nitric oxide production and inducing nitric oxide synthase gene expression. Asterias amurensis fatty acids effectively suppressed the expression of inflammatory cytokines such as tumor necrosis $factor-{\alpha}$, interleukin-$1{\beta}$, and interleukin-6 in lipopolysaccharide-stimulated cells. Cyclooxygenase-2 and prostaglandin $E_2$, which are critical inflammation biomarkers, were also significantly suppressed. Furthermore, A. amurensis fatty acids reduced the phosphorylation of nuclear $factor-{\kappa}B$ p-65, p38, extracellular signal-related kinase 1/2, and c-Jun N-terminal kinase, indicating that these fatty acids ameliorated inflammation through the nuclear $factor-{\kappa}B$ and mitogen-activated protein kinase pathways. These results provide insight into the anti-inflammatory mechanism of A. amurensis fatty acids on immune cells and suggest that the species is a potential source of anti-inflammatory molecules.

• 한국식품과학회지
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• 제26권4호
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• pp.417-421
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• 1994

수산 미이용자원의 순차 용매추출물에 대한 항산화물 존재 여부 검색결과, 모자반의 경우 $IC_{50}$이 물추출구에서 $500{\mu}g/ml$, 에테르 추출구 및 메탄올 추출구에서 각각 $133,700{\mu}g/ml$로 나타났으며, 참빗풀은 에테르, 아세톤, 메탄올 추출구에서 $IC_{50}$이 각각 30, 110 및 $200{\mu}g/ml$로 나타나 대상 시료중 가장 항산화 효과가 좋았다. 미이용 수산동물 중에서는 오징어먹즙만이 물 추출구와 메탄올 추출구에서 $IC_{50}$이 각각 $390,\;100{\mu}g/ml$로 나타날 뿐 큰 항산화효과를 보이는 시료나 추출구간은 없었다. 대상 수산 미이용 동 식물에 있어서 에테르와 같은 비극성 용매 추출구에 강한 항산화 물질이 많이 용출되어 존재해 있는 것으로 나타났다.

• 한국의상디자인학회지
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• 제15권1호
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• pp.91-109
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• 2013

The main objective of this research was to understand the latest trends of natural print design through the quantitative & qualitative analysis of fashion appeared in contemporary female collections. The research criteria was defined as 3 seasons from 2011 S/S to 2012 S/S. Data collection of 726 was done through review of 'pr$\hat{e}$t-$\grave{a}$-porter Collections' of three major fashion cities; Paris, Milan and NY. Statistical analysis of frequency with chi-square test was conducted. Also qualitative interpretation of natural print design' characteristics was completed. The main findings were as follows.; The average occurrence rate of natural print design from 2011SS to 2012 SS in three collections were 6.4% in Milan 6.4%, 5.5% in Paris and 6.8% in N.Y. The five source types of natural prints in contemporary women's fashion collections were identified and the order of their appearance were as follows: flowers, plants, animals, insects & marine organisms and compound one. The plant prints were expressed by stylized or realistic touch. Flower patterns showed more variables than plants, however, there were no big difference in their image and major characteristics. The animal prints demonstrated two aspects. First one used typical animal print of fur or skin, but the other one draw the animal figure like paintings. The compound source type presented the most interesting and fresh pattern design ideas. In the insects & marine organisms, mainly butterfly and seashell & starfish, etc. appeared as real shapes or sometimes were stylized.

• Journal of Microbiology and Biotechnology
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• 제32권11호
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• pp.1382-1389
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• 2022

Asterias pectinifera, a species of starfish and cause of concern in the aquaculture industry, was recently identified as a source of non-toxic and highly water-soluble collagen peptides. In this study, we investigated the antioxidant and anti-photoaging functions of compounds formulated using collagen peptides from extracts of Asterias pectinifera and Halocynthia roretzi (AH). Our results showed that AH compounds have various skin protective functions, including antioxidant effects, determined by measuring the scavenging activity of 2,2-diphenyl-1-picrylhydrazyl radicals, as well as anti-melanogenic effects, determined by measuring tyrosinase inhibition activity. To determine whether ethosome-encapsulated AH compounds (E(AH)) exert ultraviolet (UV)-protective effects, human dermal fibroblasts or keratinocytes were incubated with E(AH) before and after exposure to UVA or UVB. E(AH) treatment led to inhibition of photoaging-induced secretion of matrix metalloproteinase-1 and interleukin-6 and -8, which are associated with inflammatory responses during UV irradiation. Finally, the antibacterial effects of AH and E(AH) were confirmed against both gram-negative and gram-positive bacteria. Our results indicate that E(AH) has the potential for use in the development of cosmetics with a range of skin protective functions.