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http://dx.doi.org/10.4014/jmb.1709.09005

Immune Enhancement Effect of Asterias amurensis Fatty Acids through NF-κB and MAPK Pathways on RAW 264.7 Cells  

Monmai, Chaiwat (Department of Marine Food Science and Technology, Gangneung-Wonju National University)
Go, Seok Hyeon (Department of Marine Food Science and Technology, Gangneung-Wonju National University)
Shin, Il-shik (Department of Marine Food Science and Technology, Gangneung-Wonju National University)
You, SangGuan (Department of Marine Food Science and Technology, Gangneung-Wonju National University)
Lee, Hyungjae (Department of Food Engineering, Dankook University)
Kang, SeokBeom (Citrus Research Station, National Institute of Horticultural & Herbal Science, RDA)
Park, Woo Jung (Department of Marine Food Science and Technology, Gangneung-Wonju National University)
Publication Information
Journal of Microbiology and Biotechnology / v.28, no.3, 2018 , pp. 349-356 More about this Journal
Abstract
Asterias amurensis is a marine organism that causes damage to the fishing industry worldwide; however, it has been considered a promising source of functional components. The present study aimed to investigate the immune-enhancing effects of fatty acids from three organs of A. amurensis on murine macrophages (RAW 264.7 cells). A. amurensis fatty acids boosted production of immune-associated factors such as nitric oxide (NO) and prostaglandin E2 in RAW 264.7 cells. A. amurensis fatty acids also enhanced the expression of critical immune-associated genes, including iNOS, $TNF-{\alpha}$, $IL-1{\beta}$, and IL-6, as well as COX-2. Western blotting showed that A. amurensis fatty acids stimulated the $NF-{\kappa}B$ and MAPK pathways by phosphorylation of $NF-{\kappa}B$ p-65, p38, ERK1/2, and JNK. A. amurensis fatty acids from different tissues resulted in different levels of $NF-{\kappa}B$ and MAPK phosphorylation in RAW 264.7 cells. The results increase our understanding of how A. amurensis fatty acids boost immunity in a physiological system, as a potential functional material.
Keywords
Immune enhancement; fatty acid; $NF-{\kappa}B$ pathway; MAPK pathway; starfish;
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