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Enzymatic Preparation and Antioxidant Activities of Protein Hydrolysates from Protaetia brevitarsis Larvae (흰점박이꽃무지 유충 단백가수분해물의 제조 및 항산화 활성)

  • Lee, Hyo-Seon;Ryu, Hee-Jeong;Song, Hyeon-Ji;Lee, Syng-Ook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.10
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    • pp.1164-1170
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    • 2017
  • Protaetia brevitarsis larvae (PBL) has recently been registered as a temporary food in Korea, and this study evaluated the application potential of PBL proteins as health functional food materials. Protein hydrolysates were prepared from PBL powder by enzymatic hydrolysis using five different proteases (alcalase, bromelain, flavourzyme, neutrase, and papain), and based on the results from the peptide content and SDS-PAGE analyses, PBL treated with alcalase or flavourzyme showed a high degree of hydrolysis (HD) value, whereas the HD value of those treated with neutrase, bromelain, or papain was minimal. The protein hydrolysates showing a high HD value were separated further into the fractions of >3 kDa and <3 kDa by a centrifugal filter system and then lyophilized, and according to the $RC_{50}$ values of the protein hydrolysates (<3 kDa) obtained from three different antioxidant analyses; the alcalase hydrolysates showed the highest antioxidant activity. Therefore, the alcalase hydrolysates were tested further for their inhibitory effects on the peroxidation of linoleic acid by measuring the thiobarbituric acid values. The results showed that the peroxidation of untreated linoleic acid increased dramatically during 6 days of incubation, but a pretreatment with the hydrolysates ($100{\sim}800{\mu}g/mL$) significantly inhibited the linoleic acid peroxidation in a dose-dependent manner for 6 days. Our current studies are focused on the identification of active peptide sequences from alcalase hydrolysates.

Effects of Total Sleep Deprivation on Fine Motor Performance (전수면박탈이 정상인의 미세운동수행 능력에 미치는 영향)

  • Lee, Heon-Jeong;Song, Hyung-Seok;Ham, Byung-Joo;Suh, Kwang-Yoon;Kim, Leen
    • Sleep Medicine and Psychophysiology
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    • v.8 no.2
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    • pp.129-137
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    • 2001
  • Objectives: The purpose of this study is to investigate the effects of 38-hour sleep deprivation on fine motor performance. The Motor Performance Series (MPS) in the Vienna Test System (computerized neurocognitive function tests) was used in this study. Methods: Twenty four subjects participated in this study. Subjects had no past history of psychiatric disorders and physical illness. Subjects had normal sleep-waking cycle without current sleep disturbances and were all right-handed (Annett's Hand Preference Questionnaire: above +9 points). To minimize the learning effects, familiarization with the Vienna Test System was performed one day before the study. Subjects were to get up at 6:00 in the morning after getting enough sleep according to his or her usual sleep-wake cycle. After awakening, subjects remained awake for 38 hours under continuous surveillance. During two consecutive study days, the subjects tested MPS at 7 AM and 7 PM each day, which means the MPS was done four times in total. During the experiment, anything that could affect the subjects' sleep such as coffee, tea, alcohol, a nap, tiring sports, and all medications were prohibited. Results: In MPS, the fine motor functions of both hands decreased after 38 hours of sleep deprivation. The decrement in motor performance was prominent in the dominant right hand. In the right hand, the total number of tapping was reduced (p<.005), and the number of misses (p<.05) and the length of misses (p<.05) of line tracking, the total length of inserting a short pin (p<.01), the total length of inserting a long pin (p<.05), and the number of misses in aiming (p<.05) increased. Such performance decrement was distinct in the morning sessions. Conclusions: These results suggest that fine motor performance decrement during sleep deprivation is predominant in the right hand, which exerts maximal motor function. The finding of decrement in motor function in tapping during sleep deprivation also suggested that the time required for exhaustion of muscles is shortened during sleep deprivation. More deterioration of motor performance was shown in the morning, which could be explained as circadian rhythm effects.

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Uplift Capacity of Wood Pile for Greenhouse Foundation (온실 기초용 나무말뚝의 인발저항력 검토)

  • Yun, Sung Wook;Choi, Man Kwon;Lee, Si Young;Yu, Chan;Yoon, Yong Cheol
    • Journal of Bio-Environment Control
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    • v.24 no.2
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    • pp.123-127
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    • 2015
  • Wood piles are rarely used in the construction of a greenhouse in Korea, but they are relatively more often used in other countries, such as the Netherlands. There are several advantages associated with wood piles: they are more cost-effective, less time-consuming, and more ecofriendly than the steel pipes (SPs) and pre-stressed highstrength (PHC) piles. However, one of the limiting conditions is that they have to be installed below the groundwater level to prevent decay. Since the groundwater levels are generally high in the reclaimed lands in Korea, wood piles are expected to be used often as reinforcements for foundations of greenhouses in these areas. In this study, we measured the uplift capacities of wood piles through in-situ uplift capacity tests with an aim to provide basic design data for wood pile foundations. In order to test their applicability, we then compared these experimentally measured ultimate uplift capacities with the ones calculated through some of the existing theoretical equations. The wood piles used in the loading tests were made of softwood (pine wood), and the tests were performed using piles with different diameters (∅25cm and ∅30cm) and embedded depths (1m, 3m, and 5m). The test results revealed that the uplift capacity of the wood piles showed a clear linearly increasing tendency in proportion to the embedded depth, with the ultimate uplift capacities for the diameters 25cm and 30cm being 9.38 and 10.56tf, respectively, at the embedded depth of 5m; thus demonstrating uplift capacities of ${\geq}9tf$. The comparison between the actually measured values of the uplift capacity and the ones calculated through equations revealed that the latter, which were obtained using the ${\alpha}$ method, were generally in an approximate agreement with the in-situ measured values.

The Effect of Oral Health Care Program Based on Motivational Interviewing (동기면담을 적용한 구강 관리 프로그램의 효과)

  • Han, Ye-Seul;Bae, Hyun-Sook;Cho, Young-Sik
    • Journal of dental hygiene science
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    • v.14 no.3
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    • pp.287-294
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    • 2014
  • The purpose of this study is to use basic data of dental hygiene curriculum with a new technique called motivational interviewing of communication skill to demonstrate the effectiveness of the method of oral health care. In this study, we performed oral health care program that has been made in dental hygiene department to university students. It was assigned to the control group and 66 and 32 experimental group based on the date of the first visit time. It conducted motivational interviewing of a total of three times in the experimental group. The analytical results of the measurements obtained in the oral examination and questionnaires. The results were as follows: The experimental group measured value was reduced after the intervention compared to before the PSR to evaluate the state of periodontal, gingival index, calculus index, plaque control record (PCR; O'Leary plaque index), simple plaque scor of Quantitative Light Induced Fluorescnece Digital measurement value (p<0.05). Experimental group decreased more and more the result of changes in the reduction of the average of the PCR. But control group was reduced to 3 weeks and increased back to the middle 16 weeks. There was also support interaction between the measurement point and the groups (p<0.05). Re-visit adherence of fit, 12.1% in the control group, the experimental group was 43.7% in the period of participation in the oral health care program. Thus, visit adherence of the experimental group was higher. In this study, a group that has motivational interviewing, It was able to confirm the improvement of oral health state. Discussion of the motivational interviewing can be applied to oral health care program.

Studies on Discrimination between Organic Rice and Non-organic Rice using Natural Abundance of Stable Isotope Nitrogen($\delta^{15}N$) (질소 안정동위원소 자연존재비($\delta^{15}N$)를 이용한 유기벼와 일반벼 판별법 탐색)

  • Lee, Hyo-Won;Lee, Sang-Mo
    • Korean Journal of Organic Agriculture
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    • v.18 no.2
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    • pp.257-269
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    • 2010
  • To investigate the possibility of discrimination between organic and non-organic rice using stable isotope nitrogen of natural abundance, organic rice of 17 samples and non-organic rice of 13 samples grown at adjoining organic rice field were collected in 2008. Rice was grinded into brown rice, milled rice and hull, and samples were analysed for nitrogen and $\delta^{15}N$ at NICEM. Authors also made inquiries about N source for both farmers who conduct organic- and non-organic rice cultivation. In order to know whether the $\delta^{15}N$ can be used in discrimination between organic and non-organic rice, discriminant analysis were made with SPSS and logistic method. 1. Organic farmers used manure, rice bran, used mushroom culture, fermented fertilizer (company products), and oil cake, but non-organic farmers applied compound fertilizer. Rice straws were remained in organic rice field while moved out in non-organic field. 2. There were difference in $\delta^{15}N$ among organic rice and its byproduct(7.760????% in hull, 6.720????% in rice), but significant difference was not found between them. And the trend was same between province. Non-organic rice showed similar results. 3. Significant difference of $\delta^{15}N$ were found between organic rice and non-organic rice (p<0.01) and between hull of organic rice and that of non-organic rice hull (p<0.05). $\delta^{15}N$ seemed to be useful criteria for discrimination of organic and non-organic rice. 4. When applied discrimination analysis of SPSS and logistic, there were significant difference between organic rice, non-organic rice and its byproducts except brown rice and hull in SPSS method. Hull can be used as the most useful component for unknown sample prediction with 83.3% probability.

Associations between Vasomotor Symptoms and Depression, Stress and Quality of Life in Midlife Women (중년여성에서의 혈관운동증상과 우울, 스트레스, 삶의 질과의 상관성)

  • Nam, Yoon-Min;Joe, Sook-Haeng;Kwon, Eun-Joo;Ham, Byung-Joo;Han, Chang-Su;Ko, Young-Hoon
    • Korean Journal of Psychosomatic Medicine
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    • v.24 no.2
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    • pp.146-156
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    • 2016
  • Objectives : The aim of this study was to evaluate the associations between vasomotor symptoms and factors such as sociodemographics, health behaviors, medical condition, depression, stress, anxiety, attitude toward menopause, and quality of life. Methods : We conducted a cross-sectional study in peri- and post-menopausal women enrolled by the Korean Association of Health Promotion. Subjects submitted self-report questionnaires about vasomotor symptoms and other clinical symptoms. Associations between vasomotor symptoms and clinical variables were analyzed using stepwise multiple regression analyses. Results : 1951 women completed self-report questionnaires and 1022 women were enrolled in the study. The prevalence of vasomotor symptoms in peri- and post-menopausal women was 63.9%. Variables showing significant differences between subjects with vasomotor symptoms and subjects without them were score of Beck Depression Inventory, Brief Encounter Psychosocial Instrument-Korean Version, proportions of clinically significant depression(Beck Depression Inventory ${\geq}16$), Menopausal rating scale, attitude towards menopause, the 4 subscales of World Health Organization Quality of Life-BREF(Physical health, psychological, social relationships, environment), and a History of Premenstrual syndrome/Premenstrual dysphoric disorder. Stepwise multiple regression analyses indicated that Beck Depression Inventory, Brief Encounter Psychosocial Instrument-Korean Version, Menopausal Rating Scale, and the Psychological subscale of World Health Organization Quality of Life -BREF show associations with vasomotor symptoms. Conclusions : Menopausal vasomotor symptoms are associated with various psychological factors, especially with depression. Midlife women suffering vasomotor symptoms should therefore be screened for depression. Future prospective studies where clinical subjects are diagnosed using structured interviews, focusing on the causal relationship between depression and vasomotor symptoms are necessary.

A Study on the Influence of the Quality of the Care Service of the Caregivers in a Nursing Hospital for the Elderly in the Intent of Reuse: Focusing on Chinese-Korean Caregivers (노인요양병원 간병인의 돌봄서비스 질이 재이용의도에 미치는 영향 연구: 중국동포 간병인을 중심으로)

  • Song, In Sik
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.5
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    • pp.456-467
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    • 2019
  • The rapid aging of South Korea is causing difficulties in meeting the man-power demands for the increasing number of elderly nursing hospitals. To cope with this gap in supply and demand, more foreign workers are now being hired to provide elderly care services. The purpose of this study is to examine the influence of the quality of the care services provided by foreign caregivers in nursing hospitals for the elderly. For this purpose, the researcher surveyed 249 senior citizens who are receiving care services from Chinese-Korean caregivers in six elderly nursing hospitals in Seoul and Gyeonggi region. The data collected from the survey were analyzed through SPSS and AMOS. The result of the analyses showed that, first, the quality factors of the care services of the caregivers at elderly nursing hospitals, such as reliability, responsibility, empathy, formality, and expertise, all turned out to have a positive correlation with the trust in, satisfaction with, and intent to revisit the institute. Second, thefluencing factors for the qualities of the care services by Chinese-Korean care givers in elderly nursing hospitals included responsiveness, materiality, and expertise, while the factors that influenced satisfaction with significance included trust, materiality, and expertise. Also, the trust in and satisfaction with the institution both influenced the intent of revisit in a positive manner. Third, the indirect effect of trust in the relationship between the quality of the care services by the Chinese-Korean care givers and satisfaction, appeared in all independent variable except for responsiveness, which was a factor of the quality of the care services. And, it was also shown that the satisfaction level had an indirect effect in the relationship between trust and revisiting intent. The result of this study implicates that, in order to cause the quality of the care service by the Chinese-Korean care givers in elderly nursing hospitals to increase the revisit rate, it would be necessary to provide a strategy to increase the levels of trust and satisfaction through a higher quality level of care services.

Analysis of the Entrance Surface Dose (ESD) and Contrast Usage of Trance Femoral Cerebral Angiography (TFCA) and Cerebral Computed Tomographic Angiography (CCTA) for Cerebrovascular Disease Examining (뇌혈관 질환 검사를 위한 뇌혈관 조영술(TFCA)과 뇌혈관 전산화 단층 촬영 검사(CCTA)의 입사표면선량(ESD) 및 조영제 사용량 분석)

  • Seo, Young-Hyun;Hong, Cheon-Gi;Song, Jong-Nam
    • Journal of the Korean Society of Radiology
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    • v.13 no.4
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    • pp.495-502
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    • 2019
  • A typical cerebrovascular disease among cerebrovascular diseases is vascular diseases such as cerebral infarction, cerebral hemorrhage, cerebral aneurysm, cerebrovascular stenosis. If the disease occurs and causes cerebral damage, it may be difficult to recover completely. So that, Must continue to perform health care through examination early. In particular, Because most cerebrovascular disease examining use radiation equipment and Thus this study was to find out how to select about the optimal examining method and X-ray dose decrease method among different examining method though comparison and analysis for the entrance surface dose (ESD) on cerebrovascular examining with Trance Femoral Cerebral angiography (TFCA) and Cerebral Computed Tomographic Angiography (CCTA). Also, want to find out how to select about the optimal examining method for worried patient that contrast medium side effect though measuring and evaluating for contrast usage. Data were collected from 70 patients (43 males and 27 females) who underwent CCTA at Yeosoo region hospital from June 2018 to December 2018 and 61 patients (34 males and 27 females) who underwent TFCA at Pyeongtaek region hospital from June 2018 to November 2018. ESD analysis method collected retrospective data though M-view and PACS PLUS program, Used contrast usage measuring method did reality measuring method. In the analysis using SPSS, the ESD of TFCA was $245.74{\pm}71.91$, which was $32.05{\pm}7.74$ lower than the dose of $277.79{\pm}79.65$ of CCTA ESD, and statistically significant at t = 3.249, p = 0.017 (p<0.05). As a result of the comparison of the total amount of contrast agent, the mean contrast agent used in TFCA was $55.05{\pm}17.68ml$, which was about 14.95 smaller than the amount of contrast agent used in CCTA, and statistically significant t = -4.548, p<0.001. In conclusion, the ESD of TFCA was statistically significantly lower than that of CCTA, and also the used contrast usage was significantly tiny than that of CCTA. Therefore, Select the method to increase the utilization of TFCA for cerebral disease examining, we can consider X-ray dose decrease method at the same time as to decrease side effect of contrast medium.

A Study on the Effect of Person-Job Fit and Organizational Justice Recognition on the Job Competency of Small and Medium Enterprises Workers (중소기업 종사자들의 직무 적합성과 조직 공정성 인식이 직무역량에 미치는 영향에 관한 연구)

  • Jung, Hwa;Ha, Kyu Soo
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.14 no.3
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    • pp.73-84
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    • 2019
  • Despite decades of work experience, workers at small- and medium-sized enterprises(SME) here have yet to make inroads into the self-employed sector that utilizes the job competency they have accumulated at work after retirement. Unlike large companies, SME do not have a proper system for improving the long-term job competency of their employees as they focus on their immediate performance. It is necessary to analyse the independent variables affecting the job competency of employees of SME to derive practical implications for the personnel of SME. In the preceding studies, there are independent variable analyses that affect job competency in specialized industries, such as health care, public officials and IT, but the analysis of workers at SME is insufficient. This study set the person-job fit and organizational justice based on the prior studies of the independent variables that affect the job competency of SME general workers as a dependent variable. The sub-variables of each variable derived knowledge, skills, experience, and desire for person-job fit, and distribution, procedural and deployment justice for organizational justice, respectively. The survey of employees of SME in Korea was conducted from February to March 2019 by Likert 5 scales, and the survey was retrieved from 323 people and analyzed in a demonstration using the SPSS and AMOS statistics package. Among the four sub-independent variables of person-job fit, knowledge, skills and experience were shown to have a significant impact on the job competency, and desire was not shown to be so. Among the three sub-independent variables of organizational justice, deployment justice has a significant impact on job competency, but distribution and procedural justices have not. Personnel managers of SME need to improve the job competency of their employees by appropriately utilizing independent variables such as knowledge, skills, experience and deployment at each stage, including recruitment, deployment, and promotion. Future job competency modeling studies are needed to overcome the limitations of this study, which fails to objectively measure job competency.

A Study on the Decisive Factors Influencing the Career Preparation Activities of North Korean Adolescent Defector (탈북 청소년 진로준비행동에 영향을 미치는 결정요인에 관한 연구)

  • Cho, Hyun-Seob;Chae, Kyung-Hee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.6
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    • pp.501-513
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    • 2019
  • In this study, which is based on the findings of the preceding studies, the researchers determined social support and Career Decision Self-efficacy as the key influencing variables for the successful Career Preparation Activities, to analyze the relationship between Career Preparation Activities and these variables, while identifying the mediating effect of Career Decision Self-efficacy in the relationship between social support and Career Preparation Activities. In addition, there is an objective to provide effective career counseling materials. For this study, a total of 174 North Korean adolescent defectors, who were in their middle and high schools, were interviewed and the resultant data were analyzed the data based on the questionnaire data of 204 copies from March 12 to 19, 2019. For the analysis of the data, SPSS and AMOS statistical suites were used to verify the hypothesis. The result of the analysis showed that, first, in the relationship between the social support, Career Decision Self-efficacy, and Career Preparation Activities of the North Korean adolescent defectors social support, Career Decision Self-efficacy, and Career Preparation Activities were all positively related. Especially, Career Decision Self-efficacy has been identified as the variable that is highly related to Career Preparation Activities. Second, rather than social support(${\beta}=.107$), Career Decision Self-efficacy(${\beta}=.388$) turned out to have more profound direct influence on Career Preparation Activities. Third, social support did not influence Career Preparation Activities directly(${\beta}=.107$, p>.05) but completely intermediated Career Decision Self-efficacy to influence Career Preparation Activities(Indirect effect=.307, p<.05, Z-value=2.924, p<.01). The findings of this study show that, in order to enhance the Career Preparation Activities of the North Korean adolescent defectors, it is necessary to examine how they perceive the emotional, informational, material, and evaluative supports from the surrounding environment are perceived and identify the abilities, values, and career desires of themselves through a behavioral planning that can establish a professional and value system in accordance with them.