• Title/Summary/Keyword: spectrofluorimetry

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A Study on the Analysis of Histamine by Spectrofluorimetry (형광측정법을 이용한 히스타민 분석방법 개발 연구)

  • Rhee, Jae Seong;Kim, Young Sang;Cha, Ki Suk;Rhee, Hee Jung;Hong, Chein Soo;Nam, Hae Won;Myung, Choon Ok;Lee, Ki Wan;Lee, Yang Cha
    • Analytical Science and Technology
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    • v.10 no.1
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    • pp.43-52
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    • 1997
  • The application of high performance liquid chromatogaphy(HPLC) with fluorimetric detection for the measurement of histamine in biological fluids has been developed recently. Because of time consumption during measurement and difficulties in controling optimum operating condition for HPLC system, spectrofluorimetry was adopted to detect histamine. Detection limit by spectrofluorimetry was $3.0{\times}10^{-2}{\mu}g/mL$ wi th R. S. D. 3.74%~16.2% on standard solution. The relative ratio of histmine levels between the result from HPLC-fluorimetry and that from spectrofluorimetry ranged from 1.5 to 3.5. The recoveries of each samples obtained from standard addition method by spctrofluorimetry have shown 100%~125%.

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Determination of Polycyclic Aromatic Hydrocarbons (PAHs) in Atmospheric Samples by Synchronous 2nd Derivate Spectrofluorimetry (동시형광 분광광도법에 의한 대기 시료 중의 다환방향족 탄화수소(PAHs)의 분석)

  • 유광식;정선이;정지영
    • Journal of Korean Society for Atmospheric Environment
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    • v.20 no.1
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    • pp.129-138
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    • 2004
  • Determination of some PAHs in ambient air at Ulsan have been carried out by collection of the components into n-hexane followed by synchronous spectrofluorimetric technique. 10 PAHs, such as acenaphthene (Ace), anthracene (Anth), benz[a]anthracene (BaA), benzo[b]fluoranthene (BbFt), benzo[k]fluoranthene (BkFt) benzo[a]pyrene (BaP), chrysene (Chry), phenanthrene (Phen), fluoranthene (Ft), perlyrene (Per), and pyrene (Pyr) in air samples were able to determine separately by synchronous spectrofluorimetry. Calibration curves for those components were linear for the concentration range of 0.2∼166ppb PAHs with the correlation factor of 0.9985∼0.9999. The predominant contribution was phenanthrene which was included 36.9∼85.1% to the overall level of the 10 PAHs in some areas. Also benzo[a]pyrene which was known to carcinogenicity was detected from 6.4 to 55.8ng/㎥, benzo[a]anthracene of some areas was contained from 21.9∼153ng/㎥.

Simultaneous Determination of Polycyclic Aromatic Hydrocarbons by Synchronous Spectrofluorimetry (형광분광법에 의한 방향족 탄화수소의 동시정량)

  • 이상학;손범목
    • Journal of Environmental Science International
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    • v.12 no.1
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    • pp.87-92
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    • 2003
  • Synchronous spectrofluorimetry was carried out for the simultaneous determination of various polycyclic aromatic hydrocarbons(PAHs) in aqueous solution by fluorescence spectrometry have been studied. The optimal wavelength interval(${\triangle}{\lambda}$) for synchronous spectra of acenaphthene, anthracene, benzo[a]anthracene, fluorene and pyrene were investigated in the presence of surfactants. The great enhancement of the fluorescence of these PAHs in Triton X-100 was obtained and optimal wavelength was 50 nm. The calibration curves in synthetic mixture solution of 5 PAHs were linear over the range from $1.0{\times}10^{-8}M$ to $1.0{\tiems}10^{-4}M$. Under the optimal experimental conditions, the detection limits were $4.9{\tiems}10^{-9}M$,\;7.0{\times}10^{-9}M,\;4.7{\tiems}10^{-9}M,\;1.6{\tiems}10^{-9}M$ and $3.2{\tiems}10^{-9}M$ for acenaphthene, anthracene, benzo[a]anthracene, fluorene and pyrene, respectively.

Determination of Mefenamic Acid in Human Urine by Means of Two Spectroscopic Methods by Using Cloud Point Extraction Methodology as a Tool for Treatment of Samples

  • Tabrizi, Ahad Bavili
    • Bulletin of the Korean Chemical Society
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    • v.27 no.11
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    • pp.1780-1784
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    • 2006
  • Cloud point extraction was used to extract mefenamic acid (MF) from human urine, and spectrofluorimetry and spectrophotometry were used to analyze extracted MF. The variables affecting extraction and phase separation, i.e. HCl and Triton X-114 concentration, temperature and time of equilibration, were optimized. Under the experimental conditions used the limit of detection for extraction of 25 mL of sample was 0.006 and 0.045 mg $L^{-1}$, with relative standard deviations of 2.52 and 1.45% (n = 5) for spectrofluorimetric or spectrophotometric methods, respectively. Good recoveries in the range of 95-107% were obtained for spiked samples. The proposed methods were applied to the determination of MF in human urine.

Microanalysis of Vecuronium Bromide in Biological Fluids by Spectrofluorimetry and HPLC-Fluorescence Detection (형광광도법 및 HPLC-형광검출법에 의한 생체시료 중의 Vecuronium bromide의 미량분석)

  • Ko, Yong-Seok;Han, Sang-Soo;Shin, Tae-Yong;An, Nyeon-Hyoung;Ock, Chi-Wan
    • Analytical Science and Technology
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    • v.7 no.3
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    • pp.413-419
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    • 1994
  • The determination of the neuromuscular blocking agents vecuronium bromide(VeBr) in biological fluids has been investigated. The method depends on the formation of insoluble red complex between vecuronium bromide and rose bengal in aqueous layer. The amount of vecuronium bromide was calculated from that of extracted rose bengal which was determined by spectrofluorimetry or HPLC/fluorescence detection method. It was possible to analyze VeBr in the range of $2{\sim}32{\mu}g/ml$(r=0.998 for water soln., 0.999 for urine, 0.996 for plasma). This method was applied to the analysis of VeBr in biological fluids, urine and plasma.

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Fluorescence of Styrene and Acrylic Acid Copolymers Containing Eu$^{3+}$ in Tetrahydrofuran Solution

  • Lee, Jong-Gyu;Kim, Kang-Jin
    • Bulletin of the Korean Chemical Society
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    • v.6 no.3
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    • pp.164-168
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    • 1985
  • The fluorescence emission of polystyrene-acrylic acid copolymers containing $Eu^{3+}$ in tetrahydrofuran solution was investigated by spectrofluorimetry. The excimer emission increased linearly with the polymer concentration up to approximately $5{\times}10^{-3}$M. Benzene and toluene collisionally quenched the excimer fluorescence and thier rate constants of quenching were determined. Quenching efficiencies decreased in the order: naphthalene > toluene > benzene. Analyses of Rayleigh scattering and europium emission showed no measurable structural changes observed under the experimental conditions.

A Cloud Point Extraction-Spectrofluorimetric Method for Determination of Thiamine in Urine

  • Tabrizi, Ahad Bavili
    • Bulletin of the Korean Chemical Society
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    • v.27 no.10
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    • pp.1604-1608
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    • 2006
  • A simple and efficient cloud point extraction-spectrofluorimetric method for the determination of thiamine in human urine is proposed. The procedure is based on the oxidation of thiamine with ferricyanide to form thiochrome, its extraction to Triton X-114 micelles and spectrofluorimetric determination. The variables affecting oxidation of thiamine, extraction and phase separation were studied and optimized. Under the experimental conditions used, the calibration graphs were linear over the range 2.5-1000 ng $mL^{-1}$. The limit of detection was 0.78 ng $mL^{-1}$ of thiamine and the relative standard deviation for 5 replicate determinations of thiamine at 400 ng $mL^{-1}$ concentration level was 2.42%. Average recoveries between 93-107% were obtained for spiked samples. The proposed method was applied to the determination of thiamine in human urine.

Determination of Rh(III) by Spectrofluorimetry Using Oxidation Reaction of Nile Blue (Nile Blue의 산화반응을 이용한 Rh(III)의 형광분광법적 정량)

  • Lee, Sang Hak;Lee, Myeong
    • Journal of the Korean Chemical Society
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    • v.45 no.1
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    • pp.25-30
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    • 2001
  • A selective kinetic fluorimetric method for the determination of trace rhodium(Ⅲ), based on the catalytic effect of rhodium(Ⅲ) on the oxidation of nile blue by periodate have been studied. The effects of pH and concentrations of nile blue, sodium periodate, trioctyl phosphine oxide(TOPO) and temperature were investigated. The calibration curve for rhodium(Ⅲ) ion was linear over the range from 100 ng/mL to 0.1 ng/mL and the detection limit was 0.01 ng/mL under the optimal experimental conditions. Effects of interferences from several cations and anions for the determination of rhodium(Ⅲ) were also investigated.

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A Simple Spectrofluorimetric Method for Determination of Mefenamic Acid in Pharmaceutical Preparation and Urine

  • Tabrizi, Ahad Bavili
    • Bulletin of the Korean Chemical Society
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    • v.27 no.8
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    • pp.1199-1202
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    • 2006
  • A simple, sensitive and rapid spectrofluorimetric method was developed for determination of mefenamic acid in pharmaceutical preparation and human urine. The procedure is based on the oxidation of mefenamic acid with cerium (IV) to produce cerium (III), and its fluorescence was monitored at 354 nm after excitation at 255 nm. The variables affecting oxidation of drug were studied and optimized. Under the experimental conditions used, the calibration graphs were linear over the range 0.03-1.5 mg $L^{-1}$. The limit of detection was 0.009 mg $L^{-1}$ and the relative standard deviation for 5 replicate determinations of mefenamic acid at 1.0 mg $L^{-1}$ concentration level was 1.72%. Good recoveries in the range of 102-107 and 102-109% were obtained for pharmaceutical preparation and human urine, respectively. The proposed method was applied to the determination of MF in one pharmaceutical preparation and human urine. The amounts of mefenamic acid found are very similar to those obtained by a standard method.