Browse > Article
http://dx.doi.org/10.5012/bkcs.2006.27.10.1604

A Cloud Point Extraction-Spectrofluorimetric Method for Determination of Thiamine in Urine  

Tabrizi, Ahad Bavili (Department of Medicinal Chemistry, Faculty of Pharmacy, Tabriz University of Medical Sciences)
Publication Information
Bulletin of the Korean Chemical Society / v.27, no.10, 2006 , pp. 1604-1608 More about this Journal
Abstract
A simple and efficient cloud point extraction-spectrofluorimetric method for the determination of thiamine in human urine is proposed. The procedure is based on the oxidation of thiamine with ferricyanide to form thiochrome, its extraction to Triton X-114 micelles and spectrofluorimetric determination. The variables affecting oxidation of thiamine, extraction and phase separation were studied and optimized. Under the experimental conditions used, the calibration graphs were linear over the range 2.5-1000 ng $mL^{-1}$. The limit of detection was 0.78 ng $mL^{-1}$ of thiamine and the relative standard deviation for 5 replicate determinations of thiamine at 400 ng $mL^{-1}$ concentration level was 2.42%. Average recoveries between 93-107% were obtained for spiked samples. The proposed method was applied to the determination of thiamine in human urine.
Keywords
Thiamine; Cloud point extraction; Spectrofluorimetry; Urine;
Citations & Related Records

Times Cited By Web Of Science : 5  (Related Records In Web of Science)
Times Cited By SCOPUS : 5
연도 인용수 순위
1 Du, J.; Li, Y.; Lu, J. Talanta 2002, 57, 661   DOI   ScienceOn
2 Vinas, P.; López-Erroz, C.; Balsalobre, N.; Hernandez-Cordoba, M. J. Chromatogr. B 2001, 757, 301   DOI   ScienceOn
3 Batifoulier, F.; Verny, M.-A.; Besson, C.; Demigné, C.; Remesy, C. J. Chromatogr. B 2005, 816, 67   DOI   ScienceOn
4 Bohrer, D.; Cícero do Nascimento, P.; Ramirez, A. G.; Karlo, J.; Mendonça, A.; de Carvalho, L. M.; Cristina, G.; Pomblum, S. Microchem. J. 2004, 78, 71   DOI   ScienceOn
5 Lynch, P. L. M.; Trimble, E. R.; Young, I. S. J. Chromatogr. B 1997, 701, 120   DOI   ScienceOn
6 Vinas, P.; Lopez-Erroz, C.; Balsalobre, N.; Hernandez-Cordoba, M. J. Chromatogr. A 2003, 1007, 77   DOI   ScienceOn
7 Vinas, P.; Lopez-Erroz, C.; Cerdan, F. J.; Campillo, N.; Hernandez- Cordoba, M. Mikrochim. Acta 2000, 134, 83   DOI   ScienceOn
8 Shi, Z.; He, J.; Chang, W. Talanta 2004, 64, 401   DOI   ScienceOn
9 Delgado, B.; Pino, V.; Ayala, J. H.; González, V.; Afonso, A. M. Anal. Chim. Acta 2004, 518, 165   DOI   ScienceOn
10 Shemirani, F.; Baghdadi, M.; Ramezani, M.; Reza Jamali, M. Anal. Chim. Acta 2005, 534, 163   DOI   ScienceOn
11 Bavili Tabrizi, A. Food Chem. 2007, 100, 1698   DOI   ScienceOn
12 Manzoori, J. L.; Bavili-Tabrizi, A. Michrochim. Acta 2003, 141, 201   DOI
13 Rukhadze, M. D.; Tsagareli, S. K.; Sidamonidze, N. S.; Meyer, V. R. Anal. Biochem. 2000, 287, 279   DOI   ScienceOn
14 Ohashi, A.; Ogiwara, M.; Ikeda, R.; Okada, H.; Ohashi, K. Anal. Sci. 2004, 20, 1353   DOI   ScienceOn
15 Perez-Ruiz, T.; Martinez-Lozano, C.; Tomas, V.; Ibarra, I. Talanta 1992, 39, 907   DOI   ScienceOn
16 Shen, J.; Shao, X. Anal. Chim. Acta 2006, 561, 83   DOI   ScienceOn
17 Giokas, D. L.; Sakkas, V. A.; Albanis, T. A.; Lampropoulou, D. A. J. Chromatogr. A 2005, 1077, 19   DOI   ScienceOn
18 Aboul-Kasim, E. J. Pharm. Biomed. Anal. 2000, 22, 1047   DOI   ScienceOn
19 Song, Z.; Hou, S. J. Pharm. Biomed. Anal. 2002, 28, 683   DOI   ScienceOn
20 Rocha, F. R. P.; Fatibello Filho, O.; Reis, B. F. Talanta 2003, 59, 191   DOI   ScienceOn
21 Paleologos, E. K.; Giokas, D. L.; Karayannis, M. I. Trends Anal. Chem. 2005, 24, 426   DOI   ScienceOn
22 Ortega Barrales, P.; Dominguez Vidal, A.; Fernandez de Cordova, M. L.; Molina Díaz, A. J. Pharm. Biomed. Anal. 2001, 25, 619   DOI   ScienceOn
23 Oliveira Costa-Neto, C.; Vicente Pereira, A.; Aniceto, C.; Fatibello-Filho, O. Talanta 1999, 48, 659   DOI   ScienceOn
24 Burguera, J. L.; Burguera, M. Talanta 2004, 64, 1099   DOI   ScienceOn
25 Sosa Ferrera, Z.; Padron Sanz, C.; Mahugo Santana, C.; Santana Rodríguez, J. J. Trends Anal. Chem. in press
26 Pérez-Ruiz, T.; Martínez-Lozano, C.; Sanz, A.; Guillén, A. J. Pharm. Biomed. Anal. 2004, 34, 551   DOI   ScienceOn
27 Mrestani, Y.; Neubert, R. H. H. J. Chromatogr. A 2000, 871, 351   DOI   ScienceOn
28 Lynch, P. L. M.; Young, I. S. J. Chromatogr. A 2000, 881, 267   DOI   ScienceOn
29 Lopez-Flores, J.; Fernandez-De Cordova, M. L.; Molina-Diaz, A. Anal. Chim. Acta 2005, 535, 161   DOI   ScienceOn
30 Losa, R.; Sierra, M. I.; Fernandez, A.; Blanco, D.; Buesa, J. M. J. Pharm. Biomed. Anal. 2005, 37, 1025   DOI   ScienceOn