• Title/Summary/Keyword: simple procedure

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Analysis of dioxin-like PCBs in Soil samples (토양 중 dioxin-like PCBs의 분석)

  • Kim, Kyeo Keun;Shin, Sun Kyoung;Kim, Tae Seung;Chang, JunYoung;Kim, Jeong-Gyu
    • Analytical Science and Technology
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    • v.15 no.5
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    • pp.466-474
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    • 2002
  • The analytical method of 14 kinds of coplanar - PCBs was established and applied the soil sample. The three kinds of extraction solvents (toluene, acetone: n-hexane, dichloromethane) were selected to apply the soil sample. The silica gel, florisil and alumina column cleanup also performed to compare the elution recovery. The average recovery of selected solvents in soil A, B and C was surveyed the 84.25%, 56.09% and 44.69% for toluene, 52.56%, 81.42% and 58.53% for acetone : n - hexane and 55.94%, 71.33% and 61.05% for dichloromethane. The average recovery is represented 49.99% for silica gel (n - hexane 100 mL), 69.65% for florisil (6% ether/n - hexane 100 mL), and 65.23% for alumina (2% DCM : n - hexane 100 mL, 50% DCM: n-hexane 150 mL). In silica gel (n - hexane) and florisil (6% ether : n - hexane) cleanup, the 14 kinds of coplanar PCBs eluted until 40 mL. In the silica gel and florisil columns cleanup, the amounts of elution solvent can be reduced from these results, but the researcher has to confirm the elution amounts before performing the experiments. In alumina cleanup process, the result was obtained to the 100 mL of elution solvents (2% DCM: n-hexane 100 mL and 50% DCM: n-hexane 40 mL), therefore the change of elution solvent is necessary to develop the simple procedure.

Giant Neurofibroma on Both Buttocks (양측 엉덩이의 거대 신경섬유종)

  • Kim, Ji Hoon;Burm, Jin Sik;Kim, Yang Woo;Kang, So Ra;Kim, Hyoung Kyoung
    • Archives of Plastic Surgery
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    • v.36 no.4
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    • pp.512-515
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    • 2009
  • Purpose: Neurofibromatosis(NF) is an autosomal - dominant systemic disease. Up to fifty percent of patients with NF are reported to have concomitant vascular abnormalities. In the resection of a larger NF, the risk of uncontrolled hemorrhage is much higher due to the difficulty of hemostasis of large vessels within the tumor. We ligated the base of the giant NF with a simple loop - shaped ligation before removal of the giant NF in both buttocks. And then we could successfully reduce the amount of hemorrhage during the operation. Methods: A 46 - year - old female patient presented for giant masses of both gluteal area, which has been growing slowly for the last ten years. Each mass was about $30{\times}20cm$ in size. After designing the elliptical resection margin, we tightened the tumor base by using continuous loop - shaped suture ligation(weaving the thread up and down in a loop - shaped pattern, leaving a space of 2 cm between each loop) with a straight needle and prolene 2 - 0. After skin incision, we proceeded the dissection toward the central and inferior side of the mass obliquely while we avoided breaking large vascular sinuses. We resected the tumor in a wedged - shape. Subcutaneous tissue was sutured layer by layer and skin was closed by vertical mattress and interrupted suture. The loop - shaped ligation of the base was removed and compressive dressing was done with gauzes and elastic bandages. Results: Postoperative complications such as infection, hemorrhage, hematoma, and dehiscense did not occur. Perioperatively the patient was sufficiently transfused with five units of blood and two units of fresh frozen plasma. During the subsequent 1 year follow - up, the functional and cosmetic result was excellent. Conclusion: A continuous loop - shaped suture ligation procedure along the base of the giant NF effectively reduced the amount of hemorrhage during the operation, made dissection and ligation of vessels easily and quickly, and shorten the operating time and postoperative recovery time.

A Numerical Study on Load Distribution Factors for Simplified Composite H-Beam Panel Bridges (강합성 초간편 H형강 교량의 하중분배계수에 관한 해석적 연구)

  • Park, Jong Sup;Kim, Jae Heung
    • Journal of Korean Society of Steel Construction
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    • v.21 no.3
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    • pp.221-232
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    • 2009
  • The load distribution factor (LDF) values of simplified composite H beam panel bridges (SCHPBs) that were subjected to one lane and two lane loads were investigated using three dimensional finite element analyses with the computer program ABAQUS (2007). This study considered some design parameters such as the slab thickness, the steel plate thickness, the span length, and the continuity of the SCHPBs in the development of new LDFs. The distribution values that were obtained from these analyses were compared with those from the AASHTO Standard, LRFD, and the equations presented by Tarhini and Frederick, Huo et al., Back and Shin, and Cai. The AASHTO Standard distribution factors for SCHPBs were found to be very conservative. Sometimes, the distribution values from the finite element analyses for interior girders were similar to the results of the AASHTO LRFD, whereas the values for exterior girders were conservative in most cases. The new distribution values that were presented in this study produced LDFs that are more conservative than those from the finite element method. For the simple application of the design to SCHPBs, bridge engineers can use 0.42 for the interior girder and 0.32 for the exterior girder. The proposed values improve the current design procedure for the LDF problem and increase SCHPB design efficiency.

Availability of the Optic Nerve Sheath Diameter Measured by Using Ultrasonography as a Secondary Survey for Patient with Head Injuries in the Emergency Department (응급실에 내원한 두부외상환자의 2차 평가로써 초음파를 이용한 시각신경집 지름 측정은 유용한가?)

  • Lee, Dong Wook;Lee, Jung Won;Park, Sae Hoon;Park, Ihl Sung;Lee, Hyun Jung;Yoo, Byeong Dae;Moon, Hyung Jun
    • Journal of Trauma and Injury
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    • v.26 no.3
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    • pp.104-110
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    • 2013
  • Purpose: Bedside ultrasonography is available in most emergency departments, and detecting the intracranial pressure is elevated is critical. Our objective is to evaluate the availability of bedside optic nerve ultrasound (ONUS) as a secondary survey for patients with head injuries in the emergency department (ED). Methods: From September, 2012, to March, 2013, we performed a prospective study of patients presenting to the ED after an accident. Patients with head injuries but without obvious ocular trauma or ocular disease were included. The ONUS was performed using a 3 to 12 MHz linear probe on closed eyelids after a primary survey. We analyzed the correlation between the brain computed tomography (CT) findings that suggested elevated intracranial pressure (ICP) and the Optic nerve sheath diameter (ONSD) measured by using ONUS. Results: A total of 81 patients were enrolled. Forty-seven had CT results consistent with elevated ICP, and their mean ONSD was $5.98{\pm}0.59$ mm; the mean ONSD of patients who showed no signs of elevated ICP on CT was $4.63{\pm}0.21$ mm. The sensitivity and the specificity for the ONSD, compared with elevated ICP, were 98.87% and 100%, respectively, when the cut-off value was set to 4.96 mm. The area under curve (AUC) was 0.997 in the receiver operating characteristic curve (ROC curve). Conclusion: An evaluation using ONUS is a simple noninvasive procedure and is a potentially useful tool as a secondary survey to identify an elevated ICP.

Study on Hwa-acupuncture Theory (오행화침법(五行和鍼法)에 대한 연구 - 부방(腑方)중심으로)

  • Sim, Sung-Heum;Kam, Cheol-Woo;Park, Dong-Il;Byun, Mi-Kwon;Kim, Sang-Heon;Baek, Sang-In
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.5
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    • pp.1119-1124
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    • 2008
  • The theory of Hwa Acupuncture Therapy (HAT), called Hwa Chim Therapy, is a part of the Five Elements Theory unique to Korea. H99AT, created and developed by Jaehoon Song, integrates the victor-vanquished as well as the son-mother relationship of the Five Elements of breakdown and restoration of balance between yin and yang. And also, it provides resources and data on The seventy fifth Difficulty Nan(75難), The sixty ninth Difficulty Nan(69難) of Classic on Difficulty - Nan Jin 75, 69. HAT establishes objectiveness and accuracy of diagnosis based upon the traditional method and procedure of pulse taking. In HAT, a person's state of illness is diagnosed by applying the comparative examination of the palpitation of the pulse. It is the fact that the pulse varies according to the state, and that HAT treatment has proven the positive results by using the victor-vanquished relationship of Classic on The Nan Jin 75. On the basis of this, it is necessary to add the sixty ninth Difficulty(69難), to research the theory of the generation of the Five Element. Despite a the concise and simple theory, Hwa Chim is very effective in treating the wide range of illness, and thus it has gained an increasing attention of many scholars and practitioners in the field of traditional Korean oriental medicine. However, it is the first theoretical attempt to the clinical research and scientific methodology of Ohaeng(Five) Hwa Chim, and more active Hwa Acupuncture R&D is being conducted nationwide.

Development of A Device Constantly Stimulating Tuning Fork and Variability of Its Vibration Perception Time (음차를 일정하게 자극하는 장치의 개발 및 이 장치로 측정한 진동 감지 시간의 변이)

  • Lee, Jong-Young;Hong, Dae-Yong;Yoon, Hyeong-Ryeol
    • Journal of Preventive Medicine and Public Health
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    • v.24 no.1 s.33
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    • pp.93-97
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    • 1991
  • The time honoured tuning fork at present widely available for examining vibration sensation is brought about the problem of great interobserver variation. To resolve t his problem, author developed a divice using electric magnet that stimulates constantly tuning fork. The perception time of vibration from tunung fork by this device was tested on the index finger of dominant hand of twenty eight subjects. It was 12.44 seconds on average and ranged from 9.47 to 17.25. Coefficient of variation of it was 16.89 percent. Correlation coefficient between test and retest after 30 minutes was 0.957(p<0.01). This device is portable. Test procedure in non-invasive, non-aversive and simple, can be performed within one minute, and does not require the skilled technician. It is felt that this device testing vibration perception time is suitable as screening tool for early detection of occupational peripheral neuropathy.

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Development of an Efficient Mass-screening Method for Testing the Resistance of Radish to Fusarium Wilt (무 시들음병에 대한 간편한 대량 저항성 검정법 개발)

  • Lee, Ji Hyun;Jang, Kyoung Soo;Choi, Yong Ho;Kim, Hun;Choi, Gyung Ja
    • Research in Plant Disease
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    • v.22 no.3
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    • pp.152-157
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    • 2016
  • Root-dipping inoculation method has been used to investigate resistance of radish plants to Fusarium wilt. However, the method requires a lot of labor and time because of complicate procedure. This study was conducted to establish a simple and effective mass-screening method for resistant radish to Fusarium wilt. Radish seedlings of susceptible and resistant cultivars were used to investigate wounding method by scalpel, inoculum concentration, and pathogen-inoculated growth stage of seedlings. We established an efficient mass-screening method based on our results as following: Roots of 14-day-old seedlings of radish are cut with a scalpel at a $90^{\circ}$ angle to a 2 cm-depth at a 1 cm-distance from main stem and then inoculated by pouring with a 10 ml-aliquot of a fungal spore suspension ($1.0{\times}10^7conidia/ml$) on soil. The inoculated plants are cultivated in a growth room at $25^{\circ}C$ for about 4 weeks with 12-hour light a day. The proposed screening method enables to effectively select resistant from mass radish plants cultivars to Fusarium wilt.

Verification of Spatial Resolution in DMC Imagery using Bar Target (Bar 타겟을 이용한 DMC 영상의 공간해상력 검증)

  • Lee, Tae Yun;Lee, Jae One;Yun, Bu Yeol
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.30 no.5
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    • pp.485-492
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    • 2012
  • Today, a digital airborne imaging sensor plays an important role in construction of the numerous National Spatial Data Infrastructure. However, an appropriate quality assesment procedure for the acquired digital images should be preceded to make them useful data with high precision and reliability. A lot of studies therefore have been conducted in attempt to assess quality of digital images at home and abroad. In this regard, many test fields have been already established and operated to calibrate digital photogrammetric airborne imaging systems in Europe and America. These test fields contain not only GCPs(Ground Control Points) to test geometric performance of a digital camera but also various types of targets to evaluate its spatial and radiometric resolution. The purpose of this paper is to present a method to verify the spatial resolution of the Intergraph DMC digital camera and its results based on an experimental field testing. In field test, a simple bar target to be easily identified in image is used to check the spatial resolution. Images, theoretically designed to 12cm GSD(Ground Sample Distance), were used to calculate the actual resolution for all sub-images and virtual images in flight direction as well as in cross flight direction. The results showed that the actual image resolution was about 0.6cm worse than theoretically expected resolution. In addition, the greatest difference of 1.5cm between them was found in the image of block edge.

Distinguishing the Korean Silage Corn Varieties through Development of PCR-Based SNP Marker (SNP마커 개발을 통한 사료용 옥수수 품종판별)

  • Kim, Sang Gon;Lee, Jin-Seok;Bae, Hwan Hee;Kim, Jung-Tae;Son, Beom-Young;Baek, Seong-Bum
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.2
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    • pp.168-175
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    • 2017
  • Single nucleotide polymorphisms (SNP) markers allow rapid screening of crop varieties in early growth stages. We developed a modified SNP PCR procedure for assaying SNPs in maize. For SNP marker development, we chosen 200 SNP sites from MaizeGDB database, and designed two base pair mismatch primers based on putative SNP site of B73 genome sequence. PCR products size was from 200 to 500 bp or was not shown in the case of SNP site existing in Korean silage corns. Using previously discovered 16 primer sets, we investigated distinctness of 50 silage F1 hybrid corns including 10 Korean silage corns developed by RDA such as Gangdaok, Kwangpyeongok, Dapyeongok, Andaok, Yanganok, Singwangok, Jangdaok, Cheongdaok, Pyeonggangok, and Pyeonganok as well as 40 foreign commercial silage corns. From cluster analysis, we confirmed that 10 Korean silage F1 hybrid corns were clearly distinguished except for Singwangok, P1395, and several foreign commercial corns, and selected minimum SNP primer combination for Gangdaok, Jangdaok, Pyeonggangok, and Pyeonganok. Therefore, development of SNP marker sets might be faster, cheaper, and feasible breed discrimination method through simple PCR and agarose gel electrophoresis.

Estimation of Fire Dynamics Properties for Charring Material Using a Genetic Algorithm (유전 알고리즘을 이용한 탄화 재료의 화재 물성치 추정)

  • Chang, Hee-Chul;Park, Won-Hee;Lee, Duck-Hee;Jung, Woo-Sung;Son, Bong-Sei;Kim, Tae-Kuk
    • Fire Science and Engineering
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    • v.24 no.2
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    • pp.106-113
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    • 2010
  • Fire characteristics can be analyzed more realistically by using more accurate material properties related to the fire dynamics and one way to acquire these fire properties is to use one of the inverse property analyses. In this study the genetic algorithm which is frequently applied for the inverse heat transfer problems is selected to demonstrate the procedure of obtaining fire properties of the solid charring material with relatively simple chemical structure. The thermal decomposition on the surface of the test plate is occurred by receiving the radiative energy from external heat sources, and in this process the heat transfer through the test plate can be simplified by an unsteady 1-D problem. The inverse property analysis based on the genetic algorithm is then applied for the estimation of the properties related to the reaction pyrolysis. The input parameters for the analysis are the surface temperature and mass loss rate of the char plate which are determined from the unsteady 1-D analysis with a givenset of 8 properties. The estimated properties using the inverse analysis based on the genetic algorithm show acceptable agreements with the input properties used to obtain the surface temperature and mass loss rate with errors between 1.8% for the specific heat of the virgin material and 151% for the specific heat of the charred material.