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http://dx.doi.org/10.5423/RPD.2016.22.3.152

Development of an Efficient Mass-screening Method for Testing the Resistance of Radish to Fusarium Wilt  

Lee, Ji Hyun (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology)
Jang, Kyoung Soo (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology)
Choi, Yong Ho (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology)
Kim, Hun (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology)
Choi, Gyung Ja (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology)
Publication Information
Research in Plant Disease / v.22, no.3, 2016 , pp. 152-157 More about this Journal
Abstract
Root-dipping inoculation method has been used to investigate resistance of radish plants to Fusarium wilt. However, the method requires a lot of labor and time because of complicate procedure. This study was conducted to establish a simple and effective mass-screening method for resistant radish to Fusarium wilt. Radish seedlings of susceptible and resistant cultivars were used to investigate wounding method by scalpel, inoculum concentration, and pathogen-inoculated growth stage of seedlings. We established an efficient mass-screening method based on our results as following: Roots of 14-day-old seedlings of radish are cut with a scalpel at a $90^{\circ}$ angle to a 2 cm-depth at a 1 cm-distance from main stem and then inoculated by pouring with a 10 ml-aliquot of a fungal spore suspension ($1.0{\times}10^7conidia/ml$) on soil. The inoculated plants are cultivated in a growth room at $25^{\circ}C$ for about 4 weeks with 12-hour light a day. The proposed screening method enables to effectively select resistant from mass radish plants cultivars to Fusarium wilt.
Keywords
Breeding; Disease resistance; Fusarium oxysporum f. sp. raphani; Inoculation method; Screening;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
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