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A Thermostable Protease Produced from Bacillus sp. JE 375 Isolated from Korean Soil (한국의 토양으로부터 내열성 단백질 분해효소를 생산하는 Bacillus sp. JE 375의 선별)

  • Kim, Ji-Eun;Bai, Dong-Hoon
    • Korean Journal of Food Science and Technology
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    • v.38 no.3
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    • pp.419-426
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    • 2006
  • A thermophilic microorganism, strain JE 375, which produces a thermostable protease, was isolated from soil and compost in Korea. This gram-positive, rod-shaped, catalase positive, motility positive, and hemolysis ${\beta}$ containing organism was implicated in glucose fermentation, mannitol fermentation, xylose oxidation, aerobic activity and spore formation. The color of the colony was yellowish white. The temperature range for growth at pH 6.5 was between 55 and $70^{\circ}C$, with an optimum growth temperature of $65^{\circ}C$. This result confirmed the strain JE 375 as a thermophilic microorganism. The enzyme was produced aerobically at $65^{\circ}C$ during 20 hr in a medium (pH 6.5) containing 1% trypton. 1% maltose, 0.5% yeast extract and 1% NaCl. The 16S rDNA of strain JE 375 had 97.6% sequence similarity with the 16S rDNA of Bacillus caldoxyloyticus. On the basis of biochemical and physiological properties and phylogenetic analysis, we named the isolated strain as Bacillus sp. JE 375. The thermostable protease from Bacillus sp. JE 375 had been partially purified and characterized. The molecular weight of the enzyme was deduced from SDS-PAGE and gel chromatography as 55 kDa and its optimal temperature was $60^{\circ}C$. The enzyme showed its highest activity at pH 7.5 and was stable from pH 7.0 to 8.0.

Development of Functional Modified Atmosphere Film for Winter Date (중국산 대추(Winter date)의 신선도 유지를 위한 기능성 MA필름 개발)

  • Park Hyung-Woo;Junfeng Guan;Kim Sang-Hee;Cha Hwan-Soo;Park Hye-Ran;Kim Yoon-Ho
    • Food Science and Preservation
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    • v.13 no.2
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    • pp.125-130
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    • 2006
  • In order to study the storage stability of winter date (Zizyphus jujuba Miller) with functional MA film storage, linear low density polyethylene $(LLDPE,\;30{\mu}m\;and\;60{\mu}m)$ film packaging and non-packaging were compared $30{\mu}m$ LLDPE packaging, $60{\mu}m$ LLDPE packaging and non-packaging samples were stored at $0^{\circ}C,\;8^{\circ}C$ (low temperature) and $25^{\circ}C$(room temperature) for 10 weeks. Weight loss of $30{\mu}m$ LLDPE packaging, $60{\mu}m$ LLDPE packaging and non-packaging in room temperature were decreased by 3.6% 0.4% and 36.1% respectively. Titratable acidity showed the trend of similarity in all treatment during the storage conditions, and especially LLDPE film packaging showed higher than non-packaging in the last storage. Soluble solids showed an increase in non-packaging, a decrease in LDPE film packaging highly. Vitamin C content showed the trend of decrease in all treatments, but non-packed jujubes sustained higher than LLDPE film packaging.

Information Hiding Technique in Smart Phone for the Implementation of GIS Web-Map Service (GIS 웹 맵 서비스 구현을 위한 스마트 폰에서의 정보은닉 기법)

  • Kim, Jin-Ho;Seo, Yong-Su;Kwon, Ki-Ryong
    • Journal of Korea Multimedia Society
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    • v.13 no.5
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    • pp.710-721
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    • 2010
  • Recently, for the advancement of embedded technology about mobile device, a new kind of service, mash-up is appeared. It is service or application combining multimedia content making tool or device and web-GIS(geographic information system) service in the mobile environment. This service can be ease to use for casual user and can apply in various ways. So, It is served in web 2.0 environment actively. But, in the mashup service, because generated multimedia contents linked with web map are new type of multimedia contents which include user's migration routes in the space such as GPS coordinates. Thus, there are no protection ways for intellectual property created by GIS web-map service users and user's privacy. In this paper, we proposed a location and user information hiding scheme for GIS web-map service. This scheme embeds location and user information into a picture that is taken by camera module on the mobile phone. It is not only protecting way for user's privacy but is also tracing way against illegal photographer who is peeping person through hidden camera. And than, we also realized proposed scheme on the mobile smart phone. For minimizing margin of error about location coordinate value against contents manipulating attacks, GPS information is embedded into chrominance signal of contents considering weight of each digit about binary type of GPS coordinate value. And for tracing illegal photographer, user information such as serial number of mobile phone, phone number and photographing date is embedded into frequency spectrum of contents luminance signal. In the experimental results, we confirmed that the error of extracted information against various image processing attacks is within reliable tolerance. And after file format translation attack, we extracted embedded information from the attacked contents without no damage. Using similarity between extracted one and original templete, we also extracted whole information from damaged chrominance signal of contents by various image processing attacks.

IAA-Producing Penicillium sp. NICS01 Triggers Plant Growth and Suppresses Fusarium sp.-Induced Oxidative Stress in Sesame (Sesamum indicum L.)

  • Radhakrishnan, Ramalingam;Shim, Kang-Bo;Lee, Byeong-Won;Hwang, Chung-Dong;Pae, Suk-Bok;Park, Chang-Hwan;Kim, Sung-Up;Lee, Choon-Ki;Baek, In-Youl
    • Journal of Microbiology and Biotechnology
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    • v.23 no.6
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    • pp.856-863
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    • 2013
  • Application of rhizospheric fungi is an effective and environmentally friendly method of improving plant growth and controlling many plant diseases. The current study was aimed to identify phytohormone-producing fungi from soil, to understand their roles in sesame plant growth, and to control Fusarium disease. Three predominant fungi (PNF1, PNF2, and PNF3) isolated from the rhizospheric soil of peanut plants were screened for their growth-promoting efficiency on sesame seedlings. Among these isolates, PNF2 significantly increased the shoot length and fresh weight of seedlings compared with controls. Analysis of the fungal culture filtrate showed a higher concentration of indole acetic acid in PNF2 than in the other isolates. PNF2 was identified as Penicillium sp. on the basis of phylogenetic analysis of ITS sequence similarity. The in vitro biocontrol activity of Penicillium sp. against Fusarium sp. was exhibited by a 49% inhibition of mycelial growth in a dual culture bioassay and by hyphal injuries as observed by scanning electron microscopy. In addition, greenhouse experiments revealed that Fusarium inhibited growth in sesame plants by damaging lipid membranes and reducing protein content. Co-cultivation with Penicillium sp. mitigated Fusarium-induced oxidative stress in sesame plants by limiting membrane lipid peroxidation, and by increasing the protein concentration, levels of antioxidants such as total polyphenols, and peroxidase and polyphenoloxidase activities. Thus, our findings suggest that Penicillium sp. is a potent plant growth-promoting fungus that has the ability to ameliorate damage caused by Fusarium infection in sesame cultivation.

Replacement of Imported Timothy Hay with Domestic Italian Ryegrass Silage in a Horse Feedstuff (말에서 국내산 이탈리안 라이그라스 사일리지의 수입산 티모시 건초 대체 효과)

  • Kim, Ji Yung;Son, Jin;Lee, Bae Hun;Kim, Byong Wan;Sung, Kyung Il
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.40 no.1
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    • pp.44-49
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    • 2020
  • This study was conducted to investigate the possibility of replacing imported Timothy hay (TH) with domestic Italian ryegrass silage (IRGS) as a horse feed considering feed quality, nutrient digestibility and feed price. Two experimental diets (TH and IRGS) were fed to six-headed Thoroughbred (body weight, 475.7±33.3kg) of the Korea Racing Authority of Wondang Stud Farm. The 3 head animals were assigned to Control group (TH) and Treatment group (IRGS), respectively. The nutrient digestibility was determined by the total collection method. IRGS is enough for using as a horse feed because its Relative feed value(RFV) was higher than TH and its fermentation quality is suitable for horses. Although no difference was observed in nutrient digestibility, Total digestible nutrients(TDN), and Digestible energy(DE) between Control and Treatment group (p>0.05), the fact that price of IRGS was much lower (53.7~62.4%) than that of TH indicates IRGS has competitive advantage over TH as a horse forage feed. The present study indicates that IRGS can be fully replaced with TH due to its superior economic value even though the similarity of its nutrient digestibility, TDN, and DE to TH.

The Methodology for Extraction of Geochemical Anomalies, Using Regression Formula: an Example from a Granitic Body in Gyeonggi Province (회귀 수식을 이용한 지구화학적 이상분포지역 도출기법: 경기도화강암의 예)

  • 황상기;신성천;염승준;문상원
    • Economic and Environmental Geology
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    • v.35 no.2
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    • pp.137-147
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    • 2002
  • Natural geological and environmental processes reflect to element abundances in geological materials on the surface. This study aims to elucidate a possibility of geostatistical application to differentiate geochemical anomalies affected by anthropogenic and geogenic factors. A regional geochemical map was produced using 'inverse distance weight interpolation' method for analytical results of stream sediments «150 11m) which were collected from 2,290 first- to second-order streams over the whole Gyeonggi Province. The Jurassic granitic batholith in the southeastern province was selected as a target for the geostatistical examination. Factor analysis was conducted using 22 elements for stream sediments from 445 drainage basins over the granitic body. Co, Cr, Sc, MgO, Fe$_{2}$O$_{3}$, V, and Ni were grouped with high correlation coefficients and the depletion of the components may reflect the whole-rock chemistry of the granite. Regression analysis was done using Co, Cr, and Sc as dependent variables and other six components as independent variables, and the results were drawn as maps. The maps acquired generally show quite similar distribution patterns with those of concentrations of each variable. The similarity in the spatial patterns between the two maps indicates that the application of regression statistics can be valid for the interpretation of regional geochemical data. However, some components show local discrepancies which may be influenced by secondary factors regardless of the basement lithology. The regression analysis may be effective in extracting local geochemical anomalies which may reflect rather anthropogenic pollutions than geogenic influences.

An ESDA Tool for Time-series Spatial Association (지역분석을 위한 시계열 공간연관성 탐색도구)

  • Ahn Jae-Seong;Park Key-Ho;Lee Yang-Won
    • Spatial Information Research
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    • v.14 no.1 s.36
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    • pp.163-176
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    • 2006
  • The concept of 'spatial association' explains spatial distribution pattern of geographical phenomenon based on similarity with neighborhoods, as in the Tobler's Law of Geography: 'Everything is related to everything else, but near things are more related than distant things.' In this study, we develop a time-series exploratory analysis tool for discovering temporal patterns of spatial association by combining spatial statistics and geo-visualization, and thus present a possibility to support spatial decision-making process. As for the spatial proximity weight matrix indispensable to measuring global and local spatial association, we employ a variety of flexible weighting schemes using geometric characteristics of areal unit. In addition, we renovate the existing visualization methods for more effective understanding of the procedures and results of time-series analysis on spatial association: for instance, temporal parallel coordinate plot with box plot, animated map for spatial association, and 3D Moran scatterplot. The feasibility of our system is verified by time-series analysis experiments on the spatial association of land price fluctuation rate for all administrative units in Korea, $1995{\sim}2004$.

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Gas Chromatographic and Mass Spectrometric Determination of Alcohol Homologues in the Korean Folk Sojues (Distilled Liquor) (기체크로마토그래피 및 질량분석법에 의한 민속 소주중의 알코올 동족체 분석)

  • Lee, Dong Seon;Park, Hye Seong;Kim, Geon;Lee, Taek Su;No, Bong Su
    • Journal of the Korean Chemical Society
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    • v.38 no.9
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    • pp.640-652
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    • 1994
  • This work is undertaken to determine alcohol homologues so-called fusel oil that may be present in the Korean folk sojues (distilled liquor) made from grains and to describe sample preparation and analytical method by GC-FID-MS. Solid phase extraction method for sample preparation by using porous styrene divinyl benzene polymer (Porapak Q) was compared with steam distillation and solvent extraction method. Retention behaviors of homologous series of alcohols were also studied. Log values of retention time, molecular weight, boiling point, and capacity factor of alcohols showed linear correlations to the carbon number of an alcohol, to the oven temperature, and to the dielectric constant. Components such as methyl alcohol, n-propyl alcohol, isobutyl alcohol, isopentyl alcohol, and phenethyl alcohol have been identified. The more amount of isopentyl alcohol than other alcohols are contained in the Korean folk sojues, while that of n-propyl alcohol are contained in Chinese kaoliangchiew. Degree of similarity or dissimilarity and classification of the individual samples were discussed using multivariate statistical analysis(principal components analysis) based on GC data.

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Group Decision Making for New Professor Selection Using Fuzzy TOPSIS (퍼지 TOPSIS를 이용한 신임교수선택을 위한 집단의사결정)

  • Kim, Ki-Yoon;Yang, Dong-Gu
    • Journal of Digital Convergence
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    • v.14 no.9
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    • pp.229-239
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    • 2016
  • The aim of this paper is to extend the TOPSIS(Technique for Order Performance by Similarity to Ideal Solution) to the fuzzy environment for solving the new professor selection problem in a university. In order to achieve the goal, the rating of each candidate and the weight of each criterion are described by linguistic terms which can be expressed in trapezoidal fuzzy numbers. In this paper, a vertex method is proposed to calculate the distance between two trapezoidal fuzzy numbers. According to the concept of the TOPSIS, a closeness coefficient is defined to determine the ranking order of all candidates. This research derived; 1) 4 evaluation criteria(research results, education and research competency, personality, major suitability) for new professor selection, 2) the 5 step procedure of the proposed fuzzy TOPSIS method for the group decision, 3) priorities of 4 candidates in the new professor selection case. The results of this paper will be useful to practical expert who is interested in analyzing fuzzy data and its multi-criteria decision-making tool for personal selection problem in personal management. Finally, the theoretical and practical implications of the findings were discussed and the directions for future research were suggested.

Effect of Paprika and Zooplankton Supplementation in the Diets on Carotenoid Deposition and Skin Pigmentation of the Rose Bitterling Rhodeus ocellatus (사료내 Paprika와 동물플랑크톤 혼합첨가가 흰줄납줄개 Rhodeus ocellatus의 Carotenoid 축적 및 체색에 미치는 영향)

  • Lee, Choong-Ryul;Bae, Ki-Min;Lee, Sang-Min
    • Journal of Aquaculture
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    • v.22 no.1
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    • pp.100-104
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    • 2009
  • This study was conducted to evaluate the effect of dietary supplementation of paprika powder as carotenoid source with three different zooplankton on carotenoid deposition and skin pigmentation of rose bitterling Rhodeus ocellatus. Two replicate groups of fish (initial average weight 0.8 g) were fed the five experimental diets containing 5% paprika (P5) and mixture of 5% paprika with 3% three zooplankton of water flea Moina macrocopa (P5M3), Artemia nauplius Artemia sp. (P5A3) and rotifer Brachionus plicatilis (P5R3) and without supplementation of carotenoid source (Con) for 10 weeks. Survival of fish among the treatments was not affected by dietary supplementation. Total carotenoid contents of whole body in fish fed the diets containing 3% zooplankton was higher than that of fish fed other diets. Especially, the highest total carotenoid contents of whole body was observed in fish fed the P5A3 diet. Similarity, Lightness, $a^*$ and $b^*$ values of the fish fed the diets containing 3% zooplankton tended to be higher than that in fish fed control and P5 diets. The apparent color intensity of skin and fin in fish fed the diets containing paprika with Artemia nauplius and rotifer was better than those of fish fed other diets. The results of this study suggest that dietary supplementation of paprika with Artemia nauplius and rotifer can improve skin pigmentation of rose bitterling.