Browse > Article
http://dx.doi.org/10.4014/jmb.1209.09045

IAA-Producing Penicillium sp. NICS01 Triggers Plant Growth and Suppresses Fusarium sp.-Induced Oxidative Stress in Sesame (Sesamum indicum L.)  

Radhakrishnan, Ramalingam (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA)
Shim, Kang-Bo (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA)
Lee, Byeong-Won (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA)
Hwang, Chung-Dong (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA)
Pae, Suk-Bok (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA)
Park, Chang-Hwan (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA)
Kim, Sung-Up (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA)
Lee, Choon-Ki (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA)
Baek, In-Youl (Legume and Oil Crop Research Division, Department of Functional Crop, National Institute of Crop Science, RDA)
Publication Information
Journal of Microbiology and Biotechnology / v.23, no.6, 2013 , pp. 856-863 More about this Journal
Abstract
Application of rhizospheric fungi is an effective and environmentally friendly method of improving plant growth and controlling many plant diseases. The current study was aimed to identify phytohormone-producing fungi from soil, to understand their roles in sesame plant growth, and to control Fusarium disease. Three predominant fungi (PNF1, PNF2, and PNF3) isolated from the rhizospheric soil of peanut plants were screened for their growth-promoting efficiency on sesame seedlings. Among these isolates, PNF2 significantly increased the shoot length and fresh weight of seedlings compared with controls. Analysis of the fungal culture filtrate showed a higher concentration of indole acetic acid in PNF2 than in the other isolates. PNF2 was identified as Penicillium sp. on the basis of phylogenetic analysis of ITS sequence similarity. The in vitro biocontrol activity of Penicillium sp. against Fusarium sp. was exhibited by a 49% inhibition of mycelial growth in a dual culture bioassay and by hyphal injuries as observed by scanning electron microscopy. In addition, greenhouse experiments revealed that Fusarium inhibited growth in sesame plants by damaging lipid membranes and reducing protein content. Co-cultivation with Penicillium sp. mitigated Fusarium-induced oxidative stress in sesame plants by limiting membrane lipid peroxidation, and by increasing the protein concentration, levels of antioxidants such as total polyphenols, and peroxidase and polyphenoloxidase activities. Thus, our findings suggest that Penicillium sp. is a potent plant growth-promoting fungus that has the ability to ameliorate damage caused by Fusarium infection in sesame cultivation.
Keywords
Antioxidants; biocontrol; Fusarium; plant growth; Penicillium;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.   DOI   ScienceOn
2 Chutima, R. and S. Lumyong. 2012. Production of indole-3- acetic acid by Thai native orchid-associated fungi. Symbiosis 56: 35-44.   DOI
3 El-Bramawy, M. A. S. 2006. Inheritance of resistance to Fusarium wilt in some crosses under field conditions. Plant Prot. Sci. 42: 99-105.
4 Gajbhiye, A., A. R. Rai, S. U. Meshram, and A. B. Dongre. 2010. Isolation, evaluation and characterization of Bacillus subtilis from cotton rhizospheric soil with biocontrol activity against Fusarium oxysporum. World J. Microbiol. Biotechnol. 26: 1187-1194.   DOI   ScienceOn
5 Gill, S. S. and N. Tuteja. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem. 48: 909-930.   DOI   ScienceOn
6 Hariprasad, P., S. T. Divakara, and S. R. Niranjana. 2011. Isolation and characterization of chitinolytic rhizobacteria for the management of Fusarium wilt in tomato. Crop Prot. 30: 1606-1612.   DOI   ScienceOn
7 Hildebrandt, U., K. Janetta, and H. Bothe. 2002. Towards growth of arbuscular mycorrhizal fungi independent of a plant host. Appl. Environ. Microbiol. 68: 1919-1924.   DOI   ScienceOn
8 Hasan, H. A. H. 2002. Gibberellin and auxin-indole production by plant root-fungi and their biosynthesis under salinity-calcium interaction. Acta Microbiol. Immunol. Hung. 49: 105-118.   DOI   ScienceOn
9 Heller, J. and P. Tudzynski. 2011. Reactive oxygen species in phytopathogenic fungi: Signaling, development, and disease. Annu. Rev. Phytopathol. 49: 369-390.   DOI   ScienceOn
10 Heydari, A. and M. Pessarakli. 2010. A review on biological control of fungal plant pathogens using microbial antagonists. J. Biol. Sci. 10: 273-290.   DOI
11 Jetiyanon, K. 2007. Defensive-related enzyme response in plants treated with a mixture of Bacillus strains (IN937a and IN937b) against different pathogens. Biol. Control 42: 178-185.   DOI   ScienceOn
12 Jyothi, B., N. A. Ansari, Y. Vijay, G. Anuradha, A. Sarkar, R. Sudhakar, and E. A. Siddiq. 2011. Assessment of resistance to Fusarium wilt disease in sesame (Sesamum indicum L.) germplasm. Aust. Plant Pathol. 40: 471-475.   DOI   ScienceOn
13 Kar, M. and D. Mishra. 1976. Catalase, peroxidase and polyphenoloxidase activities during rice leaf senescence. Plant Physiol. 57: 315-319.   DOI   ScienceOn
14 Khamna, S., A. Yokota, and S. Lumyong. 2009. Actinomycetes isolated from medicinal plant rhizosphere soils: Diversity and screening of antifungal compounds, indole-3-acetic acid and siderophore production. World J. Microbiol. Biotechnol. 25: 649-655.   DOI   ScienceOn
15 Kumazawa, S., T. Hamasaka, and T. Nakayama. 2004. Antioxidant activity of propolis of various geographic origins. Food Chem. 84: 329-339.   DOI   ScienceOn
16 Khan, A. L., M. Hamayun, N. Ahmad, J. Hussain, S. M. Kang, Y. H. Kim, et al. 2011. Salinity stress resistance offered by endophytic fungal interaction between Penicillium minioluteum LHL09 and Glycine max. L. J. Microbiol. Biotechnol. 21: 893-902.   DOI   ScienceOn
17 Ksouri, R., W. Megdiche, A. Debez, H. Falleh, C. Grignon, and C. Abdelly. 2007. Salinity effects on polyphenol content and antioxidant activities in leaves of the halophyte Cakile maritime. Plant Physiol. Biochem. 45: 244-249.   DOI   ScienceOn
18 Kumar, S., P. Pandey, and D. K. Maheshwari. 2009. Reduction in dose of chemical fertilizers and growth enhancement of sesame (Sesamum indicum L.) with application of rhizospheric competent Pseudomonas aeruginosa LES4. Eur. J. Soil Biol. 45: 334-340.   DOI   ScienceOn
19 Latha, P., T. Anand, V. Prakasama, E. I. Jonathan, M. Paramathma, and R. Samiyappan. 2001. Combining Pseudomonas, Bacillus and Trichoderma strains with organic amendments and micronutrient to enhance suppression of collar and root rot disease in physic nut. Appl. Soil Ecol. 49: 215-223.
20 Manikandan, R., D. Saravanakumar, L. Rajendran, T. Raguchander, and R. Samiyappan. 2010. Standardization of liquid formulation of Pseudomonas fluorescens Pf1 for its efficacy against Fusarium wilt of tomato. Biol. Control 54: 83-89.   DOI   ScienceOn
21 Miransari, M. 2011. Soil microbes and plant fertilization. Appl. Microbiol. Biotechnol. 92: 875-885.   DOI   ScienceOn
22 Ohkawa, H., N. Ohishi, and K. Yagi. 1979. Assay of lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal. Biochem. 95: 351-358.   DOI   ScienceOn
23 Ruanpanun, P., N. Tangchitsomkid, K. D. Hyde, and S. Lumyong. 2010. Actinomycetes and fungi isolated from plant-parasitic nematode infested soils: Screening of the effective biocontrol potential, indole-3-acetic acid and siderophore production. World J. Microbiol. Biotechnol. 26: 1569-1578.   DOI
24 Pavlo, A., O. Leonid, Z. Iryna, K. Natalia, and P. A. Maria. 2011. Endophytic bacteria enhancing growth and disease resistance of potato (Solanum tuberosum L.). Biol. Control 56: 43-49.   DOI   ScienceOn
25 Peskan-Berghoefer, T., B. Shahollari, P. H. Giong, S. Hehl, C. Markert, V. Blanke, et al. 2004. Association of Piriformospora indica with Arabidopsis thaliana roots represents a novel system to study beneficial plant-microbe interactions and involves early plant protein modifications in the endoplasmic reticulum and at the plasma membrane. Physiol. Plant. 122: 465-477.   DOI   ScienceOn
26 Pimentel, D. and L. Levitan. 1986. Pesticides: Amounts applied and amounts reaching pests. Bioscience 36: 86-91.   DOI   ScienceOn
27 Shoresh, M., G. E. Harman, and F. Mastouri. 2010. Induced systemic resistance and plant responses to fungal biocontrol agents. Annu. Rev. Phytopathol. 48: 21-43.   DOI   ScienceOn
28 Sundaramoorthy, S., T. Raguchander, N. Ragupathi, and R. Samiyappan. 2012. Combinatorial effect of endophytic and plant growth promoting rhizobacteria against wilt disease of Capsicum annum L. caused by Fusarium solani. Biol. Control 60: 59-67.
29 Ting, A. S. Y., S. W. Mah, and C. S. Tee. 2012. Evaluating the feasibility of induced host resistance by endophytic isolate Penicillium citrinum BTFO8 as a control mechanism for Fusarium wilt in banana plantlets. Biol. Control 61: 155-159.   DOI   ScienceOn
30 Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28: 2731-2739.   DOI   ScienceOn
31 Vujanovic, V., D. Mavragani, and C. Hamel. 2012. Fungal communities associated with durum wheat production systems: A characterization by growth stage, plant organ and preceding crop. Crop Prot. 37: 26-34.   DOI   ScienceOn
32 Wu, H. S., X. N. Yang, J. Q. Fan, W. G. Miao, N. Ling, Y. C. Xu, et al. 2009. Suppression of Fusarium wilt of watermelon by a bio-organic fertilizer containing combinations of antagonistic microorganisms. Biol. Control 54: 287-300.
33 Alwathnani, H. A., K. Perveen, R. Tahmaz, and S. Alhaqbani. 2012. Evaluation of biological control potential of locally isolated antagonist fungi against Fusarium oxysporum under in vitro and pot conditions. Afr. J. Microbiol. Res. 6: 312-319.
34 Artursson, V., R. Finlay, and J. Jansson. 2006. Interactions between arbuscular mycorrhizal fungi and bacteria and their potential for stimulating plant growth. Environ. Microbiol. 8: 1-10.   DOI   ScienceOn
35 Alabouvette, C., C. Olivain, Q. Migheli, and C. Steinberg. 2009. Microbiological control of soil-borne phytopathogenic fungi with special emphasis on wilt-inducing Fusarium oxysporum. New Phytol. 184: 529-544.   DOI   ScienceOn
36 Alam, S. S., K. Sakamoto, and K. Inubushi. 2011. Biocontrol efficiency of Fusarium wilt diseases by a root-colonizing fungus Penicillium sp. Soil Sci. Plant Nutr. 57: 204-212.   DOI
37 Bacon, C. W. and D. M. Hinton. 2007. Potential for control of seedling blight of wheat caused by Fusarium graminearum and related species using the bacterial endophyte Bacillus mojavensis. Biocontrol Sci. Technol. 17: 81-94.   DOI   ScienceOn
38 De-Vrije, T., N. Antoine, R. M. Buitelaar, S. Bruckner, M. Dissevelt, A. Durand, et al. 2001. The fungal biocontrol agent Coniothyrium minitans: Production by solid-state fermentation, application and marketing. Appl. Microbiol. Biotechnol. 56: 58-68.   DOI   ScienceOn
39 Barazani, O., M. Benderoth, K. Groten, C. Kuhlemeier, and I. T. Baldwin. 2005. Piriformospora indica and Sebacina vermifera increase growth performance at the expense of herbivore resistance in Nicotiana attenuata. Oecologia 146: 234-243.   DOI