• The Journal of Korean Society of Virology
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• v.29 no.4
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• pp.221-233
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• 1999

Prospect Hill Virus (PHV) is the well known serotype of hantavirus, a newly established genus in family Bunyaviridae. Extensive studies have upheld the original view of PHV genetics with three genes such as nucleocapsid (N) protein, envelope proteins (G1, G2) and RNA dependent RNA polymerase. In this study, we report the existence of additional gene that is encoded in an overlapping reading frame of the N protein gene within S genome segment of PHV. This gene is expected to encode a nonstructural small (NSs) protein and it seems to be only found in PHV infected cell. The presence and synthesis of NSs protein could be demonstrated in the cell infected with PHV using anti-peptide sera specific to the predicted amino acid sequence deduced from the second open reading frame. Ribosomal synthesis of this protein appears to occur at AUG codon at the 83rd base of S genome segment, downstream of N protein initiation codon. This protein is small in size (10.4 KDa) and highly basic in nature. The expression strategy of NSs protein appears that a signal mRNA is used to translate both N and NSs protein in PHV infected cell. 10 KDa protein in virus infected cell lysates can bind to mimic dsRNA. This fact strongly suggests that NSs protein may be involved in virus replication on late phase of viral life cycle.

• Journal of Plant Biotechnology
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• v.39 no.4
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• pp.273-280
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• 2012

Differences of gene expression between red flash peach cultivar and white flash peach cultivar were investigated by Nest-generation sequencing (NGS). EST from the red flash peach cultivar and white flash peach cultivar were selected for nucleotide sequence determination and homology searches. The levels of transcripts coding for proteins involved in pathogenesis related proteins, temperature stress, ethylene signal pathway were significantly higher in white flash peach cultivar than in red flash peach cultivar. On the other hand, the up-regulation of proteins involved in anthocyanin and flavonol biosynthesis and protein degradation and sorbitol metabolism were observed in red flash peach cultivar. Chalcone synthase was preferentially expressed in the red flesh peach cultivar, agreeing with the accumulation of anthocyanin and expression of other previously identified genes for anthocyanin biosynthesis. Anthocyanin pathway related genes CHS, F3H, DFR, LDOX, UFGT differentially expressed between red flash peach cultivar and white flash peach cultivar. These results suggest that red flash peach cultivar and white flash peach cultivar have different anthocyanin biosynthesis regulatory mechanisms.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.4A
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• pp.330-335
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• 2002

In the direct sequence code division multiple access system, the problem of multiple access interference due to multiple access is generated. A interference cancelling receiver is used to solve this problem. The conventional interference cancelling receiver is structure of successive interference canceller using antenna array. In this structure, the difference of between method I and method II depends on updating weight vector. In this paper, the adaptive blind CMA array interference cancelling receiver using cost function of constant modulus algorithms is proposed to update weight vector at conventional structure. The simulation compared the proposed interference cancelling receiver with two conventional interference cancelling receivers by signal to interference ratio and bit error rate curve under additive white Gaussian noise environment. The simulation results show that the proposed receiver has about the gain of SIR of 1.5[dB] more than method I which is conventional receiver at SIR curve, and about the gain of SIR of 0.5(dB) more than method II. In BER curve, the proposed IC receiver about the gain of SNR of 2[dB] more than method I and about the gain of SNR of 0.5[dB] more than method If, Thus, the proposed interference cancelling receiver has the higher performance than conventional interference cancelling receivers.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.9A
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• pp.991-997
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• 2004

In this paper, we propose the architecture of modem receiver to fabricate HomePNA 2.0 chip. HomePNA suffers from inferior channel because of bridge tap, the effect of amateur HAM band and so on. To transfer data over such channel, HomePNA 2.0 uses training sequence to equalize channel and uses FD-QAM optionally as modulation method. So modem receiver demodulate QAM based signal and needs optimum architecture that fully uses these transmission feature. As a result of research, we define 2 mode function of modem receiver depending on TX/RX state. In this paper, particularly, we show the algorithm of equalizer, carrier phase recovery and frame synchromzationblock and propose architecture that improve the performance of channel equalization and is stable in operation. In the end, we estimate the performance of proposed HomePNA2.0 modem receiver over HomePNA TEST LOOP using SPW program.

• Korean Journal of Veterinary Research
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• v.44 no.2
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• pp.311-315
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• 2004

The detection of lymph node metastasis is an important step in tumor staging and is significant for therapy planning. It has been challenged to yield an appropriate image with diagnostic methods such as Magnetic Resonance (MR) and Computed Tomography (CT). Though CT has been used widely and accessed easily to show internal organs, it can hardly provide difference between lymph node and adjacent vessel or fat tissue. It has been well established that MR can reveal the subtle discrepancy within soft tissue. This study investigated the suitability of MR lymph node imaging without contrast enhancement by comparison of T1-weighted image (T1WI) and T2- weighted image (T2WI) in ten normal rabbits. According to the pulse sequence optimized from preliminary study, T1-weighted spin-echo (repetition time/echo time=400/12 ms) and T-2 weighted fast spin-echo (repetition time/echo time=3500/84 ms) images covering the hind limbs and pelvic region were acquired at 1.5 T. Two radiologists scrupulously evaluated the MR images in consensus. And signal intensity of lymph nodes was compared with that of adjacent fat. Statistical analysis showed that T1-weighted coronal image visualized the lymph nodes (iliac, superficial inguinal and popliteal lymph nodes) quickly and consistently rather than T2-weighted one. Conclusively, T1WI for evaluation of lymph nodes is moderately better than T2WI and appears to have potential for quick and sufficient mapping of the lymph nodes. In addition, this normal MR image of lymph nodes could be applied to further study for the evaluation of lymphatic system in abscess and tumor bearing animal model.

• Molecules and Cells
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• v.28 no.2
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• pp.131-137
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• 2009

Plant defensins are small (5-10 kDa) basic peptides thought to be an important component of the defense pathway against fungal and/or bacterial pathogens. To understand the role of plant defensins in protecting plants against the brown planthopper, a type of insect herbivore, we isolated the Brassica rapa Defensin 1 (BrD1) gene and introduced it into rice (Oryza sativa L.) to produce stable transgenic plants. The BrD1 protein is homologous to other plant defensins and contains both an N-terminal endoplasmic reticulum signal sequence and a defensin domain, which are highly conserved in all plant defensins. Based on a phylogenetic analysis of the defensin domain of various plant defensins, we established that BrD1 belongs to a distinct subgroup of plant defensins. Relative to the wild type, transgenic rices expressing BrD1 exhibit strong resistance to brown planthopper nymphs and female adults. These results suggest that BrD1 exhibits insecticidal activity, and might be useful for developing cereal crop plants resistant to sap-sucking insects, such as the brown planthopper.

• Plant Biotechnology Reports
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• v.4 no.1
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• pp.53-59
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• 2010

A specific deleted version of ARABIDOPSIS RESPONSE REGULATOR1 (ARR1) lacking the signal receiver domain (1.152 amino acids)-coding sequence, referred to as $ARR1{\Delta}DDK$, was amplified using Arabidopsis thaliana cDNA prepared from adult leaves and transferred into the genome of Nicotiana tabacum cv. Samsun under the transcriptional control of a ${\beta}$-estradiol-inducible expression system. The ectopic expression of $ARR1{\Delta}DDK$ affected the morphology of transgenic seedlings and their segments in vitro. In the presence of an inducer, ${\beta}$-estradiol, ectopic expression of $ARR1{\Delta}DDK$ induced only the formation of soft, pseudo-bulbous tissue in the root tip region of intact seedlings, which appeared similar to callus generated on a hypocotyl segment in the presence of 2,4-D and 6-benzyladenine (BA), both at $1\;{\mu}M$. Those callus tissues on the root tip region could not generate shoots unless $1\;{\mu}M$ BA was supplied. In segment culture, ectopic expression of $ARR1{\Delta}DDK$ induced calluslike tissue around the cut-end of cotyledon and hypocotyl segments with occasional shoot formation, suggesting that the expression of $ARR1{\Delta}DDK$ could substitute for the effects of cytokinin on these segments. Additionally, treatment with only ${\beta}$-estradiol induced NtWUS, a WUS ortholog in tobacco, which was detected during the process of callus tissue formation in the root tip region and also in cotyledon or hypocotyl segments. These findings suggest that the NtWUS might be associated in the transdifferentiation process caused by the functional regulation of $ARR1{\Delta}DDK$ in transgenic tobacco seedlings.

• Korean Journal of Veterinary Research
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• v.35 no.1
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• pp.95-105
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• 1995

The human homolog of position specific element of mouse Hoxa-7 was studied using transgene. It contains a 1.1 kb human DNA (HCR)- a homolog to the intergenic region between Hoxa-7 and -9, which directs the position specific expression of Hoxa-7-, tk promoter, LacZ (${\beta}$-galactosidase) gene as a reporter, and polyadenylation signal of SV40 large T antigen. It was injected into the mice embryos, and the resulting transgenic embryos were analysed through PCR as well as genomic Southern blotting with placenta DNA. Out of 20 embryos analysed, two were transgenic. Among them, one transgenic embryo expressed transgene when stained with X-gal. The expression pattern was in analogy to that of the mouse Hoxa-7, showing spatially restricted expression pattern, Since the expression of ${\beta}$-galactosidase is regulated by the upstream human HCR sequence, it implies that the HCR is the plausible position specific regulatory element of human.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.39 no.9
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• pp.848-857
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• 2011

Vision-based collision avoidance system for air traffic management requires a excellent multiple target detection algorithm under low signal-to-noise ratio (SNR) levels. The track-before-detect (TBD) approaches have significant applications such as detection of small and dim targets from an image sequence. In this paper, two detection algorithms with the TBD approaches are proposed to satisfy the multiple target detection requirements. The first algorithm, based on a dynamic programming approach, is designed to classify multiple targets by using a k-means clustering algorithm. In the second approach, a hidden Markov model (HMM) is slightly modified for detecting multiple targets sequentially. Both of the proposed approaches are used in numerical simulations with variations in target appearance properties to provide satisfactory performance as multiple target detection methods.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.9
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• pp.1884-1891
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• 2012

In this paper, we design the sliding correlator SS transceiver which supports short message service. We also analyze the PN code acquisition circuit that is essential for spread spectrum receiver. Using Maxplus II tool provided by altera Co., Ltd, we have designed PN code generator, and sliding correlator for PN code acquisition. Then, they have been made into FPGA by way of EPM7064SLC44-10 - a chip of Altera Co., Ltd. Additionally, we have designed delay clock circuit which is faster than the clock of Tx PN clock, designed switching circuit to control the clock rate and data demodulation circuit. The performance of the transceiver is evaluated from the experimental results. Especially, the performance of PN code acquisition accomplished by sliding correlator which is very important to evaluate spread spectrum receiver is evaluated with the comparison of the lock states.